Restriction map for
pFN21AE1261
(Circular) (Six-base) MAPSORT of: pFN21AE1261.seq Check: 5029 from: 1 to: 6517
pFN21AE1261 6517 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4327 278
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3860 1937
Size: 1923 4594
Acc65I G'GTAC_C
Cuts at: 3210 3838 3210
Size: 628 5889
AclI AA'CG_TT
Cuts at: 1563 4644 5017 1563
Size: 3081 373 3063
Fragments arranged by size:
3081 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2398 4580 5964 6232 1632
Size: 766 2182 1384 268 1917
Fragments arranged by size:
2182 1917 1384 766 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6320
AflIII A'CryG_T
Cuts at: 1203 5416 6393 1203
Size: 4213 977 1327
Fragments arranged by size:
4213 1327 977
AgeI A'CCGG_T
Cuts at: 5348 5348
Size: 6517
AhdI GACnn_n'nnGTC
Cuts at: 2257 5246 2257
Size: 2989 3528
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6517
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6517
AlwNI CAG_nnn'CTG
Cuts at: 4260 5832 4260
Size: 1572 4945
ApaLI G'TGCA_C
Cuts at: 4574 5730 4574
Size: 1156 5361
ApoI r'AATT_y
Cuts at: 1493 2283 3032 3826 4052 4204 6313 1493
Size: 790 749 794 226 152 2109 1697
Fragments arranged by size:
2109 1697 794 790 749 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5071 160
Size: 872 4039 1606
Fragments arranged by size:
4039 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
AvaI C'yCGr_G
Cuts at: 1870 1943 3842 1870
Size: 73 1899 4545
Fragments arranged by size:
4545 1899 73
AvrII C'CTAG_G
Cuts at: 2176 3091 2176
Size: 915 5602
BamHI G'GATC_C
Cuts at: 3847 4214 3847
Size: 367 6150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3210 3241 3838 5293 618
Size: 325 217 627 1423 31 597 1455 1842
Fragments arranged by size:
1842 1455 1423 627 597 325 217 31
BanII G_rGCy'C
Cuts at: 1391 2396 2808 3836 1391
Size: 1005 412 1028 4072
Fragments arranged by size:
4072 1028 1005 412
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 928
Size: 971 354 5192
Fragments arranged by size:
5192 971 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2332 2366 4708 4742 1903
Size: 34 50 34 311 34 2342 34 3678
Fragments arranged by size:
3678 2342 311 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2365 2395 3371 3509 4409 5619 2365
Size: 30 976 138 900 1210 3263
Fragments arranged by size:
3263 1210 976 900 138 30
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6517
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2840 3494 5128
Size: 107 122 71 631 605 1168 654 1634
Cuts at: 5128 136
Size: 1525
Fragments arranged by size:
1634 1525 1168 654 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6512 6512
Size: 6517
BlpI GC'TnA_GC
Cuts at: 3934 3934
Size: 6517
Bme1580I G_kGCm'C
Cuts at: 1790 2671 4578 5734 1790
Size: 881 1907 1156 2573
Fragments arranged by size:
2573 1907 1156 881
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2480 3065 3329
Size: 416 53 83 399 24 1063 585 264
Cuts at: 3329 3521 3791 4248 5206 442
Size: 192 270 457 958 1753
Fragments arranged by size:
1753 1063 958 585 457 416 399 270
264 192 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5177 1239
Size: 3938 2579
Bpu10I CC'TnA_GC
Cuts at: 2802 2802
Size: 6517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4642 5507 5805 4642
Size: 865 298 5354
Fragments arranged by size:
5354 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2901 5180 882
Size: 617 1402 2279 2219
Fragments arranged by size:
2279 2219 1402 617
BsaAI yAC'GTr
Cuts at: 493 1204 2109 4308 493
Size: 711 905 2199 2702
Fragments arranged by size:
2702 2199 905 711
BsaBI GATnn'nnATC
Cuts at: 6511 6511
Size: 6517
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4324 4706 5341 275
Size: 53 83 186 936 2791 382 635 1451
Fragments arranged by size:
2791 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4950 5348 5622 5769 6180 4950
Size: 398 274 147 411 5287
Fragments arranged by size:
5287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2691 2721 1178
Size: 30 165 30 1288 30 4974
Fragments arranged by size:
4974 1288 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6517
BseYI C'CCAG_C
Cuts at: 2008 2165 2499 3048 3484 3615 5720 6216 2008
Size: 157 334 549 436 131 2105 496 2309
Fragments arranged by size:
2309 2105 549 496 436 334 157 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 2491 2849 2967 1163
Size: 573 344 411 358 118 4713
Fragments arranged by size:
4713 573 411 358 344 118
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3967 4728 5756 1540
Size: 275 186 1966 761 1028 2301
Fragments arranged by size:
2301 1966 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4578 4663 5734 4578
Size: 85 1071 5361
Fragments arranged by size:
5361 1071 85
BsmI GAATG_Cn'
Cuts at: 4028 4121 4028
Size: 93 6424
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2671 2808 3836 4578 4663 5734
Size: 399 606 275 137 1028 742 85 1071
Cuts at: 5734 1391
Size: 2174
Fragments arranged by size:
2174 1071 1028 742 606 399 275 137
85
BspEI T'CCGG_A
Cuts at: 6180 6180
Size: 6517
BspHI T'CATG_A
Cuts at: 2124 4406 6136 2124
Size: 2282 1730 2505
Fragments arranged by size:
2505 2282 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2180 2989 3872 844
Size: 1336 809 883 3489
Fragments arranged by size:
3489 1336 883 809
BsrBI CCG'CTC
Cuts at: 1394 1823 2742 4404 1394
Size: 429 919 1662 3507
Fragments arranged by size:
3507 1662 919 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5012 5186 65
Size: 1183 3764 174 1396
Fragments arranged by size:
3764 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5161 5348 1700
Size: 110 3351 187 2869
Fragments arranged by size:
3351 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6517
BssSI C'ACGA_G
Cuts at: 2670 4577 5589 2670
Size: 1907 1012 3598
Fragments arranged by size:
3598 1907 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2465 794
Size: 1671 4846
BstBI TT'CG_AA
Cuts at: 5321 5321
Size: 6517
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6517
BstXI CCAn_nnnn'nTGG
Cuts at: 3234 3300 3492 3234
Size: 66 192 6259
Fragments arranged by size:
6259 192 66
BstYI r'GATC_y
Cuts at: 1972 2271 2308 3261 3847 4214 4599 4616 6068
Size: 299 37 953 586 367 385 17 1452
Cuts at: 6068 6154 6166 6512 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 953 586 385 367 346 299
86 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 3373 3373
Size: 6517
BtgI C'CryG_G
Cuts at: 513 1065 2388 2421 2918 513
Size: 552 1323 33 497 4112
Fragments arranged by size:
4112 1323 552 497 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5778
Fragments arranged by size:
5778 683 56
BtsI GCAGTG_nn'
Cuts at: 2092 4033 4827 4847 2092
Size: 1941 794 20 3762
Fragments arranged by size:
3762 1941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6482
DraI TTT'AAA
Cuts at: 2652 3198 3821 2652
Size: 546 623 5348
Fragments arranged by size:
5348 623 546
DraIII CAC_nnn'GTG
Cuts at: 2109 2514 2109
Size: 405 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5524 809
Size: 507 4208 1802
Fragments arranged by size:
4208 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2817 2915 3964
Size: 54 1425 50 275 18 987 98 1049
Cuts at: 3964 4853 6482 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1049 987 889 275 98 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 1537
Size: 275 2152 4090
Fragments arranged by size:
4090 2152 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2974 3681 4447 1353
Size: 1621 707 766 3423
Fragments arranged by size:
3423 1621 766 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5104 5478 5624 387
Size: 1064 138 3515 374 146 1280
Fragments arranged by size:
3515 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2398 4580 5177 5964 6232 1239
Size: 393 766 2182 597 787 268 1524
Fragments arranged by size:
2182 1524 787 766 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3359 3680 3359
Size: 321 6196
EcoO109I rG'GnC_Cy
Cuts at: 2169 3309 3597 2169
Size: 1140 288 5089
Fragments arranged by size:
5089 1140 288
EcoRI G'AATT_C
Cuts at: 3826 4204 3826
Size: 378 6139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2327 2327
Size: 6517
FspI TGC'GCA
Cuts at: 5023 5023
Size: 6517
HaeII r_GCGC'y
Cuts at: 1678 5664 1678
Size: 3986 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2339 2371 2691
Size: 32 135 32 671 32 64 32 320
Cuts at: 2691 2723 3369 3401 5158 5190 5232 5264 1373
Size: 32 646 32 1757 32 42 32 2626
Fragments arranged by size:
2626 1757 671 646 320 135 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2788 3084 3861 4107 669
Size: 988 281 850 296 777 246 3079
Fragments arranged by size:
3079 988 850 777 296 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6517
HpaI GTT'AAC
Cuts at: 2788 3084 4107 2788
Size: 296 1023 5198
Fragments arranged by size:
5198 1023 296
KpnI G_GTAC'C
Cuts at: 3214 3842 3214
Size: 628 5889
MfeI C'AATT_G
Cuts at: 4116 4116
Size: 6517
MluI A'CGCG_T
Cuts at: 6393 6393
Size: 6517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5631 5815 1305
Size: 4326 184 2007
Fragments arranged by size:
4326 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2819 2917 10
Size: 54 1425 343 987 98 3610
Fragments arranged by size:
3610 1425 987 343 98 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3629 4475 4834 4993 518
Size: 648 2463 846 359 159 2042
Fragments arranged by size:
2463 2042 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2679 3933 4610 5758 6003 1606
Size: 1073 1254 677 1148 245 2120
Fragments arranged by size:
2120 1254 1148 1073 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2388 2421 2918 513
Size: 552 1323 33 497 4112
Fragments arranged by size:
4112 1323 552 497 33
NdeI CA'TA_TG
Cuts at: 387 2636 387
Size: 2249 4268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6517
NotI GC'GGCC_GC
Cuts at: 3964 3964
Size: 6517
NspI r_CATG'y
Cuts at: 1132 1229 2023 2786 3538 3875 5420 1132
Size: 97 794 763 752 337 1545 2229
Fragments arranged by size:
2229 1545 794 763 752 337 97
PasI CC'CwG_GG
Cuts at: 1348 2076 1348
Size: 728 5789
PciI A'CATG_T
Cuts at: 5416 5416
Size: 6517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6517
PmeI GTTT'AAAC
Cuts at: 3821 3821
Size: 6517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2205 4586 4618 6099 6131 2173
Size: 32 2381 32 1481 32 2559
Fragments arranged by size:
2559 2381 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2169 2169
Size: 6517
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6517
PsiI TTA'TAA
Cuts at: 2580 3551 4087 2580
Size: 971 536 5010
Fragments arranged by size:
5010 971 536
PstI C_TGCA'G
Cuts at: 830 3595 3869 830
Size: 2765 274 3478
Fragments arranged by size:
3478 2765 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6517
SalI G'TCGA_C
Cuts at: 1936 3859 1936
Size: 1923 4594
SbfI CC_TGCA'GG
Cuts at: 3869 3869
Size: 6517
ScaI AGT'ACT
Cuts at: 2429 3567 4765 6477 2429
Size: 1138 1198 1712 2469
Fragments arranged by size:
2469 1712 1198 1138
SfcI C'TryA_G
Cuts at: 826 1045 2431 3426 3591 3865 5000 5681 5872
Size: 219 1386 995 165 274 1135 681 191
Cuts at: 5872 826
Size: 1471
Fragments arranged by size:
1471 1386 1135 995 681 274 219 191
165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6517
SmaI CCC'GGG
Cuts at: 1872 3844 1872
Size: 1972 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4621 5522 5784 820
Size: 197 926 2678 901 262 1553
Fragments arranged by size:
2678 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4308 493
Size: 3815 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6517
SphI G_CATG'C
Cuts at: 1132 3875 1132
Size: 2743 3774
SspI AAT'ATT
Cuts at: 5 52 3104 3476 4441 5
Size: 47 3052 372 965 2081
Fragments arranged by size:
3052 2081 965 372 47
StuI AGG'CCT
Cuts at: 1517 3059 1517
Size: 1542 4975
StyI C'CwwG_G
Cuts at: 513 1065 2176 2325 2388 2421 2814 2918 3091
Size: 552 1111 149 63 33 393 104 173
Cuts at: 3091 3956 513
Size: 865 3074
Fragments arranged by size:
3074 1111 865 552 393 173 149 104
63 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 3183 4544 4561 4714 4899 5344
Size: 1255 534 1069 1361 17 153 185 445
Cuts at: 5344 325
Size: 1498
Fragments arranged by size:
1498 1361 1255 1069 534 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2186 2427 3316
Size: 274 80 33 51 1443 209 241 889
Cuts at: 3316 3565 4763 6475 96
Size: 249 1198 1712 138
Fragments arranged by size:
1712 1443 1198 889 274 249 241 209
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3430 3462 6477 6509 3430
Size: 32 3015 32 3438
Fragments arranged by size:
3438 3015 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6517
XbaI T'CTAG_A
Cuts at: 3853 3853
Size: 6517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3804 3804
Size: 6517
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6517
XmaI C'CCGG_G
Cuts at: 1870 3842 1870
Size: 1972 4545
XmnI GAAnn'nnTTC
Cuts at: 4646 4646
Size: 6517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4325 276
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI