Restriction map for
pFN21AE1156
(Circular) (Six-base) MAPSORT of: pFN21AE1156.seq Check: 3465 from: 1 to: 6097
pFN21AE1156 6097 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 3440 1937
Size: 130 1373 4594
Fragments arranged by size:
4594 1373 130
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4160 5544 5812 1632
Size: 2528 1384 268 1917
Fragments arranged by size:
2528 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2147 1184
Size: 963 5134
AlwNI CAG_nnn'CTG
Cuts at: 2991 3840 5412 2991
Size: 849 1572 3676
Fragments arranged by size:
3676 1572 849
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2915 2999 3097 3167 3265 3406 3632 3784
Size: 1422 84 98 70 98 141 226 152
Cuts at: 3784 5893 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1422 226 152 141 98 98
84 70
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 3422 1870
Size: 73 1479 4545
Fragments arranged by size:
4545 1479 73
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3418 4873 618
Size: 325 217 627 1631 1455 1842
Fragments arranged by size:
1842 1631 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2435 3416 1391
Size: 1044 981 4072
Fragments arranged by size:
4072 1044 981
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5126
BbvCI CC'TCA_GC
Cuts at: 2187 2187
Size: 6097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 2373 1548
Size: 825 5272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4708 136
Size: 107 122 71 631 605 337 2699 1525
Fragments arranged by size:
2699 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 4158 5314 1790
Size: 2368 1156 2573
Fragments arranged by size:
2573 2368 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2294 3828 4786
Size: 416 53 83 399 24 877 1534 958
Cuts at: 4786 442
Size: 1753
Fragments arranged by size:
1753 1534 958 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2649 2733 2817 2833 2901 2985 3069
Size: 917 493 84 84 16 68 84 84
Cuts at: 3069 3153 3321 3337 3405 4757 1239
Size: 84 168 16 68 1352 2579
Fragments arranged by size:
2579 1352 917 493 168 84 84 84
84 84 68 68 16 16
Bpu10I CC'TnA_GC
Cuts at: 2034 2187 2199 2380 2034
Size: 153 12 181 5751
Fragments arranged by size:
5751 181 153 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2375 3001 4222 5087 5385 2375
Size: 626 1221 865 298 3087
Fragments arranged by size:
3087 1221 865 626 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5872
Fragments arranged by size:
5872 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2204 2323 1186
Size: 919 99 119 4960
Fragments arranged by size:
4960 919 119 99
BseYI C'CCAG_C
Cuts at: 2543 5300 5796 2543
Size: 2757 496 2844
Fragments arranged by size:
2844 2757 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2836 2920 3004 3172 3340 1163
Size: 573 1100 84 84 168 168 3920
Fragments arranged by size:
3920 1100 573 168 168 84 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3547 4308 5336 1540
Size: 275 186 15 1531 761 1028 2301
Fragments arranged by size:
2301 1531 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2435 4158 4243 5314 2435
Size: 1723 85 1071 3218
Fragments arranged by size:
3218 1723 1071 85
BsmI GAATG_Cn'
Cuts at: 2567 3608 3701 2567
Size: 1041 93 4963
Fragments arranged by size:
4963 1041 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2435 3416 4158 4243 5314 1391
Size: 399 645 981 742 85 1071 2174
Fragments arranged by size:
2174 1071 981 742 645 399 85
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 2856 3986 5716 2856
Size: 1130 1730 3237
Fragments arranged by size:
3237 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3385 3452 844
Size: 2541 67 3489
Fragments arranged by size:
3489 2541 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2033 3984 1394
Size: 429 210 1951 3507
Fragments arranged by size:
3507 1951 429 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 2131 3076 3244 4592 4766 65
Size: 1183 883 945 168 1348 174 1396
Fragments arranged by size:
1396 1348 1183 945 883 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 4741 4928 1700
Size: 110 2931 187 2869
Fragments arranged by size:
2931 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092
Size: 1455 367 385 17 1452 86 12 346
Cuts at: 6092 1972
Size: 1977
Fragments arranged by size:
1977 1455 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2021 2021
Size: 6097
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 2488 2572 2740 2824 2908 3613 4407 4427 2488
Size: 84 168 84 84 705 794 20 4158
Fragments arranged by size:
4158 794 705 168 84 84 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3058 3093 3226 3261 529
Size: 35 2494 35 133 35 3365
Fragments arranged by size:
3365 2494 133 35 35 35
DraI TTT'AAA
Cuts at: 3364 3401 3364
Size: 37 6060
DraIII CAC_nnn'GTG
Cuts at: 2401 2401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 3544 4433
Size: 54 1425 50 275 18 183 1531 889
Cuts at: 4433 6062 8
Size: 1629 43
Fragments arranged by size:
1629 1531 1425 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 3544 1537
Size: 275 201 1531 4090
Fragments arranged by size:
4090 1531 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 4027 1353
Size: 2674 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2433 2433
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2649 2733 2817 2833 2901 2985
Size: 393 524 493 84 84 16 68 84
Cuts at: 2985 3069 3153 3321 3337 3405 4160 4757 5544
Size: 84 84 168 16 68 755 597 787
Cuts at: 5544 5812 1239
Size: 268 1524
Fragments arranged by size:
1524 787 755 597 524 493 393 268
168 84 84 84 84 84 68 68
16 16
EcoO109I rG'GnC_Cy
Cuts at: 2026 2296 2365 2026
Size: 270 69 5758
Fragments arranged by size:
5758 270 69
EcoRI G'AATT_C
Cuts at: 3097 3265 3406 3784 3097
Size: 168 141 378 5410
Fragments arranged by size:
5410 378 168 141
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373
Size: 32 135 32 3166 32 42 32 2626
Fragments arranged by size:
3166 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2748 748
Size: 2000 4097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2622 2958 5211 5395 1305
Size: 1317 336 2253 184 2007
Fragments arranged by size:
2253 2007 1317 336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4275
Fragments arranged by size:
4275 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2952 4055 4414 4573 518
Size: 648 1786 1103 359 159 2042
Fragments arranged by size:
2042 1786 1103 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3513 4190 5338 5583 1606
Size: 1907 677 1148 245 2120
Fragments arranged by size:
2120 1907 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 2013 3544 2013
Size: 1531 4566
NspI r_CATG'y
Cuts at: 1132 1229 2543 2733 3455 5000 1132
Size: 97 1314 190 722 1545 2229
Fragments arranged by size:
2229 1545 1314 722 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2296 2026
Size: 270 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 2895 3667 2895
Size: 772 5325
PstI C_TGCA'G
Cuts at: 830 3449 830
Size: 2619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
SacI G_AGCT'C
Cuts at: 2435 2435
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SanDI GG'GwC_CC
Cuts at: 2026 2296 2026
Size: 270 5827
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SexAI A'CCwGG_T
Cuts at: 2397 2397
Size: 6097
SfcI C'TryA_G
Cuts at: 826 1045 2214 2920 3445 4580 5261 5452 826
Size: 219 1169 706 525 1135 681 191 1471
Fragments arranged by size:
1471 1169 1135 706 681 525 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2390 3016 4201 5102 5364 820
Size: 197 926 447 626 1185 901 262 1553
Fragments arranged by size:
1553 1185 926 901 626 447 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 2513 4021 5
Size: 47 2461 1508 2081
Fragments arranged by size:
2461 2081 1508 47
StuI AGG'CCT
Cuts at: 1517 2450 2638 2806 2974 1517
Size: 933 188 168 168 4640
Fragments arranged by size:
4640 933 188 168 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 3536 513
Size: 552 939 1532 3074
Fragments arranged by size:
3074 1532 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4124 4141 4294 4479 4924 325
Size: 1255 2544 17 153 185 445 1498
Fragments arranged by size:
2544 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI