Restriction map for
pFN21AE1175
(Circular) (Six-base) MAPSORT of: pFN21AE1175.seq Check: 7512 from: 1 to: 6697
pFN21AE1175 6697 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3392 3392
Size: 6697
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3819 4507 278
Size: 53 83 186 936 2283 688 2468
Fragments arranged by size:
2468 2283 936 688 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4040 1937
Size: 2103 4594
Acc65I G'GTAC_C
Cuts at: 4018 4018
Size: 6697
AclI AA'CG_TT
Cuts at: 1563 4824 5197 1563
Size: 3261 373 3063
Fragments arranged by size:
3261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2221 3969 4760 6144 6412 1632
Size: 589 1748 791 1384 268 1917
Fragments arranged by size:
1917 1748 1384 791 589 268
AflII C'TTAA_G
Cuts at: 820 1017 2174 820
Size: 197 1157 5343
Fragments arranged by size:
5343 1157 197
AflIII A'CryG_T
Cuts at: 1203 5596 6573 1203
Size: 4393 977 1327
Fragments arranged by size:
4393 1327 977
AgeI A'CCGG_T
Cuts at: 2548 5528 2548
Size: 2980 3717
AhdI GACnn_n'nnGTC
Cuts at: 5426 5426
Size: 6697
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3100 1184
Size: 1916 4781
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3094 3126 3094
Size: 32 6665
AlwNI CAG_nnn'CTG
Cuts at: 3248 4440 6012 3248
Size: 1192 1572 3933
Fragments arranged by size:
3933 1572 1192
ApaLI G'TGCA_C
Cuts at: 4754 5910 4754
Size: 1156 5541
ApoI r'AATT_y
Cuts at: 1493 2251 2270 2717 3554 4006 4232 4384 6493
Size: 758 19 447 837 452 226 152 2109
Cuts at: 6493 1493
Size: 1697
Fragments arranged by size:
2109 1697 837 758 452 447 226 152
19
AseI AT'TA_AT
Cuts at: 160 1032 5251 160
Size: 872 4219 1606
Fragments arranged by size:
4219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
AvaI C'yCGr_G
Cuts at: 1870 1943 4022 1870
Size: 73 2079 4545
Fragments arranged by size:
4545 2079 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2550 2583 2550
Size: 33 6664
BamHI G'GATC_C
Cuts at: 4027 4394 4027
Size: 367 6330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4018 5473 618
Size: 325 217 627 2231 1455 1842
Fragments arranged by size:
2231 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2583 4016 1391
Size: 1192 1433 4072
Fragments arranged by size:
4072 1433 1192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3039 928
Size: 971 1140 4586
Fragments arranged by size:
4586 1140 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4888 4922 1903
Size: 34 50 34 2867 34 3678
Fragments arranged by size:
3678 2867 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4589 5799 4589
Size: 1210 5487
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6697
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5308 136
Size: 107 122 71 631 605 3636 1525
Fragments arranged by size:
3636 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3980 6692 3980
Size: 2712 3985
BlpI GC'TnA_GC
Cuts at: 4114 4114
Size: 6697
Bme1580I G_kGCm'C
Cuts at: 1790 4758 5914 1790
Size: 2968 1156 2573
Fragments arranged by size:
2968 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6697
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4428 5386 442
Size: 416 53 83 399 24 3011 958 1753
Fragments arranged by size:
3011 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2103 2276 5357 1239
Size: 864 173 3081 2579
Fragments arranged by size:
3081 2579 864 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4822 5687 5985 4822
Size: 865 298 5534
Fragments arranged by size:
5534 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5360 882
Size: 617 3861 2219
Fragments arranged by size:
3861 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4488 493
Size: 711 3284 2702
Fragments arranged by size:
3284 2702 711
BsaBI GATnn'nnATC
Cuts at: 6691 6691
Size: 6697
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3816 4504 4886 5521
Size: 53 83 186 936 2283 688 382 635
Cuts at: 5521 275
Size: 1451
Fragments arranged by size:
2283 1451 936 688 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2548 5130 5528 5802 5949 6360 2548
Size: 2582 398 274 147 411 2885
Fragments arranged by size:
2885 2582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2274 2304 3265 3295 1178
Size: 30 165 30 871 30 961 30 4580
Fragments arranged by size:
4580 961 871 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6697
BseYI C'CCAG_C
Cuts at: 5900 6396 5900
Size: 496 6201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4147 4908 5936 1540
Size: 275 186 2146 761 1028 2301
Fragments arranged by size:
2301 2146 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4758 4843 5914 4758
Size: 85 1071 5541
Fragments arranged by size:
5541 1071 85
BsmI GAATG_Cn'
Cuts at: 4208 4301 4208
Size: 93 6604
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2583 4016 4758 4843 5914 1391
Size: 399 793 1433 742 85 1071 2174
Fragments arranged by size:
2174 1433 1071 793 742 399 85
BspEI T'CCGG_A
Cuts at: 6360 6360
Size: 6697
BspHI T'CATG_A
Cuts at: 4586 6316 4586
Size: 1730 4967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3392 4052 844
Size: 2548 660 3489
Fragments arranged by size:
3489 2548 660
BsrBI CCG'CTC
Cuts at: 1394 1823 4584 1394
Size: 429 2761 3507
Fragments arranged by size:
3507 2761 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5192 5366 65
Size: 1183 3944 174 1396
Fragments arranged by size:
3944 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2548 3334 5341 5528 1700
Size: 110 738 786 2007 187 2869
Fragments arranged by size:
2869 2007 786 738 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6697
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6697
BssSI C'ACGA_G
Cuts at: 4757 5769 4757
Size: 1012 5685
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6697
BstBI TT'CG_AA
Cuts at: 2752 5501 2752
Size: 2749 3948
BstEII G'GTnAC_C
Cuts at: 1184 2684 1184
Size: 1500 5197
BstYI r'GATC_y
Cuts at: 1972 2086 3980 4027 4394 4779 4796 6248 6334
Size: 114 1894 47 367 385 17 1452 86
Cuts at: 6334 6346 6692 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1894 1452 385 367 346 114 86
47 17 12
Bsu36I CC'TnA_GG
Cuts at: 2637 2637
Size: 6697
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5958
Fragments arranged by size:
5958 683 56
BtsI GCAGTG_nn'
Cuts at: 4213 5007 5027 4213
Size: 794 20 5883
Fragments arranged by size:
5883 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3853 3888 529
Size: 35 3289 35 3338
Fragments arranged by size:
3338 3289 35 35
DraI TTT'AAA
Cuts at: 4001 4001
Size: 6697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5704 809
Size: 507 4388 1802
Fragments arranged by size:
4388 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3406 4144 5033
Size: 54 1425 50 275 18 1576 738 889
Cuts at: 5033 6662 8
Size: 1629 43
Fragments arranged by size:
1629 1576 1425 889 738 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4144 1537
Size: 275 2332 4090
Fragments arranged by size:
4090 2332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3858 4627 1353
Size: 887 1618 769 3423
Fragments arranged by size:
3423 1618 887 769
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5284 5658 5804 387
Size: 1064 138 3695 374 146 1280
Fragments arranged by size:
3695 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2221 2276 3969 4760 5357 6144
Size: 393 471 118 55 1693 791 597 787
Cuts at: 6144 6412 1239
Size: 268 1524
Fragments arranged by size:
1693 1524 791 787 597 471 393 268
118 55
EcoO109I rG'GnC_Cy
Cuts at: 3191 3731 3191
Size: 540 6157
EcoRI G'AATT_C
Cuts at: 2717 4006 4384 2717
Size: 1289 378 5030
Fragments arranged by size:
5030 1289 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3750 3750
Size: 6697
FspI TGC'GCA
Cuts at: 5203 5203
Size: 6697
HaeII r_GCGC'y
Cuts at: 1678 5844 1678
Size: 4166 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2475 2686 2718 5338
Size: 32 135 32 871 32 211 32 2620
Cuts at: 5338 5370 5412 5444 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2620 871 211 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4041 4287 669
Size: 988 281 2103 246 3079
Fragments arranged by size:
3079 2103 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6697
HpaI GTT'AAC
Cuts at: 4287 4287
Size: 6697
KpnI G_GTAC'C
Cuts at: 4022 4022
Size: 6697
MfeI C'AATT_G
Cuts at: 4296 4296
Size: 6697
MluI A'CGCG_T
Cuts at: 6573 6573
Size: 6697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3572 5811 5995 1305
Size: 2267 2239 184 2007
Fragments arranged by size:
2267 2239 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3408 10
Size: 54 1425 343 1576 3299
Fragments arranged by size:
3299 1576 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2922 3082 3315 3531 4655 5014 5173
Size: 648 1756 160 233 216 1124 359 159
Cuts at: 5173 518
Size: 2042
Fragments arranged by size:
2042 1756 1124 648 359 233 216 160
159
MspA1I CmG'CkG
Cuts at: 1606 3440 4113 4790 5938 6183 1606
Size: 1834 673 677 1148 245 2120
Fragments arranged by size:
2120 1834 1148 677 673 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6145
NdeI CA'TA_TG
Cuts at: 387 3135 387
Size: 2748 3949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6697
NotI GC'GGCC_GC
Cuts at: 4144 4144
Size: 6697
NspI r_CATG'y
Cuts at: 1132 1229 3081 4055 5600 1132
Size: 97 1852 974 1545 2229
Fragments arranged by size:
2229 1852 1545 974 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6697
PciI A'CATG_T
Cuts at: 5596 5596
Size: 6697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6697
PfoI T'CCnGG_A
Cuts at: 2073 2997 2073
Size: 924 5773
PmeI GTTT'AAAC
Cuts at: 4001 4001
Size: 6697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 6279 6311 4766
Size: 32 1481 32 5152
Fragments arranged by size:
5152 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6697
PsiI TTA'TAA
Cuts at: 4267 4267
Size: 6697
PstI C_TGCA'G
Cuts at: 830 3403 4049 830
Size: 2573 646 3478
Fragments arranged by size:
3478 2573 646
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6697
PvuII CAG'CTG
Cuts at: 3440 3440
Size: 6697
SalI G'TCGA_C
Cuts at: 1936 4039 1936
Size: 2103 4594
SbfI CC_TGCA'GG
Cuts at: 4049 4049
Size: 6697
ScaI AGT'ACT
Cuts at: 2488 4945 6657 2488
Size: 2457 1712 2528
Fragments arranged by size:
2528 2457 1712
SfcI C'TryA_G
Cuts at: 826 1045 2111 2574 3249 3399 4045 5180 5861
Size: 219 1066 463 675 150 646 1135 681
Cuts at: 5861 6052 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1066 681 675 646 463 219
191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6697
SmaI CCC'GGG
Cuts at: 1872 4024 1872
Size: 2152 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 4801 5702 5964 820
Size: 197 926 231 2627 901 262 1553
Fragments arranged by size:
2627 1553 926 901 262 231 197
SnaBI TAC'GTA
Cuts at: 493 4488 493
Size: 3995 2702
SpeI A'CTAG_T
Cuts at: 152 3386 152
Size: 3234 3463
SphI G_CATG'C
Cuts at: 1132 3081 4055 1132
Size: 1949 974 3774
Fragments arranged by size:
3774 1949 974
SspI AAT'ATT
Cuts at: 5 52 4621 5
Size: 47 4569 2081
Fragments arranged by size:
4569 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6697
StyI C'CwwG_G
Cuts at: 513 1065 4136 513
Size: 552 3071 3074
Fragments arranged by size:
3074 3071 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4724 4741 4894 5079 5524 325
Size: 1255 3144 17 153 185 445 1498
Fragments arranged by size:
3144 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2486 2758 2790
Size: 274 80 33 51 1443 509 272 32
Cuts at: 2790 3974 4943 6655 96
Size: 1184 969 1712 138
Fragments arranged by size:
1712 1443 1184 969 509 274 272 138
80 51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 6657 6689 2274
Size: 32 4351 32 2282
Fragments arranged by size:
4351 2282 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6697
XbaI T'CTAG_A
Cuts at: 2726 4033 2726
Size: 1307 5390
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6697
XmaI C'CCGG_G
Cuts at: 1870 4022 1870
Size: 2152 4545
XmnI GAAnn'nnTTC
Cuts at: 4826 4826
Size: 6697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3817 4505 276
Size: 53 83 186 936 2283 688 2468
Fragments arranged by size:
2468 2283 936 688 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI