Restriction map for
pFN21AE1091
(Circular) (Six-base) MAPSORT of: pFN21AE1091.seq Check: 3846 from: 1 to: 5689
pFN21AE1091 5689 bp
With 182 enzymes: SgfI *
October 14, 2009 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2366 2366
Size: 5689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2061 3032 1937
Size: 124 971 4594
Fragments arranged by size:
4594 971 124
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AfeI AGC'GCT
Cuts at: 2375 2375
Size: 5689
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2189 2415 1166
Size: 1023 226 4440
Fragments arranged by size:
4440 1023 226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2035 2482 2687 3432 5004 2035
Size: 447 205 745 1572 2720
Fragments arranged by size:
2720 1572 745 447 205
ApaI G_GGCC'C
Cuts at: 2176 2176
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2176 2949 3008 1391
Size: 785 773 59 4072
Fragments arranged by size:
4072 785 773 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BbvCI CC'TCA_GC
Cuts at: 2543 2543
Size: 5689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2784 2818 3880 3914 1903
Size: 34 847 34 1062 34 3678
Fragments arranged by size:
3678 1062 847 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 2272 1548
Size: 724 4965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2630 2673 2723
Size: 107 122 71 631 605 958 43 50
Cuts at: 2723 2792 4300 136
Size: 69 1508 1525
Fragments arranged by size:
1525 1508 958 631 605 122 107 71
69 50 43
BglII A'GATC_T
Cuts at: 2154 5684 2154
Size: 3530 2159
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2176 3750 4906 1790
Size: 386 1574 1156 2573
Fragments arranged by size:
2573 1574 1156 386
BmgBI CAC'GTC
Cuts at: 1318 2163 1318
Size: 845 4844
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 3420 4378
Size: 416 53 83 399 24 1053 950 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1053 958 950 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4349 1239
Size: 3110 2579
Bpu10I CC'TnA_GC
Cuts at: 2543 2543
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2807 2814 3496 3878
Size: 53 83 186 936 1274 7 682 382
Cuts at: 3878 4513 275
Size: 635 1451
Fragments arranged by size:
1451 1274 936 682 635 382 186 83
53 7
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2931 2961 1178
Size: 30 165 30 1528 30 3906
Fragments arranged by size:
3906 1528 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5689
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2143 2318 2806 1163
Size: 573 407 175 488 4046
Fragments arranged by size:
4046 573 488 407 175
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2190 2867 3139 3900 4928 1540
Size: 275 186 189 677 272 761 1028 2301
Fragments arranged by size:
2301 1028 761 677 275 272 189 186
BsiHKAI G_wGCw'C
Cuts at: 2949 3750 3835 4906 2949
Size: 801 85 1071 3732
Fragments arranged by size:
3732 1071 801 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2949 3008 3750 3835 4906 1391
Size: 399 386 773 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 773 742 399 386 85 59
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2340 2366 3044 844
Size: 1385 111 26 678 3489
Fragments arranged by size:
3489 1385 678 111 26
BsrBI CCG'CTC
Cuts at: 1394 1823 2701 3576 1394
Size: 429 878 875 3507
Fragments arranged by size:
3507 878 875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 4333 4520 1700
Size: 110 1121 1402 187 2869
Fragments arranged by size:
2869 1402 1121 187 110
BsrGI T'GTAC_A
Cuts at: 96 2193 96
Size: 2097 3592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2086 2532 794
Size: 1292 446 3951
Fragments arranged by size:
3951 1292 446
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2154 3019 3386 3771 3788 5240 5326 5338
Size: 182 865 367 385 17 1452 86 12
Cuts at: 5338 5684 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 865 385 367 346 182 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2727 513
Size: 552 939 723 3475
Fragments arranged by size:
3475 939 723 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2802 3205 3999 4019 2802
Size: 403 794 20 4472
Fragments arranged by size:
4472 794 403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2463 2559
Size: 54 1425 50 275 18 357 276 96
Cuts at: 2559 2724 2864 3136 4025 5654 8
Size: 165 140 272 889 1629 43
Fragments arranged by size:
1629 1425 889 357 276 275 272 165
140 96 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2187 2864 3136 1537
Size: 275 375 677 272 4090
Fragments arranged by size:
4090 677 375 275 272
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 2354 2909 3619 1353
Size: 971 30 555 710 3423
Fragments arranged by size:
3423 971 710 555 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2918 4276 4650 4796 387
Size: 1064 138 1329 1358 374 146 1280
Fragments arranged by size:
1358 1329 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2947 2947
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3752 4349 5136 5404 1239
Size: 393 2120 597 787 268 1524
Fragments arranged by size:
2120 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2173 2173
Size: 5689
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2377 4836 1678
Size: 699 2459 2531
Fragments arranged by size:
2531 2459 699
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2968 3000 4330 4362 4404
Size: 32 135 32 1396 32 1330 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1396 1330 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2561 10
Size: 54 1425 343 729 3138
Fragments arranged by size:
3138 1425 729 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2415 2726 3647 4006 4165 518
Size: 648 1023 226 311 921 359 159 2042
Fragments arranged by size:
2042 1023 921 648 359 311 226 159
MspA1I CmG'CkG
Cuts at: 1606 2131 2398 2846 3105 3782 4930 5175 1606
Size: 525 267 448 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 525 448 267 259 245
NaeI GCC'GGC
Cuts at: 2933 2933
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2931 2931
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2035 2398 1108
Size: 902 25 363 4399
Fragments arranged by size:
4399 902 363 25
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2172 2172
Size: 5689
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SacI G_AGCT'C
Cuts at: 2949 2949
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2951 3037 4172 4853 5044 826
Size: 219 1906 86 1135 681 191 1471
Fragments arranged by size:
1906 1471 1135 681 219 191 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 2723 2723
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 2931 1700
Size: 1231 4458
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2837 1517
Size: 1320 4369
StyI C'CwwG_G
Cuts at: 513 1065 2004 2266 3128 513
Size: 552 939 262 862 3074
Fragments arranged by size:
3074 939 862 552 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2062 2579 2930 3716 3733 3886 4071
Size: 1255 482 517 351 786 17 153 185
Cuts at: 4071 4516 325
Size: 445 1498
Fragments arranged by size:
1498 1255 786 517 482 445 351 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 3935 5647
Size: 274 80 33 51 1443 216 1742 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1742 1712 1443 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 5649 5681 2931
Size: 32 2686 32 2939
Fragments arranged by size:
2939 2686 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2702 2887 2702
Size: 185 5504
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 2924 3818 2924
Size: 894 4795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfoI SrfI SwaI