Restriction map for
pFN21AE1132
(Circular) (Six-base) MAPSORT of: pFN21AE1132.seq Check: 7115 from: 1 to: 5629
pFN21AE1132 5629 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 1937
Size: 1035 4594
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2898 3692 5076 5344 1632
Size: 1266 794 1384 268 1917
Fragments arranged by size:
1917 1384 1266 794 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 3372 4944 3372
Size: 1572 4057
ApaI G_GGCC'C
Cuts at: 2092 2092
Size: 5629
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2720 2938 3164 3316 5425 1493
Size: 1227 218 226 152 2109 1697
Fragments arranged by size:
2109 1697 1227 226 218 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2954 1870
Size: 73 142 869 4545
Fragments arranged by size:
4545 869 142 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2692 2950 4405 618
Size: 325 217 627 342 563 258 1455 1842
Fragments arranged by size:
1842 1455 627 563 342 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2034 2092 2160 2948 1391
Size: 643 58 68 788 4072
Fragments arranged by size:
4072 788 643 68 58
BbeI G_GCGC'C
Cuts at: 2133 2133
Size: 5629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2643 3521 4731 2643
Size: 878 1210 3541
Fragments arranged by size:
3541 1210 878
BclI T'GATC_A
Cuts at: 1548 2439 1548
Size: 891 4738
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2192 2564 2855
Size: 107 122 71 631 605 520 372 291
Cuts at: 2855 4240 136
Size: 1385 1525
Fragments arranged by size:
1525 1385 631 605 520 372 291 122
107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2092 3690 4846 1790
Size: 302 1598 1156 2573
Fragments arranged by size:
2573 1598 1156 302
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4318 442
Size: 416 53 83 399 24 1943 958 1753
Fragments arranged by size:
1943 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 4289 1239
Size: 1241 1809 2579
Fragments arranged by size:
2579 1809 1241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 4292 882
Size: 617 576 2217 2219
Fragments arranged by size:
2219 2217 617 576
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 3436 3818 4453
Size: 53 83 186 936 597 1306 382 635
Cuts at: 4453 275
Size: 1451
Fragments arranged by size:
1451 1306 936 635 597 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2156 2477 2480 2483 2486 2489 2492
Size: 949 21 321 3 3 3 3 3
Cuts at: 2492 2505 1186
Size: 13 4310
Fragments arranged by size:
4310 949 321 21 13 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 4832 5328 4832
Size: 496 5133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 2684 2840 1163
Size: 573 540 408 156 3952
Fragments arranged by size:
3952 573 540 408 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 3079 3840 4868 1540
Size: 275 186 96 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186 96
BsiHKAI G_wGCw'C
Cuts at: 3690 3775 4846 3690
Size: 85 1071 4473
Fragments arranged by size:
4473 1071 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2092 2160 2948 3690 3775 4846
Size: 399 244 58 68 788 742 85 1071
Cuts at: 4846 1391
Size: 2174
Fragments arranged by size:
2174 1071 788 742 399 244 85 68
58
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 844
Size: 2140 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 2912 4273 4460 1700
Size: 110 416 686 1361 187 2869
Fragments arranged by size:
2869 1361 686 416 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 3689 4701 3689
Size: 1012 4617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2203 2203
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2482 2959 3326 3711 3728 5180 5266 5278
Size: 510 477 367 385 17 1452 86 12
Cuts at: 5278 5624 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 510 477 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5077
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2341 487
Size: 683 56 1115 3775
Fragments arranged by size:
3775 1115 683 56
BtsI GCAGTG_nn'
Cuts at: 3145 3939 3959 3145
Size: 794 20 4815
Fragments arranged by size:
4815 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2193 2781
Size: 54 1425 50 275 18 238 125 588
Cuts at: 2781 2813 3076 3965 5594 8
Size: 32 263 889 1629 43
Fragments arranged by size:
1629 1425 889 588 275 263 238 125
54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 2633 3559 1353
Size: 926 354 926 3423
Fragments arranged by size:
3423 926 926 354
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 4216 4590 4736 387
Size: 1064 138 672 1955 374 146 1280
Fragments arranged by size:
1955 1280 1064 672 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 2898 3692 4289 5076 5344 1239
Size: 393 848 418 794 597 787 268 1524
Fragments arranged by size:
1524 848 794 787 597 418 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2206 2206
Size: 5629
EcoO109I rG'GnC_Cy
Cuts at: 2089 2184 2217 2732 2833 2089
Size: 95 33 515 101 4885
Fragments arranged by size:
4885 515 101 95 33
EcoRI G'AATT_C
Cuts at: 2720 2938 3316 2720
Size: 218 378 5033
Fragments arranged by size:
5033 378 218
EcoRV GAT'ATC
Cuts at: 2675 2675
Size: 5629
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 2113 2133 2559 4776 1678
Size: 435 20 426 2217 2531
Fragments arranged by size:
2531 2217 435 426 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2572 2604 4270 4302 4344
Size: 32 135 32 1000 32 1666 32 42
Cuts at: 4344 4376 1373
Size: 32 2626
Fragments arranged by size:
2626 1666 1000 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KasI G'GCGC_C
Cuts at: 2129 2129
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4743 4927 1305
Size: 3438 184 2007
Fragments arranged by size:
3438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2783 2815 10
Size: 54 1425 343 951 32 2824
Fragments arranged by size:
2824 1425 951 343 54 32
MslI CAynn'nnrTG
Cuts at: 518 1166 2076 3587 3946 4105 518
Size: 648 910 1511 359 159 2042
Fragments arranged by size:
2042 1511 910 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3045 3722 4870 5115 1606
Size: 1439 677 1148 245 2120
Fragments arranged by size:
2120 1439 1148 677 245
NaeI GCC'GGC
Cuts at: 2228 2914 2228
Size: 686 4943
NarI GG'CG_CC
Cuts at: 2130 2130
Size: 5629
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5077
NdeI CA'TA_TG
Cuts at: 387 2716 387
Size: 2329 3300
NgoMIV G'CCGG_C
Cuts at: 2226 2912 2226
Size: 686 4943
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2987 4532 1132
Size: 97 1758 1545 2229
Fragments arranged by size:
2229 1758 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5629
PfoI T'CCnGG_A
Cuts at: 2346 2346
Size: 5629
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2732 2732
Size: 5629
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5629
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2088 2088
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2981 830
Size: 2151 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SanDI GG'GwC_CC
Cuts at: 2732 2732
Size: 5629
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2977 4112 4793 4984 826
Size: 219 1932 1135 681 191 1471
Fragments arranged by size:
1932 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2192 2855 2192
Size: 663 4966
SfoI GGC'GCC
Cuts at: 2131 2131
Size: 5629
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2087 2956 1872
Size: 215 869 4545
Fragments arranged by size:
4545 869 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5629
StyI C'CwwG_G
Cuts at: 513 1065 2115 2187 2202 2865 3068 513
Size: 552 1050 72 15 663 203 3074
Fragments arranged by size:
3074 1050 663 552 203 72 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3656 3673 3826 4011 4456 325
Size: 1255 2076 17 153 185 445 1498
Fragments arranged by size:
2076 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2085 2954 1870
Size: 215 869 4545
Fragments arranged by size:
4545 869 215
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SrfI
SwaI XcmI