Restriction map for
pFN21AE1177
(Circular) (Six-base) MAPSORT of: pFN21AE1177.seq Check: 2876 from: 1 to: 6757
pFN21AE1177 6757 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4567 278
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2413 4100 1937
Size: 476 1687 4594
Fragments arranged by size:
4594 1687 476
Acc65I G'GTAC_C
Cuts at: 2725 4078 2725
Size: 1353 5404
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2553 3324 4820 6204 6472 1632
Size: 921 771 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 921 771 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6560
AflIII A'CryG_T
Cuts at: 1203 2599 3485 5656 6633 1203
Size: 1396 886 2171 977 1327
Fragments arranged by size:
2171 1396 1327 977 886
AgeI A'CCGG_T
Cuts at: 5588 5588
Size: 6757
AhdI GACnn_n'nnGTC
Cuts at: 5486 5486
Size: 6757
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6757
AlwNI CAG_nnn'CTG
Cuts at: 2345 3143 4500 6072 2345
Size: 798 1357 1572 3030
Fragments arranged by size:
3030 1572 1357 798
ApaI G_GGCC'C
Cuts at: 3752 3752
Size: 6757
ApaLI G'TGCA_C
Cuts at: 4814 5970 4814
Size: 1156 5601
ApoI r'AATT_y
Cuts at: 1493 2474 2547 3054 4066 4292 4444 6553 1493
Size: 981 73 507 1012 226 152 2109 1697
Fragments arranged by size:
2109 1697 1012 981 507 226 152 73
AseI AT'TA_AT
Cuts at: 160 1032 5311 160
Size: 872 4279 1606
Fragments arranged by size:
4279 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 4082 1870
Size: 73 191 1948 4545
Fragments arranged by size:
4545 1948 191 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2717 2750 2717
Size: 33 6724
BamHI G'GATC_C
Cuts at: 4087 4454 4087
Size: 367 6390
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2149 2725 2873 3382 4078
Size: 325 217 627 362 576 148 509 696
Cuts at: 4078 5533 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 696 627 576 509 362 325
217 148
BanII G_rGCy'C
Cuts at: 1391 3752 4076 1391
Size: 2361 324 4072
Fragments arranged by size:
4072 2361 324
BbeI G_GCGC'C
Cuts at: 2153 2153
Size: 6757
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 928
Size: 971 930 4856
Fragments arranged by size:
4856 971 930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4948 4982 1903
Size: 34 3011 34 3678
Fragments arranged by size:
3678 3011 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4649 5859 4649
Size: 1210 5547
BclI T'GATC_A
Cuts at: 1548 2170 1548
Size: 622 6135
BfrBI ATG'CAT
Cuts at: 2229 3128 2229
Size: 899 5858
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 2256 5368
Size: 107 122 71 631 605 475 109 3112
Cuts at: 5368 136
Size: 1525
Fragments arranged by size:
3112 1525 631 605 475 122 109 107
71
BglII A'GATC_T
Cuts at: 6752 6752
Size: 6757
BlpI GC'TnA_GC
Cuts at: 2980 4174 2980
Size: 1194 5563
Bme1580I G_kGCm'C
Cuts at: 1790 3752 4818 5974 1790
Size: 1962 1066 1156 2573
Fragments arranged by size:
2573 1962 1156 1066
BmgBI CAC'GTC
Cuts at: 1318 3486 3880 1318
Size: 2168 394 4195
Fragments arranged by size:
4195 2168 394
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4488 5446 442
Size: 416 53 83 399 24 3071 958 1753
Fragments arranged by size:
3071 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 2346 5417 1239
Size: 969 138 3071 2579
Fragments arranged by size:
3071 2579 969 138
Bpu10I CC'TnA_GC
Cuts at: 3684 3684
Size: 6757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3041 4882 5747 6045 3041
Size: 1841 865 298 3753
Fragments arranged by size:
3753 1841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5420 882
Size: 617 3921 2219
Fragments arranged by size:
3921 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4548 493
Size: 711 3344 2702
Fragments arranged by size:
3344 2702 711
BsaBI GATnn'nnATC
Cuts at: 6751 6751
Size: 6757
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2150 4564 4946 5581
Size: 53 83 186 936 617 2414 382 635
Cuts at: 5581 275
Size: 1451
Fragments arranged by size:
2414 1451 936 635 617 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5190 5588 5862 6009 6420 5190
Size: 398 274 147 411 5527
Fragments arranged by size:
5527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6532
Fragments arranged by size:
6532 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3227 1186
Size: 2041 4716
BseYI C'CCAG_C
Cuts at: 2454 2966 5960 6456 2454
Size: 512 2994 496 2755
Fragments arranged by size:
2994 2755 512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3182 1163
Size: 573 1446 4738
Fragments arranged by size:
4738 1446 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3333 3475 3779 4207 4968 5996
Size: 275 186 1332 142 304 428 761 1028
Cuts at: 5996 1540
Size: 2301
Fragments arranged by size:
2301 1332 1028 761 428 304 275 186
142
BsiHKAI G_wGCw'C
Cuts at: 4818 4903 5974 4818
Size: 85 1071 5601
Fragments arranged by size:
5601 1071 85
BsmI GAATG_Cn'
Cuts at: 2360 4268 4361 2360
Size: 1908 93 4756
Fragments arranged by size:
4756 1908 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3752 4076 4818 4903 5974 1391
Size: 399 1962 324 742 85 1071 2174
Fragments arranged by size:
2174 1962 1071 742 399 324 85
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 3388 4646 6376 3388
Size: 1258 1730 3769
Fragments arranged by size:
3769 1730 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2605 4112 844
Size: 1761 1507 3489
Fragments arranged by size:
3489 1761 1507
BsrBI CCG'CTC
Cuts at: 1394 1823 4644 1394
Size: 429 2821 3507
Fragments arranged by size:
3507 2821 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2365 5252 5426 65
Size: 1183 1117 2887 174 1396
Fragments arranged by size:
2887 1396 1183 1117 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2116 2146 3225 3516 5401 5588 1700
Size: 110 306 30 1079 291 1885 187 2869
Fragments arranged by size:
2869 1885 1079 306 291 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6757
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6757
BssSI C'ACGA_G
Cuts at: 3291 4817 5829 3291
Size: 1526 1012 4219
Fragments arranged by size:
4219 1526 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2744 3721 3944 794
Size: 1950 977 223 3607
Fragments arranged by size:
3607 1950 977 223
BstBI TT'CG_AA
Cuts at: 5561 5561
Size: 6757
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6757
BstYI r'GATC_y
Cuts at: 1972 2321 4087 4454 4839 4856 6308 6394 6406
Size: 349 1766 367 385 17 1452 86 12
Cuts at: 6406 6752 1972
Size: 346 1977
Fragments arranged by size:
1977 1766 1452 385 367 349 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2067 2067
Size: 6757
BtgI C'CryG_G
Cuts at: 513 1065 2278 513
Size: 552 1213 4992
Fragments arranged by size:
4992 1213 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6018
Fragments arranged by size:
6018 683 56
BtsI GCAGTG_nn'
Cuts at: 2383 3170 4273 5067 5087 2383
Size: 787 1103 794 20 4053
Fragments arranged by size:
4053 1103 794 787 20
ClaI AT'CG_AT
Cuts at: 1554 3693 1554
Size: 2139 4618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6722
DraI TTT'AAA
Cuts at: 4061 4061
Size: 6757
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5764 809
Size: 507 4448 1802
Fragments arranged by size:
4448 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 3223 3227
Size: 54 1425 50 275 18 271 1122 4
Cuts at: 3227 4204 5093 6722 8
Size: 977 889 1629 43
Fragments arranged by size:
1629 1425 1122 977 889 275 271 54
50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 4204 1537
Size: 275 2392 4090
Fragments arranged by size:
4090 2392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4687 1353
Size: 3334 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2264 5344 5718 5864 387
Size: 1064 138 474 201 3080 374 146 1280
Fragments arranged by size:
3080 1280 1064 474 374 201 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2346 2553 3324 4820 5417 6204
Size: 393 576 138 207 771 1496 597 787
Cuts at: 6204 6472 1239
Size: 268 1524
Fragments arranged by size:
1524 1496 787 771 597 576 393 268
207 138
EcoO109I rG'GnC_Cy
Cuts at: 2071 3748 2071
Size: 1677 5080
EcoRI G'AATT_C
Cuts at: 2547 4066 4444 2547
Size: 1519 378 4860
Fragments arranged by size:
4860 1519 378
EcoRV GAT'ATC
Cuts at: 2801 2801
Size: 6757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2877 3595 2877
Size: 718 6039
FseI GG_CCGG'CC
Cuts at: 3229 3229
Size: 6757
FspI TGC'GCA
Cuts at: 5263 5263
Size: 6757
HaeII r_GCGC'y
Cuts at: 1678 2153 5904 1678
Size: 475 3751 2531
Fragments arranged by size:
3751 2531 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2306 2317 2338 3249
Size: 32 135 32 713 21 11 21 911
Cuts at: 3249 3281 3388 3420 5398 5430 5472 5504 1373
Size: 32 107 32 1978 32 42 32 2626
Fragments arranged by size:
2626 1978 911 713 135 107 42 32
32 32 32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 3241 3319 3739 4101 4347
Size: 988 281 697 606 78 420 362 246
Cuts at: 4347 669
Size: 3079
Fragments arranged by size:
3079 988 697 606 420 362 281 246
78
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6757
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KasI G'GCGC_C
Cuts at: 2149 2149
Size: 6757
KpnI G_GTAC'C
Cuts at: 2729 4082 2729
Size: 1353 5404
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2161 2592 3519 5871 6055 1305
Size: 856 431 927 2352 184 2007
Fragments arranged by size:
2352 2007 927 856 431 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4935
Fragments arranged by size:
4935 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2679 3768 4715 5074 5233 518
Size: 648 1513 1089 947 359 159 2042
Fragments arranged by size:
2042 1513 1089 947 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 3727 4173 4850 5998 6243 1606
Size: 480 1641 446 677 1148 245 2120
Fragments arranged by size:
2120 1641 1148 677 480 446 245
NaeI GCC'GGC
Cuts at: 2148 3227 2148
Size: 1079 5678
NarI GG'CG_CC
Cuts at: 2150 2150
Size: 6757
NcoI C'CATG_G
Cuts at: 513 1065 2278 513
Size: 552 1213 4992
Fragments arranged by size:
4992 1213 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6757
NgoMIV G'CCGG_C
Cuts at: 2146 3225 2146
Size: 1079 5678
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6757
NotI GC'GGCC_GC
Cuts at: 4204 4204
Size: 6757
NsiI A_TGCA'T
Cuts at: 2231 3130 2231
Size: 899 5858
NspI r_CATG'y
Cuts at: 1132 1229 2603 2974 3785 4115 5660 1132
Size: 97 1374 371 811 330 1545 2229
Fragments arranged by size:
2229 1545 1374 811 371 330 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6757
PciI A'CATG_T
Cuts at: 2599 5656 2599
Size: 3057 3700
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6757
PfoI T'CCnGG_A
Cuts at: 2221 2221
Size: 6757
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3453 3485 4826 4858 6339 6371 3453
Size: 32 1341 32 1481 32 3839
Fragments arranged by size:
3839 1481 1341 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6757
PsiI TTA'TAA
Cuts at: 2607 4327 2607
Size: 1720 5037
PspOMI G'GGCC_C
Cuts at: 3748 3748
Size: 6757
PstI C_TGCA'G
Cuts at: 830 2390 3726 4109 830
Size: 1560 1336 383 3478
Fragments arranged by size:
3478 1560 1336 383
PvuI CG_AT'CG
Cuts at: 2001 3475 2001
Size: 1474 5283
PvuII CAG'CTG
Cuts at: 2086 3727 2086
Size: 1641 5116
SalI G'TCGA_C
Cuts at: 1936 4099 1936
Size: 2163 4594
SbfI CC_TGCA'GG
Cuts at: 4109 4109
Size: 6757
ScaI AGT'ACT
Cuts at: 5005 6717 5005
Size: 1712 5045
SfcI C'TryA_G
Cuts at: 826 1045 2386 2414 3637 3722 3990 4105 5240
Size: 219 1341 28 1223 85 268 115 1135
Cuts at: 5240 5921 6112 826
Size: 681 191 1471
Fragments arranged by size:
1471 1341 1223 1135 681 268 219 191
115 85 28
SfoI GGC'GCC
Cuts at: 2151 2151
Size: 6757
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 2146 1700
Size: 446 6311
SmaI CCC'GGG
Cuts at: 1872 2136 4084 1872
Size: 264 1948 4545
Fragments arranged by size:
4545 1948 264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3020 4861 5762 6024 820
Size: 197 926 1077 1841 901 262 1553
Fragments arranged by size:
1841 1553 1077 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4548 493
Size: 4055 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6757
SphI G_CATG'C
Cuts at: 1132 4115 1132
Size: 2983 3774
SspI AAT'ATT
Cuts at: 5 52 2656 4681 5
Size: 47 2604 2025 2081
Fragments arranged by size:
2604 2081 2025 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6757
StyI C'CwwG_G
Cuts at: 513 1065 2278 2469 4196 513
Size: 552 1213 191 1727 3074
Fragments arranged by size:
3074 1727 1213 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3370 3600 4784 4801 4954 5139 5584
Size: 1255 1790 230 1184 17 153 185 445
Cuts at: 5584 325
Size: 1498
Fragments arranged by size:
1790 1498 1255 1184 445 230 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 2885 2916
Size: 274 80 33 51 1443 183 725 31
Cuts at: 2916 5003 6715 96
Size: 2087 1712 138
Fragments arranged by size:
2087 1712 1443 725 274 183 138 80
51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2081 2113 6717 6749 2081
Size: 32 4604 32 2089
Fragments arranged by size:
4604 2089 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6757
XbaI T'CTAG_A
Cuts at: 4093 4093
Size: 6757
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6757
XmaI C'CCGG_G
Cuts at: 1870 2134 4082 1870
Size: 264 1948 4545
Fragments arranged by size:
4545 1948 264
XmnI GAAnn'nnTTC
Cuts at: 3465 4886 3465
Size: 1421 5336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4565 276
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FspAI NruI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI