Restriction map for
pFN21AE1224
(Circular) (Six-base) MAPSORT of: pFN21AE1224.seq Check: 3500 from: 1 to: 5518
pFN21AE1224 5518 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 2685 3581 4965 5233 1632
Size: 839 214 896 1384 268 1917
Fragments arranged by size:
1917 1384 896 839 268 214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2417 2449 2417
Size: 32 5486
AlwNI CAG_nnn'CTG
Cuts at: 2543 2736 3261 4833 2543
Size: 193 525 1572 3228
Fragments arranged by size:
3228 1572 525 193
ApaI G_GGCC'C
Cuts at: 2555 2555
Size: 5518
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2350 2843 1870
Size: 73 407 493 4545
Fragments arranged by size:
4545 493 407 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2411 2573 2743 2839 4294
Size: 325 217 627 624 162 170 96 1455
Cuts at: 4294 618
Size: 1842
Fragments arranged by size:
1842 1455 627 624 325 217 170 162
96
BanII G_rGCy'C
Cuts at: 1391 2555 2613 2837 1391
Size: 1164 58 224 4072
Fragments arranged by size:
4072 1164 224 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2331 2770 4129
Size: 107 122 71 631 605 659 439 1359
Cuts at: 4129 136
Size: 1525
Fragments arranged by size:
1525 1359 659 631 605 439 122 107
71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2414 2555 2748 3579 4735 1790
Size: 624 141 193 831 1156 2573
Fragments arranged by size:
2573 1156 831 624 193 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2225 3249 4207
Size: 416 53 83 399 24 808 1024 958
Cuts at: 4207 442
Size: 1753
Fragments arranged by size:
1753 1024 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2458 2627 2674 2713 4178 1239
Size: 1219 169 47 39 1465 2579
Fragments arranged by size:
2579 1465 1219 169 47 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2496 3309 493
Size: 711 1292 813 2702
Fragments arranged by size:
2702 1292 813 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3325 3707 4342 275
Size: 53 83 186 936 1792 382 635 1451
Fragments arranged by size:
1792 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3951 4349 4623 4770 5181 3951
Size: 398 274 147 411 4288
Fragments arranged by size:
4288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5518
BseYI C'CCAG_C
Cuts at: 2203 2247 4721 5217 2203
Size: 44 2474 496 2504
Fragments arranged by size:
2504 2474 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4945
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3579 3664 4735 3579
Size: 85 1071 4362
Fragments arranged by size:
4362 1071 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
BsmBI CGTCTCn'nnnn_
Cuts at: 2652 2652
Size: 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2414 2555 2613 2748 2837 3579 3664
Size: 399 624 141 58 135 89 742 85
Cuts at: 3664 4735 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 624 399 141 135 89
85 58
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 844
Size: 2029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2650 3405 1394
Size: 429 827 755 3507
Fragments arranged by size:
3507 827 755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2080 4162 4349 1700
Size: 110 246 24 2082 187 2869
Fragments arranged by size:
2869 2082 246 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2724 794
Size: 1930 3588
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 2164 1184
Size: 980 4538
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2016 2016
Size: 5518
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4966
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 3034 3828 3848 2118
Size: 916 794 20 3788
Fragments arranged by size:
3788 916 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2674 2709 529
Size: 35 2110 35 3338
Fragments arranged by size:
3338 2110 35 35
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2231 4525 809
Size: 507 915 2294 1802
Fragments arranged by size:
2294 1802 915 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2965 3854
Size: 54 1425 50 275 18 248 887 889
Cuts at: 3854 5483 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 887 275 248 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3448 1353
Size: 2095 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2458 2471 2627 2674 2685 2713 3581
Size: 393 826 13 156 47 11 28 868
Cuts at: 3581 4178 4965 5233 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 868 826 787 597 393 268 156
47 28 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2038 2038
Size: 5518
EcoO109I rG'GnC_Cy
Cuts at: 2136 2240 2251 2382 2551 2552 2602 2643 2673
Size: 104 11 131 169 1 50 41 30
Cuts at: 2673 2136
Size: 4981
Fragments arranged by size:
4981 169 131 104 50 41 30 11
1
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 2620 4665 1678
Size: 942 2045 2531
Fragments arranged by size:
2531 2045 942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2191 2223 4159
Size: 32 135 32 427 32 160 32 1936
Cuts at: 4159 4191 4233 4265 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1936 427 160 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2047 2612 4632 4816 1305
Size: 742 565 2020 184 2007
Fragments arranged by size:
2020 2007 742 565 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2395 2733 2934 3611 4759 5004 1606
Size: 789 338 201 677 1148 245 2120
Fragments arranged by size:
2120 1148 789 677 338 245 201
NaeI GCC'GGC
Cuts at: 2058 2082 2058
Size: 24 5494
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4966
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2056 2080 2056
Size: 24 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2154 2876 4421 1132
Size: 97 925 722 1545 2229
Fragments arranged by size:
2229 1545 925 722 97
PasI CC'CwG_GG
Cuts at: 1348 2429 1348
Size: 1081 4437
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2736 1108
Size: 1628 3890
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2136 2251 2643 2673 2136
Size: 115 392 30 4981
Fragments arranged by size:
4981 392 115 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2551 2551
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2346 2484 2513 2539 2560 2870 830
Size: 1516 138 29 26 21 310 3478
Fragments arranged by size:
3478 1516 310 138 29 26 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2395 2733 2395
Size: 338 5180
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SanDI GG'GwC_CC
Cuts at: 2251 2673 2251
Size: 422 5096
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SfcI C'TryA_G
Cuts at: 826 1045 2342 2480 2509 2535 2556 2866 4001
Size: 219 1297 138 29 26 21 310 1135
Cuts at: 4001 4682 4873 826
Size: 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 310 219 191 138
29 26 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2352 2845 1872
Size: 480 493 4545
Fragments arranged by size:
4545 493 480
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2154 2876 1132
Size: 1022 722 3774
Fragments arranged by size:
3774 1022 722
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2039 2301 2957 513
Size: 552 974 262 656 3074
Fragments arranged by size:
3074 974 656 552 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 2152 2580 3545 3562 3715 3900
Size: 1255 535 37 428 965 17 153 185
Cuts at: 3900 4345 325
Size: 445 1498
Fragments arranged by size:
1498 1255 965 535 445 428 185 153
37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 2737
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2350 2843 1870
Size: 480 493 4545
Fragments arranged by size:
4545 493 480
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI