Restriction map for
pFN21AE1122
(Circular) (Six-base) MAPSORT of: pFN21AE1122.seq Check: 3155 from: 1 to: 6004
pFN21AE1122 6004 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2616 2616
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3287 3347 1937
Size: 1350 60 4594
Fragments arranged by size:
4594 1350 60
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2724 2904 4067 5451 5719 1632
Size: 614 478 180 1163 1384 268 1917
Fragments arranged by size:
1917 1384 1163 614 478 268 180
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 3224 1166
Size: 2058 3946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2274 3747 5319 2274
Size: 1473 1572 2959
Fragments arranged by size:
2959 1572 1473
ApaI G_GGCC'C
Cuts at: 2621 2978 2621
Size: 357 5647
ApaLI G'TGCA_C
Cuts at: 2892 4061 5217 2892
Size: 1169 1156 3679
Fragments arranged by size:
3679 1169 1156
ApoI r'AATT_y
Cuts at: 1493 3313 3539 3691 5800 1493
Size: 1820 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2808 3077 3329 1870
Size: 73 865 269 252 4545
Fragments arranged by size:
4545 865 269 252 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 2857 2926 3070 3274
Size: 325 217 627 382 688 69 144 204
Cuts at: 3274 3325 4780 618
Size: 51 1455 1842
Fragments arranged by size:
1842 1455 688 627 382 325 217 204
144 69 51
BanII G_rGCy'C
Cuts at: 1391 2159 2621 2978 3001 3151 3323 1391
Size: 768 462 357 23 150 172 4072
Fragments arranged by size:
4072 768 462 357 172 150 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 2959 928
Size: 971 149 911 3973
Fragments arranged by size:
3973 971 911 149
BbvCI CC'TCA_GC
Cuts at: 3109 3109
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 3157 1548
Size: 1609 4395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2213 4615 136
Size: 107 122 71 631 605 541 2402 1525
Fragments arranged by size:
2402 1525 631 605 541 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 2153 2363 3421 2153
Size: 210 1058 4736
Fragments arranged by size:
4736 1058 210
Bme1580I G_kGCm'C
Cuts at: 1790 2172 2621 2831 2860 2896 2978 3073 4065
Size: 382 449 210 29 36 82 95 992
Cuts at: 4065 5221 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 992 449 382 210 95 82
36 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 3735 4693
Size: 416 53 83 399 24 711 1607 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1607 958 711 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2293 2296 2429 2625 2637 4664 1239
Size: 1054 3 133 196 12 2027 2579
Fragments arranged by size:
2579 2027 1054 196 133 12 3
Bpu10I CC'TnA_GC
Cuts at: 3109 3109
Size: 6004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3122 4129 4994 5292 3122
Size: 1007 865 298 3834
Fragments arranged by size:
3834 1007 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2896 3007 4667 882
Size: 617 1397 111 1660 2219
Fragments arranged by size:
2219 1660 1397 617 111
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2291 2866 3811 4193
Size: 53 83 186 936 758 575 945 382
Cuts at: 4193 4828 275
Size: 635 1451
Fragments arranged by size:
1451 945 936 758 635 575 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2235 4437 4835 5109 5256 5667 2235
Size: 2202 398 274 147 411 2572
Fragments arranged by size:
2572 2202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 1178
Size: 30 165 30 1014 30 4735
Fragments arranged by size:
4735 1014 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 3081 1186
Size: 1210 685 4109
Fragments arranged by size:
4109 1210 685
BseYI C'CCAG_C
Cuts at: 5207 5703 5207
Size: 496 5508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2669 1163
Size: 573 408 525 4498
Fragments arranged by size:
4498 573 525 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2568 2598 2896 4065 4150 5221 2568
Size: 30 298 1169 85 1071 3351
Fragments arranged by size:
3351 1169 1071 298 85 30
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
BsmBI CGTCTCn'nnnn_
Cuts at: 2080 2080
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2172 2568 2598 2621 2831 2860
Size: 399 369 13 396 30 23 210 29
Cuts at: 2860 2896 2978 3001 3073 3151 3323 4065 4150
Size: 36 82 23 72 78 172 742 85
Cuts at: 4150 5221 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 369 210 172
85 82 78 72 36 30 29 23
23 13
BspEI T'CCGG_A
Cuts at: 2235 5667 2235
Size: 3432 2572
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 2592 2616 3359 844
Size: 1643 105 24 743 3489
Fragments arranged by size:
3489 1643 743 105 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2319 3891 1394
Size: 429 496 1572 3507
Fragments arranged by size:
3507 1572 496 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2697 4648 4835 1700
Size: 110 338 549 1951 187 2869
Fragments arranged by size:
2869 1951 549 338 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 2671 2680 794
Size: 1382 495 9 4118
Fragments arranged by size:
4118 1382 495 9
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2781 3024 2422
Size: 359 243 5402
Fragments arranged by size:
5402 359 243
BstYI r'GATC_y
Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999
Size: 1362 367 385 17 1452 86 12 346
Cuts at: 5999 1972
Size: 1977
Fragments arranged by size:
1977 1452 1362 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3288 3288
Size: 6004
Bsu36I CC'TnA_GG
Cuts at: 2625 3093 2625
Size: 468 5536
BtgI C'CryG_G
Cuts at: 513 1065 2004 2506 513
Size: 552 939 502 4011
Fragments arranged by size:
4011 939 552 502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 3147 3162 3520 4314 4334 3147
Size: 15 358 794 20 4817
Fragments arranged by size:
4817 794 358 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969
Size: 54 1425 50 275 18 1621 889 1629
Cuts at: 5969 8
Size: 43
Fragments arranged by size:
1629 1621 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3934 1353
Size: 2581 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2231 4591 4965 5111 387
Size: 1064 138 642 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 642 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2293 2296 2429 2625 2637 2724
Size: 393 614 47 3 133 196 12 87
Cuts at: 2724 2904 4067 4664 5451 5719 1239
Size: 180 1163 597 787 268 1524
Fragments arranged by size:
1524 1163 787 614 597 393 268 196
180 133 87 47 12 3
EcoO109I rG'GnC_Cy
Cuts at: 2242 2629 2938 2974 2975 2242
Size: 387 309 36 1 5271
Fragments arranged by size:
5271 387 309 36 1
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3073 3105 4645 4677 4719
Size: 32 135 32 1501 32 1540 32 42
Cuts at: 4719 4751 1373
Size: 32 2626
Fragments arranged by size:
2626 1540 1501 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3348 3594 669
Size: 988 281 858 552 246 3079
Fragments arranged by size:
3079 988 858 552 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 5118 5302 1305
Size: 1098 2715 184 2007
Fragments arranged by size:
2715 2007 1098 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3224 3962 4321 4480 518
Size: 648 2058 738 359 159 2042
Fragments arranged by size:
2058 2042 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2362 2508 2882 3246 3420 4097 5245
Size: 429 327 146 374 364 174 677 1148
Cuts at: 5245 5490 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 429 374 364 327 245
174 146
NaeI GCC'GGC
Cuts at: 2150 2699 2150
Size: 549 5455
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NgoMIV G'CCGG_C
Cuts at: 2148 2697 2148
Size: 549 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 3362 4907 1132
Size: 97 2133 1545 2229
Fragments arranged by size:
2229 2133 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2614 1348
Size: 1266 4738
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 1108
Size: 1101 4903
PfoI T'CCnGG_A
Cuts at: 2445 3008 2445
Size: 563 5441
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2242
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PspOMI G'GGCC_C
Cuts at: 2617 2974 2617
Size: 357 5647
PstI C_TGCA'G
Cuts at: 830 2040 2553 2664 2881 3356 830
Size: 1210 513 111 217 475 3478
Fragments arranged by size:
3478 1210 513 475 217 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2035 2882 2035
Size: 847 5157
SacII CC_GC'GG
Cuts at: 2509 2509
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SexAI A'CCwGG_T
Cuts at: 2172 2172
Size: 6004
SfcI C'TryA_G
Cuts at: 826 1045 2036 2549 2660 2877 3352 4487 5168
Size: 219 991 513 111 217 475 1135 681
Cuts at: 5168 5359 826
Size: 191 1471
Fragments arranged by size:
1471 1135 991 681 513 475 219 217
191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 2810 3331 1872
Size: 938 521 4545
Fragments arranged by size:
4545 938 521
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3101 4108 5009 5271 820
Size: 197 926 1158 1007 901 262 1553
Fragments arranged by size:
1553 1158 1007 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 2460 2804 3107 1517
Size: 943 344 303 4414
Fragments arranged by size:
4414 943 344 303
StyI C'CwwG_G
Cuts at: 513 1065 2004 2323 2727 3443 513
Size: 552 939 319 404 716 3074
Fragments arranged by size:
3074 939 716 552 404 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 3130 4031 4048 4201 4386 4831
Size: 1255 549 1001 901 17 153 185 445
Cuts at: 4831 325
Size: 1498
Fragments arranged by size:
1498 1255 1001 901 549 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4250 5962 96
Size: 274 80 33 51 1443 2273 1712 138
Fragments arranged by size:
2273 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5964 5996 5964
Size: 32 5972
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2024 2781 2920 2024
Size: 757 139 5108
Fragments arranged by size:
5108 757 139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 2808 3329 1870
Size: 938 521 4545
Fragments arranged by size:
4545 938 521
XmnI GAAnn'nnTTC
Cuts at: 2231 4133 2231
Size: 1902 4102
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI