Restriction map for
pFN21AE1173
(Circular) (Six-base) MAPSORT of: pFN21AE1173.seq Check: 704 from: 1 to: 6523
pFN21AE1173 6523 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4333 278
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3866 1937
Size: 1929 4594
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 2679 3016 4586 5970 6238 1632
Size: 534 513 337 1570 1384 268 1917
Fragments arranged by size:
1917 1570 1384 534 513 337 268
AfeI AGC'GCT
Cuts at: 2363 2363
Size: 6523
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 2892 3792 5422 6399 1203
Size: 1689 900 1630 977 1327
Fragments arranged by size:
1689 1630 1327 977 900
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 3556 4266 5838 3556
Size: 710 1572 4241
Fragments arranged by size:
4241 1572 710
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 2108 2932 3331 3608 3832 4058 4210 6319
Size: 615 824 399 277 224 226 152 2109
Cuts at: 6319 1493
Size: 1697
Fragments arranged by size:
2109 1697 824 615 399 277 226 224
152
AseI AT'TA_AT
Cuts at: 160 1032 3198 5077 160
Size: 872 2166 1879 1606
Fragments arranged by size:
2166 1879 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 3257 3848 1870
Size: 73 1314 591 4545
Fragments arranged by size:
4545 1314 591 73
BamHI G'GATC_C
Cuts at: 2716 3853 4220 2716
Size: 1137 367 5019
Fragments arranged by size:
5019 1137 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3844 5299 618
Size: 325 217 627 2057 1455 1842
Fragments arranged by size:
2057 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2788 3842 1391
Size: 629 768 1054 4072
Fragments arranged by size:
4072 1054 768 629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2564 928
Size: 971 665 4887
Fragments arranged by size:
4887 971 665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2309 2343 4714 4748 1903
Size: 34 372 34 2371 34 3678
Fragments arranged by size:
3678 2371 372 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4415 5625 4415
Size: 1210 5313
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5134 136
Size: 107 122 71 631 605 3462 1525
Fragments arranged by size:
3462 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6518 6518
Size: 6523
BlpI GC'TnA_GC
Cuts at: 3940 3940
Size: 6523
Bme1580I G_kGCm'C
Cuts at: 1790 4584 5740 1790
Size: 2794 1156 2573
Fragments arranged by size:
2794 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6523
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4254 5212 442
Size: 416 53 83 399 24 2837 958 1753
Fragments arranged by size:
2837 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3440
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 3575 3731 5183 1239
Size: 918 1418 156 1452 2579
Fragments arranged by size:
2579 1452 1418 918 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2481 4648 5513 5811 2481
Size: 2167 865 298 3193
Fragments arranged by size:
3193 2167 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5186 882
Size: 617 3687 2219
Fragments arranged by size:
3687 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4314 493
Size: 711 3110 2702
Fragments arranged by size:
3110 2702 711
BsaBI GATnn'nnATC
Cuts at: 3267 6517 3267
Size: 3250 3273
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4330 4712 5347 275
Size: 53 83 186 936 2797 382 635 1451
Fragments arranged by size:
2797 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2898 4956 5354 5628 5775 6186 2898
Size: 2058 398 274 147 411 3235
Fragments arranged by size:
3235 2058 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6298
Fragments arranged by size:
6298 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2028 3614 1186
Size: 842 1586 4095
Fragments arranged by size:
4095 1586 842
BseYI C'CCAG_C
Cuts at: 2028 3549 5726 6222 2028
Size: 1521 2177 496 2329
Fragments arranged by size:
2329 2177 1521 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5950
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3973 4734 5762 1540
Size: 275 186 1972 761 1028 2301
Fragments arranged by size:
2301 1972 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4584 4669 5740 4584
Size: 85 1071 5367
Fragments arranged by size:
5367 1071 85
BsmI GAATG_Cn'
Cuts at: 2967 4034 4127 2967
Size: 1067 93 5363
Fragments arranged by size:
5363 1067 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2788 3842 4584 4669 5740 1391
Size: 399 230 768 1054 742 85 1071 2174
Fragments arranged by size:
2174 1071 1054 768 742 399 230 85
BspEI T'CCGG_A
Cuts at: 2898 6186 2898
Size: 3288 3235
BspHI T'CATG_A
Cuts at: 4412 6142 4412
Size: 1730 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3878 844
Size: 3034 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4410 1394
Size: 429 2587 3507
Fragments arranged by size:
3507 2587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2789 5018 5192 65
Size: 1183 1541 2229 174 1396
Fragments arranged by size:
2229 1541 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5167 5354 1700
Size: 110 3357 187 2869
Fragments arranged by size:
3357 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 3526 3787 5327 3526
Size: 261 1540 4722
Fragments arranged by size:
4722 1540 261
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6523
BstXI CCAn_nnnn'nTGG
Cuts at: 3599 3599
Size: 6523
BstYI r'GATC_y
Cuts at: 1972 2602 2716 3262 3853 4220 4605 4622 6074
Size: 630 114 546 591 367 385 17 1452
Cuts at: 6074 6160 6172 6518 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 630 591 546 385 367 346
114 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 513
Size: 552 939 32 5000
Fragments arranged by size:
5000 939 552 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5784
Fragments arranged by size:
5784 683 56
BtsI GCAGTG_nn'
Cuts at: 2267 3244 4039 4833 4853 2267
Size: 977 795 794 20 3937
Fragments arranged by size:
3937 977 795 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5530 809
Size: 507 4214 1802
Fragments arranged by size:
4214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3108 3970 4859
Size: 54 1425 50 275 18 1278 862 889
Cuts at: 4859 6488 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1278 889 862 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3431 4453 1353
Size: 2078 1022 3423
Fragments arranged by size:
3423 2078 1022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2729 5110 5484 5630 387
Size: 1064 138 1140 2381 374 146 1280
Fragments arranged by size:
2381 1280 1140 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2166 2679 3016 3575 3731 4586
Size: 393 525 9 513 337 559 156 855
Cuts at: 4586 5183 5970 6238 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 855 787 597 559 525 513 393
337 268 156 9
EcoO109I rG'GnC_Cy
Cuts at: 3149 3149
Size: 6523
EcoRI G'AATT_C
Cuts at: 3832 4210 3832
Size: 378 6145
EcoRV GAT'ATC
Cuts at: 2155 2155
Size: 6523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3539 3539
Size: 6523
FspI TGC'GCA
Cuts at: 3491 5029 3491
Size: 1538 4985
HaeII r_GCGC'y
Cuts at: 1678 2365 5670 1678
Size: 687 3305 2531
Fragments arranged by size:
3305 2531 687
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3195 3227 5164 5196 5238
Size: 32 135 32 1623 32 1937 32 42
Cuts at: 5238 5270 1373
Size: 32 2626
Fragments arranged by size:
2626 1937 1623 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2890 3867 4113 669
Size: 988 281 952 977 246 3079
Fragments arranged by size:
3079 988 977 952 281 246
HindIII A'AGCT_T
Cuts at: 748 3393 748
Size: 2645 3878
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 4122 4122
Size: 6523
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5637 5821 1305
Size: 4332 184 2007
Fragments arranged by size:
4332 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4701
Fragments arranged by size:
4701 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 3299 3659 4481 4840 4999 518
Size: 648 1644 489 360 822 359 159 2042
Fragments arranged by size:
2042 1644 822 648 489 360 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2975 3939 4616 5764 6009 1606
Size: 432 937 964 677 1148 245 2120
Fragments arranged by size:
2120 1148 964 937 677 432 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5032
Fragments arranged by size:
5032 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 2896 3490 3881 5426 1132
Size: 97 1667 594 391 1545 2229
Fragments arranged by size:
2229 1667 1545 594 391 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6523
PciI A'CATG_T
Cuts at: 2892 5422 2892
Size: 2530 3993
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2249 2307 1108
Size: 1141 58 5324
Fragments arranged by size:
5324 1141 58
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 6105 6137 4592
Size: 32 1481 32 4978
Fragments arranged by size:
4978 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3149 3149
Size: 6523
PshAI GACnn'nnGTC
Cuts at: 1613 2821 1613
Size: 1208 5315
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PstI C_TGCA'G
Cuts at: 830 3875 830
Size: 3045 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2975 2975
Size: 6523
SacII CC_GC'GG
Cuts at: 2039 2039
Size: 6523
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 4771 6483 4771
Size: 1712 4811
SfcI C'TryA_G
Cuts at: 826 1045 3378 3871 5006 5687 5878 826
Size: 219 2333 493 1135 681 191 1471
Fragments arranged by size:
2333 1471 1135 681 493 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3259 3850 1872
Size: 1387 591 4545
Fragments arranged by size:
4545 1387 591
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2496 4627 5528 5790 820
Size: 197 926 553 2131 901 262 1553
Fragments arranged by size:
2131 1553 926 901 553 262 197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 3358 152
Size: 3206 3317
SphI G_CATG'C
Cuts at: 1132 3490 3881 1132
Size: 2358 391 3774
Fragments arranged by size:
3774 2358 391
SspI AAT'ATT
Cuts at: 5 52 4447 5
Size: 47 4395 2081
Fragments arranged by size:
4395 2081 47
StuI AGG'CCT
Cuts at: 1517 3498 1517
Size: 1981 4542
StyI C'CwwG_G
Cuts at: 513 1065 2004 3716 3962 513
Size: 552 939 1712 246 3074
Fragments arranged by size:
3074 1712 939 552 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4550 4567 4720 4905 5350 325
Size: 1255 2970 17 153 185 445 1498
Fragments arranged by size:
2970 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3169 4769 6481
Size: 274 80 33 51 1443 1192 1600 1712
Cuts at: 6481 96
Size: 138
Fragments arranged by size:
1712 1600 1443 1192 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6483 6515 6483
Size: 32 6491
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6523
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 3257 3848 1870
Size: 1387 591 4545
Fragments arranged by size:
4545 1387 591
XmnI GAAnn'nnTTC
Cuts at: 3522 3612 4652 3522
Size: 90 1040 5393
Fragments arranged by size:
5393 1040 90
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4331 276
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI