Restriction map for
pFN21AE1155
(Circular) (Six-base) MAPSORT of: pFN21AE1155.seq Check: 7849 from: 1 to: 6223
pFN21AE1155 6223 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2043 3566 1937
Size: 106 1523 4594
Fragments arranged by size:
4594 1523 106
Acc65I G'GTAC_C
Cuts at: 3544 3544
Size: 6223
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2268 2364 4286 5670 5938 1632
Size: 636 96 1922 1384 268 1917
Fragments arranged by size:
1922 1917 1384 636 268 96
AfeI AGC'GCT
Cuts at: 2862 2862
Size: 6223
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 2115 2181 5122 6099 1203
Size: 912 66 2941 977 1327
Fragments arranged by size:
2941 1327 977 912 66
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2912 1184
Size: 1728 4495
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 6191
AlwNI CAG_nnn'CTG
Cuts at: 2291 3208 3966 5538 2291
Size: 917 758 1572 2976
Fragments arranged by size:
2976 1572 917 758
ApaLI G'TGCA_C
Cuts at: 4280 5436 4280
Size: 1156 5067
ApoI r'AATT_y
Cuts at: 1493 2165 3510 3532 3758 3910 6019 1493
Size: 672 1345 22 226 152 2109 1697
Fragments arranged by size:
2109 1697 1345 672 226 152 22
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 3548 1870
Size: 73 1605 4545
Fragments arranged by size:
4545 1605 73
BamHI G'GATC_C
Cuts at: 3553 3920 3553
Size: 367 5856
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3086 3544 4999 618
Size: 325 217 627 1299 458 1455 1842
Fragments arranged by size:
1842 1455 1299 627 458 325 217
BanII G_rGCy'C
Cuts at: 1391 2619 3467 3542 1391
Size: 1228 848 75 4072
Fragments arranged by size:
4072 1228 848 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3289 3426 928
Size: 971 1390 137 3725
Fragments arranged by size:
3725 1390 971 137
BbvCI CC'TCA_GC
Cuts at: 2903 2903
Size: 6223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2847 2881 4414 4448 1903
Size: 34 910 34 1533 34 3678
Fragments arranged by size:
3678 1533 910 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4115 5325 4115
Size: 1210 5013
BclI T'GATC_A
Cuts at: 1548 2551 1548
Size: 1003 5220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3266 4834 136
Size: 107 122 71 631 605 1594 1568 1525
Fragments arranged by size:
1594 1568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6218 6218
Size: 6223
BlpI GC'TnA_GC
Cuts at: 3640 3640
Size: 6223
Bme1580I G_kGCm'C
Cuts at: 1790 4284 5440 1790
Size: 2494 1156 2573
Fragments arranged by size:
2573 2494 1156
BmgBI CAC'GTC
Cuts at: 1318 2587 3424 1318
Size: 1269 837 4117
Fragments arranged by size:
4117 1269 837
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2421 3954 4912
Size: 416 53 83 399 24 1004 1533 958
Cuts at: 4912 442
Size: 1753
Fragments arranged by size:
1753 1533 1004 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3481 4883 1239
Size: 2242 1402 2579
Fragments arranged by size:
2579 2242 1402
Bpu10I CC'TnA_GC
Cuts at: 2903 2903
Size: 6223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3434 4348 5213 5511 3434
Size: 914 865 298 4146
Fragments arranged by size:
4146 914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4886 882
Size: 617 3387 2219
Fragments arranged by size:
3387 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2118 4014 493
Size: 711 914 1896 2702
Fragments arranged by size:
2702 1896 914 711
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2484 3146 3213 4030
Size: 53 83 186 936 951 662 67 817
Cuts at: 4030 4412 5047 275
Size: 382 635 1451
Fragments arranged by size:
1451 951 936 817 662 635 382 186
83 67 53
BsaWI w'CCGG_w
Cuts at: 4656 5054 5328 5475 5886 4656
Size: 398 274 147 411 4993
Fragments arranged by size:
4993 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2883 2913 1178
Size: 30 165 30 1480 30 4488
Fragments arranged by size:
4488 1480 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2891 3175 3389 3457 1186
Size: 979 726 284 214 68 3952
Fragments arranged by size:
3952 979 726 284 214 68
BseYI C'CCAG_C
Cuts at: 5426 5922 5426
Size: 496 5727
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2636 1163
Size: 573 426 474 4750
Fragments arranged by size:
4750 573 474 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 3673 4434 5462 1540
Size: 275 186 973 699 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 699 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2133 2990 3467 4284 4369 5440 2108
Size: 25 857 477 817 85 1071 2891
Fragments arranged by size:
2891 1071 857 817 477 85 25
BsmI GAATG_Cn'
Cuts at: 3443 3734 3827 3443
Size: 291 93 5839
Fragments arranged by size:
5839 291 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2133 2619 2990 3467 3542 4284
Size: 399 318 25 486 371 477 75 742
Cuts at: 4284 4369 5440 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 477 399 371 318
85 75 25
BspEI T'CCGG_A
Cuts at: 5886 5886
Size: 6223
BspHI T'CATG_A
Cuts at: 2841 4112 5842 2841
Size: 1271 1730 3222
Fragments arranged by size:
3222 1730 1271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2658 3283 3507 3578 844
Size: 1814 625 224 71 3489
Fragments arranged by size:
3489 1814 625 224 71
BsrBI CCG'CTC
Cuts at: 1394 1823 4110 1394
Size: 429 2287 3507
Fragments arranged by size:
3507 2287 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4718 4892 65
Size: 1183 3470 174 1396
Fragments arranged by size:
3470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2486 2958 2973 3070 4867 5054 1700
Size: 110 676 472 15 97 1797 187 2869
Fragments arranged by size:
2869 1797 676 472 187 110 97 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 3389 4283 5295 3389
Size: 894 1012 4317
Fragments arranged by size:
4317 1012 894
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2443 2794 794
Size: 1649 351 4223
Fragments arranged by size:
4223 1649 351
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6223
BstXI CCAn_nnnn'nTGG
Cuts at: 2534 2534
Size: 6223
BstYI r'GATC_y
Cuts at: 1972 2287 3204 3553 3920 4305 4322 5774 5860
Size: 315 917 349 367 385 17 1452 86
Cuts at: 5860 5872 6218 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 917 385 367 349 346 315
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3363 513
Size: 552 939 1359 3373
Fragments arranged by size:
3373 1359 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5484
Fragments arranged by size:
5484 683 56
BtsI GCAGTG_nn'
Cuts at: 2916 3364 3739 4533 4553 2916
Size: 448 375 794 20 4586
Fragments arranged by size:
4586 794 448 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 3527 3527
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5230 809
Size: 507 3914 1802
Fragments arranged by size:
3914 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 2956 3057
Size: 54 1425 50 275 18 513 613 101
Cuts at: 3057 3222 3670 4559 6188 8
Size: 165 448 889 1629 43
Fragments arranged by size:
1629 1425 889 613 513 448 275 165
101 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2383 2660 4153 1353
Size: 1030 277 1493 3423
Fragments arranged by size:
3423 1493 1030 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2621 2714 3152 3262 4810 5184
Size: 1064 138 1032 93 438 110 1548 374
Cuts at: 5184 5330 387
Size: 146 1280
Fragments arranged by size:
1548 1280 1064 1032 438 374 146 138
110 93
EcoICRI GAG'CTC
Cuts at: 3465 3465
Size: 6223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2268 2364 3481 4286 4883 5670 5938
Size: 393 636 96 1117 805 597 787 268
Cuts at: 5938 1239
Size: 1524
Fragments arranged by size:
1524 1117 805 787 636 597 393 268
96
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 3274 2024
Size: 1250 4973
EcoO109I rG'GnC_Cy
Cuts at: 2017 2307 2523 2017
Size: 290 216 5717
Fragments arranged by size:
5717 290 216
EcoRI G'AATT_C
Cuts at: 2165 3532 3910 2165
Size: 1367 378 4478
Fragments arranged by size:
4478 1367 378
FseI GG_CCGG'CC
Cuts at: 3074 3074
Size: 6223
FspI TGC'GCA
Cuts at: 4729 4729
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 2864 5370 1678
Size: 1186 2506 2531
Fragments arranged by size:
2531 2506 1186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2474 2506 2706
Size: 32 135 32 683 32 187 32 200
Cuts at: 2706 2738 3335 3367 4864 4896 4938 4970 1373
Size: 32 597 32 1497 32 42 32 2626
Fragments arranged by size:
2626 1497 683 597 200 187 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3567 3813 669
Size: 988 281 1629 246 3079
Fragments arranged by size:
3079 1629 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6223
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KpnI G_GTAC'C
Cuts at: 3548 3548
Size: 6223
MfeI C'AATT_G
Cuts at: 3822 3822
Size: 6223
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2539 5337 5521 1305
Size: 1234 2798 184 2007
Fragments arranged by size:
2798 2007 1234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2345 3059 3224 10
Size: 54 1425 343 513 714 165 3009
Fragments arranged by size:
3009 1425 714 513 343 165 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2762 4181 4540 4699 518
Size: 648 1596 1419 359 159 2042
Fragments arranged by size:
2042 1596 1419 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2931 3639 4316 5464 5709 1606
Size: 1325 708 677 1148 245 2120
Fragments arranged by size:
2120 1325 1148 708 677 245
NaeI GCC'GGC
Cuts at: 2488 3072 2488
Size: 584 5639
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4732
Fragments arranged by size:
4732 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6223
NgoMIV G'CCGG_C
Cuts at: 2486 3070 2486
Size: 584 5639
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6223
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 2185 3581 5126 1132
Size: 97 956 1396 1545 2229
Fragments arranged by size:
2229 1545 1396 956 97
PasI CC'CwG_GG
Cuts at: 1348 3076 1348
Size: 1728 4495
PciI A'CATG_T
Cuts at: 2181 5122 2181
Size: 2941 3282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3208 1108
Size: 2100 4123
PfoI T'CCnGG_A
Cuts at: 2290 2290
Size: 6223
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PmlI CAC'GTG
Cuts at: 2118 2118
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 4292 4324 5805 5837 3221
Size: 32 1039 32 1481 32 3607
Fragments arranged by size:
3607 1481 1039 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2017 2307 2523 2017
Size: 290 216 5717
Fragments arranged by size:
5717 290 216
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PstI C_TGCA'G
Cuts at: 830 2930 3275 3359 3504 3575 830
Size: 2100 345 84 145 71 3478
Fragments arranged by size:
3478 2100 345 145 84 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
SacI G_AGCT'C
Cuts at: 3467 3467
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SanDI GG'GwC_CC
Cuts at: 2523 2523
Size: 6223
SbfI CC_TGCA'GG
Cuts at: 3504 3575 3504
Size: 71 6152
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SfcI C'TryA_G
Cuts at: 826 1045 2926 3271 3355 3500 3571 4706 5387
Size: 219 1881 345 84 145 71 1135 681
Cuts at: 5387 5578 826
Size: 191 1471
Fragments arranged by size:
1881 1471 1135 681 345 219 191 145
84 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 3550 1872
Size: 1678 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3449 4327 5228 5490 820
Size: 197 926 1506 878 901 262 1553
Fragments arranged by size:
1553 1506 926 901 878 262 197
SnaBI TAC'GTA
Cuts at: 493 4014 493
Size: 3521 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 3026 3134 3269 3477 1517
Size: 1509 108 135 208 4263
Fragments arranged by size:
4263 1509 208 135 108
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 3662 513
Size: 552 939 183 1475 3074
Fragments arranged by size:
3074 1475 939 552 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 3074 4250 4267 4420 4605 5050
Size: 1255 984 510 1176 17 153 185 445
Cuts at: 5050 325
Size: 1498
Fragments arranged by size:
1498 1255 1176 984 510 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4469 6181 96
Size: 274 80 33 51 1443 2492 1712 138
Fragments arranged by size:
2492 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6183 6215 6183
Size: 32 6191
Tth111I GACn'n_nGTC
Cuts at: 1316 2407 2693 1316
Size: 1091 286 4846
Fragments arranged by size:
4846 1091 286
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6223
XmaI C'CCGG_G
Cuts at: 1870 3548 1870
Size: 1678 4545
XmnI GAAnn'nnTTC
Cuts at: 4352 4352
Size: 6223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PspOMI PspXI PsrI
PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI