Restriction map for
pFN21AE1263
(Circular) (Six-base) MAPSORT of: pFN21AE1263.seq Check: 1171 from: 1 to: 6526
pFN21AE1263 6526 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4336 278
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3869 1937
Size: 1932 4594
Acc65I G'GTAC_C
Cuts at: 2932 3847 2932
Size: 915 5611
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 3613 4589 5973 6241 1632
Size: 878 1103 976 1384 268 1917
Fragments arranged by size:
1917 1384 1103 976 878 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 5425 6402 1203
Size: 4222 977 1327
Fragments arranged by size:
4222 1327 977
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 2864 5255 2864
Size: 2391 4135
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3824 1184
Size: 2640 3886
AlwNI CAG_nnn'CTG
Cuts at: 2488 2572 2942 3662 4269 5841 2488
Size: 84 370 720 607 1572 3173
Fragments arranged by size:
3173 1572 720 607 370 84
ApaLI G'TGCA_C
Cuts at: 3585 4583 5739 3585
Size: 998 1156 4372
Fragments arranged by size:
4372 1156 998
ApoI r'AATT_y
Cuts at: 1493 3122 3414 3835 4061 4213 6322 1493
Size: 1629 292 421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1629 421 292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 3851 1870
Size: 73 1908 4545
Fragments arranged by size:
4545 1908 73
BamHI G'GATC_C
Cuts at: 3350 3856 4223 3350
Size: 506 367 5653
Fragments arranged by size:
5653 506 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2932 3847 5302 618
Size: 325 217 627 283 862 915 1455 1842
Fragments arranged by size:
1842 1455 915 862 627 325 283 217
BanII G_rGCy'C
Cuts at: 1391 3513 3674 3845 1391
Size: 2122 161 171 4072
Fragments arranged by size:
4072 2122 171 161
BbeI G_GCGC'C
Cuts at: 2074 2074
Size: 6526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2947 3619 928
Size: 971 1048 672 3835
Fragments arranged by size:
3835 1048 971 672
BbvCI CC'TCA_GC
Cuts at: 3666 3666
Size: 6526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4717 4751 1903
Size: 34 2780 34 3678
Fragments arranged by size:
3678 2780 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2893 4418 5628 2893
Size: 1525 1210 3791
Fragments arranged by size:
3791 1525 1210
BclI T'GATC_A
Cuts at: 1548 2749 1548
Size: 1201 5325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3639 5137 136
Size: 107 122 71 631 605 1967 1498 1525
Fragments arranged by size:
1967 1525 1498 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2143 6521 2143
Size: 4378 2148
BlpI GC'TnA_GC
Cuts at: 3943 3943
Size: 6526
Bme1580I G_kGCm'C
Cuts at: 1790 3589 3820 4587 5743 1790
Size: 1799 231 767 1156 2573
Fragments arranged by size:
2573 1799 1156 767 231
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2873 4257 5215
Size: 416 53 83 399 24 1456 1384 958
Cuts at: 5215 442
Size: 1753
Fragments arranged by size:
1753 1456 1384 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2447 2626 2841 3042 3706 5186 1239
Size: 1208 179 215 201 664 1480 2579
Fragments arranged by size:
2579 1480 1208 664 215 201 179
Bpu10I CC'TnA_GC
Cuts at: 3515 3666 3515
Size: 151 6375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4651 5516 5814 4651
Size: 865 298 5363
Fragments arranged by size:
5363 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2756 5189 882
Size: 617 1257 2433 2219
Fragments arranged by size:
2433 2219 1257 617
BsaAI yAC'GTr
Cuts at: 493 1204 3649 4317 493
Size: 711 2445 668 2702
Fragments arranged by size:
2702 2445 711 668
BsaBI GATnn'nnATC
Cuts at: 2904 3195 6520 2904
Size: 291 3325 2910
Fragments arranged by size:
3325 2910 291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2071 4333 4715 5350
Size: 53 83 186 936 538 2262 382 635
Cuts at: 5350 275
Size: 1451
Fragments arranged by size:
2262 1451 936 635 538 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3291 4959 5357 5631 5778 6189 3291
Size: 1668 398 274 147 411 3628
Fragments arranged by size:
3628 1668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 1178
Size: 30 165 30 1017 30 5254
Fragments arranged by size:
5254 1017 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 5595
BseYI C'CCAG_C
Cuts at: 5729 6225 5729
Size: 496 6030
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3008 3358 1163
Size: 573 1272 350 4331
Fragments arranged by size:
4331 1272 573 350
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 3976 4737 5765 1540
Size: 275 186 1291 684 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 684 275 186
BsiHKAI G_wGCw'C
Cuts at: 3513 3589 3696 4587 4672 5743 3513
Size: 76 107 891 85 1071 4296
Fragments arranged by size:
4296 1071 891 107 85 76
BsmI GAATG_Cn'
Cuts at: 4037 4130 4037
Size: 93 6433
BsmBI CGTCTCn'nnnn_
Cuts at: 2423 2851 2423
Size: 428 6098
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3513 3589 3674 3696 3820 3845 4587
Size: 399 1723 76 85 22 124 25 742
Cuts at: 4587 4672 5743 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1723 1071 742 399 124 85 85
76 25 22
BspEI T'CCGG_A
Cuts at: 6189 6189
Size: 6526
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 3881 844
Size: 1610 1427 3489
Fragments arranged by size:
3489 1610 1427
BsrBI CCG'CTC
Cuts at: 1394 1823 4413 1394
Size: 429 2590 3507
Fragments arranged by size:
3507 2590 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3367 3389 5021 5195 65
Size: 1183 2119 22 1632 174 1396
Fragments arranged by size:
2119 1632 1396 1183 174 22
BsrFI r'CCGG_y
Cuts at: 1700 1810 2079 5170 5357 1700
Size: 110 269 3091 187 2869
Fragments arranged by size:
3091 2869 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 2204 4586 5598 2204
Size: 2382 1012 3132
Fragments arranged by size:
3132 2382 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2396 3327 3662 794
Size: 1602 931 335 3658
Fragments arranged by size:
3658 1602 931 335
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 2575 1184
Size: 1391 5135
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 2587
Size: 6526
BstYI r'GATC_y
Cuts at: 1972 2143 3137 3310 3350 3856 4223 4608 4625
Size: 171 994 173 40 506 367 385 17
Cuts at: 4625 6077 6163 6175 6521 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 994 506 385 367 346 173
171 86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5974
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5787
Fragments arranged by size:
5787 683 56
BtsI GCAGTG_nn'
Cuts at: 4042 4836 4856 4042
Size: 794 20 5712
Fragments arranged by size:
5712 794 20
ClaI AT'CG_AT
Cuts at: 1554 3199 1554
Size: 1645 4881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2293 2328 529
Size: 35 1729 35 4727
Fragments arranged by size:
4727 1729 35 35
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2726 5533 809
Size: 507 1410 2807 1802
Fragments arranged by size:
2807 1802 1410 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3015 3973 4862
Size: 54 1425 50 275 18 1185 958 889
Cuts at: 4862 6491 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1185 958 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3973 1537
Size: 275 2161 4090
Fragments arranged by size:
4090 2161 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2553 2754 2875 4456 1353
Size: 1200 201 121 1581 3423
Fragments arranged by size:
3423 1581 1200 201 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5113 5487 5633 387
Size: 1064 138 3524 374 146 1280
Fragments arranged by size:
3524 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3511 3511
Size: 6526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2510 2626 2841 3042 3613 3706
Size: 393 815 63 116 215 201 571 93
Cuts at: 3706 4589 5186 5973 6241 1239
Size: 883 597 787 268 1524
Fragments arranged by size:
1524 883 815 787 597 571 393 268
215 201 116 93 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2820 2820
Size: 6526
EcoRI G'AATT_C
Cuts at: 3835 4213 3835
Size: 378 6148
FspI TGC'GCA
Cuts at: 2450 5032 2450
Size: 2582 3944
HaeII r_GCGC'y
Cuts at: 1678 2050 2074 5673 1678
Size: 372 24 3599 2531
Fragments arranged by size:
3599 2531 372 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2850 2882 3129 3161 3419
Size: 32 135 32 1278 32 247 32 258
Cuts at: 3419 3451 5167 5199 5241 5273 1373
Size: 32 1716 32 42 32 2626
Fragments arranged by size:
2626 1716 1278 258 247 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2980 3870 4116 669
Size: 988 281 1042 890 246 3079
Fragments arranged by size:
3079 1042 988 890 281 246
HindIII A'AGCT_T
Cuts at: 748 2456 3044 748
Size: 1708 588 4230
Fragments arranged by size:
4230 1708 588
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KasI G'GCGC_C
Cuts at: 2070 2070
Size: 6526
KpnI G_GTAC'C
Cuts at: 2936 3851 2936
Size: 915 5611
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5640 5824 1305
Size: 4335 184 2007
Fragments arranged by size:
4335 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 10
Size: 54 1425 343 1185 3519
Fragments arranged by size:
3519 1425 1185 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4484 4843 5002 518
Size: 648 3318 359 159 2042
Fragments arranged by size:
3318 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2040 2359 3942 4619 5767 6012 1606
Size: 421 13 319 1583 677 1148 245 2120
Fragments arranged by size:
2120 1583 1148 677 421 319 245 13
NarI GG'CG_CC
Cuts at: 2071 2071
Size: 6526
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5974
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 3884 5429 1132
Size: 97 2655 1545 2229
Fragments arranged by size:
2655 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6526
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 1108
Size: 1464 5062
PfoI T'CCnGG_A
Cuts at: 2215 2642 2215
Size: 427 6099
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PstI C_TGCA'G
Cuts at: 830 2088 2118 2514 2667 3878 830
Size: 1258 30 396 153 1211 3478
Fragments arranged by size:
3478 1258 1211 396 153 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
SacI G_AGCT'C
Cuts at: 3513 3513
Size: 6526
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2084 2114 2171 2510 2663 3874 5009
Size: 219 1039 30 57 339 153 1211 1135
Cuts at: 5009 5690 5881 826
Size: 681 191 1471
Fragments arranged by size:
1471 1211 1135 1039 681 339 219 191
153 57 30
SfoI GGC'GCC
Cuts at: 2072 2072
Size: 6526
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 3853 1872
Size: 1981 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4630 5531 5793 820
Size: 197 926 2687 901 262 1553
Fragments arranged by size:
2687 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3884 1132
Size: 2752 3774
SspI AAT'ATT
Cuts at: 5 52 4450 5
Size: 47 4398 2081
Fragments arranged by size:
4398 2081 47
StuI AGG'CCT
Cuts at: 1517 2826 1517
Size: 1309 5217
StyI C'CwwG_G
Cuts at: 513 1065 2529 3965 513
Size: 552 1464 1436 3074
Fragments arranged by size:
3074 1464 1436 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4553 4570 4723 4908 5353 325
Size: 1255 2973 17 153 185 445 1498
Fragments arranged by size:
2973 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4772 6484 96
Size: 274 80 33 51 1443 2795 1712 138
Fragments arranged by size:
2795 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 3722 3754 6486 6518 2420
Size: 32 1270 32 2732 32 2428
Fragments arranged by size:
2732 2428 1270 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6526
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3025 3748 3025
Size: 723 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6526
XmaI C'CCGG_G
Cuts at: 1870 3851 1870
Size: 1981 4545
XmnI GAAnn'nnTTC
Cuts at: 3714 4655 3714
Size: 941 5585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4334 276
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI