Restriction map for
pFN21AE1133
(Circular) (Six-base) MAPSORT of: pFN21AE1133.seq Check: 4674 from: 1 to: 5695
pFN21AE1133 5695 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 2483 3016 2483
Size: 533 5162
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3758 5142 5410 1632
Size: 2126 1384 268 1917
Fragments arranged by size:
2126 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 3438 5010 3438
Size: 1572 4123
ApaI G_GGCC'C
Cuts at: 2270 2298 2523 2270
Size: 28 225 5442
Fragments arranged by size:
5442 225 28
ApaLI G'TGCA_C
Cuts at: 2777 3752 4908 2777
Size: 975 1156 3564
Fragments arranged by size:
3564 1156 975
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2489 3020 1870
Size: 73 546 531 4545
Fragments arranged by size:
4545 546 531 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 2483 3016 4471 618
Size: 325 217 627 411 285 533 1455 1842
Fragments arranged by size:
1842 1455 627 533 411 325 285 217
BanII G_rGCy'C
Cuts at: 1391 2076 2270 2290 2298 2523 2916 3014 1391
Size: 685 194 20 8 225 393 98 4072
Fragments arranged by size:
4072 685 393 225 194 98 20 8
BbeI G_GCGC'C
Cuts at: 2202 2202
Size: 5695
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BbvCI CC'TCA_GC
Cuts at: 2052 2052
Size: 5695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2256 2265 2274
Size: 107 122 71 631 605 584 9 9
Cuts at: 2274 2463 2553 4306 136
Size: 189 90 1753 1525
Fragments arranged by size:
1753 1525 631 605 584 189 122 107
90 71 9 9
BglII A'GATC_T
Cuts at: 2889 5690 2889
Size: 2801 2894
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2270 2298 2523 2781 3756 4912 1790
Size: 380 100 28 225 258 975 1156 2573
Fragments arranged by size:
2573 1156 975 380 258 225 100 28
BmgBI CAC'GTC
Cuts at: 1318 2112 1318
Size: 794 4901
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4355 1239
Size: 3116 2579
Bpu10I CC'TnA_GC
Cuts at: 2052 2052
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3820 4685 4983 2846
Size: 974 865 298 3558
Fragments arranged by size:
3558 974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2101 2199 3502 3884
Size: 53 83 186 936 568 98 1303 382
Cuts at: 3884 4519 275
Size: 635 1451
Fragments arranged by size:
1451 1303 936 635 568 382 186 98
83 53
BsaWI w'CCGG_w
Cuts at: 2096 2220 2799 4128 4526 4800 4947 5358 2096
Size: 124 579 1329 398 274 147 411 2433
Fragments arranged by size:
2433 1329 579 411 398 274 147 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 4898 5394 4898
Size: 496 5199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2362 3145 3906 4934 1540
Size: 275 186 361 783 761 1028 2301
Fragments arranged by size:
2301 1028 783 761 361 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2781 3756 3841 4912 2157
Size: 624 975 85 1071 2940
Fragments arranged by size:
2940 1071 975 624 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2157 2170 2270 2290 2298 2523
Size: 399 286 81 13 100 20 8 225
Cuts at: 2523 2781 2916 3014 3756 3841 4912 1391
Size: 258 135 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 286 258 225 135
100 98 85 81 20 13 8
BspEI T'CCGG_A
Cuts at: 2096 2220 2799 5358 2096
Size: 124 579 2559 2433
Fragments arranged by size:
2559 2433 579 124
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3050 844
Size: 2206 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2629 3582 1394
Size: 429 806 953 3507
Fragments arranged by size:
3507 953 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2819 4339 4526 1700
Size: 110 1009 1520 187 2869
Fragments arranged by size:
2869 1520 1009 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 2131 2402 1184
Size: 947 271 4477
Fragments arranged by size:
4477 947 271
BstXI CCAn_nnnn'nTGG
Cuts at: 2773 2854 2773
Size: 81 5614
BstYI r'GATC_y
Cuts at: 1972 2718 2889 3025 3392 3777 3794 5246 5332
Size: 746 171 136 367 385 17 1452 86
Cuts at: 5332 5344 5690 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 746 385 367 346 171 136
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2299 2839 2299
Size: 540 5155
BtgI C'CryG_G
Cuts at: 513 1065 2004 2461 2621 513
Size: 552 939 457 160 3587
Fragments arranged by size:
3587 939 552 457 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2189 2578 2610 3211 4005 4025 2189
Size: 389 32 601 794 20 3859
Fragments arranged by size:
3859 794 601 389 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2833 2833
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2338 2392 2575
Size: 54 1425 50 275 18 508 54 183
Cuts at: 2575 2671 2748 2763 2785 3142 4031 5660 8
Size: 96 77 15 22 357 889 1629 43
Fragments arranged by size:
1629 1425 889 508 357 275 183 96
77 54 54 50 43 22 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2921 3625 1353
Size: 1568 704 3423
Fragments arranged by size:
3423 1568 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4282 4656 4802 387
Size: 1064 138 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3758 4355 5142 5410 1239
Size: 393 2126 597 787 268 1524
Fragments arranged by size:
2126 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2266 2294 2295 2519 2555 2266
Size: 28 1 224 36 5406
Fragments arranged by size:
5406 224 36 28 1
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2202 4842 1678
Size: 524 2640 2531
Fragments arranged by size:
2640 2531 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2854 4336 4368 4410
Size: 32 135 32 1250 32 1482 32 42
Cuts at: 4410 4442 1373
Size: 32 2626
Fragments arranged by size:
2626 1482 1250 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 3039 3285 669
Size: 988 281 541 560 246 3079
Fragments arranged by size:
3079 988 560 541 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KasI G'GCGC_C
Cuts at: 2198 2198
Size: 5695
KpnI G_GTAC'C
Cuts at: 2487 3020 2487
Size: 533 5162
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2577 2673 2750 2765 10
Size: 54 1425 343 745 96 77 15 2940
Fragments arranged by size:
2940 1425 745 343 96 77 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2679 3653 4012 4171 518
Size: 648 1513 974 359 159 2042
Fragments arranged by size:
2042 1513 974 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2373 2565 2691 3111 3788 4936 5181
Size: 445 322 192 126 420 677 1148 245
Cuts at: 5181 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 445 420 322 245 192
126
NarI GG'CG_CC
Cuts at: 2199 2199
Size: 5695
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4204
Fragments arranged by size:
4204 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 3053 4598 1132
Size: 97 1824 1545 2229
Fragments arranged by size:
2229 1824 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2148 2263 2291 1348
Size: 800 115 28 4752
Fragments arranged by size:
4752 800 115 28
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2195 2833 1108
Size: 1087 638 3970
Fragments arranged by size:
3970 1087 638
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2266 2294 2519 2266
Size: 28 225 5442
Fragments arranged by size:
5442 225 28
PstI C_TGCA'G
Cuts at: 830 2249 2564 2889 3047 830
Size: 1419 315 325 158 3478
Fragments arranged by size:
3478 1419 325 315 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2565 2565
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SapI GCTCTTCn'nnn_
Cuts at: 2921 2921
Size: 5695
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2245 2560 2885 3043 4178 4859 5050
Size: 219 1200 315 325 158 1135 681 191
Cuts at: 5050 826
Size: 1471
Fragments arranged by size:
1471 1200 1135 681 325 315 219 191
158
SfiI GGCCn_nnn'nGGCC
Cuts at: 2265 2274 2463 2553 2265
Size: 9 189 90 5407
Fragments arranged by size:
5407 189 90 9
SfoI GGC'GCC
Cuts at: 2200 2200
Size: 5695
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 2491 3022 1872
Size: 619 531 4545
Fragments arranged by size:
4545 619 531
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2861 3799 4700 4962 820
Size: 197 926 918 938 901 262 1553
Fragments arranged by size:
1553 938 926 918 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2547 1517
Size: 1030 4665
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 2523 3134 513
Size: 552 939 465 54 611 3074
Fragments arranged by size:
3074 939 611 552 465 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 3722 3739 3892 4077 4522 325
Size: 1255 560 1582 17 153 185 445 1498
Fragments arranged by size:
1582 1498 1255 560 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3941 5653 96
Size: 274 80 33 51 1443 1964 1712 138
Fragments arranged by size:
1964 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 2758
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 2489 3020 1870
Size: 619 531 4545
Fragments arranged by size:
4545 619 531
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SrfI SwaI