Restriction map for
pFN21AE1116
(Circular) (Six-base) MAPSORT of: pFN21AE1116.seq Check: 3042 from: 1 to: 5305
pFN21AE1116 5305 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3115 278
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2648 1937
Size: 711 4594
Acc65I G'GTAC_C
Cuts at: 2626 2626
Size: 5305
AclI AA'CG_TT
Cuts at: 1563 3432 3805 1563
Size: 1869 373 3063
Fragments arranged by size:
3063 1869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3368 4752 5020 1632
Size: 1736 1384 268 1917
Fragments arranged by size:
1917 1736 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5108
AflIII A'CryG_T
Cuts at: 1203 4204 5181 1203
Size: 3001 977 1327
Fragments arranged by size:
3001 1327 977
AgeI A'CCGG_T
Cuts at: 4136 4136
Size: 5305
AhdI GACnn_n'nnGTC
Cuts at: 4034 4034
Size: 5305
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5305
AlwNI CAG_nnn'CTG
Cuts at: 3048 4620 3048
Size: 1572 3733
ApaLI G'TGCA_C
Cuts at: 3362 4518 3362
Size: 1156 4149
ApoI r'AATT_y
Cuts at: 1493 2614 2840 2992 5101 1493
Size: 1121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3859 160
Size: 872 2827 1606
Fragments arranged by size:
2827 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2206 2630 1870
Size: 73 142 121 424 4545
Fragments arranged by size:
4545 424 142 121 73
BamHI G'GATC_C
Cuts at: 2635 3002 2635
Size: 367 4938
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2626 4081 618
Size: 325 217 627 839 1455 1842
Fragments arranged by size:
1842 1455 839 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2219 2571 2624 1391
Size: 828 352 53 4072
Fragments arranged by size:
4072 828 352 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 2355 928
Size: 971 132 324 3878
Fragments arranged by size:
3878 971 324 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2380 2414 3496 3530 1903
Size: 34 443 34 1082 34 3678
Fragments arranged by size:
3678 1082 443 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3197 4407 3197
Size: 1210 4095
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2417 3916 136
Size: 107 122 71 631 605 745 1499 1525
Fragments arranged by size:
1525 1499 745 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5300 5300
Size: 5305
BlpI GC'TnA_GC
Cuts at: 2722 2722
Size: 5305
Bme1580I G_kGCm'C
Cuts at: 1790 3366 4522 1790
Size: 1576 1156 2573
Fragments arranged by size:
2573 1576 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2425 3036 3994
Size: 416 53 83 399 24 1008 611 958
Cuts at: 3994 442
Size: 1753
Fragments arranged by size:
1753 1008 958 611 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 3965 1239
Size: 1067 1659 2579
Fragments arranged by size:
2579 1659 1067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 2555 3430 4295 4593 2018
Size: 537 875 865 298 2730
Fragments arranged by size:
2730 875 865 537 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3968 882
Size: 617 2469 2219
Fragments arranged by size:
2469 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2599 3096 493
Size: 711 1395 497 2702
Fragments arranged by size:
2702 1395 711 497
BsaBI GATnn'nnATC
Cuts at: 5299 5299
Size: 5305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3112 3494 4129 275
Size: 53 83 186 936 1579 382 635 1451
Fragments arranged by size:
1579 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2499 3738 4136 4410 4557 4968 2499
Size: 1239 398 274 147 411 2836
Fragments arranged by size:
2836 1239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 1178
Size: 30 165 30 870 30 4180
Fragments arranged by size:
4180 870 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2180 1186
Size: 892 102 4311
Fragments arranged by size:
4311 892 102
BseYI C'CCAG_C
Cuts at: 4508 5004 4508
Size: 496 4809
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4732
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 2755 3516 4544 1540
Size: 275 186 46 708 761 1028 2301
Fragments arranged by size:
2301 1028 761 708 275 186 46
BsiHKAI G_wGCw'C
Cuts at: 2271 3366 3451 4522 2271
Size: 1095 85 1071 3054
Fragments arranged by size:
3054 1095 1071 85
BsmI GAATG_Cn'
Cuts at: 2816 2909 2816
Size: 93 5212
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2271 2571 2624 3366 3451 4522
Size: 399 429 52 300 53 742 85 1071
Cuts at: 4522 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 429 399 300 85 53
52
BspEI T'CCGG_A
Cuts at: 2499 4968 2499
Size: 2469 2836
BspHI T'CATG_A
Cuts at: 3194 4924 3194
Size: 1730 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2660 844
Size: 1816 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3192 1394
Size: 429 1369 3507
Fragments arranged by size:
3507 1369 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3800 3974 65
Size: 1183 2552 174 1396
Fragments arranged by size:
2552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3949 4136 1700
Size: 110 2139 187 2869
Fragments arranged by size:
2869 2139 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5305
BssSI C'ACGA_G
Cuts at: 3365 4377 3365
Size: 1012 4293
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5305
BstBI TT'CG_AA
Cuts at: 2021 4109 2021
Size: 2088 3217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5305
BstYI r'GATC_y
Cuts at: 1972 2635 3002 3387 3404 4856 4942 4954 5300
Size: 663 367 385 17 1452 86 12 346
Cuts at: 5300 1972
Size: 1977
Fragments arranged by size:
1977 1452 663 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2191 2296 2307 513
Size: 552 939 187 105 11 3511
Fragments arranged by size:
3511 939 552 187 105 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4566
Fragments arranged by size:
4566 683 56
BtsI GCAGTG_nn'
Cuts at: 2334 2441 2821 3615 3635 2334
Size: 107 380 794 20 4004
Fragments arranged by size:
4004 794 380 107 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5270
DraI TTT'AAA
Cuts at: 2609 2609
Size: 5305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2110 4312 809
Size: 507 794 2202 1802
Fragments arranged by size:
2202 1802 794 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2752 3641 5270
Size: 54 1425 50 275 18 922 889 1629
Cuts at: 5270 8
Size: 43
Fragments arranged by size:
1629 1425 922 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2752 1537
Size: 275 940 4090
Fragments arranged by size:
4090 940 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3235 1353
Size: 1882 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3892 4266 4412 387
Size: 1064 138 2303 374 146 1280
Fragments arranged by size:
2303 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 3368 3965 4752 5020 1239
Size: 393 674 1062 597 787 268 1524
Fragments arranged by size:
1524 1062 787 674 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2540
Size: 5305
EcoO109I rG'GnC_Cy
Cuts at: 2089 2089
Size: 5305
EcoRI G'AATT_C
Cuts at: 2614 2992 2614
Size: 378 4927
FspI TGC'GCA
Cuts at: 3811 3811
Size: 5305
HaeII r_GCGC'y
Cuts at: 1678 2581 4452 1678
Size: 903 1871 2531
Fragments arranged by size:
2531 1871 903
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 3946 3978 4020
Size: 32 135 32 752 32 1590 32 42
Cuts at: 4020 4052 1373
Size: 32 2626
Fragments arranged by size:
2626 1590 752 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 2895 669
Size: 988 281 711 246 3079
Fragments arranged by size:
3079 988 711 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5305
HpaI GTT'AAC
Cuts at: 2895 2895
Size: 5305
KpnI G_GTAC'C
Cuts at: 2630 2630
Size: 5305
MfeI C'AATT_G
Cuts at: 2904 2904
Size: 5305
MluI A'CGCG_T
Cuts at: 5181 5181
Size: 5305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 2202 4419 4603 1305
Size: 762 135 2217 184 2007
Fragments arranged by size:
2217 2007 762 184 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3483
Fragments arranged by size:
3483 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3263 3622 3781 518
Size: 648 2097 359 159 2042
Fragments arranged by size:
2097 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2137 2193 2298 2721 3398 4546 4791
Size: 519 12 56 105 423 677 1148 245
Cuts at: 4791 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 519 423 245 105 56
12
NcoI C'CATG_G
Cuts at: 513 1065 2004 2307 513
Size: 552 939 303 3511
Fragments arranged by size:
3511 939 552 303
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5305
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5305
NotI GC'GGCC_GC
Cuts at: 2752 2752
Size: 5305
NspI r_CATG'y
Cuts at: 1132 1229 2134 2401 2663 4208 1132
Size: 97 905 267 262 1545 2229
Fragments arranged by size:
2229 1545 905 267 262 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5305
PciI A'CATG_T
Cuts at: 4204 4204
Size: 5305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5305
PmeI GTTT'AAAC
Cuts at: 2609 2609
Size: 5305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 4887 4919 3374
Size: 32 1481 32 3760
Fragments arranged by size:
3760 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2089
Size: 5305
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5305
PsiI TTA'TAA
Cuts at: 2875 2875
Size: 5305
PstI C_TGCA'G
Cuts at: 830 2553 2657 830
Size: 1723 104 3478
Fragments arranged by size:
3478 1723 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5305
PvuII CAG'CTG
Cuts at: 2125 2125
Size: 5305
SacII CC_GC'GG
Cuts at: 2194 2299 2194
Size: 105 5200
SalI G'TCGA_C
Cuts at: 1936 2647 1936
Size: 711 4594
SbfI CC_TGCA'GG
Cuts at: 2657 2657
Size: 5305
ScaI AGT'ACT
Cuts at: 3553 5265 3553
Size: 1712 3593
SfcI C'TryA_G
Cuts at: 826 1045 2549 2653 3788 4469 4660 826
Size: 219 1504 104 1135 681 191 1471
Fragments arranged by size:
1504 1471 1135 681 219 191 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5305
SmaI CCC'GGG
Cuts at: 1872 2208 2632 1872
Size: 336 424 4545
Fragments arranged by size:
4545 424 336
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 2570 3409 4310 4572 820
Size: 197 926 90 537 839 901 262 1553
Fragments arranged by size:
1553 926 901 839 537 262 197 90
SnaBI TAC'GTA
Cuts at: 493 3096 493
Size: 2603 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5305
SphI G_CATG'C
Cuts at: 1132 2663 1132
Size: 1531 3774
SspI AAT'ATT
Cuts at: 5 52 2386 3229 5
Size: 47 2334 843 2081
Fragments arranged by size:
2334 2081 843 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5305
StyI C'CwwG_G
Cuts at: 513 1065 2004 2050 2307 2744 513
Size: 552 939 46 257 437 3074
Fragments arranged by size:
3074 939 552 437 257 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3332 3349 3502 3687 4132 325
Size: 1255 1752 17 153 185 445 1498
Fragments arranged by size:
1752 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3551 5263 96
Size: 274 80 33 51 1443 1574 1712 138
Fragments arranged by size:
1712 1574 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5265 5297 5265
Size: 32 5273
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5305
XbaI T'CTAG_A
Cuts at: 2641 2641
Size: 5305
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5305
XmaI C'CCGG_G
Cuts at: 1870 2206 2630 1870
Size: 336 424 4545
Fragments arranged by size:
4545 424 336
XmnI GAAnn'nnTTC
Cuts at: 3434 3434
Size: 5305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3113 276
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI