Restriction map for
pFN21AE1164
(Circular) (Six-base) MAPSORT of: pFN21AE1164.seq Check: 992 from: 1 to: 6484
pFN21AE1164 6484 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2788 3827 1937
Size: 851 1039 4594
Fragments arranged by size:
4594 1039 851
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3120 4547 5931 6199 1632
Size: 1488 1427 1384 268 1917
Fragments arranged by size:
1917 1488 1427 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2501 820
Size: 197 1484 4803
Fragments arranged by size:
4803 1484 197
AflIII A'CryG_T
Cuts at: 1203 3452 5383 6360 1203
Size: 2249 1931 977 1327
Fragments arranged by size:
2249 1931 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2836 1184
Size: 1652 4832
AlwNI CAG_nnn'CTG
Cuts at: 4227 5799 4227
Size: 1572 4912
ApaLI G'TGCA_C
Cuts at: 2496 4541 5697 2496
Size: 2045 1156 3283
Fragments arranged by size:
3283 2045 1156
ApoI r'AATT_y
Cuts at: 1493 2138 3045 3116 3793 4019 4171 6280 1493
Size: 645 907 71 677 226 152 2109 1697
Fragments arranged by size:
2109 1697 907 677 645 226 152 71
AseI AT'TA_AT
Cuts at: 160 1032 2892 5038 160
Size: 872 1860 2146 1606
Fragments arranged by size:
2146 1860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3809 1870
Size: 73 1866 4545
Fragments arranged by size:
4545 1866 73
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3805 5260 618
Size: 325 217 627 2018 1455 1842
Fragments arranged by size:
2018 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3803 1391
Size: 2412 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 3503 928
Size: 971 354 1250 3909
Fragments arranged by size:
3909 1250 971 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5095 136
Size: 107 122 71 631 605 3423 1525
Fragments arranged by size:
3423 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3464 6479 3464
Size: 3015 3469
BlpI GC'TnA_GC
Cuts at: 3258 3901 3258
Size: 643 5841
Bme1580I G_kGCm'C
Cuts at: 1790 2500 4545 5701 1790
Size: 710 2045 1156 2573
Fragments arranged by size:
2573 2045 1156 710
BmgBI CAC'GTC
Cuts at: 1318 3722 1318
Size: 2404 4080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2911 4215 5173
Size: 416 53 83 399 24 1494 1304 958
Cuts at: 5173 442
Size: 1753
Fragments arranged by size:
1753 1494 1304 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 2940 2987 3424 3512 5144 1239
Size: 1413 288 47 437 88 1632 2579
Fragments arranged by size:
2579 1632 1413 437 288 88 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 4609 5474 5772 2894
Size: 1715 865 298 3606
Fragments arranged by size:
3606 1715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2055 5147 882
Size: 617 556 3092 2219
Fragments arranged by size:
3092 2219 617 556
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4291 4673 5308 275
Size: 53 83 186 936 2758 382 635 1451
Fragments arranged by size:
2758 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6259
Fragments arranged by size:
6259 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6484
BseYI C'CCAG_C
Cuts at: 5687 6183 5687
Size: 496 5988
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3324 3767 1163
Size: 573 1588 443 3880
Fragments arranged by size:
3880 1588 573 443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3934 4695 5723 1540
Size: 275 186 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2500 3526 4545 4630 5701 2500
Size: 1026 1019 85 1071 3283
Fragments arranged by size:
3283 1071 1026 1019 85
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2500 3526 3803 4545 4630 5701 1391
Size: 399 710 1026 277 742 85 1071 2174
Fragments arranged by size:
2174 1071 1026 742 710 399 277 85
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 2535 2683 3112 4373 6103 2535
Size: 148 429 1261 1730 2916
Fragments arranged by size:
2916 1730 1261 429 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2015 2573 3671 3839 844
Size: 1171 558 1098 168 3489
Fragments arranged by size:
3489 1171 1098 558 168
BsrBI CCG'CTC
Cuts at: 1394 1823 4371 1394
Size: 429 2548 3507
Fragments arranged by size:
3507 2548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2525 2705 3181 4979 5153 65
Size: 1183 1277 180 476 1798 174 1396
Fragments arranged by size:
1798 1396 1277 1183 476 180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5128 5315 1700
Size: 110 3318 187 2869
Fragments arranged by size:
3318 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2318 96
Size: 2222 4262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2799 2359
Size: 440 6044
BstYI r'GATC_y
Cuts at: 1972 3464 3814 4181 4566 4583 6035 6121 6133
Size: 1492 350 367 385 17 1452 86 12
Cuts at: 6133 6479 1972
Size: 346 1977
Fragments arranged by size:
1977 1492 1452 385 367 350 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5932
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2612 2681 4000 4794 4814 2468
Size: 144 69 1319 794 20 4138
Fragments arranged by size:
4138 1319 794 144 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3121 3788 3121
Size: 667 5817
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2095 5491 809
Size: 507 779 3396 1802
Fragments arranged by size:
3396 1802 779 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 3931 4820
Size: 54 1425 50 275 18 550 1551 889
Cuts at: 4820 6449 8
Size: 1629 43
Fragments arranged by size:
1629 1551 1425 889 550 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2328 3496 3514 4414 1353
Size: 786 189 1168 18 900 3423
Fragments arranged by size:
3423 1168 900 786 189 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5071 5445 5591 387
Size: 1064 138 3482 374 146 1280
Fragments arranged by size:
3482 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2652 2940 2987 3120 3424 3512 4547
Size: 393 1020 288 47 133 304 88 1035
Cuts at: 4547 5144 5931 6199 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1035 1020 787 597 393 304 288
268 133 88 47
EcoO109I rG'GnC_Cy
Cuts at: 2627 2627
Size: 6484
EcoRI G'AATT_C
Cuts at: 3045 3793 4171 3045
Size: 748 378 5358
Fragments arranged by size:
5358 748 378
EcoRV GAT'ATC
Cuts at: 2390 2390
Size: 6484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 6484
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 5631 1678
Size: 3953 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3263 3295 5125 5157 5199
Size: 32 135 32 1691 32 1830 32 42
Cuts at: 5199 5231 1373
Size: 32 2626
Fragments arranged by size:
2626 1830 1691 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 3452 6360 3452
Size: 2908 3576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5598 5782 1305
Size: 4293 184 2007
Fragments arranged by size:
4293 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2382 10
Size: 54 1425 343 550 4112
Fragments arranged by size:
4112 1425 550 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2797 2818 2996 4442 4801 4960 518
Size: 648 1631 21 178 1446 359 159 2042
Fragments arranged by size:
2042 1631 1446 648 359 178 159 21
MspA1I CmG'CkG
Cuts at: 1606 3900 4577 5725 5970 1606
Size: 2294 677 1148 245 2120
Fragments arranged by size:
2294 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5932
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 2136 2963 3696 3842 5387 1132
Size: 97 907 827 733 146 1545 2229
Fragments arranged by size:
2229 1545 907 827 733 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PstI C_TGCA'G
Cuts at: 830 2762 3682 3836 830
Size: 1932 920 154 3478
Fragments arranged by size:
3478 1932 920 154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SapI GCTCTTCn'nnn_
Cuts at: 3514 3514
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 2016 2554 2654 2758 3207 3318 3678
Size: 219 971 538 100 104 449 111 360
Cuts at: 3678 3832 4967 5648 5839 826
Size: 154 1135 681 191 1471
Fragments arranged by size:
1471 1135 971 681 538 449 360 219
191 154 111 104 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 2873 4588 5489 5751 820
Size: 197 926 558 372 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 558 372 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6484
StyI C'CwwG_G
Cuts at: 513 1065 2383 3923 513
Size: 552 1318 1540 3074
Fragments arranged by size:
3074 1540 1318 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4511 4528 4681 4866 5311 325
Size: 1255 2931 17 153 185 445 1498
Fragments arranged by size:
2931 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2318 4730 6442
Size: 274 80 33 51 1443 341 2412 1712
Cuts at: 6442 96
Size: 138
Fragments arranged by size:
2412 1712 1443 341 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6444 6476 6444
Size: 32 6452
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI