Restriction map for
pFN21AE1104
(Circular) (Six-base) MAPSORT of: pFN21AE1104.seq Check: 3728 from: 1 to: 5188
pFN21AE1104 5188 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2998 278
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2531 1937
Size: 594 4594
Acc65I G'GTAC_C
Cuts at: 2280 2509 2280
Size: 229 4959
AclI AA'CG_TT
Cuts at: 1563 3315 3688 1563
Size: 1752 373 3063
Fragments arranged by size:
3063 1752 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3251 4635 4903 1632
Size: 1619 1384 268 1917
Fragments arranged by size:
1917 1619 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4991
AflIII A'CryG_T
Cuts at: 1203 4087 5064 1203
Size: 2884 977 1327
Fragments arranged by size:
2884 1327 977
AgeI A'CCGG_T
Cuts at: 4019 4019
Size: 5188
AhdI GACnn_n'nnGTC
Cuts at: 3917 3917
Size: 5188
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2228 2354 1184
Size: 1044 126 4018
Fragments arranged by size:
4018 1044 126
AlwNI CAG_nnn'CTG
Cuts at: 2341 2931 4503 2341
Size: 590 1572 3026
Fragments arranged by size:
3026 1572 590
ApaI G_GGCC'C
Cuts at: 2060 2060
Size: 5188
ApaLI G'TGCA_C
Cuts at: 3245 4401 3245
Size: 1156 4032
ApoI r'AATT_y
Cuts at: 1493 2497 2723 2875 4984 1493
Size: 1004 226 152 2109 1697
Fragments arranged by size:
2109 1697 1004 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3742 160
Size: 872 2710 1606
Fragments arranged by size:
2710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 1870
Size: 73 570 4545
Fragments arranged by size:
4545 570 73
BamHI G'GATC_C
Cuts at: 2518 2885 2518
Size: 367 4821
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2280 2509 3964 618
Size: 325 217 627 493 229 1455 1842
Fragments arranged by size:
1842 1455 627 493 325 229 217
BanII G_rGCy'C
Cuts at: 1391 2060 2190 2507 1391
Size: 669 130 317 4072
Fragments arranged by size:
4072 669 317 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4217
BbvCI CC'TCA_GC
Cuts at: 2276 2430 2276
Size: 154 5034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2138 2172 3379 3413 1903
Size: 34 201 34 1207 34 3678
Fragments arranged by size:
3678 1207 201 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3080 4290 3080
Size: 1210 3978
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5188
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2216 2247 3799
Size: 107 122 71 631 605 544 31 1552
Cuts at: 3799 136
Size: 1525
Fragments arranged by size:
1552 1525 631 605 544 122 107 71
31
BglII A'GATC_T
Cuts at: 5183 5183
Size: 5188
BlpI GC'TnA_GC
Cuts at: 2605 2605
Size: 5188
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2212 3249 4405 1790
Size: 270 152 1037 1156 2573
Fragments arranged by size:
2573 1156 1037 270 152
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2919 3877 442
Size: 416 53 83 399 24 1502 958 1753
Fragments arranged by size:
1753 1502 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2204 2216 2306 2390 3848 1239
Size: 887 78 12 90 84 1458 2579
Fragments arranged by size:
2579 1458 887 90 84 78 12
Bpu10I CC'TnA_GC
Cuts at: 2237 2276 2300 2430 2237
Size: 39 24 130 4995
Fragments arranged by size:
4995 130 39 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 3313 4178 4476 2213
Size: 1100 865 298 2925
Fragments arranged by size:
2925 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2323 3851 882
Size: 617 824 1528 2219
Fragments arranged by size:
2219 1528 824 617
BsaAI yAC'GTr
Cuts at: 493 1204 2979 493
Size: 711 1775 2702
Fragments arranged by size:
2702 1775 711
BsaBI GATnn'nnATC
Cuts at: 5182 5182
Size: 5188
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2995 3377 4012
Size: 53 83 186 936 600 862 382 635
Cuts at: 4012 275
Size: 1451
Fragments arranged by size:
1451 936 862 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3621 4019 4293 4440 4851 3621
Size: 398 274 147 411 3958
Fragments arranged by size:
3958 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2303 2333 1178
Size: 30 165 30 900 30 4033
Fragments arranged by size:
4033 900 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5188
BseYI C'CCAG_C
Cuts at: 2053 4391 4887 2053
Size: 2338 496 2354
Fragments arranged by size:
2354 2338 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4615
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2638 3399 4427 1540
Size: 275 186 637 761 1028 2301
Fragments arranged by size:
2301 1028 761 637 275 186
BsiHKAI G_wGCw'C
Cuts at: 2306 3249 3334 4405 2306
Size: 943 85 1071 3089
Fragments arranged by size:
3089 1071 943 85
BsmI GAATG_Cn'
Cuts at: 2699 2792 2699
Size: 93 5095
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2190 2212 2306 2507 3249 3334
Size: 399 270 130 22 94 201 742 85
Cuts at: 3334 4405 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 270 201 130 94
85 22
BspEI T'CCGG_A
Cuts at: 4851 4851
Size: 5188
BspHI T'CATG_A
Cuts at: 3077 4807 3077
Size: 1730 3458
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2281 2543 844
Size: 1437 262 3489
Fragments arranged by size:
3489 1437 262
BsrBI CCG'CTC
Cuts at: 1394 1823 3075 1394
Size: 429 1252 3507
Fragments arranged by size:
3507 1252 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3683 3857 65
Size: 1183 2435 174 1396
Fragments arranged by size:
2435 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3832 4019 1700
Size: 110 2022 187 2869
Fragments arranged by size:
2869 2022 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5188
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5188
BssSI C'ACGA_G
Cuts at: 3248 4260 3248
Size: 1012 4176
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5188
BstBI TT'CG_AA
Cuts at: 3992 3992
Size: 5188
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5188
BstYI r'GATC_y
Cuts at: 1972 2518 2885 3270 3287 4739 4825 4837 5183
Size: 546 367 385 17 1452 86 12 346
Cuts at: 5183 1972
Size: 1977
Fragments arranged by size:
1977 1452 546 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2424 2424
Size: 5188
BtgI C'CryG_G
Cuts at: 513 1065 2004 2084 513
Size: 552 939 80 3617
Fragments arranged by size:
3617 939 552 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4449
Fragments arranged by size:
4449 683 56
BtsI GCAGTG_nn'
Cuts at: 2212 2704 3498 3518 2212
Size: 492 794 20 3882
Fragments arranged by size:
3882 794 492 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5153
DraI TTT'AAA
Cuts at: 2492 2492
Size: 5188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4195 809
Size: 507 2879 1802
Fragments arranged by size:
2879 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2635 3524 5153
Size: 54 1425 50 275 18 805 889 1629
Cuts at: 5153 8
Size: 43
Fragments arranged by size:
1629 1425 889 805 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2635 1537
Size: 275 823 4090
Fragments arranged by size:
4090 823 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3118 1353
Size: 1765 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3775 4149 4295 387
Size: 1064 138 2186 374 146 1280
Fragments arranged by size:
2186 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2204 2216 2306 2390 3251 3848
Size: 393 494 78 12 90 84 861 597
Cuts at: 3848 4635 4903 1239
Size: 787 268 1524
Fragments arranged by size:
1524 861 787 597 494 393 268 90
84 78 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2481 2288
Size: 193 4995
EcoO109I rG'GnC_Cy
Cuts at: 2233 2293 2233
Size: 60 5128
EcoRI G'AATT_C
Cuts at: 2497 2875 2497
Size: 378 4810
FspI TGC'GCA
Cuts at: 3694 3694
Size: 5188
HaeII r_GCGC'y
Cuts at: 1678 4335 1678
Size: 2657 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3829 3861 3903 3935 1373
Size: 32 135 32 2257 32 42 32 2626
Fragments arranged by size:
2626 2257 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 2532 2778 669
Size: 988 281 373 221 246 3079
Fragments arranged by size:
3079 988 373 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5188
HpaI GTT'AAC
Cuts at: 2778 2778
Size: 5188
KpnI G_GTAC'C
Cuts at: 2284 2513 2284
Size: 229 4959
MfeI C'AATT_G
Cuts at: 2787 2787
Size: 5188
MluI A'CGCG_T
Cuts at: 5064 5064
Size: 5188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4302 4486 1305
Size: 2997 184 2007
Fragments arranged by size:
2997 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3366
Fragments arranged by size:
3366 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 3146 3505 3664 518
Size: 648 1170 810 359 159 2042
Fragments arranged by size:
2042 1170 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2604 3281 4429 4674 1606
Size: 540 458 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 458 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3697
Fragments arranged by size:
3697 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5188
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5188
NotI GC'GGCC_GC
Cuts at: 2635 2635
Size: 5188
NspI r_CATG'y
Cuts at: 1132 1229 2546 4091 1132
Size: 97 1317 1545 2229
Fragments arranged by size:
2229 1545 1317 97
PasI CC'CwG_GG
Cuts at: 1348 2461 1348
Size: 1113 4075
PciI A'CATG_T
Cuts at: 4087 4087
Size: 5188
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 1108
Size: 1233 3955
PmeI GTTT'AAAC
Cuts at: 2492 2492
Size: 5188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 4770 4802 3257
Size: 32 1481 32 3643
Fragments arranged by size:
3643 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2293
Size: 5188
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5188
PsiI TTA'TAA
Cuts at: 2758 2758
Size: 5188
PspOMI G'GGCC_C
Cuts at: 2056 2056
Size: 5188
PstI C_TGCA'G
Cuts at: 830 2094 2151 2540 830
Size: 1264 57 389 3478
Fragments arranged by size:
3478 1264 389 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5188
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 5188
SalI G'TCGA_C
Cuts at: 1936 2530 1936
Size: 594 4594
SbfI CC_TGCA'GG
Cuts at: 2540 2540
Size: 5188
ScaI AGT'ACT
Cuts at: 3436 5148 3436
Size: 1712 3476
SfcI C'TryA_G
Cuts at: 826 1045 2090 2147 2536 3671 4352 4543 826
Size: 219 1045 57 389 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 681 389 219 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5188
SmaI CCC'GGG
Cuts at: 1872 2515 1872
Size: 643 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 3292 4193 4455 820
Size: 197 926 285 1064 901 262 1553
Fragments arranged by size:
1553 1064 926 901 285 262 197
SnaBI TAC'GTA
Cuts at: 493 2979 493
Size: 2486 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5188
SphI G_CATG'C
Cuts at: 1132 2546 1132
Size: 1414 3774
SspI AAT'ATT
Cuts at: 5 52 3112 5
Size: 47 3060 2081
Fragments arranged by size:
3060 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5188
StyI C'CwwG_G
Cuts at: 513 1065 2004 2627 513
Size: 552 939 623 3074
Fragments arranged by size:
3074 939 623 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3215 3232 3385 3570 4015 325
Size: 1255 1635 17 153 185 445 1498
Fragments arranged by size:
1635 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3434 5146 96
Size: 274 80 33 51 1443 1457 1712 138
Fragments arranged by size:
1712 1457 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5148 5180 5148
Size: 32 5156
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5188
XbaI T'CTAG_A
Cuts at: 2524 2524
Size: 5188
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5188
XmaI C'CCGG_G
Cuts at: 1870 2513 1870
Size: 643 4545
XmnI GAAnn'nnTTC
Cuts at: 3317 3317
Size: 5188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2996 276
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI