Restriction map for
pFN21AE1118
(Circular) (Six-base) MAPSORT of: pFN21AE1118.seq Check: 6587 from: 1 to: 5686
pFN21AE1118 5686 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2649 2649
Size: 5686
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3749 5133 5401 1632
Size: 2117 1384 268 1917
Fragments arranged by size:
2117 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 2816 4585 5562 1203
Size: 1613 1769 977 1327
Fragments arranged by size:
1769 1613 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 2422 4415 2422
Size: 1993 3693
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 2078 2560 3429 5001 2078
Size: 482 869 1572 2763
Fragments arranged by size:
2763 1572 869 482
ApaLI G'TGCA_C
Cuts at: 2620 3743 4899 2620
Size: 1123 1156 3407
Fragments arranged by size:
3407 1156 1123
ApoI r'AATT_y
Cuts at: 1493 2603 2995 3221 3373 5482 1493
Size: 1110 392 226 152 2109 1697
Fragments arranged by size:
2109 1697 1110 392 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BamHI G'GATC_C
Cuts at: 2475 3016 3383 2475
Size: 541 367 4778
Fragments arranged by size:
4778 541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 4462 618
Size: 325 217 627 1220 1455 1842
Fragments arranged by size:
1842 1455 1220 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2241 3005 1391
Size: 850 764 4072
Fragments arranged by size:
4072 850 764
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 2937 928
Size: 971 522 516 3677
Fragments arranged by size:
3677 971 522 516
BbvCI CC'TCA_GC
Cuts at: 2912 2912
Size: 5686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 2922 1548
Size: 1374 4312
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2973 4297 136
Size: 107 122 71 631 605 1301 1324 1525
Fragments arranged by size:
1525 1324 1301 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 2963 3103 2963
Size: 140 5546
Bme1580I G_kGCm'C
Cuts at: 1790 2624 3747 4903 1790
Size: 834 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 834
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2489 2744 4346 1239
Size: 1214 36 255 1602 2579
Fragments arranged by size:
2579 1602 1214 255 36
Bpu10I CC'TnA_GC
Cuts at: 2329 2912 2329
Size: 583 5103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 2216 2702 4349 882
Size: 617 582 135 486 1647 2219
Fragments arranged by size:
2219 1647 617 582 486 135
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 3493 3875 4510
Size: 53 83 186 936 783 1177 382 635
Cuts at: 4510 275
Size: 1451
Fragments arranged by size:
1451 1177 936 783 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2526 2556 1178
Size: 30 165 30 1123 30 4308
Fragments arranged by size:
4308 1123 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 2741 1186
Size: 1264 291 4131
Fragments arranged by size:
4131 1264 291
BseYI C'CCAG_C
Cuts at: 4889 5385 4889
Size: 496 5190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2241 2624 3747 3832 4903 2148
Size: 93 383 1123 85 1071 2931
Fragments arranged by size:
2931 1123 1071 383 93 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2241 2624 3005 3747 3832 4903
Size: 399 358 93 383 381 742 85 1071
Cuts at: 4903 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 383 381 358 93
85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2649 3041 844
Size: 1805 392 3489
Fragments arranged by size:
3489 1805 392
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4181 4355 65
Size: 1183 2933 174 1396
Fragments arranged by size:
2933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2358 2475 2557 3016 3383 3768 3785 5237
Size: 386 117 82 459 367 385 17 1452
Cuts at: 5237 5323 5335 5681 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 459 386 385 367 346 117
86 82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2713 513
Size: 552 939 709 3486
Fragments arranged by size:
3486 939 709 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2635 3202 3996 4016 2635
Size: 567 794 20 4305
Fragments arranged by size:
4305 794 567 20
ClaI AT'CG_AT
Cuts at: 1554 2139 1554
Size: 585 5101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2230 4693 809
Size: 507 914 2463 1802
Fragments arranged by size:
2463 1802 914 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3133 4022 5651
Size: 54 1425 50 275 18 1303 889 1629
Cuts at: 5651 8
Size: 43
Fragments arranged by size:
1629 1425 1303 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2652 3616 1353
Size: 692 607 964 3423
Fragments arranged by size:
3423 964 692 607
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4273 4647 4793 387
Size: 1064 138 2684 374 146 1280
Fragments arranged by size:
2684 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2239 2239
Size: 5686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2489 2744 3749 4346 5133 5401
Size: 393 821 36 255 1005 597 787 268
Cuts at: 5401 1239
Size: 1524
Fragments arranged by size:
1524 1005 821 787 597 393 268 255
36
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 2492 2435
Size: 57 5629
EcoO109I rG'GnC_Cy
Cuts at: 2212 2826 2212
Size: 614 5072
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 4833 1678
Size: 3155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2440 2526 2558 4327
Size: 32 135 32 836 32 86 32 1769
Cuts at: 4327 4359 4401 4433 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1769 836 135 86 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 2816 5562 2816
Size: 2746 2940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3864
Fragments arranged by size:
3864 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2744 2924 3644 4003 4162 518
Size: 648 975 603 180 720 359 159 2042
Fragments arranged by size:
2042 975 720 648 603 359 180 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2386 2940 3102 3779 4927 5172 1606
Size: 564 216 554 162 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 554 245 216 162
NcoI C'CATG_G
Cuts at: 513 1065 2004 2713 513
Size: 552 939 709 3486
Fragments arranged by size:
3486 939 709 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4784
PfoI T'CCnGG_A
Cuts at: 2021 2021
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2826 2212
Size: 614 5072
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2496 3038 830
Size: 1666 542 3478
Fragments arranged by size:
3478 1666 542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2386 2386
Size: 5686
SacI G_AGCT'C
Cuts at: 2241 2241
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SanDI GG'GwC_CC
Cuts at: 2826 2826
Size: 5686
SapI GCTCTTCn'nnn_
Cuts at: 2045 2045
Size: 5686
SbfI CC_TGCA'GG
Cuts at: 2496 3038 2496
Size: 542 5144
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 2492 3034 4169 4850 5041 826
Size: 219 1447 542 1135 681 191 1471
Fragments arranged by size:
1471 1447 1135 681 542 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2004 2301 2337 2713 3125 513
Size: 552 939 297 36 376 412 3074
Fragments arranged by size:
3074 939 552 412 376 297 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2771 3713 3730 3883 4068 4513 325
Size: 1255 1191 942 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1191 942 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2740 2740
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI