Restriction map for
pFN21AE1094
(Circular) (Six-base) MAPSORT of: pFN21AE1094.seq Check: 8835 from: 1 to: 7438
pFN21AE1094 7438 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3645 4781 1937
Size: 1708 1136 4594
Fragments arranged by size:
4594 1708 1136
Acc65I G'GTAC_C
Cuts at: 4759 4759
Size: 7438
AclI AA'CG_TT
Cuts at: 1563 3957 5565 5938 1563
Size: 2394 1608 373 3063
Fragments arranged by size:
3063 2394 1608 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2104 2946 4346 4392 5501 6885 7153 1632
Size: 472 842 1400 46 1109 1384 268 1917
Fragments arranged by size:
1917 1400 1384 1109 842 472 268 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 4092 6337 7314 1203
Size: 2889 2245 977 1327
Fragments arranged by size:
2889 2245 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 6167 6167
Size: 7438
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7438
AlwNI CAG_nnn'CTG
Cuts at: 4115 5181 6753 4115
Size: 1066 1572 4800
Fragments arranged by size:
4800 1572 1066
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 2126 2147 3966 4055 4351 4747 4973 5125
Size: 633 21 1819 89 296 396 226 152
Cuts at: 5125 7234 1493
Size: 2109 1697
Fragments arranged by size:
2109 1819 1697 633 396 296 226 152
89 21
AseI AT'TA_AT
Cuts at: 160 1032 4482 5992 160
Size: 872 3450 1510 1606
Fragments arranged by size:
3450 1606 1510 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 4763 1870
Size: 73 2820 4545
Fragments arranged by size:
4545 2820 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4477 4510 4477
Size: 33 7405
BamHI G'GATC_C
Cuts at: 4768 5135 4768
Size: 367 7071
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4759 6214 618
Size: 325 217 627 2972 1455 1842
Fragments arranged by size:
2972 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4757 1391
Size: 3366 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6467
BbvCI CC'TCA_GC
Cuts at: 2223 4575 2223
Size: 2352 5086
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5629 5663 1903
Size: 34 3692 34 3678
Fragments arranged by size:
3692 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5330 6540 5330
Size: 1210 6228
BclI T'GATC_A
Cuts at: 1548 2571 2902 1548
Size: 1023 331 6084
Fragments arranged by size:
6084 1023 331
BfrBI ATG'CAT
Cuts at: 2957 3254 2957
Size: 297 7141
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4177 6049 136
Size: 107 122 71 631 605 2505 1872 1525
Fragments arranged by size:
2505 1872 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2662 7433 2662
Size: 4771 2667
BlpI GC'TnA_GC
Cuts at: 2274 4855 2274
Size: 2581 4857
Bme1580I G_kGCm'C
Cuts at: 1790 4247 5499 6655 1790
Size: 2457 1252 1156 2573
Fragments arranged by size:
2573 2457 1252 1156
BmgBI CAC'GTC
Cuts at: 1318 2099 1318
Size: 781 6657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3696 5169 6127
Size: 416 53 83 399 24 2279 1473 958
Cuts at: 6127 442
Size: 1753
Fragments arranged by size:
2279 1753 1473 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2756 1056
Size: 1700 5738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2391 4058 6098 1239
Size: 1152 1667 2040 2579
Fragments arranged by size:
2579 2040 1667 1152
Bpu10I CC'TnA_GC
Cuts at: 2223 4575 2223
Size: 2352 5086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 5563 6428 6726 2567
Size: 2996 865 298 3279
Fragments arranged by size:
3279 2996 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6101 882
Size: 617 4602 2219
Fragments arranged by size:
4602 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4095 5229 493
Size: 711 2891 1134 2702
Fragments arranged by size:
2891 2702 1134 711
BsaBI GATnn'nnATC
Cuts at: 3078 7432 3078
Size: 4354 3084
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4447 5245 5627 6262
Size: 53 83 186 936 2914 798 382 635
Cuts at: 6262 275
Size: 1451
Fragments arranged by size:
2914 1451 936 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5871 6269 6543 6690 7101 5871
Size: 398 274 147 411 6208
Fragments arranged by size:
6208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4526 4542 4556 4572 1178
Size: 30 165 30 3123 16 14 16 4044
Fragments arranged by size:
4044 3123 165 30 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4419 1186
Size: 3233 4205
BseYI C'CCAG_C
Cuts at: 6641 7137 6641
Size: 496 6942
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2121 3233 3480 3517 3709 1163
Size: 573 385 1112 247 37 192 4892
Fragments arranged by size:
4892 1112 573 385 247 192 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2778 3933 5499 5584 6655 2778
Size: 1155 1566 85 1071 3561
Fragments arranged by size:
3561 1566 1155 1071 85
BsmI GAATG_Cn'
Cuts at: 2957 4949 5042 2957
Size: 1992 93 5353
Fragments arranged by size:
5353 1992 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2778 3933 4247 4757 5499 5584 6655
Size: 399 988 1155 314 510 742 85 1071
Cuts at: 6655 1391
Size: 2174
Fragments arranged by size:
2174 1155 1071 988 742 510 399 314
85
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 2152 5327 7057 2152
Size: 3175 1730 2533
Fragments arranged by size:
3175 2533 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4793 844
Size: 3949 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5325 1394
Size: 429 3502 3507
Fragments arranged by size:
3507 3502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2211 2388 5933 6107 65
Size: 1183 963 177 3545 174 1396
Fragments arranged by size:
3545 1396 1183 963 177 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6082 6269 1700
Size: 110 4272 187 2869
Fragments arranged by size:
4272 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 3516 5498 6510 3516
Size: 1982 1012 4444
Fragments arranged by size:
4444 1982 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2387 794
Size: 1593 5845
BstBI TT'CG_AA
Cuts at: 6242 6242
Size: 7438
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7438
BstYI r'GATC_y
Cuts at: 1972 2662 4768 5135 5520 5537 6989 7075 7087
Size: 690 2106 367 385 17 1452 86 12
Cuts at: 7087 7433 1972
Size: 346 1977
Fragments arranged by size:
2106 1977 1452 690 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3646 3646
Size: 7438
BtgI C'CryG_G
Cuts at: 513 1065 2004 4214 513
Size: 552 939 2210 3737
Fragments arranged by size:
3737 2210 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6699
Fragments arranged by size:
6699 683 56
BtsI GCAGTG_nn'
Cuts at: 4954 5748 5768 4954
Size: 794 20 6624
Fragments arranged by size:
6624 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7403
DraI TTT'AAA
Cuts at: 2334 2586 2743 4630 4663 4742 2334
Size: 252 157 1887 33 79 5030
Fragments arranged by size:
5030 1887 252 157 79 33
DraIII CAC_nnn'GTG
Cuts at: 3434 3434
Size: 7438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6445 809
Size: 507 5129 1802
Fragments arranged by size:
5129 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3570 4885 5774
Size: 54 1425 50 275 18 1740 1315 889
Cuts at: 5774 7403 8
Size: 1629 43
Fragments arranged by size:
1740 1629 1425 1315 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 3276 5368 1353
Size: 813 1110 2092 3423
Fragments arranged by size:
3423 2092 1110 813
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6025 6399 6545 387
Size: 1064 138 4436 374 146 1280
Fragments arranged by size:
4436 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 2391 2946 4058 4346 4392 5501
Size: 393 472 287 555 1112 288 46 1109
Cuts at: 5501 6098 6885 7153 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1112 1109 787 597 555 472 393
288 287 268 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2700 2925 2700
Size: 225 7213
EcoRI G'AATT_C
Cuts at: 4747 5125 4747
Size: 378 7060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2951 2951
Size: 7438
FspI TGC'GCA
Cuts at: 5944 5944
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 6585 1678
Size: 4907 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3479 3511 4568 4600 6079
Size: 32 135 32 1907 32 1057 32 1479
Cuts at: 6079 6111 6153 6185 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1907 1479 1057 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3961 4782 5028 669
Size: 988 281 2023 821 246 3079
Fragments arranged by size:
3079 2023 988 821 281 246
HindIII A'AGCT_T
Cuts at: 748 2276 2588 4204 4632 748
Size: 1528 312 1616 428 3554
Fragments arranged by size:
3554 1616 1528 428 312
HpaI GTT'AAC
Cuts at: 3961 5028 3961
Size: 1067 6371
KpnI G_GTAC'C
Cuts at: 4763 4763
Size: 7438
MfeI C'AATT_G
Cuts at: 2466 5037 2466
Size: 2571 4867
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3385 6552 6736 1305
Size: 2080 3167 184 2007
Fragments arranged by size:
3167 2080 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3572 10
Size: 54 1425 343 1740 3876
Fragments arranged by size:
3876 1740 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2178 2364 2631 2853 4127 4614 5396
Size: 648 1012 186 267 222 1274 487 782
Cuts at: 5396 5755 5914 518
Size: 359 159 2042
Fragments arranged by size:
2042 1274 1012 782 648 487 359 267
222 186 159
MspA1I CmG'CkG
Cuts at: 1606 4854 5531 6679 6924 1606
Size: 3248 677 1148 245 2120
Fragments arranged by size:
3248 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4214 513
Size: 552 939 2210 3737
Fragments arranged by size:
3737 2210 939 552
NdeI CA'TA_TG
Cuts at: 387 4239 387
Size: 3852 3586
NheI G'CTAG_C
Cuts at: 1052 2752 1052
Size: 1700 5738
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NsiI A_TGCA'T
Cuts at: 2959 3256 2959
Size: 297 7141
NspI r_CATG'y
Cuts at: 1132 1229 4796 6341 1132
Size: 97 3567 1545 2229
Fragments arranged by size:
3567 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7438
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3579 1108
Size: 2471 4967
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 2528 5008 2528
Size: 2480 4958
PstI C_TGCA'G
Cuts at: 830 4790 830
Size: 3960 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SapI GCTCTTCn'nnn_
Cuts at: 2166 2166
Size: 7438
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SfcI C'TryA_G
Cuts at: 826 1045 2338 2658 2677 2700 4786 5921 6602
Size: 219 1293 320 19 23 2086 1135 681
Cuts at: 6602 6793 826
Size: 191 1471
Fragments arranged by size:
2086 1471 1293 1135 681 320 219 191
23 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 4765 1872
Size: 2893 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 5542 6443 6705 820
Size: 197 926 603 2996 901 262 1553
Fragments arranged by size:
2996 1553 926 901 603 262 197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7438
StyI C'CwwG_G
Cuts at: 513 1065 2004 4214 4530 4877 513
Size: 552 939 2210 316 347 3074
Fragments arranged by size:
3074 2210 939 552 347 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2362 5465 5482 5635 5820 6265 325
Size: 1255 782 3103 17 153 185 445 1498
Fragments arranged by size:
3103 1498 1255 782 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4072 5684 7396
Size: 274 80 33 51 1443 2095 1612 1712
Cuts at: 7396 96
Size: 138
Fragments arranged by size:
2095 1712 1612 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7398 7430 7398
Size: 32 7406
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7438
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 4763 1870
Size: 2893 4545
XmnI GAAnn'nnTTC
Cuts at: 5567 5567
Size: 7438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstXI Bsu36I EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI