Restriction map for
pFN21AE1151
(Circular) (Six-base) MAPSORT of: pFN21AE1151.seq Check: 6965 from: 1 to: 6163
pFN21AE1151 6163 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 3506 1937
Size: 229 1340 4594
Fragments arranged by size:
4594 1340 229
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2533 2711 2843 4226 5610 5878 1632
Size: 901 178 132 1383 1384 268 1917
Fragments arranged by size:
1917 1384 1383 901 268 178 132
AfeI AGC'GCT
Cuts at: 2546 2546
Size: 6163
AflII C'TTAA_G
Cuts at: 820 1017 2860 820
Size: 197 1843 4123
Fragments arranged by size:
4123 1843 197
AflIII A'CryG_T
Cuts at: 1203 5062 6039 1203
Size: 3859 977 1327
Fragments arranged by size:
3859 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 2109 2647 3906 5478 2109
Size: 538 1259 1572 2794
Fragments arranged by size:
2794 1572 1259 538
ApaI G_GGCC'C
Cuts at: 2192 2277 3154 2192
Size: 85 877 5201
Fragments arranged by size:
5201 877 85
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3267 3472 3698 3850 5959 1493
Size: 1774 205 226 152 2109 1697
Fragments arranged by size:
2109 1774 1697 226 205 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2203 2276 2369 2908 3488 1870
Size: 73 78 182 73 93 539 580 4545
Fragments arranged by size:
4545 580 539 182 93 78 73 73
BamHI G'GATC_C
Cuts at: 2890 3493 3860 2890
Size: 603 367 5193
Fragments arranged by size:
5193 603 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2049 2392 2721 3484
Size: 325 217 627 227 35 343 329 763
Cuts at: 3484 4939 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 763 627 343 329 325 227
217 35
BanII G_rGCy'C
Cuts at: 1391 2192 2277 2859 3154 3482 1391
Size: 801 85 582 295 328 4072
Fragments arranged by size:
4072 801 582 328 295 85
BbeI G_GCGC'C
Cuts at: 2018 2396 2018
Size: 378 5785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2831 3323 928
Size: 971 932 492 3768
Fragments arranged by size:
3768 971 932 492
BbvCI CC'TCA_GC
Cuts at: 2606 2606
Size: 6163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 3454 1548
Size: 1906 4257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4774 136
Size: 107 122 71 631 605 3102 1525
Fragments arranged by size:
3102 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3580 3580
Size: 6163
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2192 2277 3154 4224 5380 1790
Size: 264 138 85 877 1070 1156 2573
Fragments arranged by size:
2573 1156 1070 877 264 138 85
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3055 3894 4852
Size: 416 53 83 399 24 1638 839 958
Cuts at: 4852 442
Size: 1753
Fragments arranged by size:
1753 1638 958 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2636 2768 4823 1239
Size: 1178 219 132 2055 2579
Fragments arranged by size:
2579 2055 1178 219 132
Bpu10I CC'TnA_GC
Cuts at: 2312 2606 2768 2982 2312
Size: 294 162 214 5493
Fragments arranged by size:
5493 294 214 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3954 493
Size: 711 2750 2702
Fragments arranged by size:
2750 2702 711
BsaBI GATnn'nnATC
Cuts at: 3456 6157 3456
Size: 2701 3462
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2163 2361 2393
Size: 53 83 186 936 482 148 198 32
Cuts at: 2393 3970 4352 4987 275
Size: 1577 382 635 1451
Fragments arranged by size:
1577 1451 936 635 482 382 198 186
148 83 53 32
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5938
Fragments arranged by size:
5938 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2606 2639 2775 2863 3357 1186
Size: 1156 264 33 136 88 494 3992
Fragments arranged by size:
3992 1156 494 264 136 88 33
BseYI C'CCAG_C
Cuts at: 2257 2349 5366 5862 2257
Size: 92 3017 496 2558
Fragments arranged by size:
3017 2558 496 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2963 1163
Size: 573 1227 4363
Fragments arranged by size:
4363 1227 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2376 2859 4224 4309 5380 2376
Size: 483 1365 85 1071 3159
Fragments arranged by size:
3159 1365 1071 483 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2192 2277 2376 2859 3154 3482
Size: 399 264 138 85 99 483 295 328
Cuts at: 3482 4224 4309 5380 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 483 399 328 295 264
138 99 85 85
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2940 3518 844
Size: 2096 578 3489
Fragments arranged by size:
3489 2096 578
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 4050 1394
Size: 429 206 2021 3507
Fragments arranged by size:
3507 2021 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 2220 2363 2411 2664 3260 4807
Size: 110 270 140 143 48 253 596 1547
Cuts at: 4807 4994 1700
Size: 187 2869
Fragments arranged by size:
2869 1547 596 270 253 187 143 140
110 48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 2151 2292 1925
Size: 226 141 5796
Fragments arranged by size:
5796 226 141
BssSI C'ACGA_G
Cuts at: 4223 5235 4223
Size: 1012 5151
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2647 794
Size: 1853 4310
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2890 3293 3430 3493 3860 4245 4262 5714
Size: 918 403 137 63 367 385 17 1452
Cuts at: 5714 5800 5812 6158 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 918 403 385 367 346 137
86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2114 2114
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2004 2814 2898 2957 513
Size: 552 939 810 84 59 3719
Fragments arranged by size:
3719 939 810 552 84 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2302 487
Size: 683 56 1076 4348
Fragments arranged by size:
4348 1076 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 2944 3101 3679 4473 4493 2186
Size: 758 157 578 794 20 3856
Fragments arranged by size:
3856 794 758 578 157 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3610 4499
Size: 54 1425 50 275 18 268 1512 889
Cuts at: 4499 6128 8
Size: 1629 43
Fragments arranged by size:
1629 1512 1425 889 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3213 3326 4093 1353
Size: 1860 113 767 3423
Fragments arranged by size:
3423 1860 767 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2636 2717 4750 5124 5270 387
Size: 1064 138 1047 81 2033 374 146 1280
Fragments arranged by size:
2033 1280 1064 1047 374 146 138 81
EcoICRI GAG'CTC
Cuts at: 2857 2857
Size: 6163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2533 2636 2711 2768 2843 4226
Size: 393 785 116 103 75 57 75 1383
Cuts at: 4226 4823 5610 5878 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1383 787 785 597 393 268 116
103 75 75 57
EcoO109I rG'GnC_Cy
Cuts at: 2188 2189 2273 2308 2686 3151 2188
Size: 1 84 35 378 465 5200
Fragments arranged by size:
5200 465 378 84 35 1
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FspI TGC'GCA
Cuts at: 2936 4669 2936
Size: 1733 4430
HaeII r_GCGC'y
Cuts at: 1678 2018 2345 2396 2548 2731 5310 1678
Size: 340 327 51 152 183 2579 2531
Fragments arranged by size:
2579 2531 340 327 183 152 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3285 3317 4804 4836 4878
Size: 32 135 32 1713 32 1487 32 42
Cuts at: 4878 4910 1373
Size: 32 2626
Fragments arranged by size:
2626 1713 1487 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KasI G'GCGC_C
Cuts at: 2014 2392 2014
Size: 378 5785
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 2445 3238 5277 5461 1305
Size: 819 321 793 2039 184 2007
Fragments arranged by size:
2039 2007 819 793 321 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4341
Fragments arranged by size:
4341 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2813 4121 4480 4639 518
Size: 648 1647 1308 359 159 2042
Fragments arranged by size:
2042 1647 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2398 2605 3579 4256 5404 5649 1606
Size: 621 171 207 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 621 245 207 171
NaeI GCC'GGC
Cuts at: 2365 2413 2666 2365
Size: 48 253 5862
Fragments arranged by size:
5862 253 48
NarI GG'CG_CC
Cuts at: 2015 2393 2015
Size: 378 5785
NcoI C'CATG_G
Cuts at: 513 1065 2004 2814 2957 513
Size: 552 939 810 143 3719
Fragments arranged by size:
3719 939 810 552 143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NgoMIV G'CCGG_C
Cuts at: 2363 2411 2664 2363
Size: 48 253 5862
Fragments arranged by size:
5862 253 48
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 2835 3521 5066 1132
Size: 97 1606 686 1545 2229
Fragments arranged by size:
2229 1606 1545 686 97
PasI CC'CwG_GG
Cuts at: 1348 2737 2843 1348
Size: 1389 106 4668
Fragments arranged by size:
4668 1389 106
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5261
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 6163
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspOMI G'GGCC_C
Cuts at: 2188 2273 3150 2188
Size: 85 877 5201
Fragments arranged by size:
5201 877 85
PstI C_TGCA'G
Cuts at: 830 2076 2403 2643 2715 3515 830
Size: 1246 327 240 72 800 3478
Fragments arranged by size:
3478 1246 800 327 240 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 2227 2605 2227
Size: 378 5785
SacI G_AGCT'C
Cuts at: 2859 2859
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SbfI CC_TGCA'GG
Cuts at: 2076 3515 2076
Size: 1439 4724
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SexAI A'CCwGG_T
Cuts at: 2421 2421
Size: 6163
SfcI C'TryA_G
Cuts at: 826 1045 2072 2399 2639 2711 3511 4646 5327
Size: 219 1027 327 240 72 800 1135 681
Cuts at: 5327 5518 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1027 800 681 327 240 219
191 72
SfoI GGC'GCC
Cuts at: 2016 2394 2016
Size: 378 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 2023 2278 3490 1872
Size: 151 255 1212 4545
Fragments arranged by size:
4545 1212 255 151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2860 4267 5168 5430 820
Size: 197 926 917 1407 901 262 1553
Fragments arranged by size:
1553 1407 926 917 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 2835 3521 1132
Size: 1703 686 3774
Fragments arranged by size:
3774 1703 686
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6163
StyI C'CwwG_G
Cuts at: 513 1065 2004 2814 2957 3602 513
Size: 552 939 810 143 645 3074
Fragments arranged by size:
3074 939 810 645 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 3397 4190 4207 4360 4545 4990
Size: 1255 761 1056 793 17 153 185 445
Cuts at: 4990 325
Size: 1498
Fragments arranged by size:
1498 1255 1056 793 761 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3177 4409 6121
Size: 274 80 33 51 1443 1200 1232 1712
Cuts at: 6121 96
Size: 138
Fragments arranged by size:
1712 1443 1232 1200 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 6123 6155 2033
Size: 32 4058 32 2041
Fragments arranged by size:
4058 2041 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6163
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 3323 2224
Size: 1099 5064
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 2021 2276 3488 1870
Size: 151 255 1212 4545
Fragments arranged by size:
4545 1212 255 151
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SapI SfiI SrfI SwaI