Restriction map for
pFN21AE1219
(Circular) (Six-base) MAPSORT of: pFN21AE1219.seq Check: 8646 from: 1 to: 5374
pFN21AE1219 5374 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2472 2717 1937
Size: 535 245 4594
Fragments arranged by size:
4594 535 245
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 3437 4821 5089 1632
Size: 610 1195 1384 268 1917
Fragments arranged by size:
1917 1384 1195 610 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 2152 3117 4689 2152
Size: 965 1572 2837
Fragments arranged by size:
2837 1572 965
ApaLI G'TGCA_C
Cuts at: 2012 2236 3431 4587 2012
Size: 224 1195 1156 2799
Fragments arranged by size:
2799 1195 1156 224
ApoI r'AATT_y
Cuts at: 1493 2424 2683 2909 3061 5170 1493
Size: 931 259 226 152 2109 1697
Fragments arranged by size:
2109 1697 931 259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2180 2699 1870
Size: 73 194 43 519 4545
Fragments arranged by size:
4545 519 194 73 43
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2695 4150 618
Size: 325 217 627 908 1455 1842
Fragments arranged by size:
1842 1455 908 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2195 2234 2643 2693 1391
Size: 804 39 409 50 4072
Fragments arranged by size:
4072 804 409 50 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3565 3599 1903
Size: 34 50 34 1544 34 3678
Fragments arranged by size:
3678 1544 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BfrBI ATG'CAT
Cuts at: 2311 2311
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 2624 3985
Size: 107 122 71 631 605 364 588 1361
Cuts at: 3985 136
Size: 1525
Fragments arranged by size:
1525 1361 631 605 588 364 122 107
71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2057 2339 2642 2791 2057
Size: 282 303 149 4640
Fragments arranged by size:
4640 303 282 149
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2240 3435 4591 1790
Size: 226 224 1195 1156 2573
Fragments arranged by size:
2573 1195 1156 226 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 4063 442
Size: 416 53 83 399 24 1688 958 1753
Fragments arranged by size:
1753 1688 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2285 4034 1239
Size: 905 141 1749 2579
Fragments arranged by size:
2579 1749 905 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3181 3563 4198 275
Size: 53 83 186 936 1648 382 635 1451
Fragments arranged by size:
1648 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2433 2463 2495 2525 1178
Size: 30 165 30 1030 30 32 30 4027
Fragments arranged by size:
4027 1030 165 32 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 2396 2399 2537 1186
Size: 1195 15 3 138 4023
Fragments arranged by size:
4023 1195 138 15 3
BseYI C'CCAG_C
Cuts at: 2155 2459 4577 5073 2155
Size: 304 2118 496 2456
Fragments arranged by size:
2456 2118 496 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 1163
Size: 573 510 4291
Fragments arranged by size:
4291 573 510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2824 3585 4613 1540
Size: 275 186 78 745 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2016 2070 2195 2240 2643 3435 3520 4591 2016
Size: 54 125 45 403 792 85 1071 2799
Fragments arranged by size:
2799 1071 792 403 125 85 54 45
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
BsmBI CGTCTCn'nnnn_
Cuts at: 2644 2644
Size: 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2070 2195 2234 2240 2643 2693
Size: 399 226 54 125 39 6 403 50
Cuts at: 2693 3435 3520 4591 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 226 125 85
54 50 39 6
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2225 2729 844
Size: 1316 65 504 3489
Fragments arranged by size:
3489 1316 504 65
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 2338 3261 1394
Size: 429 296 219 923 3507
Fragments arranged by size:
3507 923 429 296 219
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2292 4018 4205 1700
Size: 110 220 262 1726 187 2869
Fragments arranged by size:
2869 1726 262 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2152 794
Size: 1358 4016
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 2449 1184
Size: 1265 4109
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3442
Fragments arranged by size:
3442 1380 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2578 487
Size: 683 56 1352 3283
Fragments arranged by size:
3283 1352 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3684 3704 2890
Size: 794 20 4560
Fragments arranged by size:
4560 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2076 2821
Size: 54 1425 50 275 18 198 48 745
Cuts at: 2821 3710 5339 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 745 275 198 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2076 2821 1537
Size: 275 264 745 4090
Fragments arranged by size:
4090 745 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 3304 1353
Size: 1082 869 3423
Fragments arranged by size:
3423 1082 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2227 3961 4335 4481 387
Size: 1064 138 638 1734 374 146 1280
Fragments arranged by size:
1734 1280 1064 638 374 146 138
EcoICRI GAG'CTC
Cuts at: 2193 2641 2193
Size: 448 4926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2242 2285 3437 4034 4821 5089
Size: 393 512 98 43 1152 597 787 268
Cuts at: 5089 1239
Size: 1524
Fragments arranged by size:
1524 1152 787 597 512 393 268 98
43
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2540
Size: 5374
EcoO109I rG'GnC_Cy
Cuts at: 2149 2530 2149
Size: 381 4993
EcoRI G'AATT_C
Cuts at: 2424 2683 3061 2424
Size: 259 378 4737
Fragments arranged by size:
4737 378 259
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2057 2510 4521 1678
Size: 379 453 2011 2531
Fragments arranged by size:
2531 2011 453 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373
Size: 32 135 32 2443 32 42 32 2626
Fragments arranged by size:
2626 2443 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3552
Fragments arranged by size:
3552 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3332 3691 3850 518
Size: 648 843 1323 359 159 2042
Fragments arranged by size:
2042 1323 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2790 3467 4615 4860 1606
Size: 639 545 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 545 245
NaeI GCC'GGC
Cuts at: 2032 2294 2032
Size: 262 5112
NcoI C'CATG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3442
Fragments arranged by size:
3442 1380 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NgoMIV G'CCGG_C
Cuts at: 2030 2292 2030
Size: 262 5112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2076 2821 2076
Size: 745 4629
NsiI A_TGCA'T
Cuts at: 2313 2313
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 2575 1348
Size: 1227 4147
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5374
PfoI T'CCnGG_A
Cuts at: 2499 2535 2499
Size: 36 5338
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2149
Size: 5374
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2726 830
Size: 1896 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
SacI G_AGCT'C
Cuts at: 2195 2643 2195
Size: 448 4926
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SexAI A'CCwGG_T
Cuts at: 2541 2541
Size: 5374
SfcI C'TryA_G
Cuts at: 826 1045 2722 3857 4538 4729 826
Size: 219 1677 1135 681 191 1471
Fragments arranged by size:
1677 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2139 2701 1872
Size: 267 562 4545
Fragments arranged by size:
4545 562 267
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2445 2813 513
Size: 552 1380 368 3074
Fragments arranged by size:
3074 1380 552 368
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3418 3571 3756 4201 325
Size: 1255 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 5332 96
Size: 274 80 33 51 1443 1643 1712 138
Fragments arranged by size:
1712 1643 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2433 2465 2495 2527 5334 5366 2433
Size: 32 30 32 2807 32 2441
Fragments arranged by size:
2807 2441 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2137 2699 1870
Size: 267 562 4545
Fragments arranged by size:
4545 562 267
XmnI GAAnn'nnTTC
Cuts at: 2255 3503 2255
Size: 1248 4126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI