Restriction map for
pFN21AE1172
(Circular) (Six-base) MAPSORT of: pFN21AE1172.seq Check: 3680 from: 1 to: 6589
pFN21AE1172 6589 bp
With 182 enzymes: SgfI *
March 28, 2012 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3932 1937
Size: 1995 4594
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2737 3409 3493 3829 4652 6036 6304 1632
Size: 1105 672 84 336 823 1384 268 1917
Fragments arranged by size:
1917 1384 1105 823 672 336 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 5488 6465 1203
Size: 4285 977 1327
Fragments arranged by size:
4285 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 2123
Size: 32 6557
AlwNI CAG_nnn'CTG
Cuts at: 4332 5904 4332
Size: 1572 5017
ApaLI G'TGCA_C
Cuts at: 4646 5802 4646
Size: 1156 5433
ApoI r'AATT_y
Cuts at: 1493 2030 2388 2454 2787 2871 2955 3207 3459
Size: 537 358 66 333 84 84 252 252
Cuts at: 3459 3543 3795 3898 4124 4276 6385 1493
Size: 84 252 103 226 152 2109 1697
Fragments arranged by size:
2109 1697 537 358 333 252 252 252
226 152 103 84 84 84 66
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 3914 1870
Size: 73 1971 4545
Fragments arranged by size:
4545 1971 73
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3910 5365 618
Size: 325 217 627 2123 1455 1842
Fragments arranged by size:
2123 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2285 3908 1391
Size: 894 1623 4072
Fragments arranged by size:
4072 1623 894
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 2911 3415 3429 3499 3765 3835
Size: 971 248 764 504 14 70 266 70
Cuts at: 3835 928
Size: 3682
Fragments arranged by size:
3682 971 764 504 266 248 70 70
14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 2506 1548
Size: 958 5631
BfrBI ATG'CAT
Cuts at: 2707 2707
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5200 136
Size: 107 122 71 631 605 3528 1525
Fragments arranged by size:
3528 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3252 3756 6584 3252
Size: 504 2828 3257
Fragments arranged by size:
3257 2828 504
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 4650 5806 1790
Size: 2860 1156 2573
Fragments arranged by size:
2860 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4320 5278 442
Size: 416 53 83 399 24 2903 958 1753
Fragments arranged by size:
2903 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2733 2817 2901 2985 3489 3657 5249 1239
Size: 1494 84 84 84 504 168 1592 2579
Fragments arranged by size:
2579 1592 1494 504 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4714 5579 5877 4714
Size: 865 298 5426
Fragments arranged by size:
5426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2143 3217 5252 882
Size: 617 644 1074 2035 2219
Fragments arranged by size:
2219 2035 1074 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 4380 493
Size: 711 3176 2702
Fragments arranged by size:
3176 2702 711
BsaBI GATnn'nnATC
Cuts at: 6583 6583
Size: 6589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4396 4778 5413 275
Size: 53 83 186 936 2863 382 635 1451
Fragments arranged by size:
2863 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5022 5420 5694 5841 6252 5022
Size: 398 274 147 411 5359
Fragments arranged by size:
5359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 1178
Size: 30 165 30 720 30 5614
Fragments arranged by size:
5614 720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 1186
Size: 1107 5482
BseYI C'CCAG_C
Cuts at: 5792 6288 5792
Size: 496 6093
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3853 1163
Size: 573 2117 3899
Fragments arranged by size:
3899 2117 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4039 4800 5828 1540
Size: 275 186 2038 761 1028 2301
Fragments arranged by size:
2301 2038 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2285 2577 4650 4735 5806 2285
Size: 292 2073 85 1071 3068
Fragments arranged by size:
3068 2073 1071 292 85
BsmI GAATG_Cn'
Cuts at: 2709 4100 4193 2709
Size: 1391 93 5105
Fragments arranged by size:
5105 1391 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2577 3908 4650 4735 5806 1391
Size: 399 495 292 1331 742 85 1071 2174
Fragments arranged by size:
2174 1331 1071 742 495 399 292 85
BspEI T'CCGG_A
Cuts at: 6252 6252
Size: 6589
BspHI T'CATG_A
Cuts at: 2734 2818 2902 3733 4478 6208 2734
Size: 84 84 831 745 1730 3115
Fragments arranged by size:
3115 1730 831 745 84 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3944 844
Size: 3100 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4476 1394
Size: 429 2653 3507
Fragments arranged by size:
3507 2653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5084 5258 65
Size: 1183 3836 174 1396
Fragments arranged by size:
3836 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5233 5420 1700
Size: 110 3423 187 2869
Fragments arranged by size:
3423 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2253 3831 794
Size: 1459 1578 3552
Fragments arranged by size:
3552 1578 1459
BstBI TT'CG_AA
Cuts at: 2839 5393 2839
Size: 2554 4035
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstYI r'GATC_y
Cuts at: 1972 2197 3252 3756 3919 4286 4671 4688 6140
Size: 225 1055 504 163 367 385 17 1452
Cuts at: 6140 6226 6238 6584 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1055 504 385 367 346 225
163 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5098
Fragments arranged by size:
5098 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 2656 4105 4899 4919 2656
Size: 1449 794 20 4326
Fragments arranged by size:
4326 1449 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 2484 3893 2484
Size: 1409 5180
DraIII CAC_nnn'GTG
Cuts at: 3831 3831
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5596 809
Size: 507 4280 1802
Fragments arranged by size:
4280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2578 4036
Size: 54 1425 50 275 18 213 535 1458
Cuts at: 4036 4925 6554 8
Size: 889 1629 43
Fragments arranged by size:
1629 1458 1425 889 535 275 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4036 1537
Size: 275 2224 4090
Fragments arranged by size:
4090 2224 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2214 2756 4519 1353
Size: 705 156 542 1763 3423
Fragments arranged by size:
3423 1763 705 542 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5176 5550 5696 387
Size: 1064 138 3587 374 146 1280
Fragments arranged by size:
3587 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2283 2283
Size: 6589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2733 2737 2817 2901 2985 3409 3489
Size: 393 1101 4 80 84 84 424 80
Cuts at: 3489 3493 3657 3829 4652 5249 6036 6304 1239
Size: 4 164 172 823 597 787 268 1524
Fragments arranged by size:
1524 1101 823 787 597 424 393 268
172 164 84 84 80 80 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2015
Size: 6589
EcoO109I rG'GnC_Cy
Cuts at: 2021 2021
Size: 6589
EcoRI G'AATT_C
Cuts at: 2787 2871 3207 3459 3795 3898 4276 2787
Size: 84 336 252 336 103 378 5100
Fragments arranged by size:
5100 378 336 336 252 103 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3159 3159
Size: 6589
FspI TGC'GCA
Cuts at: 2088 5095 2088
Size: 3007 3582
HaeII r_GCGC'y
Cuts at: 1678 5736 1678
Size: 4058 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 3184 3216 3244
Size: 32 135 32 845 32 735 32 28
Cuts at: 3244 3276 3748 3780 5230 5262 5304 5336 1373
Size: 32 472 32 1450 32 42 32 2626
Fragments arranged by size:
2626 1450 845 735 472 135 42 32
32 32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2556 2684 2852 748
Size: 1808 128 168 4485
Fragments arranged by size:
4485 1808 168 128
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 5703 5887 1305
Size: 792 3606 184 2007
Fragments arranged by size:
3606 2007 792 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 10
Size: 54 1425 343 213 4554
Fragments arranged by size:
4554 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2355 2739 2823 2907 3048 3300
Size: 648 873 316 384 84 84 141 252
Cuts at: 3300 4547 4906 5065 518
Size: 1247 359 159 2042
Fragments arranged by size:
2042 1247 873 648 384 359 316 252
159 141 84 84
MspA1I CmG'CkG
Cuts at: 1606 4005 4682 5830 6075 1606
Size: 2399 677 1148 245 2120
Fragments arranged by size:
2399 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5098
Fragments arranged by size:
5098 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NsiI A_TGCA'T
Cuts at: 2709 2709
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3947 5492 1132
Size: 97 2718 1545 2229
Fragments arranged by size:
2718 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2143 1348
Size: 795 5794
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6589
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 4658 4690 6171 6203 2123
Size: 32 2503 32 1481 32 2509
Fragments arranged by size:
2509 2503 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2021 2021
Size: 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 2562 4159 2562
Size: 1597 4992
PstI C_TGCA'G
Cuts at: 830 2214 3941 830
Size: 1384 1727 3478
Fragments arranged by size:
3478 1727 1384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
SacI G_AGCT'C
Cuts at: 2285 2285
Size: 6589
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SanDI GG'GwC_CC
Cuts at: 2021 2021
Size: 6589
SapI GCTCTTCn'nnn_
Cuts at: 2756 2756
Size: 6589
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SexAI A'CCwGG_T
Cuts at: 2133 3525 2133
Size: 1392 5197
SfcI C'TryA_G
Cuts at: 826 1045 2210 2363 3937 5072 5753 5944 826
Size: 219 1165 153 1574 1135 681 191 1471
Fragments arranged by size:
1574 1471 1165 1135 681 219 191 153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4693 5594 5856 820
Size: 197 926 2750 901 262 1553
Fragments arranged by size:
2750 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 3646 3693 1517
Size: 2129 47 4413
Fragments arranged by size:
4413 2129 47
StyI C'CwwG_G
Cuts at: 513 1065 2004 4028 513
Size: 552 939 2024 3074
Fragments arranged by size:
3074 2024 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2385 4616 4633 4786 4971 5416 325
Size: 1255 805 2231 17 153 185 445 1498
Fragments arranged by size:
2231 1498 1255 805 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4835 6547 96
Size: 274 80 33 51 1443 2858 1712 138
Fragments arranged by size:
2858 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6549 6581 6549
Size: 32 6557
Tth111I GACn'n_nGTC
Cuts at: 1316 2831 2915 3167 1316
Size: 1515 84 252 4738
Fragments arranged by size:
4738 1515 252 84
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 2845 4718 2845
Size: 1873 4716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SfiI
SfoI SrfI SwaI XcmI