Restriction map for
pFN21AE1150
(Circular) (Six-base) MAPSORT of: pFN21AE1150.seq Check: 1054 from: 1 to: 6163
pFN21AE1150 6163 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3330 3330
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3400 3506 1937
Size: 1463 106 4594
Fragments arranged by size:
4594 1463 106
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2368 4226 5610 5878 1632
Size: 635 101 1858 1384 268 1917
Fragments arranged by size:
1917 1858 1384 635 268 101
AflII C'TTAA_G
Cuts at: 820 1017 2402 820
Size: 197 1385 4581
Fragments arranged by size:
4581 1385 197
AflIII A'CryG_T
Cuts at: 1203 2983 5062 6039 1203
Size: 1780 2079 977 1327
Fragments arranged by size:
2079 1780 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 2932 1166
Size: 1766 4397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 3906 5478 3906
Size: 1572 4591
ApaLI G'TGCA_C
Cuts at: 2477 4220 5376 2477
Size: 1743 1156 3264
Fragments arranged by size:
3264 1743 1156
ApoI r'AATT_y
Cuts at: 1493 2513 2681 2878 2907 3159 3411 3472 3698
Size: 1020 168 197 29 252 252 61 226
Cuts at: 3698 3850 5959 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1020 252 252 226 197 168
152 61 29
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 3052 3488 1870
Size: 73 1109 436 4545
Fragments arranged by size:
4545 1109 436 73
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3484 4939 618
Size: 325 217 627 1697 1455 1842
Fragments arranged by size:
1842 1697 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3482 1391
Size: 2091 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2374 3209 928
Size: 971 475 835 3882
Fragments arranged by size:
3882 971 835 475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2313 4055 5265 2313
Size: 1742 1210 3211
Fragments arranged by size:
3211 1742 1210
BclI T'GATC_A
Cuts at: 1548 2896 1548
Size: 1348 4815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4774 136
Size: 107 122 71 631 605 3102 1525
Fragments arranged by size:
3102 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3580 3580
Size: 6163
Bme1580I G_kGCm'C
Cuts at: 1790 2481 4224 5380 1790
Size: 691 1743 1156 2573
Fragments arranged by size:
2573 1743 1156 691
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 3894 4852
Size: 416 53 83 399 24 2012 465 958
Cuts at: 4852 442
Size: 1753
Fragments arranged by size:
2012 1753 958 465 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2532 4823 1239
Size: 998 295 2291 2579
Fragments arranged by size:
2579 2291 998 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3954 493
Size: 711 2750 2702
Fragments arranged by size:
2750 2702 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3970 4352 4987 275
Size: 53 83 186 936 2437 382 635 1451
Fragments arranged by size:
2437 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3335 4596 4994 5268 5415 5826 3335
Size: 1261 398 274 147 411 3672
Fragments arranged by size:
3672 1261 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2530 2560 1178
Size: 30 165 30 1127 30 4781
Fragments arranged by size:
4781 1127 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 1186
Size: 991 5172
BseYI C'CCAG_C
Cuts at: 5366 5862 5366
Size: 496 5667
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5590
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 4224 4309 5380 2481
Size: 1743 85 1071 3264
Fragments arranged by size:
3264 1743 1071 85
BsmI GAATG_Cn'
Cuts at: 3443 3674 3767 3443
Size: 231 93 5839
Fragments arranged by size:
5839 231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2481 3482 4224 4309 5380 1391
Size: 399 691 1001 742 85 1071 2174
Fragments arranged by size:
2174 1071 1001 742 691 399 85
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3330 3518 844
Size: 2486 188 3489
Fragments arranged by size:
3489 2486 188
BsrBI CCG'CTC
Cuts at: 1394 1823 4050 1394
Size: 429 2227 3507
Fragments arranged by size:
3507 2227 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4807 4994 1700
Size: 110 2997 187 2869
Fragments arranged by size:
2997 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2560 96
Size: 2464 3699
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 4223 5235 4223
Size: 1012 5151
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6163
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstXI CCAn_nnnn'nTGG
Cuts at: 2166 2166
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2867 3493 3860 4245 4262 5714 5800 5812
Size: 895 626 367 385 17 1452 86 12
Cuts at: 5812 6158 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 895 626 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 513
Size: 552 939 22 4650
Fragments arranged by size:
4650 939 552 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 2735 3679 4473 4493 2735
Size: 944 794 20 4405
Fragments arranged by size:
4405 944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3262 3297 529
Size: 35 2698 35 3395
Fragments arranged by size:
3395 2698 35 35
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3031 3610 4499
Size: 54 1425 50 275 18 1201 579 889
Cuts at: 4499 6128 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1201 889 579 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4093 1353
Size: 2740 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2267 2368 2532 4226 4823 5610
Size: 393 605 30 101 164 1694 597 787
Cuts at: 5610 5878 1239
Size: 268 1524
Fragments arranged by size:
1694 1524 787 605 597 393 268 164
101 30
EcoRI G'AATT_C
Cuts at: 3159 3411 3472 3850 3159
Size: 252 61 378 5472
Fragments arranged by size:
5472 378 252 61
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 5310 1678
Size: 3632 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2337 2369 4804 4836 4878
Size: 32 135 32 765 32 2435 32 42
Cuts at: 4878 4910 1373
Size: 32 2626
Fragments arranged by size:
2626 2435 765 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5277 5461 1305
Size: 3972 184 2007
Fragments arranged by size:
3972 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3033 10
Size: 54 1425 343 1201 3140
Fragments arranged by size:
3140 1425 1201 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2664 2932 4121 4480 4639 518
Size: 648 1498 268 1189 359 159 2042
Fragments arranged by size:
2042 1498 1189 648 359 268 159
MspA1I CmG'CkG
Cuts at: 1606 3579 4256 5404 5649 1606
Size: 1973 677 1148 245 2120
Fragments arranged by size:
2120 1973 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4672
Fragments arranged by size:
4672 939 552
NdeI CA'TA_TG
Cuts at: 387 2649 387
Size: 2262 3901
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 2987 3521 5066 1132
Size: 97 1758 534 1545 2229
Fragments arranged by size:
2229 1758 1545 534 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6163
PciI A'CATG_T
Cuts at: 2983 5062 2983
Size: 2079 4084
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6163
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2254 4232 4264 5745 5777 2222
Size: 32 1978 32 1481 32 2608
Fragments arranged by size:
2608 1978 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PstI C_TGCA'G
Cuts at: 830 3515 830
Size: 2685 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 3050 4411 6123 3050
Size: 1361 1712 3090
Fragments arranged by size:
3090 1712 1361
SfcI C'TryA_G
Cuts at: 826 1045 2200 2338 3511 4646 5327 5518 826
Size: 219 1155 138 1173 1135 681 191 1471
Fragments arranged by size:
1471 1173 1155 1135 681 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 3490 1872
Size: 1618 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 4267 5168 5430 820
Size: 197 926 459 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 459 262 197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 2310 4087 5
Size: 47 2258 1777 2081
Fragments arranged by size:
2258 2081 1777 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6163
StyI C'CwwG_G
Cuts at: 513 1065 2004 3602 513
Size: 552 939 1598 3074
Fragments arranged by size:
3074 1598 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4190 4207 4360 4545 4990 325
Size: 1255 2610 17 153 185 445 1498
Fragments arranged by size:
2610 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2560 2936 3048
Size: 274 80 33 51 1443 583 376 112
Cuts at: 3048 4409 6121 96
Size: 1361 1712 138
Fragments arranged by size:
1712 1443 1361 583 376 274 138 112
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2341 2373 6123 6155 2341
Size: 32 3750 32 2349
Fragments arranged by size:
3750 2349 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6163
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 3488 1870
Size: 1618 4545
XmnI GAAnn'nnTTC
Cuts at: 2786 2954 3122 3206 3347 3411 4292 2786
Size: 168 168 84 141 64 881 4657
Fragments arranged by size:
4657 881 168 168 141 84 64
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI