Restriction map for
pFN21AE1163
(Circular) (Six-base) MAPSORT of: pFN21AE1163.seq Check: 4096 from: 1 to: 6445
pFN21AE1163 6445 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3153 4255 278
Size: 53 83 186 936 1617 1102 2468
Fragments arranged by size:
2468 1617 1102 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 1937
Size: 1851 4594
Acc65I G'GTAC_C
Cuts at: 3766 3766
Size: 6445
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2589 2884 4508 5892 6160 1632
Size: 555 402 295 1624 1384 268 1917
Fragments arranged by size:
1917 1624 1384 555 402 295 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 2013 2960 5344 6321 1203
Size: 810 947 2384 977 1327
Fragments arranged by size:
2384 1327 977 947 810
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 3118 1166
Size: 1952 4493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2388 1184
Size: 1204 5241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 2684
Size: 32 6413
AlwNI CAG_nnn'CTG
Cuts at: 2213 2231 2519 2567 3496 4188 5760 2213
Size: 18 288 48 929 692 1572 2898
Fragments arranged by size:
2898 1572 929 692 288 48 18
ApaI G_GGCC'C
Cuts at: 2501 2815 2501
Size: 314 6131
ApaLI G'TGCA_C
Cuts at: 4502 5658 4502
Size: 1156 5289
ApoI r'AATT_y
Cuts at: 1493 2792 2825 3739 3754 3980 4132 6241 1493
Size: 1299 33 914 15 226 152 2109 1697
Fragments arranged by size:
2109 1697 1299 914 226 152 33 15
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2802 3770 1870
Size: 73 149 710 968 4545
Fragments arranged by size:
4545 968 710 149 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3139 3172 3139
Size: 33 6412
BamHI G'GATC_C
Cuts at: 3663 3775 4142 3663
Size: 112 367 5966
Fragments arranged by size:
5966 367 112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3766 5221 618
Size: 325 217 627 1979 1455 1842
Fragments arranged by size:
1979 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2501 2815 3764 1391
Size: 633 477 314 949 4072
Fragments arranged by size:
4072 949 633 477 314
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3386 928
Size: 971 1487 3987
Fragments arranged by size:
3987 1487 971
BbvCI CC'TCA_GC
Cuts at: 3519 3519
Size: 6445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4636 4670 1903
Size: 34 2699 34 3678
Fragments arranged by size:
3678 2699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2968 4337 5547 2968
Size: 1369 1210 3866
Fragments arranged by size:
3866 1369 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5056 136
Size: 107 122 71 631 605 3384 1525
Fragments arranged by size:
3384 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6440 6440
Size: 6445
BlpI GC'TnA_GC
Cuts at: 3862 3862
Size: 6445
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2501 2815 4506 5662 1790
Size: 310 401 314 1691 1156 2573
Fragments arranged by size:
2573 1691 1156 401 314 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2150 4176 5134
Size: 416 53 83 399 24 733 2026 958
Cuts at: 5134 442
Size: 1753
Fragments arranged by size:
2026 1753 958 733 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2693 5105 1239
Size: 1454 2412 2579
Fragments arranged by size:
2579 2412 1454
Bpu10I CC'TnA_GC
Cuts at: 2187 2379 3519 2187
Size: 192 1140 5113
Fragments arranged by size:
5113 1140 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2325 4570 5435 5733 2325
Size: 2245 865 298 3037
Fragments arranged by size:
3037 2245 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2411 5108 882
Size: 617 912 2697 2219
Fragments arranged by size:
2697 2219 912 617
BsaAI yAC'GTr
Cuts at: 493 1204 2016 4236 493
Size: 711 812 2220 2702
Fragments arranged by size:
2702 2220 812 711
BsaBI GATnn'nnATC
Cuts at: 2424 2667 2874 6439 2424
Size: 243 207 3565 2430
Fragments arranged by size:
3565 2430 243 207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3150 4252 4634 5269
Size: 53 83 186 936 1617 1102 382 635
Cuts at: 5269 275
Size: 1451
Fragments arranged by size:
1617 1451 1102 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4878 5276 5550 5697 6108 4878
Size: 398 274 147 411 5215
Fragments arranged by size:
5215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3316 3346 1178
Size: 30 165 30 1913 30 4277
Fragments arranged by size:
4277 1913 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2864 1186
Size: 1678 4767
BseYI C'CCAG_C
Cuts at: 2192 5648 6144 2192
Size: 3456 496 2493
Fragments arranged by size:
3456 2493 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 2562 2879 3117 1163
Size: 573 415 411 317 238 4491
Fragments arranged by size:
4491 573 415 411 317 238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 4506 4591 5662 2024
Size: 2482 85 1071 2807
Fragments arranged by size:
2807 2482 1071 85
BsmI GAATG_Cn'
Cuts at: 3956 4049 3956
Size: 93 6352
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2100 2501 2815 3764 4506 4591
Size: 399 234 76 401 314 949 742 85
Cuts at: 4591 5662 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 949 742 401 399 314 234
85 76
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 4334 6064 4334
Size: 1730 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 3800 844
Size: 2143 813 3489
Fragments arranged by size:
3489 2143 813
BsrBI CCG'CTC
Cuts at: 1394 1823 4332 1394
Size: 429 2509 3507
Fragments arranged by size:
3507 2509 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2279 4940 5114 65
Size: 1183 1031 2661 174 1396
Fragments arranged by size:
2661 1396 1183 1031 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5089 5276 1700
Size: 110 3279 187 2869
Fragments arranged by size:
3279 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6445
BssSI C'ACGA_G
Cuts at: 4505 5517 4505
Size: 1012 5433
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6445
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6445
BstXI CCAn_nnnn'nTGG
Cuts at: 3120 3319 3659 3120
Size: 199 340 5906
Fragments arranged by size:
5906 340 199
BstYI r'GATC_y
Cuts at: 1972 2104 2227 2739 3123 3663 3775 4142 4527
Size: 132 123 512 384 540 112 367 385
Cuts at: 4527 4544 5996 6082 6094 6440 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 540 512 385 384 367 346
132 123 112 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3312 3436 513
Size: 552 2247 124 3522
Fragments arranged by size:
3522 2247 552 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5706
Fragments arranged by size:
5706 683 56
BtsI GCAGTG_nn'
Cuts at: 3448 3961 4755 4775 3448
Size: 513 794 20 5118
Fragments arranged by size:
5118 794 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DraIII CAC_nnn'GTG
Cuts at: 3077 3622 3077
Size: 545 5900
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5452 809
Size: 507 4136 1802
Fragments arranged by size:
4136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3892 4781 6410
Size: 54 1425 50 275 18 2062 889 1629
Cuts at: 6410 8
Size: 43
Fragments arranged by size:
2062 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2608 3113 3293 4375 1353
Size: 1255 505 180 1082 3423
Fragments arranged by size:
3423 1255 1082 505 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 5032 5406 5552 387
Size: 1064 138 506 2937 374 146 1280
Fragments arranged by size:
2937 1280 1064 506 374 146 138
EcoICRI GAG'CTC
Cuts at: 2022 2022
Size: 6445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2589 2693 2884 4508 5105 5892
Size: 393 555 402 104 191 1624 597 787
Cuts at: 5892 6160 1239
Size: 268 1524
Fragments arranged by size:
1624 1524 787 597 555 402 393 268
191 104
EcoO109I rG'GnC_Cy
Cuts at: 2497 2498 3025 3225 2497
Size: 1 527 200 5717
Fragments arranged by size:
5717 527 200 1
EcoRI G'AATT_C
Cuts at: 2825 3754 4132 2825
Size: 929 378 5138
Fragments arranged by size:
5138 929 378
EcoRV GAT'ATC
Cuts at: 3136 3136
Size: 6445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 6445
FspI TGC'GCA
Cuts at: 3248 4951 3248
Size: 1703 4742
HaeII r_GCGC'y
Cuts at: 1678 5592 1678
Size: 3914 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 2895 2927 3316
Size: 32 135 32 710 32 581 32 389
Cuts at: 3316 3348 5086 5118 5160 5192 1373
Size: 32 1738 32 42 32 2626
Fragments arranged by size:
2626 1738 710 581 389 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KpnI G_GTAC'C
Cuts at: 3770 3770
Size: 6445
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2159 3061 5559 5743 1305
Size: 684 170 902 2498 184 2007
Fragments arranged by size:
2498 2007 902 684 184 170
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4623
Fragments arranged by size:
4623 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2925 3118 4403 4762 4921 518
Size: 648 1759 193 1285 359 159 2042
Fragments arranged by size:
2042 1759 1285 648 359 193 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2074 3861 4538 5686 5931 1606
Size: 441 27 1787 677 1148 245 2120
Fragments arranged by size:
2120 1787 1148 677 441 245 27
NcoI C'CATG_G
Cuts at: 513 1065 3312 3436 513
Size: 552 2247 124 3522
Fragments arranged by size:
3522 2247 552 124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 2654 2964 3803 5348 1132
Size: 97 1425 310 839 1545 2229
Fragments arranged by size:
2229 1545 1425 839 310 97
PasI CC'CwG_GG
Cuts at: 1348 2501 3195 1348
Size: 1153 694 4598
Fragments arranged by size:
4598 1153 694
PciI A'CATG_T
Cuts at: 2960 5344 2960
Size: 2384 4061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3496 1108
Size: 2388 4057
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PmlI CAC'GTG
Cuts at: 2016 2016
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 6027 6059 4514
Size: 32 1481 32 4900
Fragments arranged by size:
4900 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3225 3225
Size: 6445
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PspOMI G'GGCC_C
Cuts at: 2497 2811 2497
Size: 314 6131
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3507 3539 3507
Size: 32 6413
PstI C_TGCA'G
Cuts at: 830 2073 2367 3797 830
Size: 1243 294 1430 3478
Fragments arranged by size:
3478 1430 1243 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2047 2047
Size: 6445
SacI G_AGCT'C
Cuts at: 2024 2024
Size: 6445
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SanDI GG'GwC_CC
Cuts at: 3225 3225
Size: 6445
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 4693 6405 4693
Size: 1712 4733
SfcI C'TryA_G
Cuts at: 826 1045 2069 2223 2363 2559 3269 3793 4928
Size: 219 1024 154 140 196 710 524 1135
Cuts at: 4928 5609 5800 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1024 710 681 524 219 196
191 154 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 3772 1872
Size: 1900 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2340 2802 4549 5450 5712 820
Size: 197 926 397 462 1747 901 262 1553
Fragments arranged by size:
1747 1553 926 901 462 397 262 197
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6445
StyI C'CwwG_G
Cuts at: 513 1065 2769 2844 3312 3436 3478 3643 3884
Size: 552 1704 75 468 124 42 165 241
Cuts at: 3884 513
Size: 3074
Fragments arranged by size:
3074 1704 552 468 241 165 124 75
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2185 2465 3117 3364 4472 4489 4642
Size: 1255 605 280 652 247 1108 17 153
Cuts at: 4642 4827 5272 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1108 652 605 445 280 247
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4691 6403 96
Size: 274 80 33 51 1443 2714 1712 138
Fragments arranged by size:
2714 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3115 3147 6405 6437 3115
Size: 32 3258 32 3123
Fragments arranged by size:
3258 3123 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2883 1316
Size: 1567 4878
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XhoI C'TCGA_G
Cuts at: 1943 2802 1943
Size: 859 5586
XmaI C'CCGG_G
Cuts at: 1870 3770 1870
Size: 1900 4545
XmnI GAAnn'nnTTC
Cuts at: 2793 4574 2793
Size: 1781 4664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3151 4253 276
Size: 53 83 186 936 1617 1102 2468
Fragments arranged by size:
2468 1617 1102 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI