Restriction map for
pFN21AE1160
(Circular) (Six-base) MAPSORT of: pFN21AE1160.seq Check: 4051 from: 1 to: 6286
pFN21AE1160 6286 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3594 4349 5733 6001 1632
Size: 1962 755 1384 268 1917
Fragments arranged by size:
1962 1917 1384 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2097 2910 3132 3390 5185 6162 1203
Size: 894 813 222 258 1795 977 1327
Fragments arranged by size:
1795 1327 977 894 813 258 222
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 3025 5015 3025
Size: 1990 4296
AleI CACnn'nnGTG
Cuts at: 1166 3131 1166
Size: 1965 4321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2498 1184
Size: 1314 4972
AlwNI CAG_nnn'CTG
Cuts at: 2017 2077 2086 2256 2299 2401 4029 5601 2017
Size: 60 9 170 43 102 1628 1572 2702
Fragments arranged by size:
2702 1628 1572 170 102 60 43 9
ApaI G_GGCC'C
Cuts at: 2772 2772
Size: 6286
ApaLI G'TGCA_C
Cuts at: 3394 4343 5499 3394
Size: 949 1156 4181
Fragments arranged by size:
4181 1156 949
ApoI r'AATT_y
Cuts at: 1493 3595 3821 3973 6082 1493
Size: 2102 226 152 2109 1697
Fragments arranged by size:
2109 2102 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 2380 2987 3611 1870
Size: 73 437 607 624 4545
Fragments arranged by size:
4545 624 607 437 73
BamHI G'GATC_C
Cuts at: 3616 3983 3616
Size: 367 5919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2351 2798 3125 3254 3607
Size: 325 217 627 564 447 327 129 353
Cuts at: 3607 5062 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 564 447 353 327 325
217 129
BanII G_rGCy'C
Cuts at: 1391 2378 2433 2441 2772 3605 1391
Size: 987 55 8 331 833 4072
Fragments arranged by size:
4072 987 833 331 55 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2510 928
Size: 971 611 4704
Fragments arranged by size:
4704 971 611
BbvCI CC'TCA_GC
Cuts at: 2249 3456 2249
Size: 1207 5079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3356 3390 4477 4511 1903
Size: 34 1419 34 1087 34 3678
Fragments arranged by size:
3678 1419 1087 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 2138 3383
Size: 107 122 71 631 605 457 9 1245
Cuts at: 3383 3552 4897 136
Size: 169 1345 1525
Fragments arranged by size:
1525 1345 1245 631 605 457 169 122
107 71 9
BglII A'GATC_T
Cuts at: 2901 6281 2901
Size: 3380 2906
BlpI GC'TnA_GC
Cuts at: 2273 3703 2273
Size: 1430 4856
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2772 3398 4347 5503 1790
Size: 564 418 626 949 1156 2573
Fragments arranged by size:
2573 1156 949 626 564 418
BmgBI CAC'GTC
Cuts at: 1318 2098 2778 3169 1318
Size: 780 680 391 4435
Fragments arranged by size:
4435 780 680 391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2224 4017 4975
Size: 416 53 83 399 24 807 1793 958
Cuts at: 4975 442
Size: 1753
Fragments arranged by size:
1793 1753 958 807 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 3131 4946 1239
Size: 836 1056 1815 2579
Fragments arranged by size:
2579 1815 1056 836
Bpu10I CC'TnA_GC
Cuts at: 2249 3456 2249
Size: 1207 5079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2788 3434 4411 5276 5574 2351
Size: 437 646 977 865 298 3063
Fragments arranged by size:
3063 977 865 646 437 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3530 4949 882
Size: 617 2031 1419 2219
Fragments arranged by size:
2219 2031 1419 617
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 2027 6280 2027
Size: 4253 2033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3026 3465 4093 4475
Size: 53 83 186 936 1493 439 628 382
Cuts at: 4475 5110 275
Size: 635 1451
Fragments arranged by size:
1493 1451 936 635 628 439 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2680 3228 4719 5117 5391 5538 5949 2680
Size: 548 1491 398 274 147 411 3017
Fragments arranged by size:
3017 1491 548 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2861 2870 2873 2888 1186
Size: 1240 435 9 3 15 4584
Fragments arranged by size:
4584 1240 435 15 9 3
BseYI C'CCAG_C
Cuts at: 2582 2697 2771 5489 5985 2582
Size: 115 74 2718 496 2883
Fragments arranged by size:
2883 2718 496 115 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 2552 1163
Size: 573 507 309 4897
Fragments arranged by size:
4897 573 507 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3387 3736 4497 5525 1540
Size: 275 186 1386 349 761 1028 2301
Fragments arranged by size:
2301 1386 1028 761 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2378 3315 3398 3450 4347 4432 5503 2378
Size: 937 83 52 897 85 1071 3161
Fragments arranged by size:
3161 1071 937 897 85 83 52
BsmI GAATG_Cn'
Cuts at: 3379 3797 3890 3379
Size: 418 93 5775
Fragments arranged by size:
5775 418 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3033 3033
Size: 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2354 2378 2433 2441 2772 3315 3398
Size: 399 564 24 55 8 331 543 83
Cuts at: 3398 3450 3605 4347 4432 5503 1391
Size: 52 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 564 543 399 331 155
85 83 55 52 24 8
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2968 3574 3641 844
Size: 2124 606 67 3489
Fragments arranged by size:
3489 2124 606 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2785 3267 4173 1394
Size: 429 962 482 906 3507
Fragments arranged by size:
3507 962 906 482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2602 4781 4955 65
Size: 1183 1354 2179 174 1396
Fragments arranged by size:
2179 1396 1354 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 2268 2646 2732 2895 3257 4930
Size: 110 402 56 378 86 163 362 1673
Cuts at: 4930 5117 1700
Size: 187 2869
Fragments arranged by size:
2869 1673 402 378 362 187 163 110
86 56
BsrGI T'GTAC_A
Cuts at: 96 2307 2913 2922 3135 96
Size: 2211 606 9 213 3247
Fragments arranged by size:
3247 2211 606 213 9
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 2489 3548 4346 5358 2489
Size: 1059 798 1012 3417
Fragments arranged by size:
3417 1059 1012 798
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 794
Size: 1745 4541
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2028 2244 2328 2901 3616 3983 4368 4385
Size: 56 216 84 573 715 367 385 17
Cuts at: 4385 5837 5923 5935 6281 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 715 573 385 367 346 216
86 84 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2199 2442 2544 2706 513
Size: 552 939 195 243 102 162 4093
Fragments arranged by size:
4093 939 552 243 195 162 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3364 487
Size: 683 56 2138 3409
Fragments arranged by size:
3409 2138 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2540 3802 4596 4616 2015
Size: 525 1262 794 20 3685
Fragments arranged by size:
3685 1262 794 525 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2462 2547 2569
Size: 54 1425 50 275 18 632 85 22
Cuts at: 2569 2613 2941 3240 3384 3733 4622 6251 8
Size: 44 328 299 144 349 889 1629 43
Fragments arranged by size:
1629 1425 889 632 349 328 299 275
144 85 54 50 44 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3384 3733 1537
Size: 275 1572 349 4090
Fragments arranged by size:
4090 1572 349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3274 4216 1353
Size: 1921 942 3423
Fragments arranged by size:
3423 1921 942
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2376 2376
Size: 6286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 3131 3594 4349 4946 5733 6001
Size: 393 443 1056 463 755 597 787 268
Cuts at: 6001 1239
Size: 1524
Fragments arranged by size:
1524 1056 787 755 597 463 443 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2083 2173 2398 2416 2965 3193 3563 2083
Size: 90 225 18 549 228 370 4806
Fragments arranged by size:
4806 549 370 228 225 90 18
EcoRI G'AATT_C
Cuts at: 3595 3973 3595
Size: 378 5908
FspI TGC'GCA
Cuts at: 3419 4792 3419
Size: 1373 4913
HaeII r_GCGC'y
Cuts at: 1678 2199 2811 5433 1678
Size: 521 612 2622 2531
Fragments arranged by size:
2622 2531 612 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2405 2437 3011 3043 4927
Size: 32 135 32 833 32 574 32 1884
Cuts at: 4927 4959 5001 5033 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1884 833 574 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2406 2502 2760 3269 5400 5584 1305
Size: 1101 96 258 509 2131 184 2007
Fragments arranged by size:
2131 2007 1101 509 258 184 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2464 2549 2571 2615 3242
Size: 54 1425 343 632 85 22 44 627
Cuts at: 3242 10
Size: 3054
Fragments arranged by size:
3054 1425 632 627 343 85 54 44
22
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 2543 3131 4244 4603 4762 518
Size: 648 1116 261 588 1113 359 159 2042
Fragments arranged by size:
2042 1116 1113 648 588 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2482 3066 3154 3249 3702 4379 5527
Size: 468 408 584 88 95 453 677 1148
Cuts at: 5527 5772 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 584 468 453 408 245
95 88
NaeI GCC'GGC
Cuts at: 2214 2270 2214
Size: 56 6230
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4795
Fragments arranged by size:
4795 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NgoMIV G'CCGG_C
Cuts at: 2212 2268 2212
Size: 56 6230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2408 2542 2914 3394 3644 5189 1132
Size: 97 1179 134 372 480 250 1545 2229
Fragments arranged by size:
2229 1545 1179 480 372 250 134 97
PasI CC'CwG_GG
Cuts at: 1348 2395 1348
Size: 1047 5239
PciI A'CATG_T
Cuts at: 2910 3390 5185 2910
Size: 480 1795 4011
Fragments arranged by size:
4011 1795 480
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2735 1108
Size: 1627 4659
PfoI T'CCnGG_A
Cuts at: 2052 2169 2331 2970 2052
Size: 117 162 639 5368
Fragments arranged by size:
5368 639 162 117
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 3193 3563 2173
Size: 1020 370 4896
Fragments arranged by size:
4896 1020 370
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PspOMI G'GGCC_C
Cuts at: 2768 2768
Size: 6286
PstI C_TGCA'G
Cuts at: 830 2022 2082 2328 3585 3638 830
Size: 1192 60 246 1257 53 3478
Fragments arranged by size:
3478 1257 1192 246 60 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 3154 3249 3154
Size: 95 6191
SacI G_AGCT'C
Cuts at: 2378 2378
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SanDI GG'GwC_CC
Cuts at: 3563 3563
Size: 6286
SapI GCTCTTCn'nnn_
Cuts at: 3274 3274
Size: 6286
SbfI CC_TGCA'GG
Cuts at: 3585 3638 3585
Size: 53 6233
ScaI AGT'ACT
Cuts at: 3329 4534 6246 3329
Size: 1205 1712 3369
Fragments arranged by size:
3369 1712 1205
SfcI C'TryA_G
Cuts at: 826 1045 2018 2078 2324 2470 3581 3634 4769
Size: 219 973 60 246 146 1111 53 1135
Cuts at: 4769 5450 5641 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1111 973 681 246 219 191
146 60 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2138 2138
Size: 6286
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 2803 3449 4390 5291 5553
Size: 197 926 423 437 646 941 901 262
Cuts at: 5553 820
Size: 1553
Fragments arranged by size:
1553 941 926 901 646 437 423 262
197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 2408 2542 3644 1132
Size: 1276 134 1102 3774
Fragments arranged by size:
3774 1276 1102 134
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 2004 3435 3725 513
Size: 552 939 1431 290 3074
Fragments arranged by size:
3074 1431 939 552 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 4313 4330 4483 4668 5113 325
Size: 1255 1926 807 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 2307 2913
Size: 274 80 33 51 1443 111 219 606
Cuts at: 2913 2922 3135 3327 4532 6244 96
Size: 9 213 192 1205 1712 138
Fragments arranged by size:
1712 1443 1205 606 274 219 213 192
138 111 80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2632 2579
Size: 53 6233
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 4415 4415
Size: 6286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI