Restriction map for
pFN21AE1147
(Circular) (Six-base) MAPSORT of: pFN21AE1147.seq Check: 5791 from: 1 to: 6091
pFN21AE1147 6091 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 3252 4154 5538 5806 1632
Size: 720 900 902 1384 268 1917
Fragments arranged by size:
1917 1384 902 900 720 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 2699 3834 5406 2699
Size: 1135 1572 3384
Fragments arranged by size:
3384 1572 1135
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2178 2830 3400 3626 3778 5887 1493
Size: 685 652 570 226 152 2109 1697
Fragments arranged by size:
2109 1697 685 652 570 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 3416 1870
Size: 73 181 1292 4545
Fragments arranged by size:
4545 1292 181 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 3410 1391
Size: 826 1193 4072
Fragments arranged by size:
4072 1193 826
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 3155 928
Size: 971 326 930 3864
Fragments arranged by size:
3864 971 930 326
BbvCI CC'TCA_GC
Cuts at: 3289 3289
Size: 6091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2635 4152 5308 1790
Size: 845 1517 1156 2573
Fragments arranged by size:
2573 1517 1156 845
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 3822 4780
Size: 416 53 83 399 24 1550 855 958
Cuts at: 4780 442
Size: 1753
Fragments arranged by size:
1753 1550 958 855 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2703 4751 1239
Size: 1464 2048 2579
Fragments arranged by size:
2579 2048 1464
Bpu10I CC'TnA_GC
Cuts at: 3289 3289
Size: 6091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3368 4216 5081 5379 3368
Size: 848 865 298 4080
Fragments arranged by size:
4080 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2293 2441 4754 882
Size: 617 794 148 2313 2219
Fragments arranged by size:
2313 2219 794 617 148
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 1186
Size: 1273 4818
BseYI C'CCAG_C
Cuts at: 5294 5790 5294
Size: 496 5595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 2065 3602 3695 2065
Size: 1537 93 4461
Fragments arranged by size:
4461 1537 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2635 3410 4152 4237 5308 1391
Size: 399 427 418 775 742 85 1071 2174
Fragments arranged by size:
2174 1071 775 742 427 418 399 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 2974 3446 844
Size: 1467 663 472 3489
Fragments arranged by size:
3489 1467 663 472
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2891 794
Size: 2097 3994
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 3209 2476
Size: 733 5358
BstYI r'GATC_y
Cuts at: 1972 2731 3421 3788 4173 4190 5642 5728 5740
Size: 759 690 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 759 690 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 4135
Fragments arranged by size:
4135 939 552 465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 2102 3607 4401 4421 2102
Size: 1505 794 20 3772
Fragments arranged by size:
3772 1505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2552 2587 529
Size: 35 1988 35 4033
Fragments arranged by size:
4033 1988 35 35
DraI TTT'AAA
Cuts at: 3084 3395 3084
Size: 311 5780
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056
Size: 54 1425 50 275 18 1708 889 1629
Cuts at: 6056 8
Size: 43
Fragments arranged by size:
1708 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4021 1353
Size: 2668 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2703 3252 4154 4751 5538 5806
Size: 393 720 351 549 902 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1524 902 787 720 597 549 393 351
268
EcoO109I rG'GnC_Cy
Cuts at: 2260 3236 2260
Size: 976 5115
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2123 3179 2123
Size: 1056 5035
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4732 4764 4806 4838 1373
Size: 32 135 32 3160 32 42 32 2626
Fragments arranged by size:
3160 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4269
Fragments arranged by size:
4269 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 4049 4408 4567 518
Size: 648 1308 1575 359 159 2042
Fragments arranged by size:
2042 1575 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2723 3507 4184 5332 5577 1606
Size: 1117 784 677 1148 245 2120
Fragments arranged by size:
2120 1148 1117 784 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 4135
Fragments arranged by size:
4135 939 552 465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3449 4994 1132
Size: 97 2220 1545 2229
Fragments arranged by size:
2229 2220 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PfoI T'CCnGG_A
Cuts at: 3187 3187
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 3236 2260
Size: 976 5115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2704 3443 830
Size: 1874 739 3478
Fragments arranged by size:
3478 1874 739
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2723 2723
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 3250 4339 6051 3250
Size: 1089 1712 3290
Fragments arranged by size:
3290 1712 1089
SfcI C'TryA_G
Cuts at: 826 1045 2700 3439 4574 5255 5446 826
Size: 219 1655 739 1135 681 191 1471
Fragments arranged by size:
1655 1471 1135 739 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3383 4195 5096 5358 820
Size: 197 926 1440 812 901 262 1553
Fragments arranged by size:
1553 1440 926 901 812 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 2864 152
Size: 2712 3379
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 3105 3530 513
Size: 552 939 465 636 425 3074
Fragments arranged by size:
3074 939 636 552 465 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 4118 4135 4288 4473 4918 325
Size: 1255 608 1930 17 153 185 445 1498
Fragments arranged by size:
1930 1498 1255 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3248 4337 6049
Size: 274 80 33 51 1443 1271 1089 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
1712 1443 1271 1089 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 3146 1316
Size: 1830 4261
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2833 3037 4220 2833
Size: 204 1183 4704
Fragments arranged by size:
4704 1183 204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI