Restriction map for
pFN21AE1139
(Circular) (Six-base) MAPSORT of: pFN21AE1139.seq Check: 868 from: 1 to: 5896
pFN21AE1139 5896 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 3239 1937
Size: 948 354 4594
Fragments arranged by size:
4594 948 354
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2294 3959 5343 5611 1632
Size: 503 159 1665 1384 268 1917
Fragments arranged by size:
1917 1665 1384 503 268 159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 3193 4795 5772 1203
Size: 1990 1602 977 1327
Fragments arranged by size:
1990 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5896
AlwNI CAG_nnn'CTG
Cuts at: 2092 2113 2449 3639 5211 2092
Size: 21 336 1190 1572 2777
Fragments arranged by size:
2777 1572 1190 336 21
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 2219 3205 3431 3583 5692 1493
Size: 726 986 226 152 2109 1697
Fragments arranged by size:
2109 1697 986 726 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 3221 1870
Size: 73 1143 135 4545
Fragments arranged by size:
4545 1143 135 73
AvrII C'CTAG_G
Cuts at: 2350 2350
Size: 5896
BamHI G'GATC_C
Cuts at: 2159 2244 3226 3593 2159
Size: 85 982 367 4462
Fragments arranged by size:
4462 982 367 85
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3217 4672 618
Size: 325 217 627 1430 1455 1842
Fragments arranged by size:
1842 1455 1430 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2325 2697 2904 3215 1391
Size: 934 372 207 311 4072
Fragments arranged by size:
4072 934 372 311 207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 2754 928
Size: 971 396 459 4070
Fragments arranged by size:
4070 971 459 396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 1903
Size: 34 2150 34 3678
Fragments arranged by size:
3678 2150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2548 2945 3788 4998 2548
Size: 397 843 1210 3446
Fragments arranged by size:
3446 1210 843 397
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BfrBI ATG'CAT
Cuts at: 2390 2390
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4507 136
Size: 107 122 71 631 605 2835 1525
Fragments arranged by size:
2835 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 3957 5113 1790
Size: 2167 1156 2573
Fragments arranged by size:
2573 2167 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5896
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 2293 3627
Size: 416 53 83 399 24 608 268 1334
Cuts at: 3627 4585 442
Size: 958 1753
Fragments arranged by size:
1753 1334 958 608 416 399 268 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 4556 1239
Size: 845 2472 2579
Fragments arranged by size:
2579 2472 845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4021 4886 5184 4021
Size: 865 298 4733
Fragments arranged by size:
4733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2673 3181 4559 882
Size: 617 1174 508 1378 2219
Fragments arranged by size:
2219 1378 1174 617 508
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 2421 5890 2421
Size: 3469 2427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3083 3703 4085 4720
Size: 53 83 186 936 1550 620 382 635
Cuts at: 4720 275
Size: 1451
Fragments arranged by size:
1550 1451 936 635 620 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2490 2700 3066 4329 4727 5001 5148 5559 2490
Size: 210 366 1263 398 274 147 411 2827
Fragments arranged by size:
2827 1263 411 398 366 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 1178
Size: 30 165 30 1152 30 4489
Fragments arranged by size:
4489 1152 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2063 2687 2705 3104 3113 3169 1186
Size: 838 39 624 18 399 9 56 3913
Fragments arranged by size:
3913 838 624 399 56 39 18 9
BseYI C'CCAG_C
Cuts at: 2511 5099 5595 2511
Size: 2588 496 2812
Fragments arranged by size:
2812 2588 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2588 1163
Size: 573 852 4471
Fragments arranged by size:
4471 852 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2491 3346 4107 5135 1540
Size: 275 186 490 855 761 1028 2301
Fragments arranged by size:
2301 1028 855 761 490 275 186
BsiHKAI G_wGCw'C
Cuts at: 2697 3957 4042 5113 2697
Size: 1260 85 1071 3480
Fragments arranged by size:
3480 1260 1071 85
BsmI GAATG_Cn'
Cuts at: 3407 3500 3407
Size: 93 5803
BsmBI CGTCTCn'nnnn_
Cuts at: 2833 2833
Size: 5896
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 2697 2904 3215 3957 4042 5113
Size: 399 535 372 207 311 742 85 1071
Cuts at: 5113 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 535 399 372 311 207
85
BspEI T'CCGG_A
Cuts at: 2700 5559 2700
Size: 2859 3037
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2691 3251 844
Size: 1847 560 3489
Fragments arranged by size:
3489 1847 560
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 2646 4391 4565 65
Size: 1183 1044 354 1745 174 1396
Fragments arranged by size:
1745 1396 1183 1044 354 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4540 4727 1700
Size: 110 2730 187 2869
Fragments arranged by size:
2869 2730 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5896
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 2159 2244 3069 3226 3593 3978 3995 5447
Size: 187 85 825 157 367 385 17 1452
Cuts at: 5447 5533 5545 5891 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 825 385 367 346 187 157
86 85 17 12
BstZ17I GTA'TAC
Cuts at: 2886 2886
Size: 5896
BtgI C'CryG_G
Cuts at: 513 1065 2004 2205 2550 2889 2905 513
Size: 552 939 201 345 339 16 3504
Fragments arranged by size:
3504 939 552 345 339 201 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 3412 4206 4226 3412
Size: 794 20 5082
Fragments arranged by size:
5082 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 2737 3200 2737
Size: 463 5433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3343 4232 5861
Size: 54 1425 50 275 18 1513 889 1629
Cuts at: 5861 8
Size: 43
Fragments arranged by size:
1629 1513 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 3826 1353
Size: 1478 995 3423
Fragments arranged by size:
3423 1478 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2695 2695
Size: 5896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2135 2294 3959 4556 5343 5611
Size: 393 452 51 159 1665 597 787 268
Cuts at: 5611 1239
Size: 1524
Fragments arranged by size:
1665 1524 787 597 452 393 268 159
51
EcoNI CCTnn'n_nnAGG
Cuts at: 2687 2687
Size: 5896
EcoO109I rG'GnC_Cy
Cuts at: 3136 3136
Size: 5896
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 5043 1678
Size: 3365 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3061 3093 4537 4569 4611
Size: 32 135 32 1489 32 1444 32 42
Cuts at: 4611 4643 1373
Size: 32 2626
Fragments arranged by size:
2626 1489 1444 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2098 3104 5010 5194 1305
Size: 793 1006 1906 184 2007
Fragments arranged by size:
2007 1906 1006 793 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4074
Fragments arranged by size:
4074 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 2651 3854 4213 4372 518
Size: 648 1419 66 1203 359 159 2042
Fragments arranged by size:
2042 1419 1203 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 3312 3989 5137 5382 1606
Size: 1706 677 1148 245 2120
Fragments arranged by size:
2120 1706 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2205 513
Size: 552 939 201 4204
Fragments arranged by size:
4204 939 552 201
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NsiI A_TGCA'T
Cuts at: 2392 2392
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2185 3254 4799 1132
Size: 97 956 1069 1545 2229
Fragments arranged by size:
2229 1545 1069 956 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5896
PfoI T'CCnGG_A
Cuts at: 2937 2937
Size: 5896
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 3136
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PstI C_TGCA'G
Cuts at: 830 3248 830
Size: 2418 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
SacI G_AGCT'C
Cuts at: 2697 2697
Size: 5896
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SanDI GG'GwC_CC
Cuts at: 3136 3136
Size: 5896
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SfcI C'TryA_G
Cuts at: 826 1045 3244 4379 5060 5251 826
Size: 219 2199 1135 681 191 1471
Fragments arranged by size:
2199 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4000 4901 5163 820
Size: 197 926 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 3254 1132
Size: 2122 3774
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 3014 1517
Size: 1497 4399
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 2350 3335 513
Size: 552 939 201 145 985 3074
Fragments arranged by size:
3074 985 939 552 201 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2805 3038 3923 3940 4093 4278 4723
Size: 1255 1225 233 885 17 153 185 445
Cuts at: 4723 325
Size: 1498
Fragments arranged by size:
1498 1255 1225 885 445 233 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4142 5854 96
Size: 274 80 33 51 1443 2165 1712 138
Fragments arranged by size:
2165 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5856 5888 5856
Size: 32 5864
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5896
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI