Restriction map for
pFN21AE1097
(Circular) (Six-base) MAPSORT of: pFN21AE1097.seq Check: 9637 from: 1 to: 6523
pFN21AE1097 6523 bp
With 182 enzymes: SgfI *
November 19, 2009 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4333 278
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3866 1937
Size: 1929 4594
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2773 2972 3238 4586 5970 6238 1632
Size: 1010 131 199 266 1348 1384 268 1917
Fragments arranged by size:
1917 1384 1348 1010 268 266 199 131
AfeI AGC'GCT
Cuts at: 3251 3251
Size: 6523
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 5422 6399 1203
Size: 4219 977 1327
Fragments arranged by size:
4219 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 2165 1166
Size: 999 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3747 3779 3747
Size: 32 6491
AlwNI CAG_nnn'CTG
Cuts at: 2136 2326 2428 2557 4266 5838 2136
Size: 190 102 129 1709 1572 2821
Fragments arranged by size:
2821 1709 1572 190 129 102
ApaI G_GGCC'C
Cuts at: 2124 3476 3539 3639 3657 3675 2124
Size: 1352 63 100 18 18 4972
Fragments arranged by size:
4972 1352 100 63 18 18
ApaLI G'TGCA_C
Cuts at: 2663 3116 4580 5736 2663
Size: 453 1464 1156 3450
Fragments arranged by size:
3450 1464 1156 453
ApoI r'AATT_y
Cuts at: 1493 2418 3832 4058 4210 6319 1493
Size: 925 1414 226 152 2109 1697
Fragments arranged by size:
2109 1697 1414 925 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 3549 3658 3784 3848 1870
Size: 73 1606 109 126 64 4545
Fragments arranged by size:
4545 1606 126 109 73 64
BamHI G'GATC_C
Cuts at: 2329 2703 2712 3762 3853 4220 2329
Size: 374 9 1050 91 367 4632
Fragments arranged by size:
4632 1050 374 367 91 9
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3077 3227 3374 3844 5299
Size: 325 217 627 1290 150 147 470 1455
Cuts at: 5299 618
Size: 1842
Fragments arranged by size:
1842 1455 1290 627 470 325 217 150
147
BanII G_rGCy'C
Cuts at: 1391 2124 2565 3192 3476 3539 3639 3657 3675
Size: 733 441 627 284 63 100 18 18
Cuts at: 3675 3842 1391
Size: 167 4072
Fragments arranged by size:
4072 733 627 441 284 167 100 63
18 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2265 2729 3716 928
Size: 971 366 464 987 3735
Fragments arranged by size:
3735 987 971 464 366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2018 2052 4714 4748 1903
Size: 34 81 34 2662 34 3678
Fragments arranged by size:
3678 2662 81 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4415 5625 4415
Size: 1210 5313
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6523
BfrBI ATG'CAT
Cuts at: 2980 2980
Size: 6523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3543 3570 3643
Size: 107 122 71 631 605 1871 27 73
Cuts at: 3643 3652 3679 5134 136
Size: 9 27 1455 1525
Fragments arranged by size:
1871 1525 1455 631 605 122 107 73
71 27 27 9
BglII A'GATC_T
Cuts at: 6518 6518
Size: 6523
BlpI GC'TnA_GC
Cuts at: 3940 3940
Size: 6523
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2667 3120 3230 3271 3379 3476 3539
Size: 334 543 453 110 41 108 97 63
Cuts at: 3539 3639 3657 3675 4584 5740 1790
Size: 100 18 18 909 1156 2573
Fragments arranged by size:
2573 1156 909 543 453 334 110 108
100 97 63 41 18 18
BmgBI CAC'GTC
Cuts at: 1318 3145 1318
Size: 1827 4696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 4254 5212
Size: 416 53 83 399 24 852 1985 958
Cuts at: 5212 442
Size: 1753
Fragments arranged by size:
1985 1753 958 852 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2432 2732 2841 2894 3372 3528 3615
Size: 1064 129 300 109 53 478 156 87
Cuts at: 3615 3651 3777 5183 1239
Size: 36 126 1406 2579
Fragments arranged by size:
2579 1406 1064 478 300 156 129 126
109 87 53 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3113 4648 5513 5811 3113
Size: 1535 865 298 3825
Fragments arranged by size:
3825 1535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2729 3450 3669 3785 5186 882
Size: 617 1230 721 219 116 1401 2219
Fragments arranged by size:
2219 1401 1230 721 617 219 116
BsaAI yAC'GTr
Cuts at: 493 1204 3115 4314 493
Size: 711 1911 1199 2702
Fragments arranged by size:
2702 1911 1199 711
BsaBI GATnn'nnATC
Cuts at: 6517 6517
Size: 6523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4330 4712 5347 275
Size: 53 83 186 936 2797 382 635 1451
Fragments arranged by size:
2797 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3288 4956 5354 5628 5775 6186 3288
Size: 1668 398 274 147 411 3625
Fragments arranged by size:
3625 1668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2814 2844 1178
Size: 30 165 30 834 30 547 30 4857
Fragments arranged by size:
4857 834 547 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2747 2851 3200 3326 1186
Size: 1234 327 104 349 126 4383
Fragments arranged by size:
4383 1234 349 327 126 104
BseYI C'CCAG_C
Cuts at: 2369 2377 3163 3223 5726 6222 2369
Size: 8 786 60 2503 496 2670
Fragments arranged by size:
2670 2503 786 496 60 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2468 3142 1163
Size: 573 732 674 4544
Fragments arranged by size:
4544 732 674 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 3973 4734 5762 1540
Size: 275 186 21 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186 21
BsiHKAI G_wGCw'C
Cuts at: 2484 2667 3120 3192 4584 4669 5740 2484
Size: 183 453 72 1392 85 1071 3267
Fragments arranged by size:
3267 1392 1071 453 183 85 72
BsmI GAATG_Cn'
Cuts at: 4034 4127 4034
Size: 93 6430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2484 2565 2667 3120 3192 3230
Size: 399 334 360 81 102 453 72 38
Cuts at: 3230 3271 3379 3476 3539 3639 3657 3675 3842
Size: 41 108 97 63 100 18 18 167
Cuts at: 3842 4584 4669 5740 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 360 334 167
108 102 100 97 85 81 72 63
41 38 18 18
BspEI T'CCGG_A
Cuts at: 3288 6186 3288
Size: 2898 3625
BspHI T'CATG_A
Cuts at: 4412 6142 4412
Size: 1730 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3246 3878 844
Size: 2402 632 3489
Fragments arranged by size:
3489 2402 632
BsrBI CCG'CTC
Cuts at: 1394 1823 4410 1394
Size: 429 2587 3507
Fragments arranged by size:
3507 2587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3361 5018 5192 65
Size: 1183 2113 1657 174 1396
Fragments arranged by size:
2113 1657 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2112 5167 5354 1700
Size: 110 211 91 3055 187 2869
Fragments arranged by size:
3055 2869 211 187 110 91
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 3062 4583 5595 3062
Size: 1521 1012 3990
Fragments arranged by size:
3990 1521 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2136 3124 794
Size: 1342 988 4193
Fragments arranged by size:
4193 1342 988
BstBI TT'CG_AA
Cuts at: 5327 5327
Size: 6523
BstEII G'GTnAC_C
Cuts at: 1184 3717 1184
Size: 2533 3990
BstXI CCAn_nnnn'nTGG
Cuts at: 2369 3223 3307 2369
Size: 854 84 5585
Fragments arranged by size:
5585 854 84
BstYI r'GATC_y
Cuts at: 1972 2329 2388 2703 2712 3762 3853 4220 4605
Size: 357 59 315 9 1050 91 367 385
Cuts at: 4605 4622 6074 6160 6172 6518 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1050 385 367 357 346 315
91 86 59 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 3495 3676 3684 3747 3495
Size: 181 8 63 6271
Fragments arranged by size:
6271 181 63 8
BtgI C'CryG_G
Cuts at: 513 1065 2208 2682 2707 3738 513
Size: 552 1143 474 25 1031 3298
Fragments arranged by size:
3298 1143 1031 552 474 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2043 487
Size: 683 56 817 4967
Fragments arranged by size:
4967 817 683 56
BtsI GCAGTG_nn'
Cuts at: 2667 3330 4039 4833 4853 2667
Size: 663 709 794 20 4337
Fragments arranged by size:
4337 794 709 663 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3284 3489 5530 809
Size: 507 1968 205 2041 1802
Fragments arranged by size:
2041 1968 1802 507 205
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2114 2205
Size: 54 1425 50 275 18 189 95 91
Cuts at: 2205 3970 4859 6488 8
Size: 1765 889 1629 43
Fragments arranged by size:
1765 1629 1425 889 275 189 95 91
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 3970 1537
Size: 275 207 1951 4090
Fragments arranged by size:
4090 1951 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2189 2570 2792 2964 4453 1353
Size: 696 140 381 222 172 1489 3423
Fragments arranged by size:
3423 1489 696 381 222 172 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3190 3190
Size: 6523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2432 2642 2732 2773 2841 2894
Size: 393 671 129 210 90 41 68 53
Cuts at: 2894 2972 3238 3372 3528 3615 3651 3777 4586
Size: 78 266 134 156 87 36 126 809
Cuts at: 4586 5183 5970 6238 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 809 787 671 597 393 268 266
210 156 134 129 126 90 87 78
68 53 41 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2633 2573
Size: 60 6463
EcoO109I rG'GnC_Cy
Cuts at: 2059 2120 2121 2323 2626 2856 3428 3473 3482
Size: 61 1 202 303 230 572 45 9
Cuts at: 3482 3491 3535 3536 3635 3636 3653 3654 3671
Size: 9 44 1 99 1 17 1 17
Cuts at: 3671 3672 3680 3743 3770 3788 2059
Size: 1 8 63 27 18 4794
Fragments arranged by size:
4794 572 303 230 202 99 63 61
45 44 27 18 17 17 9 9
8 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2418 3832 4210 2418
Size: 1414 378 4731
Fragments arranged by size:
4731 1414 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3601 3601
Size: 6523
FseI GG_CCGG'CC
Cuts at: 2116 2116
Size: 6523
FspI TGC'GCA
Cuts at: 2801 5029 2801
Size: 2228 4295
HaeII r_GCGC'y
Cuts at: 1678 3253 5670 1678
Size: 1575 2417 2531
Fragments arranged by size:
2531 2417 1575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 2814 2846 2930
Size: 32 135 32 665 32 545 32 84
Cuts at: 2930 2962 3754 3786 5164 5196 5238 5270 1373
Size: 32 792 32 1378 32 42 32 2626
Fragments arranged by size:
2626 1378 792 665 545 135 84 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 3867 4113 669
Size: 988 281 610 1319 246 3079
Fragments arranged by size:
3079 1319 988 610 281 246
HindIII A'AGCT_T
Cuts at: 748 2294 748
Size: 1546 4977
HpaI GTT'AAC
Cuts at: 2548 4113 2548
Size: 1565 4958
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 4122 4122
Size: 6523
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5637 5821 1305
Size: 4332 184 2007
Fragments arranged by size:
4332 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4701
Fragments arranged by size:
4701 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2165 3177 3305 4481 4840 4999
Size: 648 843 156 1012 128 1176 359 159
Cuts at: 4999 518
Size: 2042
Fragments arranged by size:
2042 1176 1012 843 648 359 159 156
128
MspA1I CmG'CkG
Cuts at: 1606 2127 2136 2377 2452 2603 2719 2793 2929
Size: 521 9 241 75 151 116 74 136
Cuts at: 2929 2935 3730 3939 4616 5764 6009 1606
Size: 6 795 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 795 677 521 245 241 209
151 136 116 75 74 9 6
NaeI GCC'GGC
Cuts at: 2023 2114 2023
Size: 91 6432
NcoI C'CATG_G
Cuts at: 513 1065 3738 513
Size: 552 2673 3298
Fragments arranged by size:
3298 2673 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NgoMIV G'CCGG_C
Cuts at: 2021 2112 2021
Size: 91 6432
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NsiI A_TGCA'T
Cuts at: 2982 2982
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 2086 2896 2980 3127 3881 5426 1132
Size: 97 857 810 84 147 754 1545 2229
Fragments arranged by size:
2229 1545 857 810 754 147 97 84
PasI CC'CwG_GG
Cuts at: 1348 2851 3478 3487 3641 1348
Size: 1503 627 9 154 4230
Fragments arranged by size:
4230 1503 627 154 9
PciI A'CATG_T
Cuts at: 5422 5422
Size: 6523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2326 2515 2557 2917 1108
Size: 1218 189 42 360 4714
Fragments arranged by size:
4714 1218 360 189 42
PfoI T'CCnGG_A
Cuts at: 2955 3243 3388 2955
Size: 288 145 6090
Fragments arranged by size:
6090 288 145
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PmlI CAC'GTG
Cuts at: 3115 3115
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2336 2368 4592 4624 6105 6137 2336
Size: 32 2224 32 1481 32 2722
Fragments arranged by size:
2722 2224 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2626 3482 3491 3770 2059
Size: 567 856 9 279 4812
Fragments arranged by size:
4812 856 567 279 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6523
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PspOMI G'GGCC_C
Cuts at: 2120 3472 3535 3635 3653 3671 2120
Size: 1352 63 100 18 18 4972
Fragments arranged by size:
4972 1352 100 63 18 18
PspXI vC'TCGA_Gb
Cuts at: 3549 3549
Size: 6523
PstI C_TGCA'G
Cuts at: 830 2132 2403 2662 2934 3875 830
Size: 1302 271 259 272 941 3478
Fragments arranged by size:
3478 1302 941 272 271 259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2377 2452 2603 2929 2935 2377
Size: 75 151 326 6 5965
Fragments arranged by size:
5965 326 151 75 6
SacI G_AGCT'C
Cuts at: 3192 3192
Size: 6523
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SanDI GG'GwC_CC
Cuts at: 3482 3770 3482
Size: 288 6235
SapI GCTCTTCn'nnn_
Cuts at: 2570 2792 2570
Size: 222 6301
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 4771 6483 4771
Size: 1712 4811
SexAI A'CCwGG_T
Cuts at: 2541 2901 2541
Size: 360 6163
SfcI C'TryA_G
Cuts at: 826 1045 2128 2399 2658 2930 3871 5006 5687
Size: 219 1083 271 259 272 941 1135 681
Cuts at: 5687 5878 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1083 941 681 272 271 259
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3543 3643 3652 3679 3543
Size: 100 9 27 6387
Fragments arranged by size:
6387 100 27 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3660 3786 3850 1872
Size: 1788 126 64 4545
Fragments arranged by size:
4545 1788 126 64
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3128 3549 4627 5528 5790 820
Size: 197 926 1185 421 1078 901 262 1553
Fragments arranged by size:
1553 1185 1078 926 901 421 262 197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 2896 3127 3881 1132
Size: 1764 231 754 3774
Fragments arranged by size:
3774 1764 754 231
SspI AAT'ATT
Cuts at: 5 52 4447 5
Size: 47 4395 2081
Fragments arranged by size:
4395 2081 47
StuI AGG'CCT
Cuts at: 1517 3564 1517
Size: 2047 4476
StyI C'CwwG_G
Cuts at: 513 1065 3738 3962 513
Size: 552 2673 224 3074
Fragments arranged by size:
3074 2673 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 2648 3191 4550 4567 4720 4905
Size: 1255 537 531 543 1359 17 153 185
Cuts at: 4905 5350 325
Size: 445 1498
Fragments arranged by size:
1498 1359 1255 543 537 531 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4769 6481 96
Size: 274 80 33 51 1443 2792 1712 138
Fragments arranged by size:
2792 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6483 6515 6483
Size: 32 6491
Tth111I GACn'n_nGTC
Cuts at: 1316 2275 3715 1316
Size: 959 1440 4124
Fragments arranged by size:
4124 1440 959
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 3773 2605
Size: 1168 5355
XhoI C'TCGA_G
Cuts at: 1943 3549 1943
Size: 1606 4917
XmaI C'CCGG_G
Cuts at: 1870 3658 3784 3848 1870
Size: 1788 126 64 4545
Fragments arranged by size:
4545 1788 126 64
XmnI GAAnn'nnTTC
Cuts at: 3348 4652 3348
Size: 1304 5219
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4331 276
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoRV FspAI KasI NarI NruI
PacI PsrI RsrII SacII SfoI SrfI SwaI