Restriction map for
pFN21AE1109
(Circular) (Six-base) MAPSORT of: pFN21AE1109.seq Check: 5648 from: 1 to: 5038
pFN21AE1109 5038 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2306 2306
Size: 5038
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2848 278
Size: 53 83 186 936 1312 2468
Fragments arranged by size:
2468 1312 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2381 1937
Size: 444 4594
Acc65I G'GTAC_C
Cuts at: 2359 2359
Size: 5038
AclI AA'CG_TT
Cuts at: 1563 3165 3538 1563
Size: 1602 373 3063
Fragments arranged by size:
3063 1602 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3101 4485 4753 1632
Size: 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4841
AflIII A'CryG_T
Cuts at: 1203 3937 4914 1203
Size: 2734 977 1327
Fragments arranged by size:
2734 1327 977
AgeI A'CCGG_T
Cuts at: 3869 3869
Size: 5038
AhdI GACnn_n'nnGTC
Cuts at: 3767 3767
Size: 5038
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5038
AlwNI CAG_nnn'CTG
Cuts at: 2781 4353 2781
Size: 1572 3466
ApaLI G'TGCA_C
Cuts at: 3095 4251 3095
Size: 1156 3882
ApoI r'AATT_y
Cuts at: 1493 2347 2573 2725 4834 1493
Size: 854 226 152 2109 1697
Fragments arranged by size:
2109 1697 854 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3592 160
Size: 872 2560 1606
Fragments arranged by size:
2560 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5038
AvaI C'yCGr_G
Cuts at: 1870 1943 2363 1870
Size: 73 420 4545
Fragments arranged by size:
4545 420 73
AvrII C'CTAG_G
Cuts at: 2036 2036
Size: 5038
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2250 2283 2250
Size: 33 5005
BamHI G'GATC_C
Cuts at: 2368 2735 2368
Size: 367 4671
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2359 3814 618
Size: 325 217 627 572 1455 1842
Fragments arranged by size:
1842 1455 627 572 325 217
BanII G_rGCy'C
Cuts at: 1391 2357 1391
Size: 966 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3229 3263 1903
Size: 34 1292 34 3678
Fragments arranged by size:
3678 1292 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2930 4140 2930
Size: 1210 3828
BclI T'GATC_A
Cuts at: 1548 2149 2167 1548
Size: 601 18 4419
Fragments arranged by size:
4419 601 18
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3649 136
Size: 107 122 71 631 605 1977 1525
Fragments arranged by size:
1977 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5033 5033
Size: 5038
BlpI GC'TnA_GC
Cuts at: 2455 2455
Size: 5038
Bme1580I G_kGCm'C
Cuts at: 1790 3099 4255 1790
Size: 1309 1156 2573
Fragments arranged by size:
2573 1309 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2247 2769 3727
Size: 416 53 83 399 24 830 522 958
Cuts at: 3727 442
Size: 1753
Fragments arranged by size:
1753 958 830 522 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 3698 1239
Size: 959 1500 2579
Fragments arranged by size:
2579 1500 959
Bpu10I CC'TnA_GC
Cuts at: 2064 2064
Size: 5038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3163 4028 4326 3163
Size: 865 298 3875
Fragments arranged by size:
3875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3701 882
Size: 617 2202 2219
Fragments arranged by size:
2219 2202 617
BsaAI yAC'GTr
Cuts at: 493 1204 2829 493
Size: 711 1625 2702
Fragments arranged by size:
2702 1625 711
BsaBI GATnn'nnATC
Cuts at: 5032 5032
Size: 5038
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2845 3227 3862 275
Size: 53 83 186 936 1312 382 635 1451
Fragments arranged by size:
1451 1312 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3471 3869 4143 4290 4701 3471
Size: 398 274 147 411 3808
Fragments arranged by size:
3808 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4813
Fragments arranged by size:
4813 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5038
BseYI C'CCAG_C
Cuts at: 4241 4737 4241
Size: 496 4542
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2196 1163
Size: 573 460 4005
Fragments arranged by size:
4005 573 460
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2488 3249 4277 1540
Size: 275 186 487 761 1028 2301
Fragments arranged by size:
2301 1028 761 487 275 186
BsiHKAI G_wGCw'C
Cuts at: 3099 3184 4255 3099
Size: 85 1071 3882
Fragments arranged by size:
3882 1071 85
BsmI GAATG_Cn'
Cuts at: 2549 2642 2549
Size: 93 4945
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 3099 3184 4255 1391
Size: 399 567 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 85
BspEI T'CCGG_A
Cuts at: 4701 4701
Size: 5038
BspHI T'CATG_A
Cuts at: 2927 4657 2927
Size: 1730 3308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 2393 844
Size: 1462 87 3489
Fragments arranged by size:
3489 1462 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2925 1394
Size: 429 1102 3507
Fragments arranged by size:
3507 1102 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3533 3707 65
Size: 1183 2285 174 1396
Fragments arranged by size:
2285 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3682 3869 1700
Size: 110 1872 187 2869
Fragments arranged by size:
2869 1872 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5038
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5038
BssSI C'ACGA_G
Cuts at: 3098 4110 3098
Size: 1012 4026
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5038
BstBI TT'CG_AA
Cuts at: 3842 3842
Size: 5038
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5038
BstYI r'GATC_y
Cuts at: 1972 2368 2735 3120 3137 4589 4675 4687 5033
Size: 396 367 385 17 1452 86 12 346
Cuts at: 5033 1972
Size: 1977
Fragments arranged by size:
1977 1452 396 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4299
Fragments arranged by size:
4299 683 56
BtsI GCAGTG_nn'
Cuts at: 2554 3348 3368 2554
Size: 794 20 4224
Fragments arranged by size:
4224 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5003
DraI TTT'AAA
Cuts at: 2302 2342 2302
Size: 40 4998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4045 809
Size: 507 2729 1802
Fragments arranged by size:
2729 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2485 3374 5003
Size: 54 1425 50 275 18 655 889 1629
Cuts at: 5003 8
Size: 43
Fragments arranged by size:
1629 1425 889 655 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2485 1537
Size: 275 673 4090
Fragments arranged by size:
4090 673 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2106 2150 2968 1353
Size: 741 12 44 818 3423
Fragments arranged by size:
3423 818 741 44 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3625 3999 4145 387
Size: 1064 138 2036 374 146 1280
Fragments arranged by size:
2036 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 3101 3698 4485 4753 1239
Size: 393 566 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 566 393 268
EcoRI G'AATT_C
Cuts at: 2347 2725 2347
Size: 378 4660
FspI TGC'GCA
Cuts at: 3544 3544
Size: 5038
HaeII r_GCGC'y
Cuts at: 1678 4185 1678
Size: 2507 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3679 3711 3753 3785 1373
Size: 32 135 32 2107 32 42 32 2626
Fragments arranged by size:
2626 2107 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2382 2628 669
Size: 988 281 444 246 3079
Fragments arranged by size:
3079 988 444 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5038
HpaI GTT'AAC
Cuts at: 2628 2628
Size: 5038
KpnI G_GTAC'C
Cuts at: 2363 2363
Size: 5038
MfeI C'AATT_G
Cuts at: 2637 2637
Size: 5038
MluI A'CGCG_T
Cuts at: 4914 4914
Size: 5038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2114 4152 4336 1305
Size: 809 2038 184 2007
Fragments arranged by size:
2038 2007 809 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3216
Fragments arranged by size:
3216 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2996 3355 3514 518
Size: 648 1830 359 159 2042
Fragments arranged by size:
2042 1830 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2454 3131 4279 4524 1606
Size: 574 274 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 574 274 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4486
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5038
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5038
NotI GC'GGCC_GC
Cuts at: 2485 2485
Size: 5038
NspI r_CATG'y
Cuts at: 1132 1229 2396 3941 1132
Size: 97 1167 1545 2229
Fragments arranged by size:
2229 1545 1167 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5038
PciI A'CATG_T
Cuts at: 3937 3937
Size: 5038
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4136
PfoI T'CCnGG_A
Cuts at: 2214 2214
Size: 5038
PmeI GTTT'AAAC
Cuts at: 2342 2342
Size: 5038
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 4620 4652 3107
Size: 32 1481 32 3493
Fragments arranged by size:
3493 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5038
PsiI TTA'TAA
Cuts at: 2608 2608
Size: 5038
PstI C_TGCA'G
Cuts at: 830 2390 830
Size: 1560 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5038
PvuII CAG'CTG
Cuts at: 2180 2180
Size: 5038
SalI G'TCGA_C
Cuts at: 1936 2380 1936
Size: 444 4594
SapI GCTCTTCn'nnn_
Cuts at: 2094 2094
Size: 5038
SbfI CC_TGCA'GG
Cuts at: 2390 2390
Size: 5038
ScaI AGT'ACT
Cuts at: 3286 4998 3286
Size: 1712 3326
SfcI C'TryA_G
Cuts at: 826 1045 2386 3521 4202 4393 826
Size: 219 1341 1135 681 191 1471
Fragments arranged by size:
1471 1341 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5038
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5038
SmaI CCC'GGG
Cuts at: 1872 2365 1872
Size: 493 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3142 4043 4305 820
Size: 197 926 1199 901 262 1553
Fragments arranged by size:
1553 1199 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2829 493
Size: 2336 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5038
SphI G_CATG'C
Cuts at: 1132 2396 1132
Size: 1264 3774
SspI AAT'ATT
Cuts at: 5 52 2962 5
Size: 47 2910 2081
Fragments arranged by size:
2910 2081 47
StuI AGG'CCT
Cuts at: 1517 2035 1517
Size: 518 4520
StyI C'CwwG_G
Cuts at: 513 1065 2036 2477 513
Size: 552 971 441 3074
Fragments arranged by size:
3074 971 552 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 3065 3082 3235 3420 3865 325
Size: 1255 557 928 17 153 185 445 1498
Fragments arranged by size:
1498 1255 928 557 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3284 4996 96
Size: 274 80 33 51 1443 1307 1712 138
Fragments arranged by size:
1712 1443 1307 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4998 5030 4998
Size: 32 5006
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5038
XbaI T'CTAG_A
Cuts at: 2374 2374
Size: 5038
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5038
XmaI C'CCGG_G
Cuts at: 1870 2363 1870
Size: 493 4545
XmnI GAAnn'nnTTC
Cuts at: 3167 3167
Size: 5038
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2846 276
Size: 53 83 186 936 1312 2468
Fragments arranged by size:
2468 1312 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI