Restriction map for
pFN21AE1144
(Circular) (Six-base) MAPSORT of: pFN21AE1144.seq Check: 6172 from: 1 to: 5989
pFN21AE1144 5989 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 3073 4116 4489 1563
Size: 1510 1043 373 3063
Fragments arranged by size:
3063 1510 1043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2397 4052 5436 5704 1632
Size: 476 289 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 476 289 268
AflII C'TTAA_G
Cuts at: 820 1017 2534 2680 820
Size: 197 1517 146 4129
Fragments arranged by size:
4129 1517 197 146
AflIII A'CryG_T
Cuts at: 1203 2749 4888 5865 1203
Size: 1546 2139 977 1327
Fragments arranged by size:
2139 1546 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2987 3091 1184
Size: 1803 104 4082
Fragments arranged by size:
4082 1803 104
AlwNI CAG_nnn'CTG
Cuts at: 3732 5304 3732
Size: 1572 4417
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 2243 2900 3082 3298 3524 3676 5785 1493
Size: 750 657 182 216 226 152 2109 1697
Fragments arranged by size:
2109 1697 750 657 226 216 182 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 3314 1870
Size: 73 1371 4545
Fragments arranged by size:
4545 1371 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2538 2571 2538
Size: 33 5956
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3310 4765 618
Size: 325 217 627 1523 1455 1842
Fragments arranged by size:
1842 1523 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2176 3308 1391
Size: 785 1132 4072
Fragments arranged by size:
4072 1132 785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2520 2730 928
Size: 971 621 210 4187
Fragments arranged by size:
4187 971 621 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 1903
Size: 34 2243 34 3678
Fragments arranged by size:
3678 2243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2660 3881 5091 2660
Size: 1221 1210 3558
Fragments arranged by size:
3558 1221 1210
BclI T'GATC_A
Cuts at: 1548 2298 2665 1548
Size: 750 367 4872
Fragments arranged by size:
4872 750 367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4600 136
Size: 107 122 71 631 605 2928 1525
Fragments arranged by size:
2928 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 3406 3406
Size: 5989
Bme1580I G_kGCm'C
Cuts at: 1790 4050 5206 1790
Size: 2260 1156 2573
Fragments arranged by size:
2573 2260 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3241 3720 4678
Size: 416 53 83 399 24 1824 479 958
Cuts at: 4678 442
Size: 1753
Fragments arranged by size:
1824 1753 958 479 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2535 2625 4649 1239
Size: 1296 90 2024 2579
Fragments arranged by size:
2579 2024 1296 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 4114 4979 5277 2588
Size: 1526 865 298 3300
Fragments arranged by size:
3300 1526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5764
Fragments arranged by size:
5764 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2028 1186
Size: 842 5147
BseYI C'CCAG_C
Cuts at: 2832 5192 5688 2832
Size: 2360 496 3133
Fragments arranged by size:
3133 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2475 1163
Size: 573 739 4677
Fragments arranged by size:
4677 739 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4050 4135 5206 4050
Size: 85 1071 4833
Fragments arranged by size:
4833 1071 85
BsmI GAATG_Cn'
Cuts at: 2092 3500 3593 2092
Size: 1408 93 4488
Fragments arranged by size:
4488 1408 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 3308 4050 4135 5206 1391
Size: 399 386 1132 742 85 1071 2174
Fragments arranged by size:
2174 1132 1071 742 399 386 85
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 3878 5608 3878
Size: 1730 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 2938 3344 844
Size: 1792 302 406 3489
Fragments arranged by size:
3489 1792 406 302
BsrBI CCG'CTC
Cuts at: 1394 1823 3876 1394
Size: 429 2053 3507
Fragments arranged by size:
3507 2053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3063 4484 4658 65
Size: 1183 1815 1421 174 1396
Fragments arranged by size:
1815 1421 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4633 4820 1700
Size: 110 2823 187 2869
Fragments arranged by size:
2869 2823 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 3319 3686 4071 4088 5540 5626 5638 5984
Size: 1347 367 385 17 1452 86 12 346
Cuts at: 5984 1972
Size: 1977
Fragments arranged by size:
1977 1452 1347 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 3505 4299 4319 3505
Size: 794 20 5175
Fragments arranged by size:
5175 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 2149 2421 3293 2149
Size: 272 872 4845
Fragments arranged by size:
4845 872 272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2583 2977 3038
Size: 54 1425 50 275 18 753 394 61
Cuts at: 3038 3157 3436 4325 5954 8
Size: 119 279 889 1629 43
Fragments arranged by size:
1629 1425 889 753 394 279 275 119
61 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2014 2166 2819 3045 3919 1353
Size: 661 152 653 226 874 3423
Fragments arranged by size:
3423 874 661 653 226 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2397 2535 2625 4052 4649 5436
Size: 393 476 289 138 90 1427 597 787
Cuts at: 5436 5704 1239
Size: 268 1524
Fragments arranged by size:
1524 1427 787 597 476 393 289 268
138 90
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2497 2990 2497
Size: 493 5496
FspI TGC'GCA
Cuts at: 2771 4495 2771
Size: 1724 4265
HaeII r_GCGC'y
Cuts at: 1678 5136 1678
Size: 3458 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2549 2581 2611
Size: 32 135 32 809 32 136 32 30
Cuts at: 2611 2643 4630 4662 4704 4736 1373
Size: 32 1987 32 42 32 2626
Fragments arranged by size:
2626 1987 809 136 135 42 32 32
32 32 32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3579 669
Size: 988 281 1395 246 3079
Fragments arranged by size:
3079 1395 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2455 748
Size: 1707 4282
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5103 5287 1305
Size: 3798 184 2007
Fragments arranged by size:
3798 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2585 10
Size: 54 1425 343 753 3414
Fragments arranged by size:
3414 1425 753 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3947 4306 4465 518
Size: 648 2781 359 159 2042
Fragments arranged by size:
2781 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2375 2625 3405 4082 5230 5475 1606
Size: 769 250 780 677 1148 245 2120
Fragments arranged by size:
2120 1148 780 769 677 250 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5437
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2221 2753 2997 3347 4892 1132
Size: 97 992 532 244 350 1545 2229
Fragments arranged by size:
2229 1545 992 532 350 244 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 2749 4888 2749
Size: 2139 3850
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5989
PfoI T'CCnGG_A
Cuts at: 2794 2794
Size: 5989
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PstI C_TGCA'G
Cuts at: 830 2059 3341 830
Size: 1229 1282 3478
Fragments arranged by size:
3478 1282 1229
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2375 2625 2375
Size: 250 5739
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 2577 3192 4237 5949 2577
Size: 615 1045 1712 2617
Fragments arranged by size:
2617 1712 1045 615
SexAI A'CCwGG_T
Cuts at: 2205 2205
Size: 5989
SfcI C'TryA_G
Cuts at: 826 1045 2055 3337 4472 5153 5344 826
Size: 219 1010 1282 1135 681 191 1471
Fragments arranged by size:
1471 1282 1135 1010 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2534 2603 2680 4093 4994 5256
Size: 197 926 591 69 77 1413 901 262
Cuts at: 5256 820
Size: 1553
Fragments arranged by size:
1553 1413 926 901 591 262 197 77
69
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 2578 3013 152
Size: 2426 435 3128
Fragments arranged by size:
3128 2426 435
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 2153 3913 5
Size: 47 2101 1760 2081
Fragments arranged by size:
2101 2081 1760 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 3428 513
Size: 552 2363 3074
Fragments arranged by size:
3074 2363 552
SwaI ATTT'AAAT
Cuts at: 2149 2149
Size: 5989
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2872 2951 4016 4033 4186 4371 4816
Size: 1255 1292 79 1065 17 153 185 445
Cuts at: 4816 325
Size: 1498
Fragments arranged by size:
1498 1292 1255 1065 445 185 153 79
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2575 2943 3190
Size: 274 80 33 51 1443 598 368 247
Cuts at: 3190 4235 5947 96
Size: 1045 1712 138
Fragments arranged by size:
1712 1443 1045 598 368 274 247 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5949 5981 5949
Size: 32 5957
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2945 2945
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI