Restriction map for
pFN21AE1136
(Circular) (Six-base) MAPSORT of: pFN21AE1136.seq Check: 1073 from: 1 to: 5776
pFN21AE1136 5776 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2709 2709
Size: 5776
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2410 3839 5223 5491 1632
Size: 465 313 1429 1384 268 1917
Fragments arranged by size:
1917 1429 1384 465 313 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2021 3519 5091 2021
Size: 1498 1572 2706
Fragments arranged by size:
2706 1572 1498
ApaI G_GGCC'C
Cuts at: 2289 2638 3025 2289
Size: 349 387 5040
Fragments arranged by size:
5040 387 349
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3005 3085 3311 3463 5572 1493
Size: 1512 80 226 152 2109 1697
Fragments arranged by size:
2109 1697 1512 226 152 80
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2792 3101 1870
Size: 73 849 309 4545
Fragments arranged by size:
4545 849 309 73
BamHI G'GATC_C
Cuts at: 2310 3106 3473 2310
Size: 796 367 4613
Fragments arranged by size:
4613 796 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2289 2638 3025 3041 3095 1391
Size: 898 349 387 16 54 4072
Fragments arranged by size:
4072 898 387 349 54 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 928
Size: 971 636 4169
Fragments arranged by size:
4169 971 636
BbvCI CC'TCA_GC
Cuts at: 2738 2738
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 4387 136
Size: 107 122 71 631 605 365 2350 1525
Fragments arranged by size:
2350 1525 631 605 365 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2289 2638 3025 3837 4993 1790
Size: 499 349 387 812 1156 2573
Fragments arranged by size:
2573 1156 812 499 387 349
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4436 1239
Size: 3197 2579
Bpu10I CC'TnA_GC
Cuts at: 2738 2738
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2892 2907 3901 4766 5064 2892
Size: 15 994 865 298 3604
Fragments arranged by size:
3604 994 865 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5551
Fragments arranged by size:
5551 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 2648 1186
Size: 1319 143 4314
Fragments arranged by size:
4314 1319 143
BseYI C'CCAG_C
Cuts at: 2763 4979 5475 2763
Size: 2216 496 3064
Fragments arranged by size:
3064 2216 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 1163
Size: 573 456 4747
Fragments arranged by size:
4747 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2282 3837 3922 4993 2282
Size: 1555 85 1071 3065
Fragments arranged by size:
3065 1555 1071 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2282 2289 2638 3025 3041 3095 3837
Size: 399 492 7 349 387 16 54 742
Cuts at: 3837 3922 4993 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 387 349 85
54 16 7
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2709 3131 844
Size: 1865 422 3489
Fragments arranged by size:
3489 1865 422
BsrBI CCG'CTC
Cuts at: 1394 1823 2513 3663 1394
Size: 429 690 1150 3507
Fragments arranged by size:
3507 1150 690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2041 2530 2664 4271 4445 65
Size: 1183 793 489 134 1607 174 1396
Fragments arranged by size:
1607 1396 1183 793 489 174 134
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2867 3836 4848 2867
Size: 969 1012 3795
Fragments arranged by size:
3795 1012 969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2677 2677
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2310 3106 3473 3858 3875 5327 5413 5425
Size: 338 796 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 796 385 367 346 338 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 2826 3017 513
Size: 552 939 414 408 191 3272
Fragments arranged by size:
3272 939 552 414 408 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2415 3032
Size: 54 1425 50 275 18 276 309 617
Cuts at: 3032 3223 4112 5741 8
Size: 191 889 1629 43
Fragments arranged by size:
1629 1425 889 617 309 276 275 191
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 2564 2618 2870 2928 3706 1353
Size: 866 345 54 252 58 778 3423
Fragments arranged by size:
3423 866 778 345 252 58 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2734 4363 4737 4883 387
Size: 1064 138 1145 1629 374 146 1280
Fragments arranged by size:
1629 1280 1145 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2410 3839 4436 5223 5491 1239
Size: 393 465 313 1429 597 787 268 1524
Fragments arranged by size:
1524 1429 787 597 465 393 313 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 2435
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2285 2634 2285
Size: 349 5427
EcoRI G'AATT_C
Cuts at: 3005 3085 3463 3005
Size: 80 378 5318
Fragments arranged by size:
5318 378 80
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2573 4923 1678
Size: 895 2350 2531
Fragments arranged by size:
2531 2350 895
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2395 2427 4417 4449 4491
Size: 32 135 32 823 32 1990 32 42
Cuts at: 4491 4523 1373
Size: 32 2626
Fragments arranged by size:
2626 1990 823 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2079 748
Size: 1331 4445
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 4890 5074 1305
Size: 900 2685 184 2007
Fragments arranged by size:
2685 2007 900 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 10
Size: 54 1425 343 276 3678
Fragments arranged by size:
3678 1425 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2675 3734 4093 4252 518
Size: 648 1509 1059 359 159 2042
Fragments arranged by size:
2042 1509 1059 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2980 3192 3869 5017 5262 1606
Size: 1374 212 677 1148 245 2120
Fragments arranged by size:
2120 1374 1148 677 245 212
NcoI C'CATG_G
Cuts at: 513 1065 2004 2826 513
Size: 552 939 822 3463
Fragments arranged by size:
3463 939 822 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2763 1108
Size: 1655 4121
PfoI T'CCnGG_A
Cuts at: 2436 2436
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2486 1613
Size: 873 4903
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2285 2634 3021 2285
Size: 349 387 5040
Fragments arranged by size:
5040 387 349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 5744
PstI C_TGCA'G
Cuts at: 830 2985 3128 830
Size: 2155 143 3478
Fragments arranged by size:
3478 2155 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2980 2980
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2870 2870
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2054 4024 5736 2054
Size: 1970 1712 2094
Fragments arranged by size:
2094 1970 1712
SfcI C'TryA_G
Cuts at: 826 1045 2056 2981 3124 4259 4940 5131 826
Size: 219 1011 925 143 1135 681 191 1471
Fragments arranged by size:
1471 1135 1011 925 681 219 191 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2907 2922 3880 4781 5043 820
Size: 197 926 964 15 958 901 262 1553
Fragments arranged by size:
1553 964 958 926 901 262 197 15
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2748 2826 3215 513
Size: 552 939 744 78 389 3074
Fragments arranged by size:
3074 939 744 552 389 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 2665 2755 3803 3820 3973 4158
Size: 1255 633 452 90 1048 17 153 185
Cuts at: 4158 4603 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1048 633 452 445 185 153
90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 4022 5734
Size: 274 80 33 51 1443 75 1970 1712
Cuts at: 5734 96
Size: 138
Fragments arranged by size:
1970 1712 1443 274 138 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 5736 5768 2283
Size: 32 3421 32 2291
Fragments arranged by size:
3421 2291 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2534 1316
Size: 1218 4558
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI