Restriction map for
pFN21AE1142
(Circular) (Six-base) MAPSORT of: pFN21AE1142.seq Check: 3858 from: 1 to: 5965
pFN21AE1142 5965 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 1937
Size: 1371 4594
Acc65I G'GTAC_C
Cuts at: 2883 3286 2883
Size: 403 5562
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 2756 2795 4028 5412 5680 1632
Size: 995 129 39 1233 1384 268 1917
Fragments arranged by size:
1917 1384 1233 995 268 129 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 2625 3258 4864 5841 1203
Size: 1422 633 1606 977 1327
Fragments arranged by size:
1606 1422 1327 977 633
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 2295 1166
Size: 1129 4836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5965
AlwNI CAG_nnn'CTG
Cuts at: 2236 2494 3708 5280 2236
Size: 258 1214 1572 2921
Fragments arranged by size:
2921 1572 1214 258
ApaI G_GGCC'C
Cuts at: 2196 2196
Size: 5965
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 3223 3274 3500 3652 5761 1493
Size: 1730 51 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2195 3290 1870
Size: 73 159 93 1095 4545
Fragments arranged by size:
4545 1095 159 93 73
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2689 2883 3286 4741
Size: 325 217 627 333 569 194 403 1455
Cuts at: 4741 618
Size: 1842
Fragments arranged by size:
1842 1455 627 569 403 333 325 217
194
BanII G_rGCy'C
Cuts at: 1391 2057 2196 2568 3054 3284 1391
Size: 666 139 372 486 230 4072
Fragments arranged by size:
4072 666 486 372 230 139
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 5965
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3128 928
Size: 971 1229 3765
Fragments arranged by size:
3765 1229 971
BbvCI CC'TCA_GC
Cuts at: 2780 2780
Size: 5965
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4576 136
Size: 107 122 71 631 605 2904 1525
Fragments arranged by size:
2904 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5960 5960
Size: 5965
BlpI GC'TnA_GC
Cuts at: 2481 3382 2481
Size: 901 5064
Bme1580I G_kGCm'C
Cuts at: 1790 2102 2196 4026 5182 1790
Size: 312 94 1830 1156 2573
Fragments arranged by size:
2573 1830 1156 312 94
BmgBI CAC'GTC
Cuts at: 1318 2593 1318
Size: 1275 4690
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2281 3696 4654
Size: 416 53 83 399 24 864 1415 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1415 958 864 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2447 2128
Size: 319 5646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3131 3235 4625 1239
Size: 1892 104 1390 2579
Fragments arranged by size:
2579 1892 1390 104
Bpu10I CC'TnA_GC
Cuts at: 2780 2780
Size: 5965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3115 4090 4955 5253 3115
Size: 975 865 298 3827
Fragments arranged by size:
3827 975 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4628 882
Size: 617 3129 2219
Fragments arranged by size:
3129 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2335 2620 2632 3112 3756 493
Size: 711 1131 285 12 480 644 2702
Fragments arranged by size:
2702 1131 711 644 480 285 12
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2244 3772 4154
Size: 53 83 186 936 588 123 1528 382
Cuts at: 4154 4789 275
Size: 635 1451
Fragments arranged by size:
1528 1451 936 635 588 382 186 123
83 53
BsaWI w'CCGG_w
Cuts at: 4398 4796 5070 5217 5628 4398
Size: 398 274 147 411 4735
Fragments arranged by size:
4735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5740
Fragments arranged by size:
5740 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2771 1186
Size: 1054 531 4380
Fragments arranged by size:
4380 1054 531
BseYI C'CCAG_C
Cuts at: 2014 2417 2438 2494 5168 5664 2014
Size: 403 21 56 2674 496 2315
Fragments arranged by size:
2674 2315 496 403 56 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2056 1163
Size: 573 320 5072
Fragments arranged by size:
5072 573 320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2118 3415 4176 5204 1540
Size: 275 186 117 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186 117
BsiHKAI G_wGCw'C
Cuts at: 3054 3078 4026 4111 5182 3054
Size: 24 948 85 1071 3837
Fragments arranged by size:
3837 1071 948 85 24
BsmI GAATG_Cn'
Cuts at: 3476 3569 3476
Size: 93 5872
BsmBI CGTCTCn'nnnn_
Cuts at: 2543 2543
Size: 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2102 2196 2568 3054 3078 3284
Size: 399 267 45 94 372 486 24 206
Cuts at: 3284 4026 4111 5182 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 399 372 267 206
94 85 45 24
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 844
Size: 2476 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 1394
Size: 429 2029 3507
Fragments arranged by size:
3507 2029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4460 4634 65
Size: 1183 3212 174 1396
Fragments arranged by size:
3212 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2117 2886 2978 4609 4796 1700
Size: 110 307 769 92 1631 187 2869
Fragments arranged by size:
2869 1631 769 307 187 110 92
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 3718
BssHII G'CGCG_C
Cuts at: 1925 2611 1925
Size: 686 5279
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2079 2236 794
Size: 1285 157 4523
Fragments arranged by size:
4523 1285 157
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 2865 3246 3295 3662 4047 4064 5516 5602
Size: 893 381 49 367 385 17 1452 86
Cuts at: 5602 5614 5960 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 893 385 381 367 346 86
49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2149 2350 513
Size: 552 939 145 201 4128
Fragments arranged by size:
4128 939 552 201 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2653 487
Size: 683 56 1427 3799
Fragments arranged by size:
3799 1427 683 56
BtsI GCAGTG_nn'
Cuts at: 3481 4275 4295 3481
Size: 794 20 5151
Fragments arranged by size:
5151 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3090 3269 3090
Size: 179 5786
DraIII CAC_nnn'GTG
Cuts at: 2079 2079
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4972 809
Size: 507 3656 1802
Fragments arranged by size:
3656 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2115 2131
Size: 54 1425 50 275 18 213 72 16
Cuts at: 2131 3412 4301 5930 8
Size: 1281 889 1629 43
Fragments arranged by size:
1629 1425 1281 889 275 213 72 54
50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2115 3412 1537
Size: 275 303 1297 4090
Fragments arranged by size:
4090 1297 303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2907 2948 3895 1353
Size: 1554 41 947 3423
Fragments arranged by size:
3423 1554 947 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2674 4552 4926 5072 387
Size: 1064 138 435 650 1878 374 146 1280
Fragments arranged by size:
1878 1280 1064 650 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3052 3052
Size: 5965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2627 2756 2795 3131 3235 4028 4625
Size: 393 995 129 39 336 104 793 597
Cuts at: 4625 5412 5680 1239
Size: 787 268 1524
Fragments arranged by size:
1524 995 793 787 597 393 336 268
129 104 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2363 2705 2045
Size: 318 342 5305
Fragments arranged by size:
5305 342 318
EcoO109I rG'GnC_Cy
Cuts at: 2192 2426 2192
Size: 234 5731
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 2124 2179 2735 5112 1678
Size: 446 55 556 2377 2531
Fragments arranged by size:
2531 2377 556 446 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3041 3073 4606 4638 4680
Size: 32 135 32 1469 32 1533 32 42
Cuts at: 4680 4712 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 1469 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 3309 3555 669
Size: 988 281 637 734 246 3079
Fragments arranged by size:
3079 988 734 637 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5965
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 5965
KpnI G_GTAC'C
Cuts at: 2887 3290 2887
Size: 403 5562
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 3258 5841 3258
Size: 2583 3382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5079 5263 1305
Size: 3774 184 2007
Fragments arranged by size:
3774 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4143
Fragments arranged by size:
4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2295 2474 2630 2906 3923 4282 4441
Size: 648 1129 179 156 276 1017 359 159
Cuts at: 4441 518
Size: 2042
Fragments arranged by size:
2042 1129 1017 648 359 276 179 159
156
MspA1I CmG'CkG
Cuts at: 1606 2059 2070 2161 2233 2421 2455 2464 2494
Size: 453 11 91 72 188 34 9 30
Cuts at: 2494 2509 2562 2656 2788 2881 3381 4058 5206
Size: 15 53 94 132 93 500 677 1148
Cuts at: 5206 5451 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 500 453 245 188 132
94 93 91 72 53 34 30 15
11 9
NaeI GCC'GGC
Cuts at: 2119 2980 2119
Size: 861 5104
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 5965
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4474
Fragments arranged by size:
4474 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NgoMIV G'CCGG_C
Cuts at: 2117 2978 2117
Size: 861 5104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NruI TCG'CGA
Cuts at: 2223 3066 2223
Size: 843 5122
NspI r_CATG'y
Cuts at: 1132 1229 2344 2506 2629 2839 3323 4868 1132
Size: 97 1115 162 123 210 484 1545 2229
Fragments arranged by size:
2229 1545 1115 484 210 162 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 2625 4864 2625
Size: 2239 3726
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2164 1108
Size: 1056 4909
PfoI T'CCnGG_A
Cuts at: 2303 2303
Size: 5965
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PmlI CAC'GTG
Cuts at: 2620 2620
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3131 3535 3131
Size: 404 5561
PspOMI G'GGCC_C
Cuts at: 2192 2192
Size: 5965
PstI C_TGCA'G
Cuts at: 830 2880 3083 3317 830
Size: 2050 203 234 3478
Fragments arranged by size:
3478 2050 234 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 2161 2233 2494 2161
Size: 72 261 5632
Fragments arranged by size:
5632 261 72
SacI G_AGCT'C
Cuts at: 3054 3054
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 2027 4213 5925 2027
Size: 2186 1712 2067
Fragments arranged by size:
2186 2067 1712
SfcI C'TryA_G
Cuts at: 826 1045 2876 3079 3313 4448 5129 5320 826
Size: 219 1831 203 234 1135 681 191 1471
Fragments arranged by size:
1831 1471 1135 681 234 219 203 191
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 5965
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3094 4069 4970 5232 820
Size: 197 926 1151 975 901 262 1553
Fragments arranged by size:
1553 1151 975 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 2369 1517
Size: 852 5113
StyI C'CwwG_G
Cuts at: 513 1065 2004 2751 3404 513
Size: 552 939 747 653 3074
Fragments arranged by size:
3074 939 747 653 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2452 3992 4009 4162 4347 4792 325
Size: 1255 872 1540 17 153 185 445 1498
Fragments arranged by size:
1540 1498 1255 872 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 2034 2343
Size: 274 80 33 51 1443 48 9 309
Cuts at: 2343 4211 5923 96
Size: 1868 1712 138
Fragments arranged by size:
1868 1712 1443 309 274 138 80 51
48 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 3200 1316
Size: 1884 4081
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NsiI
PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI