Restriction map for
pFN21AE1254
(Circular) (Six-base) MAPSORT of: pFN21AE1254.seq Check: 5669 from: 1 to: 6346
pFN21AE1254 6346 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4156 278
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 2354 3689 1937
Size: 72 345 1335 4594
Fragments arranged by size:
4594 1335 345 72
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 3079 4473 4846 1563
Size: 1516 1394 373 3063
Fragments arranged by size:
3063 1516 1394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2724 2990 4409 5793 6061 1632
Size: 482 610 266 1419 1384 268 1917
Fragments arranged by size:
1917 1419 1384 610 482 268 266
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6149
AflIII A'CryG_T
Cuts at: 1203 5245 6222 1203
Size: 4042 977 1327
Fragments arranged by size:
4042 1327 977
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 2739 5075 2739
Size: 2336 4010
AleI CACnn'nnGTG
Cuts at: 1166 2580 2726 1166
Size: 1414 146 4786
Fragments arranged by size:
4786 1414 146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6346
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 2543
Size: 32 6314
AlwNI CAG_nnn'CTG
Cuts at: 4089 5661 4089
Size: 1572 4774
ApaLI G'TGCA_C
Cuts at: 4403 5559 4403
Size: 1156 5190
ApoI r'AATT_y
Cuts at: 1493 2524 3496 3655 3881 4033 6142 1493
Size: 1031 972 159 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 972 226 159 152
AseI AT'TA_AT
Cuts at: 160 1032 2445 4900 160
Size: 872 1413 2455 1606
Fragments arranged by size:
2455 1606 1413 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 3671 1870
Size: 73 1728 4545
Fragments arranged by size:
4545 1728 73
BamHI G'GATC_C
Cuts at: 2856 3676 4043 2856
Size: 820 367 5159
Fragments arranged by size:
5159 820 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3667 5122 618
Size: 325 217 627 1880 1455 1842
Fragments arranged by size:
1880 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2878 3665 1391
Size: 1487 787 4072
Fragments arranged by size:
4072 1487 787
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2124 2292 2406 2738 3537 928
Size: 971 225 168 114 332 799 3737
Fragments arranged by size:
3737 971 799 332 225 168 114
BbvCI CC'TCA_GC
Cuts at: 2990 2990
Size: 6346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2750 2784 4537 4571 1903
Size: 34 813 34 1753 34 3678
Fragments arranged by size:
3678 1753 813 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2993 3101 4238 5448 2993
Size: 108 1137 1210 3891
Fragments arranged by size:
3891 1210 1137 108
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3416 4957 136
Size: 107 122 71 631 605 1744 1541 1525
Fragments arranged by size:
1744 1541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 3763 3763
Size: 6346
Bme1580I G_kGCm'C
Cuts at: 1790 3412 4407 5563 1790
Size: 1622 995 1156 2573
Fragments arranged by size:
2573 1622 1156 995
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4077 5035 442
Size: 416 53 83 399 24 2660 958 1753
Fragments arranged by size:
2660 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3261 3582 3261
Size: 321 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 5006 1239
Size: 1625 2142 2579
Fragments arranged by size:
2579 2142 1625
Bpu10I CC'TnA_GC
Cuts at: 2153 2990 2153
Size: 837 5509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2696 4471 5336 5634 2696
Size: 1775 865 298 3408
Fragments arranged by size:
3408 1775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4137 493
Size: 711 2933 2702
Fragments arranged by size:
2933 2702 711
BsaBI GATnn'nnATC
Cuts at: 6340 6340
Size: 6346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4153 4535 5170 275
Size: 53 83 186 936 2620 382 635 1451
Fragments arranged by size:
2620 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4779 5177 5451 5598 6009 4779
Size: 398 274 147 411 5116
Fragments arranged by size:
5116 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2360 2390 3253 3283 1178
Size: 30 165 30 957 30 863 30 4241
Fragments arranged by size:
4241 957 863 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2102 2375 2378 2381 2384 2387 2405
Size: 871 45 273 3 3 3 3 18
Cuts at: 2405 3250 3258 3261 3264 3273 3276 3279 3282
Size: 845 8 3 3 9 3 3 3
Cuts at: 3282 3318 3321 3324 3333 1186
Size: 36 3 3 9 4199
Fragments arranged by size:
4199 871 845 273 45 36 18 9
9 8 3 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2260 2563 2995 5549 6045 2260
Size: 303 432 2554 496 2561
Fragments arranged by size:
2561 2554 496 432 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2790 1163
Size: 573 1054 4719
Fragments arranged by size:
4719 1054 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3796 4557 5585 1540
Size: 275 186 1795 761 1028 2301
Fragments arranged by size:
2301 1795 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 2878 2898 4407 4492 5563 2811
Size: 67 20 1509 85 1071 3594
Fragments arranged by size:
3594 1509 1071 85 67 20
BsmI GAATG_Cn'
Cuts at: 3857 3950 3857
Size: 93 6253
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2811 2878 2898 3412 3665 4407 4492
Size: 399 1021 67 20 514 253 742 85
Cuts at: 4492 5563 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1021 742 514 399 253 85
67 20
BspEI T'CCGG_A
Cuts at: 6009 6009
Size: 6346
BspHI T'CATG_A
Cuts at: 2868 3568 4235 5965 2868
Size: 700 667 1730 3249
Fragments arranged by size:
3249 1730 700 667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3701 844
Size: 2857 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4233 1394
Size: 429 2410 3507
Fragments arranged by size:
3507 2410 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2548 2635 2781 3378 4841 5015 65
Size: 1183 1300 87 146 597 1463 174 1396
Fragments arranged by size:
1463 1396 1300 1183 597 174 146 87
BsrFI r'CCGG_y
Cuts at: 1700 1810 4990 5177 1700
Size: 110 3180 187 2869
Fragments arranged by size:
3180 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6346
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 4406 5418 4406
Size: 1012 5334
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6346
BstBI TT'CG_AA
Cuts at: 5150 5150
Size: 6346
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6346
BstYI r'GATC_y
Cuts at: 1972 2856 3676 4043 4428 4445 5897 5983 5995
Size: 884 820 367 385 17 1452 86 12
Cuts at: 5995 6341 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 884 820 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2355 2355
Size: 6346
BtgI C'CryG_G
Cuts at: 513 1065 2145 2775 513
Size: 552 1080 630 4084
Fragments arranged by size:
4084 1080 630 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 3862 4656 4676 3862
Size: 794 20 5532
Fragments arranged by size:
5532 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 2503 3650 2503
Size: 1147 5199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5353 809
Size: 507 4037 1802
Fragments arranged by size:
4037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2745 2778 2862
Size: 54 1425 50 275 18 915 33 84
Cuts at: 2862 3258 3793 4682 6311 8
Size: 396 535 889 1629 43
Fragments arranged by size:
1629 1425 915 889 535 396 275 84
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3793 1537
Size: 275 1981 4090
Fragments arranged by size:
4090 1981 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2020 2301 2382 2406 3169 4276 1353
Size: 667 281 81 24 763 1107 3423
Fragments arranged by size:
3423 1107 763 667 281 81 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4933 5307 5453 387
Size: 1064 138 3344 374 146 1280
Fragments arranged by size:
3344 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2876 2876
Size: 6346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2724 2864 2990 4409 5006 5793
Size: 393 482 610 140 126 1419 597 787
Cuts at: 5793 6061 1239
Size: 268 1524
Fragments arranged by size:
1524 1419 787 610 597 482 393 268
140 126
EcoO109I rG'GnC_Cy
Cuts at: 3119 3119
Size: 6346
EcoRI G'AATT_C
Cuts at: 3655 4033 3655
Size: 378 5968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2327 2327
Size: 6346
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 5493 1678
Size: 3815 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 2725 2757 3226
Size: 32 135 32 788 32 333 32 469
Cuts at: 3226 3258 4987 5019 5061 5093 1373
Size: 32 1729 32 42 32 2626
Fragments arranged by size:
2626 1729 788 469 333 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3690 3936 669
Size: 988 281 1752 246 3079
Fragments arranged by size:
3079 1752 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6346
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 2424 3945 2424
Size: 1521 4825
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2859 5460 5644 1305
Size: 789 765 2601 184 2007
Fragments arranged by size:
2601 2007 789 765 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 2780 2864 3260 10
Size: 54 1425 343 915 33 84 396 3096
Fragments arranged by size:
3096 1425 915 396 343 84 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 2726 4304 4663 4822 518
Size: 648 1414 146 1578 359 159 2042
Fragments arranged by size:
2042 1578 1414 648 359 159 146
MspA1I CmG'CkG
Cuts at: 1606 2567 3762 4439 5587 5832 1606
Size: 961 1195 677 1148 245 2120
Fragments arranged by size:
2120 1195 1148 961 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2775 513
Size: 552 1710 4084
Fragments arranged by size:
4084 1710 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6346
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 2759 3704 5249 1132
Size: 97 1530 945 1545 2229
Fragments arranged by size:
2229 1545 1530 945 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 5245 5245
Size: 6346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3141 1108
Size: 2033 4313
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3119 3119
Size: 6346
PshAI GACnn'nnGTC
Cuts at: 1613 3137 1613
Size: 1524 4822
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PstI C_TGCA'G
Cuts at: 830 3698 830
Size: 2868 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 2567 2567
Size: 6346
SacI G_AGCT'C
Cuts at: 2878 2878
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SapI GCTCTTCn'nnn_
Cuts at: 2301 3169 2301
Size: 868 5478
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 4594 6306 4594
Size: 1712 4634
SfcI C'TryA_G
Cuts at: 826 1045 2592 3694 4829 5510 5701 826
Size: 219 1547 1102 1135 681 191 1471
Fragments arranged by size:
1547 1471 1135 1102 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 3673 1872
Size: 1801 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2711 4450 5351 5613 820
Size: 197 926 768 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 768 262 197
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 2759 3704 1132
Size: 1627 945 3774
Fragments arranged by size:
3774 1627 945
SspI AAT'ATT
Cuts at: 5 52 4270 5
Size: 47 4218 2081
Fragments arranged by size:
4218 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6346
StyI C'CwwG_G
Cuts at: 513 1065 2775 3785 513
Size: 552 1710 1010 3074
Fragments arranged by size:
3074 1710 1010 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4373 4390 4543 4728 5173 325
Size: 1255 2793 17 153 185 445 1498
Fragments arranged by size:
2793 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4592 6304 96
Size: 274 80 33 51 1443 2615 1712 138
Fragments arranged by size:
2615 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3253 3285 6306 6338 3253
Size: 32 3021 32 3261
Fragments arranged by size:
3261 3021 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6346
XbaI T'CTAG_A
Cuts at: 3682 3682
Size: 6346
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6346
XmaI C'CCGG_G
Cuts at: 1870 3671 1870
Size: 1801 4545
XmnI GAAnn'nnTTC
Cuts at: 2735 3588 4475 2735
Size: 853 887 4606
Fragments arranged by size:
4606 887 853
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4154 276
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI