Restriction map for
pFN21AE1245
(Circular) (Six-base) MAPSORT of: pFN21AE1245.seq Check: 1697 from: 1 to: 6136
pFN21AE1245 6136 bp
With 182 enzymes: SgfI *
March 28, 2012 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2755 2755
Size: 6136
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4199 5583 5851 1632
Size: 2567 1384 268 1917
Fragments arranged by size:
2567 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2207 1184
Size: 1023 5113
AlwNI CAG_nnn'CTG
Cuts at: 2509 3172 3879 5451 2509
Size: 663 707 1572 3194
Fragments arranged by size:
3194 1572 707 663
ApaLI G'TGCA_C
Cuts at: 2542 4193 5349 2542
Size: 1651 1156 3329
Fragments arranged by size:
3329 1651 1156
ApoI r'AATT_y
Cuts at: 1493 3445 3671 3823 5932 1493
Size: 1952 226 152 2109 1697
Fragments arranged by size:
2109 1952 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2621 2621
Size: 6136
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 3238 3275 3385 3461 1870
Size: 73 210 1085 37 110 76 4545
Fragments arranged by size:
4545 1085 210 110 76 73 37
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2104 2338 3064 3204
Size: 325 217 627 243 74 234 726 140
Cuts at: 3204 3457 4912 618
Size: 253 1455 1842
Fragments arranged by size:
1842 1455 726 627 325 253 243 234
217 140 74
BanII G_rGCy'C
Cuts at: 1391 2043 3345 3455 1391
Size: 652 1302 110 4072
Fragments arranged by size:
4072 1302 652 110
BbeI G_GCGC'C
Cuts at: 2034 3208 2034
Size: 1174 4962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2992 3076 928
Size: 971 1093 84 3988
Fragments arranged by size:
3988 1093 971 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3159 3193 4327 4361 1903
Size: 34 1222 34 1134 34 3678
Fragments arranged by size:
3678 1222 1134 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 2561 2906
Size: 107 122 71 631 605 418 471 345
Cuts at: 2906 3113 3411 4747 136
Size: 207 298 1336 1525
Fragments arranged by size:
1525 1336 631 605 471 418 345 298
207 122 107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2546 2712 3069 4197 5353 1790
Size: 319 437 166 357 1128 1156 2573
Fragments arranged by size:
2573 1156 1128 437 357 319 166
BmgBI CAC'GTC
Cuts at: 1318 2419 1318
Size: 1101 5035
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4825 442
Size: 416 53 83 399 24 2450 958 1753
Fragments arranged by size:
2450 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3359 4796 1239
Size: 2120 1437 2579
Fragments arranged by size:
2579 2120 1437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2957 4261 5126 5424 2957
Size: 1304 865 298 3669
Fragments arranged by size:
3669 1304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 3216 4799 882
Size: 617 939 778 1583 2219
Fragments arranged by size:
2219 1583 939 778 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 3927 493
Size: 711 1503 1220 2702
Fragments arranged by size:
2702 1503 1220 711
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2261 2997 3205
Size: 53 83 186 936 498 230 736 208
Cuts at: 3205 3943 4325 4960 275
Size: 738 382 635 1451
Fragments arranged by size:
1451 936 738 736 635 498 382 230
208 186 83 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5911
Fragments arranged by size:
5911 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6136
BseYI C'CCAG_C
Cuts at: 2373 2809 2980 3097 5339 5835 2373
Size: 436 171 117 2242 496 2674
Fragments arranged by size:
2674 2242 496 436 171 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5563
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2819 3073 3230 3586 4347
Size: 275 186 60 758 254 157 356 761
Cuts at: 4347 5375 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 758 356 275 254 186
157 60
BsiHKAI G_wGCw'C
Cuts at: 2022 2546 4197 4282 5353 2022
Size: 524 1651 85 1071 2805
Fragments arranged by size:
2805 1651 1071 524 85
BsmI GAATG_Cn'
Cuts at: 3647 3740 3647
Size: 93 6043
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2043 2109 2546 2712 3069 3345
Size: 399 232 21 66 437 166 357 276
Cuts at: 3345 3455 4197 4282 5353 1391
Size: 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 437 399 357 276 232
166 110 85 66 21
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2745 2755 3491 844
Size: 1901 10 736 3489
Fragments arranged by size:
3489 1901 736 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 4023 1394
Size: 429 245 1955 3507
Fragments arranged by size:
3507 1955 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 2236 2457 4631 4805 65
Size: 1183 988 221 2174 174 1396
Fragments arranged by size:
2174 1396 1183 988 221 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3201 3225 3350 4780 4967 1700
Size: 110 1391 24 125 1430 187 2869
Fragments arranged by size:
2869 1430 1391 187 125 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 2518 2520 2621 3183 3185 3232 3329 1925
Size: 593 2 101 562 2 47 97 4732
Fragments arranged by size:
4732 593 562 101 97 47 2 2
BssSI C'ACGA_G
Cuts at: 2537 2945 4196 5208 2537
Size: 408 1251 1012 3465
Fragments arranged by size:
3465 1251 1012 408
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6136
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 2045 1184
Size: 861 5275
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2359
Size: 6136
BstYI r'GATC_y
Cuts at: 1972 3466 3833 4218 4235 5687 5773 5785 6131
Size: 1494 367 385 17 1452 86 12 346
Cuts at: 6131 1972
Size: 1977
Fragments arranged by size:
1977 1494 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2264 3217 513
Size: 552 939 84 176 953 3432
Fragments arranged by size:
3432 953 939 552 176 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2236 2665 3255 487
Size: 683 56 1010 429 590 3368
Fragments arranged by size:
3368 1010 683 590 429 56
BtsI GCAGTG_nn'
Cuts at: 2177 3652 4446 4466 2177
Size: 1475 794 20 3847
Fragments arranged by size:
3847 1475 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DraIII CAC_nnn'GTG
Cuts at: 2550 2550
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3305 3308 5143 809
Size: 507 1989 3 1835 1802
Fragments arranged by size:
1989 1835 1802 507 3
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2082 2247
Size: 54 1425 50 275 18 228 24 165
Cuts at: 2247 2304 2433 2816 2907 3030 3227 3583 4472
Size: 57 129 383 91 123 197 356 889
Cuts at: 4472 6101 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 383 356 275 228 197
165 129 123 91 57 54 50 43
24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2816 3227 3583 1537
Size: 275 246 758 411 356 4090
Fragments arranged by size:
4090 758 411 356 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 3083 4066 1353
Size: 941 789 983 3423
Fragments arranged by size:
3423 983 941 789
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2621 3389 4723 5097 5243 387
Size: 1064 138 1032 768 1334 374 146 1280
Fragments arranged by size:
1334 1280 1064 1032 768 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3359 4199 4796 5583 5851 1239
Size: 393 1727 840 597 787 268 1524
Fragments arranged by size:
1727 1524 840 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2533 2533
Size: 6136
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 2034 2204 2313 2794 2972 3208 5283 1678
Size: 356 170 109 481 178 236 2075 2531
Fragments arranged by size:
2531 2075 481 356 236 178 170 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 3368 3400 4777
Size: 32 135 32 575 32 1189 32 1377
Cuts at: 4777 4809 4851 4883 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1377 1189 575 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KasI G'GCGC_C
Cuts at: 2030 3204 2030
Size: 1174 4962
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5250 5434 1305
Size: 3945 184 2007
Fragments arranged by size:
3945 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 2306 2909 3032 10
Size: 54 1425 343 417 57 603 123 3114
Fragments arranged by size:
3114 1425 603 417 343 123 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2360 2588 2990 4094 4453 4612
Size: 648 1191 3 228 402 1104 359 159
Cuts at: 4612 518
Size: 2042
Fragments arranged by size:
2042 1191 1104 648 402 359 228 159
3
MspA1I CmG'CkG
Cuts at: 1606 2090 2161 2180 2266 2373 2628 2784 2905
Size: 484 71 19 86 107 255 156 121
Cuts at: 2905 3552 4229 5377 5622 1606
Size: 647 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 647 484 255 245 156
121 107 86 71 19
NaeI GCC'GGC
Cuts at: 3203 3352 3203
Size: 149 5987
NarI GG'CG_CC
Cuts at: 2031 3205 2031
Size: 1174 4962
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4645
Fragments arranged by size:
4645 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NgoMIV G'CCGG_C
Cuts at: 3201 3350 3201
Size: 149 5987
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 2816 3583 2816
Size: 767 5369
NspI r_CATG'y
Cuts at: 1132 1229 3494 5039 1132
Size: 97 2265 1545 2229
Fragments arranged by size:
2265 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3345 1348
Size: 1997 4139
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspXI vC'TCGA_Gb
Cuts at: 3385 3385
Size: 6136
PstI C_TGCA'G
Cuts at: 830 2274 3488 830
Size: 1444 1214 3478
Fragments arranged by size:
3478 1444 1214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2784 2784
Size: 6136
SacII CC_GC'GG
Cuts at: 2091 2267 2091
Size: 176 5960
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SexAI A'CCwGG_T
Cuts at: 2316 2316
Size: 6136
SfcI C'TryA_G
Cuts at: 826 1045 2270 3484 4619 5300 5491 826
Size: 219 1225 1214 1135 681 191 1471
Fragments arranged by size:
1471 1225 1214 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2561 2561
Size: 6136
SfoI GGC'GCC
Cuts at: 2032 3206 2032
Size: 1174 4962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 3201 1700
Size: 1501 4635
SmaI CCC'GGG
Cuts at: 1872 3277 3463 1872
Size: 1405 186 4545
Fragments arranged by size:
4545 1405 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2972 3385 4240 5141 5403 820
Size: 197 926 1029 413 855 901 262 1553
Fragments arranged by size:
1553 1029 926 901 855 413 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 3047 3161 1517
Size: 1530 114 4492
Fragments arranged by size:
4492 1530 114
StyI C'CwwG_G
Cuts at: 513 1065 2004 3429 3575 513
Size: 552 939 1425 146 3074
Fragments arranged by size:
3074 1425 939 552 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4163 4180 4333 4518 4963 325
Size: 1255 2583 17 153 185 445 1498
Fragments arranged by size:
2583 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2408 2440 3386 3418 6096 6128 2408
Size: 32 946 32 2678 32 2416
Fragments arranged by size:
2678 2416 946 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2318 2657 3305 1316
Size: 1002 339 648 4147
Fragments arranged by size:
4147 1002 648 339
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2314 2563 2902 2314
Size: 249 339 5548
Fragments arranged by size:
5548 339 249
XhoI C'TCGA_G
Cuts at: 1943 3385 1943
Size: 1442 4694
XmaI C'CCGG_G
Cuts at: 1870 3275 3461 1870
Size: 1405 186 4545
Fragments arranged by size:
4545 1405 186
XmnI GAAnn'nnTTC
Cuts at: 3047 4265 3047
Size: 1218 4918
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PsrI RsrII SacI SanDI SapI SrfI SwaI