Restriction map for
pFN21AE1249
(Circular) (Six-base) MAPSORT of: pFN21AE1249.seq Check: 1856 from: 1 to: 6187
pFN21AE1249 6187 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2352 2352
Size: 6187
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3026 4250 5634 5902 1632
Size: 1394 1224 1384 268 1917
Fragments arranged by size:
1917 1394 1384 1224 268
AfeI AGC'GCT
Cuts at: 3011 3011
Size: 6187
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2594 1184
Size: 1410 4777
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 6155
AlwNI CAG_nnn'CTG
Cuts at: 2344 2482 3259 3930 5502 2344
Size: 138 777 671 1572 3029
Fragments arranged by size:
3029 1572 777 671 138
ApaI G_GGCC'C
Cuts at: 2264 3455 2264
Size: 1191 4996
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 3496 3722 3874 5983 1493
Size: 2003 226 152 2109 1697
Fragments arranged by size:
2109 2003 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2126 2134 3446 3512 1870
Size: 73 134 49 8 1312 66 4545
Fragments arranged by size:
4545 1312 134 73 66 49 8
BamHI G'GATC_C
Cuts at: 3045 3517 3884 3045
Size: 472 367 5348
Fragments arranged by size:
5348 472 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2130 2195 2296 2312
Size: 325 217 627 335 8 65 101 16
Cuts at: 2312 2639 2857 3402 3461 3508 4963 618
Size: 327 218 545 59 47 1455 1842
Fragments arranged by size:
1842 1455 627 545 335 327 325 218
217 101 65 59 47 16 8
BanII G_rGCy'C
Cuts at: 1391 2078 2264 3369 3435 3455 3506 1391
Size: 687 186 1105 66 20 51 4072
Fragments arranged by size:
4072 1105 687 186 66 51 20
BbeI G_GCGC'C
Cuts at: 2126 2134 2199 2300 2643 2861 3406 3465 2126
Size: 8 65 101 343 218 545 59 4848
Fragments arranged by size:
4848 545 343 218 101 65 59 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5216
BbvCI CC'TCA_GC
Cuts at: 3426 3426
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3274 3308 4378 4412 1903
Size: 34 1337 34 1070 34 3678
Fragments arranged by size:
3678 1337 1070 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2031 2153 2294
Size: 107 122 71 631 605 359 122 141
Cuts at: 2294 2310 2697 3459 4798 136
Size: 16 387 762 1339 1525
Fragments arranged by size:
1525 1339 762 631 605 387 359 141
122 122 107 71 16
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 2012 2072 3604 2012
Size: 60 1532 4595
Fragments arranged by size:
4595 1532 60
Bme1580I G_kGCm'C
Cuts at: 1790 2264 3068 3455 4248 5404 1790
Size: 474 804 387 793 1156 2573
Fragments arranged by size:
2573 1156 804 793 474 387
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 4847 1239
Size: 800 2808 2579
Fragments arranged by size:
2808 2579 800
Bpu10I CC'TnA_GC
Cuts at: 3426 3426
Size: 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3119 4850 882
Size: 617 1620 1731 2219
Fragments arranged by size:
2219 1731 1620 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 3296 6181 3296
Size: 2885 3302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 2131 2196 2297
Size: 53 83 186 936 590 8 65 101
Cuts at: 2297 2640 2858 3403 3462 3994 4376 5011 275
Size: 343 218 545 59 532 382 635 1451
Fragments arranged by size:
1451 936 635 590 545 532 382 343
218 186 101 83 65 59 53 8
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3149 3179 1178
Size: 30 165 30 1746 30 4186
Fragments arranged by size:
4186 1746 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2975 3443 1186
Size: 1789 468 3930
Fragments arranged by size:
3930 1789 468
BseYI C'CCAG_C
Cuts at: 2708 2758 2863 2939 3420 3434 5390 5886 2708
Size: 50 105 76 481 14 1956 496 3009
Fragments arranged by size:
3009 1956 496 481 105 76 50 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2021 2252 2618 1163
Size: 573 285 231 366 4732
Fragments arranged by size:
4732 573 366 285 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2361 2394 2400 2451 2883 2958
Size: 275 186 360 33 6 51 432 75
Cuts at: 2958 3121 3320 3637 4398 5426 1540
Size: 163 199 317 761 1028 2301
Fragments arranged by size:
2301 1028 761 432 360 317 275 199
186 163 75 51 33 6
BsiHKAI G_wGCw'C
Cuts at: 3369 4248 4333 5404 3369
Size: 879 85 1071 4152
Fragments arranged by size:
4152 1071 879 85
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2264 3068 3369 3435 3455 3506
Size: 399 288 186 804 301 66 20 51
Cuts at: 3506 4248 4333 5404 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 804 742 399 301 288 186
85 66 51 20
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 3390 4076 5806 3390
Size: 686 1730 3771
Fragments arranged by size:
3771 1730 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 3087 3282 3325 3542 844
Size: 1508 735 195 43 217 3489
Fragments arranged by size:
3489 1508 735 217 195 43
BsrBI CCG'CTC
Cuts at: 1394 1823 2353 2499 2527 2828 2928 4074 1394
Size: 429 530 146 28 301 100 1146 3507
Fragments arranged by size:
3507 1146 530 429 301 146 100 28
BsrDI GCAATG_nn'
Cuts at: 65 1248 2283 4682 4856 65
Size: 1183 1035 2399 174 1396
Fragments arranged by size:
2399 1396 1183 1035 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 3458 4831 5018 1700
Size: 110 337 1311 1373 187 2869
Fragments arranged by size:
2869 1373 1311 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 2087 3232 1925
Size: 162 1145 4880
Fragments arranged by size:
4880 1145 162
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2289 2344 794
Size: 1495 55 4637
Fragments arranged by size:
4637 1495 55
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 3337 1184
Size: 2153 4034
BstXI CCAn_nnnn'nTGG
Cuts at: 2708 2980 2708
Size: 272 5915
BstYI r'GATC_y
Cuts at: 1972 3045 3517 3884 4269 4286 5738 5824 5836
Size: 1073 472 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1073 472 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2110 2245 2361 2394 2400 2544
Size: 552 939 106 135 116 33 6 144
Cuts at: 2544 2586 2785 2844 3098 3465 513
Size: 42 199 59 254 367 3235
Fragments arranged by size:
3235 939 552 367 254 199 144 135
116 106 59 42 33 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 4497 4517 3703
Size: 794 20 5373
Fragments arranged by size:
5373 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DraIII CAC_nnn'GTG
Cuts at: 2044 2044
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2358 2364
Size: 54 1425 50 275 18 315 213 6
Cuts at: 2364 2391 2397 2403 2419 2448 2487 2679 2880
Size: 27 6 6 16 29 39 192 201
Cuts at: 2880 2955 3118 3317 3468 3634 4523 6152 8
Size: 75 163 199 151 166 889 1629 43
Fragments arranged by size:
1629 1425 889 315 275 213 201 199
192 166 163 151 75 54 50 43
39 29 27 18 16 6 6 6
EagI C'GGCC_G
Cuts at: 1537 1812 2358 2391 2397 2448 2880 2955 3118
Size: 275 546 33 6 51 432 75 163
Cuts at: 3118 3317 3634 1537
Size: 199 317 4090
Fragments arranged by size:
4090 546 432 317 275 199 163 75
51 33 6
EarI CTCTTCn'nnn_
Cuts at: 1353 4117 1353
Size: 2764 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2838 3014 4774 5148 5294 387
Size: 1064 138 1249 176 1760 374 146 1280
Fragments arranged by size:
1760 1280 1249 1064 374 176 146 138
EcoICRI GAG'CTC
Cuts at: 3367 3367
Size: 6187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 3026 4250 4847 5634 5902 1239
Size: 393 407 987 1224 597 787 268 1524
Fragments arranged by size:
1524 1224 987 787 597 407 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3133 3133
Size: 6187
EcoO109I rG'GnC_Cy
Cuts at: 2032 3451 3452 2032
Size: 1419 1 4767
Fragments arranged by size:
4767 1419 1
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FspI TGC'GCA
Cuts at: 3086 4693 3086
Size: 1607 4580
HaeII r_GCGC'y
Cuts at: 1678 2126 2134 2199 2300 2537 2643 2844 2861
Size: 448 8 65 101 237 106 201 17
Cuts at: 2861 3013 3185 3259 3406 3465 5334 1678
Size: 152 172 74 147 59 1869 2531
Fragments arranged by size:
2531 1869 448 237 201 172 152 147
106 101 74 65 59 17 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3037 3069 4828 4860 4902
Size: 32 135 32 1465 32 1759 32 42
Cuts at: 4902 4934 1373
Size: 32 2626
Fragments arranged by size:
2626 1759 1465 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KasI G'GCGC_C
Cuts at: 2122 2130 2195 2296 2639 2857 3402 3461 2122
Size: 8 65 101 343 218 545 59 4848
Fragments arranged by size:
4848 545 343 218 101 65 59 8
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3099 5301 5485 1305
Size: 1794 2202 184 2007
Fragments arranged by size:
2202 2007 1794 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4365
Fragments arranged by size:
4365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 4145 4504 4663 518
Size: 648 1347 1632 359 159 2042
Fragments arranged by size:
2042 1632 1347 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2247 2266 2396 2599 2608 2677 2787
Size: 506 135 19 130 203 9 69 110
Cuts at: 2787 2820 2835 2846 2863 2954 3043 3408 3420
Size: 33 15 11 17 91 89 365 12
Cuts at: 3420 3603 4280 5428 5673 1606
Size: 183 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 506 365 245 203 183
135 130 110 91 89 69 33 19
17 15 12 11 9
NaeI GCC'GGC
Cuts at: 2149 3460 2149
Size: 1311 4876
NarI GG'CG_CC
Cuts at: 2123 2131 2196 2297 2640 2858 3403 3462 2123
Size: 8 65 101 343 218 545 59 4848
Fragments arranged by size:
4848 545 343 218 101 65 59 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 2586 513
Size: 552 939 582 4114
Fragments arranged by size:
4114 939 582 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NgoMIV G'CCGG_C
Cuts at: 2147 3458 2147
Size: 1311 4876
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 2391 2448 3634 2391
Size: 57 1186 4944
Fragments arranged by size:
4944 1186 57
NspI r_CATG'y
Cuts at: 1132 1229 3178 3545 5090 1132
Size: 97 1949 367 1545 2229
Fragments arranged by size:
2229 1949 1545 367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3099 1108
Size: 1991 4196
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 4256 4288 5769 5801 3149
Size: 32 1075 32 1481 32 3535
Fragments arranged by size:
3535 1481 1075 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PspOMI G'GGCC_C
Cuts at: 2260 3451 2260
Size: 1191 4996
PstI C_TGCA'G
Cuts at: 830 2340 3156 3243 3279 3539 830
Size: 1510 816 87 36 260 3478
Fragments arranged by size:
3478 1510 816 260 87 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
SacI G_AGCT'C
Cuts at: 3369 3369
Size: 6187
SacII CC_GC'GG
Cuts at: 2113 2248 2397 2788 2847 2113
Size: 135 149 391 59 5453
Fragments arranged by size:
5453 391 149 135 59
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2336 3013 3152 3239 3275 3535 4670
Size: 219 1291 677 139 87 36 260 1135
Cuts at: 4670 5351 5542 826
Size: 681 191 1471
Fragments arranged by size:
1471 1291 1135 681 677 260 219 191
139 87 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 2697 2697
Size: 6187
SfoI GGC'GCC
Cuts at: 2124 2132 2197 2298 2641 2859 3404 3463 2124
Size: 8 65 101 343 218 545 59 4848
Fragments arranged by size:
4848 545 343 218 101 65 59 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 3458 1700
Size: 1758 4429
SmaI CCC'GGG
Cuts at: 1872 2079 2128 2136 3448 3514 1872
Size: 207 49 8 1312 66 4545
Fragments arranged by size:
4545 1312 207 66 49 8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4291 5192 5454 820
Size: 197 926 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SrfI GCCC'GGGC
Cuts at: 2079 2128 2136 2079
Size: 49 8 6130
Fragments arranged by size:
6130 49 8
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2004 2586 2973 3324 3480 3626 513
Size: 552 939 582 387 351 156 146 3074
Fragments arranged by size:
3074 939 582 552 387 351 156 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4214 4231 4384 4569 5014 325
Size: 1255 2634 17 153 185 445 1498
Fragments arranged by size:
2634 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4433 6145 96
Size: 274 80 33 51 1443 2456 1712 138
Fragments arranged by size:
2456 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 2077 2126 2134 3446 3512 1870
Size: 207 49 8 1312 66 4545
Fragments arranged by size:
4545 1312 207 66 49 8
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I
Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI
SapI SexAI SwaI XcmI