Restriction map for
pFN21AE1259
(Circular) (Six-base) MAPSORT of: pFN21AE1259.seq Check: 9458 from: 1 to: 6484
pFN21AE1259 6484 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2796 4294 278
Size: 53 83 186 936 1260 1498 2468
Fragments arranged by size:
2468 1498 1260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3325 3805 3325
Size: 480 6004
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2296 2336 4547 5931 6199 1632
Size: 476 188 40 2211 1384 268 1917
Fragments arranged by size:
2211 1917 1384 476 268 188 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 2093 3252 5383 6360 1203
Size: 890 1159 2131 977 1327
Fragments arranged by size:
2131 1327 1159 977 890
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 3669 5213 3669
Size: 1544 4940
AleI CACnn'nnGTG
Cuts at: 1166 2457 1166
Size: 1291 5193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2442 1184
Size: 1258 5226
AlwNI CAG_nnn'CTG
Cuts at: 2251 2623 3667 4227 5799 2251
Size: 372 1044 560 1572 2936
Fragments arranged by size:
2936 1572 1044 560 372
ApaI G_GGCC'C
Cuts at: 2350 2350
Size: 6484
ApaLI G'TGCA_C
Cuts at: 2912 4541 5697 2912
Size: 1629 1156 3699
Fragments arranged by size:
3699 1629 1156
ApoI r'AATT_y
Cuts at: 1493 3129 3793 4019 4171 6280 1493
Size: 1636 664 226 152 2109 1697
Fragments arranged by size:
2109 1697 1636 664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3182 3470 3809 1870
Size: 73 1239 288 339 4545
Fragments arranged by size:
4545 1239 339 288 73
BamHI G'GATC_C
Cuts at: 3171 3814 4181 3171
Size: 643 367 5474
Fragments arranged by size:
5474 643 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2451 3289 3325 3411 3638
Size: 325 217 627 664 838 36 86 227
Cuts at: 3638 3776 3805 5260 618
Size: 138 29 1455 1842
Fragments arranged by size:
1842 1455 838 664 627 325 227 217
138 86 36 29
BanII G_rGCy'C
Cuts at: 1391 2350 3237 3609 3803 1391
Size: 959 887 372 194 4072
Fragments arranged by size:
4072 959 887 372 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2096 3033 928
Size: 971 197 937 4379
Fragments arranged by size:
4379 971 937 197
BbvCI CC'TCA_GC
Cuts at: 3345 3345
Size: 6484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2818 2852 3486 3520 4675
Size: 34 50 34 797 34 634 34 1155
Cuts at: 4675 4709 1903
Size: 34 3678
Fragments arranged by size:
3678 1155 797 634 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2333 2783 4376 5586 2333
Size: 450 1593 1210 3231
Fragments arranged by size:
3231 1593 1210 450
BclI T'GATC_A
Cuts at: 1548 2139 1548
Size: 591 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2594 5095 136
Size: 107 122 71 631 605 922 2501 1525
Fragments arranged by size:
2501 1525 922 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 2350 2916 3183 3294 3681 4545 5701 1790
Size: 560 566 267 111 387 864 1156 2573
Fragments arranged by size:
2573 1156 864 566 560 387 267 111
BmgBI CAC'GTC
Cuts at: 1318 2094 3253 1318
Size: 776 1159 4549
Fragments arranged by size:
4549 1159 776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2172 2611 4215
Size: 416 53 83 399 24 755 439 1604
Cuts at: 4215 5173 442
Size: 958 1753
Fragments arranged by size:
1753 1604 958 755 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2259 3370 2259
Size: 1111 5373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2837 3547 5144 1239
Size: 1598 710 1597 2579
Fragments arranged by size:
2579 1598 1597 710
Bpu10I CC'TnA_GC
Cuts at: 2368 3345 2368
Size: 977 5507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4609 5474 5772 4609
Size: 865 298 5321
Fragments arranged by size:
5321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5147 882
Size: 617 3648 2219
Fragments arranged by size:
3648 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3057 4275 493
Size: 711 1853 1218 2702
Fragments arranged by size:
2702 1853 1218 711
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2328 2583 2793 3501
Size: 53 83 186 936 795 255 210 708
Cuts at: 3501 4291 4673 5308 275
Size: 790 382 635 1451
Fragments arranged by size:
1451 936 795 790 708 635 382 255
210 186 83 53
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6259
Fragments arranged by size:
6259 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2731 2879 2999 1186
Size: 1545 148 120 4671
Fragments arranged by size:
4671 1545 148 120
BseYI C'CCAG_C
Cuts at: 2629 3681 5687 6183 2629
Size: 1052 2006 496 2930
Fragments arranged by size:
2930 2006 1052 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2254 1163
Size: 573 518 5393
Fragments arranged by size:
5393 573 518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2259 3216 3220 3496 3934 4695
Size: 275 186 258 957 4 276 438 761
Cuts at: 4695 5723 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 957 761 438 276 275 258
186 4
BsiHKAI G_wGCw'C
Cuts at: 2916 3210 4545 4630 5701 2916
Size: 294 1335 85 1071 3699
Fragments arranged by size:
3699 1335 1071 294 85
BsmI GAATG_Cn'
Cuts at: 2288 2939 3995 4088 2288
Size: 651 1056 93 4684
Fragments arranged by size:
4684 1056 651 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2350 2916 3183 3210 3237 3294 3609
Size: 399 560 566 267 27 27 57 315
Cuts at: 3609 3681 3803 4545 4630 5701 1391
Size: 72 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 566 560 399 315 267
122 85 72 57 27 27
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 3839 844
Size: 1825 1170 3489
Fragments arranged by size:
3489 1825 1170
BsrBI CCG'CTC
Cuts at: 1394 1823 2266 3479 4371 1394
Size: 429 443 1213 892 3507
Fragments arranged by size:
3507 1213 892 443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2341 4979 5153 65
Size: 1183 1093 2638 174 1396
Fragments arranged by size:
2638 1396 1183 1093 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2432 2651 2696 2705 3215 5128
Size: 110 249 373 219 45 9 510 1913
Cuts at: 5128 5315 1700
Size: 187 2869
Fragments arranged by size:
2869 1913 510 373 249 219 187 110
45 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 2932 3238 3761 4544 5556 2932
Size: 306 523 783 1012 3860
Fragments arranged by size:
3860 1012 783 523 306
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 2815 3767 2815
Size: 952 5532
BstYI r'GATC_y
Cuts at: 1972 2167 3171 3186 3814 4181 4566 4583 6035
Size: 195 1004 15 628 367 385 17 1452
Cuts at: 6035 6121 6133 6479 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1004 628 385 367 346 195
86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2591 2591
Size: 6484
BtgI C'CryG_G
Cuts at: 513 1065 2476 2506 2808 3073 3642 3716 513
Size: 552 1411 30 302 265 569 74 3281
Fragments arranged by size:
3281 1411 569 552 302 265 74 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 2485 4000 4794 4814 2485
Size: 1515 794 20 4155
Fragments arranged by size:
4155 1515 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 2122 3039 3788 2122
Size: 917 749 4818
Fragments arranged by size:
4818 917 749
DraIII CAC_nnn'GTG
Cuts at: 3622 3622
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2653 2703
Size: 54 1425 50 275 18 426 397 50
Cuts at: 2703 3197 3213 3217 3313 3493 3931 4820 6449
Size: 494 16 4 96 180 438 889 1629
Cuts at: 6449 8
Size: 43
Fragments arranged by size:
1629 1425 889 494 438 426 397 275
180 96 54 50 50 43 18 16
4
EagI C'GGCC_G
Cuts at: 1537 1812 2256 3213 3217 3493 3931 1537
Size: 275 444 957 4 276 438 4090
Fragments arranged by size:
4090 957 444 438 276 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2897 4414 1353
Size: 1544 1517 3423
Fragments arranged by size:
3423 1544 1517
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2002 2162 5071 5445 5591 387
Size: 1064 138 413 160 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 413 374 160 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2296 2336 2837 3547 4547 5144
Size: 393 476 188 40 501 710 1000 597
Cuts at: 5144 5931 6199 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1000 787 710 597 501 476 393
268 188 40
EcoO109I rG'GnC_Cy
Cuts at: 2347 2568 3483 3568 2347
Size: 221 915 85 5263
Fragments arranged by size:
5263 915 221 85
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FseI GG_CCGG'CC
Cuts at: 3219 3219
Size: 6484
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 2630 5631 1678
Size: 952 3001 2531
Fragments arranged by size:
3001 2531 952
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3655 3687 5125 5157 5199
Size: 32 135 32 2083 32 1438 32 42
Cuts at: 5199 5231 1373
Size: 32 2626
Fragments arranged by size:
2626 2083 1438 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3329 3809 3329
Size: 480 6004
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3630 5598 5782 1305
Size: 2325 1968 184 2007
Fragments arranged by size:
2325 2007 1968 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3315 10
Size: 54 1425 343 1483 3179
Fragments arranged by size:
3179 1483 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2424 2457 2699 2874 3381 3765 4442
Size: 648 1258 33 242 175 507 384 677
Cuts at: 4442 4801 4960 518
Size: 359 159 2042
Fragments arranged by size:
2042 1258 677 648 507 384 359 242
175 159 33
MspA1I CmG'CkG
Cuts at: 1606 2478 3274 3644 3900 4577 5725 5970 1606
Size: 872 796 370 256 677 1148 245 2120
Fragments arranged by size:
2120 1148 872 796 677 370 256 245
NaeI GCC'GGC
Cuts at: 2653 2707 3217 2653
Size: 54 510 5920
Fragments arranged by size:
5920 510 54
NcoI C'CATG_G
Cuts at: 513 1065 2808 3073 513
Size: 552 1743 265 3924
Fragments arranged by size:
3924 1743 552 265
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NgoMIV G'CCGG_C
Cuts at: 2651 2705 3215 2651
Size: 54 510 5920
Fragments arranged by size:
5920 510 54
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 2256 3931 2256
Size: 1675 4809
NspI r_CATG'y
Cuts at: 1132 1229 2533 2912 3842 5387 1132
Size: 97 1304 379 930 1545 2229
Fragments arranged by size:
2229 1545 1304 930 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 3277 3322 1108
Size: 1515 654 45 4270
Fragments arranged by size:
4270 1515 654 45
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2351 2383 4553 4585 6066 6098 2351
Size: 32 2170 32 1481 32 2737
Fragments arranged by size:
2737 2170 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3568 3568
Size: 6484
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2346 2346
Size: 6484
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 6452
PstI C_TGCA'G
Cuts at: 830 3339 3726 3836 830
Size: 2509 387 110 3478
Fragments arranged by size:
3478 2509 387 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 3274 3274
Size: 6484
SacII CC_GC'GG
Cuts at: 2479 3645 2479
Size: 1166 5318
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SanDI GG'GwC_CC
Cuts at: 3568 3568
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 3335 3722 3832 4967 5648 5839 826
Size: 219 2290 387 110 1135 681 191 1471
Fragments arranged by size:
2290 1471 1135 681 387 219 191 110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3184 3811 1872
Size: 1312 627 4545
Fragments arranged by size:
4545 1312 627
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4588 5489 5751 820
Size: 197 926 2645 901 262 1553
Fragments arranged by size:
2645 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 2533 3842 1132
Size: 1401 1309 3774
Fragments arranged by size:
3774 1401 1309
SspI AAT'ATT
Cuts at: 5 52 2209 4408 5
Size: 47 2157 2199 2081
Fragments arranged by size:
2199 2157 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6484
StyI C'CwwG_G
Cuts at: 513 1065 2808 3073 3923 513
Size: 552 1743 265 850 3074
Fragments arranged by size:
3074 1743 850 552 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2630 3269 4511 4528 4681 4866 5311
Size: 1255 1050 639 1242 17 153 185 445
Cuts at: 5311 325
Size: 1498
Fragments arranged by size:
1498 1255 1242 1050 639 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4730 6442 96
Size: 274 80 33 51 1443 2753 1712 138
Fragments arranged by size:
2753 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3351 3383 6444 6476 3351
Size: 32 3061 32 3359
Fragments arranged by size:
3359 3061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3244 3278 3422 3244
Size: 34 144 6306
Fragments arranged by size:
6306 144 34
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3182 3809 1870
Size: 1312 627 4545
Fragments arranged by size:
4545 1312 627
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2794 4292 276
Size: 53 83 186 936 1260 1498 2468
Fragments arranged by size:
2468 1498 1260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI