Restriction map for
pFN21AE1247
(Circular) (Six-base) MAPSORT of: pFN21AE1247.seq Check: 2645 from: 1 to: 6151
pFN21AE1247 6151 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3407 3407
Size: 6151
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2133 3363 4214 5598 5866 1632
Size: 501 1230 851 1384 268 1917
Fragments arranged by size:
1917 1384 1230 851 501 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 2087
Size: 32 6119
AlwNI CAG_nnn'CTG
Cuts at: 3019 3046 3894 5466 3019
Size: 27 848 1572 3704
Fragments arranged by size:
3704 1572 848 27
ApaI G_GGCC'C
Cuts at: 2376 2376
Size: 6151
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 3460 3686 3838 5947 1493
Size: 1967 226 152 2109 1697
Fragments arranged by size:
2109 1967 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 2102 3481 3848 2102
Size: 1379 367 4405
Fragments arranged by size:
4405 1379 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 3472 4927 618
Size: 325 217 627 252 1433 1455 1842
Fragments arranged by size:
1842 1455 1433 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2022 2376 2486 3351 3470 1391
Size: 631 354 110 865 119 4072
Fragments arranged by size:
4072 865 631 354 119 110
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 6151
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 928
Size: 971 279 4901
Fragments arranged by size:
4901 971 279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2349 4043 5253 2349
Size: 1694 1210 3247
Fragments arranged by size:
3247 1694 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 4762 136
Size: 107 122 71 631 605 376 2714 1525
Fragments arranged by size:
2714 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 2376 2952 4212 5368 1790
Size: 586 576 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 586 576
BmgBI CAC'GTC
Cuts at: 1318 2803 1318
Size: 1485 4666
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2977 3882 4840
Size: 416 53 83 399 24 1560 905 958
Cuts at: 4840 442
Size: 1753
Fragments arranged by size:
1753 1560 958 905 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2714 2804 2942 3041 3200 3266 3278
Size: 968 507 90 138 99 159 66 12
Cuts at: 3278 4811 1239
Size: 1533 2579
Fragments arranged by size:
2579 1533 968 507 159 138 99 90
66 12
Bpu10I CC'TnA_GC
Cuts at: 2504 3326 2504
Size: 822 5329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3423 4276 5141 5439 3423
Size: 853 865 298 4135
Fragments arranged by size:
4135 865 853 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3124 4814 882
Size: 617 1625 1690 2219
Fragments arranged by size:
2219 1690 1625 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 2886 6145 2886
Size: 3259 2892
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3958 4340 4975
Size: 53 83 186 936 507 1918 382 635
Cuts at: 4975 275
Size: 1451
Fragments arranged by size:
1918 1451 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2309 2339 2415
Size: 30 165 30 834 30 42 30 76
Cuts at: 2415 2445 3014 3044 3419 3449 1178
Size: 30 569 30 375 30 3880
Fragments arranged by size:
3880 834 569 375 165 76 42 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2073 2258 2276 2312 2330 2474 2510 2591
Size: 887 185 18 36 18 144 36 81
Cuts at: 2591 2729 3038 3041 1186
Size: 138 309 3 4296
Fragments arranged by size:
4296 887 309 185 144 138 81 36
36 18 18 3
BseYI C'CCAG_C
Cuts at: 2486 2565 2850 2871 3071 5354 5850 2486
Size: 79 285 21 200 2283 496 2787
Fragments arranged by size:
2787 2283 496 285 200 79 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3245 1163
Size: 573 1509 4069
Fragments arranged by size:
4069 1509 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 3351 4212 4297 5368 2409
Size: 942 861 85 1071 3192
Fragments arranged by size:
3192 1071 942 861 85
BsmI GAATG_Cn'
Cuts at: 3662 3755 3662
Size: 93 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2376 2409 2486 2952 3351 3470
Size: 399 232 354 33 77 466 399 119
Cuts at: 3470 4212 4297 5368 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 399 399 354 232
119 85 77 33
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3407 3506 844
Size: 2563 99 3489
Fragments arranged by size:
3489 2563 99
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4646 4820 65
Size: 1183 3398 174 1396
Fragments arranged by size:
3398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2967 4795 4982 1700
Size: 110 1157 1828 187 2869
Fragments arranged by size:
2869 1828 1157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 2198 1184
Size: 1014 5137
BstXI CCAn_nnnn'nTGG
Cuts at: 2945 3385 2945
Size: 440 5711
BstYI r'GATC_y
Cuts at: 1972 2102 2718 3105 3481 3848 4233 4250 5702
Size: 130 616 387 376 367 385 17 1452
Cuts at: 5702 5788 5800 6146 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 616 387 385 376 367 346
130 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2457 2708 2457
Size: 251 5900
BtgI C'CryG_G
Cuts at: 513 1065 2004 2340 2376 513
Size: 552 939 336 36 4288
Fragments arranged by size:
4288 939 552 336 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3667 4461 4481 2552
Size: 1115 794 20 4222
Fragments arranged by size:
4222 1115 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3598 4487
Size: 54 1425 50 275 18 177 1591 889
Cuts at: 4487 6116 8
Size: 1629 43
Fragments arranged by size:
1629 1591 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2568 3213 3339 4081 1353
Size: 738 477 645 126 742 3423
Fragments arranged by size:
3423 742 738 645 477 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 3149 4738 5112 5258 387
Size: 1064 138 867 693 1589 374 146 1280
Fragments arranged by size:
1589 1280 1064 867 693 374 146 138
EcoICRI GAG'CTC
Cuts at: 3349 3349
Size: 6151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2207 2714 2804 2942 3041 3200
Size: 393 501 74 507 90 138 99 159
Cuts at: 3200 3266 3278 3363 4214 4811 5598 5866 1239
Size: 66 12 85 851 597 787 268 1524
Fragments arranged by size:
1524 851 787 597 507 501 393 268
159 138 99 90 85 74 66 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2111 2630 2711 3023 3389 2111
Size: 519 81 312 366 4873
Fragments arranged by size:
4873 519 366 312 81
EcoO109I rG'GnC_Cy
Cuts at: 2157 2248 2372 2515 3016 2157
Size: 91 124 143 501 5292
Fragments arranged by size:
5292 501 143 124 91
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 2043 5298 1678
Size: 365 3255 2531
Fragments arranged by size:
3255 2531 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2415 2447 3067 3099 3419
Size: 32 135 32 843 32 620 32 320
Cuts at: 3419 3421 3451 3453 4792 4824 4866 4898 1373
Size: 2 30 2 1339 32 42 32 2626
Fragments arranged by size:
2626 1339 843 620 320 135 42 32
32 32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2738 748
Size: 1990 4161
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 6151
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5265 5449 1305
Size: 3960 184 2007
Fragments arranged by size:
3960 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4152
Fragments arranged by size:
4152 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4109 4468 4627 518
Size: 648 2943 359 159 2042
Fragments arranged by size:
2943 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2782 2908 3244 3567 4244 5392 5637
Size: 963 213 126 336 323 677 1148 245
Cuts at: 5637 1606
Size: 2120
Fragments arranged by size:
2120 1148 963 677 336 323 245 213
126
NaeI GCC'GGC
Cuts at: 2969 2969
Size: 6151
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 6151
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NgoMIV G'CCGG_C
Cuts at: 2967 2967
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2294 3509 5054 1132
Size: 97 1065 1215 1545 2229
Fragments arranged by size:
2229 1545 1215 1065 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 3019 1108
Size: 1452 459 4240
Fragments arranged by size:
4240 1452 459
PfoI T'CCnGG_A
Cuts at: 2613 2823 2877 2958 3108 2613
Size: 210 54 81 150 5656
Fragments arranged by size:
5656 210 150 81 54
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 4220 4252 5733 5765 3026
Size: 32 1162 32 1481 32 3412
Fragments arranged by size:
3412 1481 1162 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2157 2515 3016 2157
Size: 358 501 5292
Fragments arranged by size:
5292 501 358
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3360 3721 3360
Size: 361 5790
PspOMI G'GGCC_C
Cuts at: 2372 2372
Size: 6151
PstI C_TGCA'G
Cuts at: 830 2631 3090 3503 830
Size: 1801 459 413 3478
Fragments arranged by size:
3478 1801 459 413
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2569 2782 2908 3244 2569
Size: 213 126 336 5476
Fragments arranged by size:
5476 336 213 126
SacI G_AGCT'C
Cuts at: 3351 3351
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SapI GCTCTTCn'nnn_
Cuts at: 3339 3339
Size: 6151
SbfI CC_TGCA'GG
Cuts at: 2631 3503 2631
Size: 872 5279
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 2627 3086 3499 4634 5315 5506 826
Size: 219 1582 459 413 1135 681 191 1471
Fragments arranged by size:
1582 1471 1135 681 459 413 219 191
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 6151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3438 4255 5156 5418 820
Size: 197 926 1495 817 901 262 1553
Fragments arranged by size:
1553 1495 926 901 817 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 3590 513
Size: 552 939 1586 3074
Fragments arranged by size:
3074 1586 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4178 4195 4348 4533 4978 325
Size: 1255 2598 17 153 185 445 1498
Fragments arranged by size:
2598 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3231 4397 6109
Size: 274 80 33 51 1443 1254 1166 1712
Cuts at: 6109 96
Size: 138
Fragments arranged by size:
1712 1443 1254 1166 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 6111 6143 2309
Size: 32 3770 32 2317
Fragments arranged by size:
3770 2317 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3433 1316
Size: 2117 4034
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 2175 2741 4280 2175
Size: 566 1539 4046
Fragments arranged by size:
4046 1539 566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI XcmI