Restriction map for
pFN21AE1080
(Circular) (Six-base) MAPSORT of: pFN21AE1080.seq Check: 3061 from: 1 to: 8479
pFN21AE1080 8479 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2747 2747
Size: 8479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6289 278
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3858 5822 1937
Size: 1921 1964 4594
Fragments arranged by size:
4594 1964 1921
Acc65I G'GTAC_C
Cuts at: 5800 5800
Size: 8479
AclI AA'CG_TT
Cuts at: 1563 5647 6606 6979 1563
Size: 4084 959 373 3063
Fragments arranged by size:
4084 3063 959 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2059 2696 3261 4335 5322 5694 6542 7926
Size: 427 637 565 1074 987 372 848 1384
Cuts at: 7926 8194 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1074 987 848 637 565 427
372 268
AflII C'TTAA_G
Cuts at: 820 1017 5635 820
Size: 197 4618 3664
Fragments arranged by size:
4618 3664 197
AflIII A'CryG_T
Cuts at: 1203 3578 3968 4375 5053 7378 8355 1203
Size: 2375 390 407 678 2325 977 1327
Fragments arranged by size:
2375 2325 1327 977 678 407 390
AgeI A'CCGG_T
Cuts at: 7310 7310
Size: 8479
AhdI GACnn_n'nnGTC
Cuts at: 7208 7208
Size: 8479
AleI CACnn'nnGTG
Cuts at: 1166 3786 1166
Size: 2620 5859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 8447
AlwNI CAG_nnn'CTG
Cuts at: 2748 4991 5567 6222 7794 2748
Size: 2243 576 655 1572 3433
Fragments arranged by size:
3433 2243 1572 655 576
ApaLI G'TGCA_C
Cuts at: 5530 6536 7692 5530
Size: 1006 1156 6317
Fragments arranged by size:
6317 1156 1006
ApoI r'AATT_y
Cuts at: 1493 2348 2367 2918 3052 3121 3329 4444 4478
Size: 855 19 551 134 69 208 1115 34
Cuts at: 4478 5036 5185 5337 5472 5788 6014 6166 8275
Size: 558 149 152 135 316 226 152 2109
Cuts at: 8275 1493
Size: 1697
Fragments arranged by size:
2109 1697 1115 855 558 551 316 226
208 152 152 149 135 134 69 34
19
AseI AT'TA_AT
Cuts at: 160 1032 3251 7033 160
Size: 872 2219 3782 1606
Fragments arranged by size:
3782 2219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
AvaI C'yCGr_G
Cuts at: 1870 1943 3381 5804 1870
Size: 73 1438 2423 4545
Fragments arranged by size:
4545 2423 1438 73
AvrII C'CTAG_G
Cuts at: 2549 4214 2549
Size: 1665 6814
BamHI G'GATC_C
Cuts at: 5809 6176 5809
Size: 367 8112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3211 5800 7255 618
Size: 325 217 627 1424 2589 1455 1842
Fragments arranged by size:
2589 1842 1455 1424 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4390 5798 1391
Size: 2999 1408 4072
Fragments arranged by size:
4072 2999 1408
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2570 4113 4359 928
Size: 971 671 1543 246 5048
Fragments arranged by size:
5048 1543 971 671 246
BbvCI CC'TCA_GC
Cuts at: 2664 5752 2664
Size: 3088 5391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2606 2626 2640 2660 6670 6704 1903
Size: 34 669 20 14 20 4010 34 3678
Fragments arranged by size:
4010 3678 669 34 34 20 20 14
BciVI GTATCCnnnnn_n'
Cuts at: 3824 6371 7581 3824
Size: 2547 1210 4722
Fragments arranged by size:
4722 2547 1210
BclI T'GATC_A
Cuts at: 1548 3931 4936 1548
Size: 2383 1005 5091
Fragments arranged by size:
5091 2383 1005
BfrBI ATG'CAT
Cuts at: 2237 4781 4808 2237
Size: 2544 27 5908
Fragments arranged by size:
5908 2544 27
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7090 136
Size: 107 122 71 631 605 5418 1525
Fragments arranged by size:
5418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8474 8474
Size: 8479
BlpI GC'TnA_GC
Cuts at: 5896 5896
Size: 8479
Bme1580I G_kGCm'C
Cuts at: 1790 5534 5676 6540 7696 1790
Size: 3744 142 864 1156 2573
Fragments arranged by size:
3744 2573 1156 864 142
BmgBI CAC'GTC
Cuts at: 1318 2499 4357 1318
Size: 1181 1858 5440
Fragments arranged by size:
5440 1858 1181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4296 6210 7168
Size: 416 53 83 399 24 2879 1914 958
Cuts at: 7168 442
Size: 1753
Fragments arranged by size:
2879 1914 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2362 4820 7139 1239
Size: 1123 2458 2319 2579
Fragments arranged by size:
2579 2458 2319 1123
Bpu10I CC'TnA_GC
Cuts at: 2664 5752 2664
Size: 3088 5391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4403 6604 7469 7767 4403
Size: 2201 865 298 5115
Fragments arranged by size:
5115 2201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7142 882
Size: 617 5643 2219
Fragments arranged by size:
5643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3971 6270 493
Size: 711 2767 2299 2702
Fragments arranged by size:
2767 2702 2299 711
BsaBI GATnn'nnATC
Cuts at: 5145 8473 5145
Size: 3328 5151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4305 6286 6668 7303
Size: 53 83 186 936 2772 1981 382 635
Cuts at: 7303 275
Size: 1451
Fragments arranged by size:
2772 1981 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6912 7310 7584 7731 8142 6912
Size: 398 274 147 411 7249
Fragments arranged by size:
7249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3936 3966 5062 5092 1178
Size: 30 165 30 2533 30 1096 30 4565
Fragments arranged by size:
4565 2533 1096 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2681 4190 4193 4211 4214 5395 1186
Size: 1495 1509 3 18 3 1181 4270
Fragments arranged by size:
4270 1509 1495 1181 18 3 3
BseYI C'CCAG_C
Cuts at: 5766 7682 8178 5766
Size: 1916 496 6067
Fragments arranged by size:
6067 1916 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2610 1163
Size: 573 874 7032
Fragments arranged by size:
7032 874 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5929 6690 7718 1540
Size: 275 186 3928 761 1028 2301
Fragments arranged by size:
3928 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3992 4390 5534 6540 6625 7696 3992
Size: 398 1144 1006 85 1071 4775
Fragments arranged by size:
4775 1144 1071 1006 398 85
BsmI GAATG_Cn'
Cuts at: 3574 5693 5990 6083 3574
Size: 2119 297 93 5970
Fragments arranged by size:
5970 2119 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3992 4390 5534 5676 5798 6540 6625
Size: 399 2202 398 1144 142 122 742 85
Cuts at: 6625 7696 1391
Size: 1071 2174
Fragments arranged by size:
2202 2174 1144 1071 742 399 398 142
122 85
BspEI T'CCGG_A
Cuts at: 8142 8142
Size: 8479
BspHI T'CATG_A
Cuts at: 5361 6368 8098 5361
Size: 1007 1730 5742
Fragments arranged by size:
5742 1730 1007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 5834 844
Size: 1903 3087 3489
Fragments arranged by size:
3489 3087 1903
BsrBI CCG'CTC
Cuts at: 1394 1823 6366 1394
Size: 429 4543 3507
Fragments arranged by size:
4543 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2769 2892 3411 6974 7148 65
Size: 1183 1521 123 519 3563 174 1396
Fragments arranged by size:
3563 1521 1396 1183 519 174 123
BsrFI r'CCGG_y
Cuts at: 1700 1810 7123 7310 1700
Size: 110 5313 187 2869
Fragments arranged by size:
5313 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3625 96
Size: 3529 4950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8479
BssSI C'ACGA_G
Cuts at: 6539 7551 6539
Size: 1012 7467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8479
BstBI TT'CG_AA
Cuts at: 7283 7283
Size: 8479
BstEII G'GTnAC_C
Cuts at: 1184 2084 1184
Size: 900 7579
BstXI CCAn_nnnn'nTGG
Cuts at: 3674 3674
Size: 8479
BstYI r'GATC_y
Cuts at: 1972 3424 5131 5586 5809 6176 6561 6578 8030
Size: 1452 1707 455 223 367 385 17 1452
Cuts at: 8030 8116 8128 8474 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1707 1452 1452 455 385 367 346
223 86 17 12
BstZ17I GTA'TAC
Cuts at: 3859 3859
Size: 8479
Bsu36I CC'TnA_GG
Cuts at: 5591 5591
Size: 8479
BtgI C'CryG_G
Cuts at: 513 1065 2004 2131 2558 513
Size: 552 939 127 427 6434
Fragments arranged by size:
6434 939 552 427 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7740
Fragments arranged by size:
7740 683 56
BtsI GCAGTG_nn'
Cuts at: 5995 6789 6809 5995
Size: 794 20 7665
Fragments arranged by size:
7665 794 20
ClaI AT'CG_AT
Cuts at: 1554 2616 1554
Size: 1062 7417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8444
DraI TTT'AAA
Cuts at: 2242 5047 5701 5783 2242
Size: 2805 654 82 4938
Fragments arranged by size:
4938 2805 654 82
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7486 809
Size: 507 6170 1802
Fragments arranged by size:
6170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5926 6815 8444
Size: 54 1425 50 275 18 4096 889 1629
Cuts at: 8444 8
Size: 43
Fragments arranged by size:
4096 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5926 1537
Size: 275 4114 4090
Fragments arranged by size:
4114 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2672 2832 3201 3531 4167 4188 4311 4884
Size: 1319 160 369 330 636 21 123 573
Cuts at: 4884 5212 5609 5659 5713 5753 6409 1353
Size: 328 397 50 54 40 656 3423
Fragments arranged by size:
3423 1319 656 636 573 397 369 330
328 160 123 54 50 40 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4696 7066 7440 7586 387
Size: 1064 138 3107 2370 374 146 1280
Fragments arranged by size:
3107 2370 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4388 4388
Size: 8479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2362 2696 3261 4335 4820 5322
Size: 393 427 303 334 565 1074 485 502
Cuts at: 5322 5694 6542 7139 7926 8194 1239
Size: 372 848 597 787 268 1524
Fragments arranged by size:
1524 1074 848 787 597 565 502 485
427 393 372 334 303 268
EcoO109I rG'GnC_Cy
Cuts at: 2152 2606 3671 5763 2152
Size: 454 1065 2092 4868
Fragments arranged by size:
4868 2092 1065 454
EcoRI G'AATT_C
Cuts at: 2348 2367 4478 5788 6166 2348
Size: 19 2111 1310 378 4661
Fragments arranged by size:
4661 2111 1310 378 19
EcoRV GAT'ATC
Cuts at: 4651 4651
Size: 8479
FspI TGC'GCA
Cuts at: 6985 6985
Size: 8479
HaeII r_GCGC'y
Cuts at: 1678 7626 1678
Size: 5948 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3416 3448 3815 3847 3916
Size: 32 135 32 1844 32 367 32 69
Cuts at: 3916 3948 4295 4327 4621 4653 5062 5094 5246
Size: 32 347 32 294 32 409 32 152
Cuts at: 5246 5278 7120 7152 7194 7226 1373
Size: 32 1842 32 42 32 2626
Fragments arranged by size:
2626 1844 1842 409 367 347 294 152
135 69 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2261 3470 3829 3924 5298 5505
Size: 988 281 323 1209 359 95 1374 207
Cuts at: 5505 5823 6069 669
Size: 318 246 3079
Fragments arranged by size:
3079 1374 1209 988 359 323 318 281
246 207 95
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8479
HpaI GTT'AAC
Cuts at: 3829 5298 6069 3829
Size: 1469 771 6239
Fragments arranged by size:
6239 1469 771
KpnI G_GTAC'C
Cuts at: 5804 5804
Size: 8479
MfeI C'AATT_G
Cuts at: 4608 6078 4608
Size: 1470 7009
MluI A'CGCG_T
Cuts at: 8355 8355
Size: 8479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3010 3880 7593 7777 1305
Size: 1705 870 3713 184 2007
Fragments arranged by size:
3713 2007 1705 870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6657
Fragments arranged by size:
6657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3621 3786 6437 6796 6955 518
Size: 648 2455 165 2651 359 159 2042
Fragments arranged by size:
2651 2455 2042 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 3692 5564 5895 6572 7720 7965 1606
Size: 2086 1872 331 677 1148 245 2120
Fragments arranged by size:
2120 2086 1872 1148 677 331 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2131 513
Size: 552 939 127 6861
Fragments arranged by size:
6861 939 552 127
NdeI CA'TA_TG
Cuts at: 387 2233 387
Size: 1846 6633
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8479
NotI GC'GGCC_GC
Cuts at: 5926 5926
Size: 8479
NsiI A_TGCA'T
Cuts at: 2239 4783 4810 2239
Size: 2544 27 5908
Fragments arranged by size:
5908 2544 27
NspI r_CATG'y
Cuts at: 1132 1229 3582 3953 4379 5057 5837 7382 1132
Size: 97 2353 371 426 678 780 1545 2229
Fragments arranged by size:
2353 2229 1545 780 678 426 371 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8479
PciI A'CATG_T
Cuts at: 3578 4375 5053 7378 3578
Size: 797 678 2325 4679
Fragments arranged by size:
4679 2325 797 678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8479
PmeI GTTT'AAAC
Cuts at: 5783 5783
Size: 8479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4203 4235 6548 6580 8061 8093 4203
Size: 32 2313 32 1481 32 4589
Fragments arranged by size:
4589 2313 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3671 5763 3671
Size: 2092 6387
PshAI GACnn'nnGTC
Cuts at: 1613 3244 1613
Size: 1631 6848
PsiI TTA'TAA
Cuts at: 2378 5192 6049 2378
Size: 2814 857 4808
Fragments arranged by size:
4808 2814 857
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5414 5446 5414
Size: 32 8447
PstI C_TGCA'G
Cuts at: 830 2450 2744 3184 5831 830
Size: 1620 294 440 2647 3478
Fragments arranged by size:
3478 2647 1620 440 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8479
PvuII CAG'CTG
Cuts at: 3692 5564 3692
Size: 1872 6607
SacI G_AGCT'C
Cuts at: 4390 4390
Size: 8479
SalI G'TCGA_C
Cuts at: 1936 5821 1936
Size: 3885 4594
SapI GCTCTTCn'nnn_
Cuts at: 3531 5713 3531
Size: 2182 6297
SbfI CC_TGCA'GG
Cuts at: 5831 5831
Size: 8479
ScaI AGT'ACT
Cuts at: 6727 8439 6727
Size: 1712 6767
SfcI C'TryA_G
Cuts at: 826 1045 2254 2446 2724 2740 3031 3180 5827
Size: 219 1209 192 278 16 291 149 2647
Cuts at: 5827 6962 7643 7834 826
Size: 1135 681 191 1471
Fragments arranged by size:
2647 1471 1209 1135 681 291 278 219
192 191 149 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8479
SmaI CCC'GGG
Cuts at: 1872 5806 1872
Size: 3934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4418 5635 6583 7484 7746 820
Size: 197 926 2475 1217 948 901 262 1553
Fragments arranged by size:
2475 1553 1217 948 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6270 493
Size: 5777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8479
SphI G_CATG'C
Cuts at: 1132 5837 1132
Size: 4705 3774
SspI AAT'ATT
Cuts at: 5 52 3255 4905 4924 6403 5
Size: 47 3203 1650 19 1479 2081
Fragments arranged by size:
3203 2081 1650 1479 47 19
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8479
StyI C'CwwG_G
Cuts at: 513 1065 2004 2131 2549 3667 4214 5918 513
Size: 552 939 127 418 1118 547 1704 3074
Fragments arranged by size:
3074 1704 1118 939 552 547 418 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3289 6506 6523 6676 6861 7306 325
Size: 1255 1709 3217 17 153 185 445 1498
Fragments arranged by size:
3217 1709 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2733 3625 4082
Size: 274 80 33 51 1443 756 892 457
Cuts at: 4082 4135 5484 6725 8437 96
Size: 53 1349 1241 1712 138
Fragments arranged by size:
1712 1443 1349 1241 892 756 457 274
138 80 53 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8439 8471 8439
Size: 32 8447
Tth111I GACn'n_nGTC
Cuts at: 1316 4520 1316
Size: 3204 5275
XbaI T'CTAG_A
Cuts at: 4784 5815 4784
Size: 1031 7448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2503 2905 4631 5607 2503
Size: 402 1726 976 5375
Fragments arranged by size:
5375 1726 976 402
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8479
XmaI C'CCGG_G
Cuts at: 1870 5804 1870
Size: 3934 4545
XmnI GAAnn'nnTTC
Cuts at: 4158 4561 4619 6608 4158
Size: 403 58 1989 6029
Fragments arranged by size:
6029 1989 403 58
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6287 276
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BplI BsiWI BsmBI
DraIII EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI