Restriction map for
pFN21AE1195
(Circular) (Six-base) MAPSORT of: pFN21AE1195.seq Check: 4983 from: 1 to: 7189
pFN21AE1195 7189 bp
With 182 enzymes: SgfI *
March 28, 2012 12:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4999 278
Size: 53 83 186 936 3463 2468
Fragments arranged by size:
3463 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2673 4532 1937
Size: 736 1859 4594
Fragments arranged by size:
4594 1859 736
Acc65I G'GTAC_C
Cuts at: 4510 4510
Size: 7189
AclI AA'CG_TT
Cuts at: 1563 3569 5316 5689 1563
Size: 2006 1747 373 3063
Fragments arranged by size:
3063 2006 1747 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 3335 3729 3934 4407 5252 6636 6904
Size: 402 1301 394 205 473 845 1384 268
Cuts at: 6904 1632
Size: 1917
Fragments arranged by size:
1917 1384 1301 845 473 402 394 268
205
AflII C'TTAA_G
Cuts at: 820 1017 3119 820
Size: 197 2102 4890
Fragments arranged by size:
4890 2102 197
AflIII A'CryG_T
Cuts at: 1203 6088 7065 1203
Size: 4885 977 1327
Fragments arranged by size:
4885 1327 977
AgeI A'CCGG_T
Cuts at: 6020 6020
Size: 7189
AhdI GACnn_n'nnGTC
Cuts at: 2698 3515 5918 2698
Size: 817 2403 3969
Fragments arranged by size:
3969 2403 817
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3600 1184
Size: 2416 4773
AlwNI CAG_nnn'CTG
Cuts at: 2838 3487 4932 6504 2838
Size: 649 1445 1572 3523
Fragments arranged by size:
3523 1572 1445 649
ApaLI G'TGCA_C
Cuts at: 5246 6402 5246
Size: 1156 6033
ApoI r'AATT_y
Cuts at: 1493 2408 2430 2722 2786 2948 3039 3049 4181
Size: 915 22 292 64 162 91 10 1132
Cuts at: 4181 4391 4411 4498 4724 4876 6985 1493
Size: 210 20 87 226 152 2109 1697
Fragments arranged by size:
2109 1697 1132 915 292 226 210 162
152 91 87 64 22 20 10
AseI AT'TA_AT
Cuts at: 160 1032 5743 160
Size: 872 4711 1606
Fragments arranged by size:
4711 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
AvaI C'yCGr_G
Cuts at: 1870 1943 4514 1870
Size: 73 2571 4545
Fragments arranged by size:
4545 2571 73
BamHI G'GATC_C
Cuts at: 3901 4519 4886 3901
Size: 618 367 6204
Fragments arranged by size:
6204 618 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3204 4510 5965 618
Size: 325 217 627 1417 1306 1455 1842
Fragments arranged by size:
1842 1455 1417 1306 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4508 1391
Size: 3117 4072
BbeI G_GCGC'C
Cuts at: 3208 3208
Size: 7189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2546 2694 2820 2834 4358 928
Size: 971 647 148 126 14 1524 3759
Fragments arranged by size:
3759 1524 971 647 148 126 14
BbvCI CC'TCA_GC
Cuts at: 3588 3588
Size: 7189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5380 5414 1903
Size: 34 3443 34 3678
Fragments arranged by size:
3678 3443 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5081 6291 5081
Size: 1210 5979
BclI T'GATC_A
Cuts at: 1548 2266 1548
Size: 718 6471
BfrBI ATG'CAT
Cuts at: 2767 2767
Size: 7189
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4158 5800
Size: 107 122 71 631 605 337 2149 1642
Cuts at: 5800 136
Size: 1525
Fragments arranged by size:
2149 1642 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 7184 7184
Size: 7189
BlpI GC'TnA_GC
Cuts at: 4606 4606
Size: 7189
Bme1580I G_kGCm'C
Cuts at: 1790 2065 5250 6406 1790
Size: 275 3185 1156 2573
Fragments arranged by size:
3185 2573 1156 275
BmgBI CAC'GTC
Cuts at: 1318 2044 2615 1318
Size: 726 571 5892
Fragments arranged by size:
5892 726 571
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4920 5878 442
Size: 416 53 83 399 24 3503 958 1753
Fragments arranged by size:
3503 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3598 4371 3598
Size: 773 6416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3009 4030 5849 1239
Size: 1770 1021 1819 2579
Fragments arranged by size:
2579 1819 1770 1021
Bpu10I CC'TnA_GC
Cuts at: 3588 3588
Size: 7189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5314 6179 6477 5314
Size: 865 298 6026
Fragments arranged by size:
6026 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3516 5852 882
Size: 617 2017 2336 2219
Fragments arranged by size:
2336 2219 2017 617
BsaAI yAC'GTr
Cuts at: 493 1204 4980 493
Size: 711 3776 2702
Fragments arranged by size:
3776 2702 711
BsaBI GATnn'nnATC
Cuts at: 2112 3993 7183 2112
Size: 1881 3190 2118
Fragments arranged by size:
3190 2118 1881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3205 4996 5378 6013
Size: 53 83 186 936 1672 1791 382 635
Cuts at: 6013 275
Size: 1451
Fragments arranged by size:
1791 1672 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2248 5622 6020 6294 6441 6852 2248
Size: 3374 398 274 147 411 2585
Fragments arranged by size:
3374 2585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4250 4280 1178
Size: 30 165 30 2847 30 4087
Fragments arranged by size:
4087 2847 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3605 4065 1186
Size: 2419 460 4310
Fragments arranged by size:
4310 2419 460
BseYI C'CCAG_C
Cuts at: 3480 6392 6888 3480
Size: 2912 496 3781
Fragments arranged by size:
3781 2912 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3873 1163
Size: 573 2137 4479
Fragments arranged by size:
4479 2137 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4639 5400 6428 1540
Size: 275 186 2638 761 1028 2301
Fragments arranged by size:
2638 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5250 5335 6406 5250
Size: 85 1071 6033
Fragments arranged by size:
6033 1071 85
BsmI GAATG_Cn'
Cuts at: 2638 4700 4793 2638
Size: 2062 93 5034
Fragments arranged by size:
5034 2062 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3502 3502
Size: 7189
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 4508 5250 5335 6406 1391
Size: 399 275 2443 742 85 1071 2174
Fragments arranged by size:
2443 2174 1071 742 399 275 85
BspEI T'CCGG_A
Cuts at: 6852 6852
Size: 7189
BspHI T'CATG_A
Cuts at: 5078 6808 5078
Size: 1730 5459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4133 4544 844
Size: 3289 411 3489
Fragments arranged by size:
3489 3289 411
BsrBI CCG'CTC
Cuts at: 1394 1823 5076 1394
Size: 429 3253 3507
Fragments arranged by size:
3507 3253 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2790 2917 5684 5858 65
Size: 1183 1542 127 2767 174 1396
Fragments arranged by size:
2767 1542 1396 1183 174 127
BsrFI r'CCGG_y
Cuts at: 1700 1810 2543 5833 6020 1700
Size: 110 733 3290 187 2869
Fragments arranged by size:
3290 2869 733 187 110
BsrGI T'GTAC_A
Cuts at: 96 4469 96
Size: 4373 2816
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7189
BssSI C'ACGA_G
Cuts at: 5249 6261 5249
Size: 1012 6177
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3496 794
Size: 2702 4487
BstBI TT'CG_AA
Cuts at: 5993 5993
Size: 7189
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7189
BstXI CCAn_nnnn'nTGG
Cuts at: 4448 4448
Size: 7189
BstYI r'GATC_y
Cuts at: 1972 3901 4519 4886 5271 5288 6740 6826 6838
Size: 1929 618 367 385 17 1452 86 12
Cuts at: 6838 7184 1972
Size: 346 1977
Fragments arranged by size:
1977 1929 1452 618 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3527 4284 513
Size: 552 939 1523 757 3418
Fragments arranged by size:
3418 1523 939 757 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6450
Fragments arranged by size:
6450 683 56
BtsI GCAGTG_nn'
Cuts at: 2737 4705 5499 5519 2737
Size: 1968 794 20 4407
Fragments arranged by size:
4407 1968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7154
DraI TTT'AAA
Cuts at: 2494 3642 4493 2494
Size: 1148 851 5190
Fragments arranged by size:
5190 1148 851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6196 809
Size: 507 4880 1802
Fragments arranged by size:
4880 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2413 2545 4636
Size: 54 1425 50 275 18 583 132 2091
Cuts at: 4636 5525 7154 8
Size: 889 1629 43
Fragments arranged by size:
2091 1629 1425 889 583 275 132 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4636 1537
Size: 275 2824 4090
Fragments arranged by size:
4090 2824 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2175 2291 2465 3108 3601 3606 4455
Size: 657 165 116 174 643 493 5 849
Cuts at: 4455 5119 1353
Size: 664 3423
Fragments arranged by size:
3423 849 664 657 643 493 174 165
116 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 5776 6150 6296 387
Size: 1064 138 447 3740 374 146 1280
Fragments arranged by size:
3740 1280 1064 447 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 3009 3335 3729 3934 4030 4407
Size: 393 402 975 326 394 205 96 377
Cuts at: 4407 5252 5849 6636 6904 1239
Size: 845 597 787 268 1524
Fragments arranged by size:
1524 975 845 787 597 402 394 393
377 326 268 205 96
EcoRI G'AATT_C
Cuts at: 2722 2786 4391 4498 4876 2722
Size: 64 1605 107 378 5035
Fragments arranged by size:
5035 1605 378 107 64
EcoRV GAT'ATC
Cuts at: 2032 2032
Size: 7189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2279 3134 2279
Size: 855 6334
FspI TGC'GCA
Cuts at: 5695 5695
Size: 7189
HaeII r_GCGC'y
Cuts at: 1678 3208 6336 1678
Size: 1530 3128 2531
Fragments arranged by size:
3128 2531 1530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 2909 2941 3501
Size: 32 135 32 1112 32 193 32 560
Cuts at: 3501 3533 3893 3925 4066 4098 5830 5862 5904
Size: 32 360 32 141 32 1732 32 42
Cuts at: 5904 5936 1373
Size: 32 2626
Fragments arranged by size:
2626 1732 1112 560 360 193 141 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3427 4533 4779 669
Size: 988 281 1489 1106 246 3079
Fragments arranged by size:
3079 1489 1106 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7189
HpaI GTT'AAC
Cuts at: 4779 4779
Size: 7189
KasI G'GCGC_C
Cuts at: 3204 3204
Size: 7189
KpnI G_GTAC'C
Cuts at: 4514 4514
Size: 7189
MfeI C'AATT_G
Cuts at: 2928 4372 4788 2928
Size: 1444 416 5329
Fragments arranged by size:
5329 1444 416
MluI A'CGCG_T
Cuts at: 7065 7065
Size: 7189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 3706 6303 6487 1305
Size: 888 1513 2597 184 2007
Fragments arranged by size:
2597 2007 1513 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5367
Fragments arranged by size:
5367 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2599 3777 4070 5147 5506 5665 518
Size: 648 1433 1178 293 1077 359 159 2042
Fragments arranged by size:
2042 1433 1178 1077 648 359 293 159
MspA1I CmG'CkG
Cuts at: 1606 4605 5282 6430 6675 1606
Size: 2999 677 1148 245 2120
Fragments arranged by size:
2999 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3205 3205
Size: 7189
NcoI C'CATG_G
Cuts at: 513 1065 2004 3527 4284 513
Size: 552 939 1523 757 3418
Fragments arranged by size:
3418 1523 939 757 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7189
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7189
NotI GC'GGCC_GC
Cuts at: 4636 4636
Size: 7189
NsiI A_TGCA'T
Cuts at: 2769 2769
Size: 7189
NspI r_CATG'y
Cuts at: 1132 1229 3776 4547 6092 1132
Size: 97 2547 771 1545 2229
Fragments arranged by size:
2547 2229 1545 771 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7189
PciI A'CATG_T
Cuts at: 6088 6088
Size: 7189
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2075 1108
Size: 967 6222
PmeI GTTT'AAAC
Cuts at: 4493 4493
Size: 7189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5258 5290 6771 6803 5258
Size: 32 1481 32 5644
Fragments arranged by size:
5644 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7189
PsiI TTA'TAA
Cuts at: 4136 4431 4759 4136
Size: 295 328 6566
Fragments arranged by size:
6566 328 295
PstI C_TGCA'G
Cuts at: 830 2307 3492 4541 830
Size: 1477 1185 1049 3478
Fragments arranged by size:
3478 1477 1185 1049
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7189
SalI G'TCGA_C
Cuts at: 1936 4531 1936
Size: 2595 4594
SapI GCTCTTCn'nnn_
Cuts at: 3108 3108
Size: 7189
SbfI CC_TGCA'GG
Cuts at: 4541 4541
Size: 7189
ScaI AGT'ACT
Cuts at: 5437 7149 5437
Size: 1712 5477
SfcI C'TryA_G
Cuts at: 826 1045 2303 3488 3887 4537 5672 6353 6544
Size: 219 1258 1185 399 650 1135 681 191
Cuts at: 6544 826
Size: 1471
Fragments arranged by size:
1471 1258 1185 1135 681 650 399 219
191
SfoI GGC'GCC
Cuts at: 3206 3206
Size: 7189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7189
SmaI CCC'GGG
Cuts at: 1872 4516 1872
Size: 2644 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3119 5293 6194 6456 820
Size: 197 926 1176 2174 901 262 1553
Fragments arranged by size:
2174 1553 1176 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4980 493
Size: 4487 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7189
SphI G_CATG'C
Cuts at: 1132 3776 4547 1132
Size: 2644 771 3774
Fragments arranged by size:
3774 2644 771
SspI AAT'ATT
Cuts at: 5 52 2225 5113 5
Size: 47 2173 2888 2081
Fragments arranged by size:
2888 2173 2081 47
StuI AGG'CCT
Cuts at: 1517 3923 1517
Size: 2406 4783
StyI C'CwwG_G
Cuts at: 513 1065 2004 2153 2160 3527 3933 4284 4628
Size: 552 939 149 7 1367 406 351 344
Cuts at: 4628 513
Size: 3074
Fragments arranged by size:
3074 1367 939 552 406 351 344 149
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5216 5233 5386 5571 6016 325
Size: 1255 3636 17 153 185 445 1498
Fragments arranged by size:
3636 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 4189 4469
Size: 274 80 33 51 1443 1901 311 280
Cuts at: 4469 5435 7147 96
Size: 966 1712 138
Fragments arranged by size:
1901 1712 1443 966 311 280 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7149 7181 7149
Size: 32 7157
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7189
XbaI T'CTAG_A
Cuts at: 4525 4525
Size: 7189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3534 4378 3534
Size: 844 6345
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7189
XmaI C'CCGG_G
Cuts at: 1870 4514 1870
Size: 2644 4545
XmnI GAAnn'nnTTC
Cuts at: 5318 5318
Size: 7189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4997 276
Size: 53 83 186 936 3463 2468
Fragments arranged by size:
3463 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI