Restriction map for
pFN21AE1074
(Circular) (Six-base) MAPSORT of: pFN21AE1074.seq Check: 811 from: 1 to: 6835
pFN21AE1074 6835 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4645 278
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3411 4178 1937
Size: 1474 767 4594
Fragments arranged by size:
4594 1474 767
Acc65I G'GTAC_C
Cuts at: 4156 4156
Size: 6835
AclI AA'CG_TT
Cuts at: 1563 4962 5335 1563
Size: 3399 373 3063
Fragments arranged by size:
3399 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4898 6282 6550 1632
Size: 3266 1384 268 1917
Fragments arranged by size:
3266 1917 1384 268
AfeI AGC'GCT
Cuts at: 2606 2606
Size: 6835
AflII C'TTAA_G
Cuts at: 820 1017 2978 820
Size: 197 1961 4677
Fragments arranged by size:
4677 1961 197
AflIII A'CryG_T
Cuts at: 1203 3045 5734 6711 1203
Size: 1842 2689 977 1327
Fragments arranged by size:
2689 1842 1327 977
AgeI A'CCGG_T
Cuts at: 5666 5666
Size: 6835
AhdI GACnn_n'nnGTC
Cuts at: 5564 5564
Size: 6835
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4008 1184
Size: 2824 4011
AlwNI CAG_nnn'CTG
Cuts at: 3562 4578 6150 3562
Size: 1016 1572 4247
Fragments arranged by size:
4247 1572 1016
ApaLI G'TGCA_C
Cuts at: 3791 4892 6048 3791
Size: 1101 1156 4578
Fragments arranged by size:
4578 1156 1101
ApoI r'AATT_y
Cuts at: 1493 4144 4370 4522 6631 1493
Size: 2651 226 152 2109 1697
Fragments arranged by size:
2651 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5389 160
Size: 872 4357 1606
Fragments arranged by size:
4357 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
AvaI C'yCGr_G
Cuts at: 1870 1943 4160 1870
Size: 73 2217 4545
Fragments arranged by size:
4545 2217 73
BamHI G'GATC_C
Cuts at: 3343 4165 4532 3343
Size: 822 367 5646
Fragments arranged by size:
5646 822 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4156 5611 618
Size: 325 217 627 2369 1455 1842
Fragments arranged by size:
2369 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 4154 1391
Size: 681 2082 4072
Fragments arranged by size:
4072 2082 681
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2702 2917 4079 928
Size: 971 803 215 1162 3684
Fragments arranged by size:
3684 1162 971 803 215
BbvCI CC'TCA_GC
Cuts at: 2768 2768
Size: 6835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5026 5060 1903
Size: 34 3089 34 3678
Fragments arranged by size:
3678 3089 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3628 3868 4727 5937 3628
Size: 240 859 1210 4526
Fragments arranged by size:
4526 1210 859 240
BclI T'GATC_A
Cuts at: 1548 2896 3150 1548
Size: 1348 254 5233
Fragments arranged by size:
5233 1348 254
BfrBI ATG'CAT
Cuts at: 2246 2519 2246
Size: 273 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5446 136
Size: 107 122 71 631 605 3774 1525
Fragments arranged by size:
3774 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3926 6830 3926
Size: 2904 3931
BlpI GC'TnA_GC
Cuts at: 3749 4252 3749
Size: 503 6332
Bme1580I G_kGCm'C
Cuts at: 1790 3795 4896 6052 1790
Size: 2005 1101 1156 2573
Fragments arranged by size:
2573 2005 1156 1101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6835
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4036 4566 5524
Size: 416 53 83 399 24 2619 530 958
Cuts at: 5524 442
Size: 1753
Fragments arranged by size:
2619 1753 958 530 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3011 3164 5495 1239
Size: 1772 153 2331 2579
Fragments arranged by size:
2579 2331 1772 153
Bpu10I CC'TnA_GC
Cuts at: 2768 2768
Size: 6835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3025 4960 5825 6123 3025
Size: 1935 865 298 3737
Fragments arranged by size:
3737 1935 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5498 882
Size: 617 3999 2219
Fragments arranged by size:
3999 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4626 493
Size: 711 3422 2702
Fragments arranged by size:
3422 2702 711
BsaBI GATnn'nnATC
Cuts at: 6829 6829
Size: 6835
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4642 5024 5659 275
Size: 53 83 186 936 3109 382 635 1451
Fragments arranged by size:
3109 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5268 5666 5940 6087 6498 5268
Size: 398 274 147 411 5605
Fragments arranged by size:
5605 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6610
Fragments arranged by size:
6610 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6835
BseYI C'CCAG_C
Cuts at: 6038 6534 6038
Size: 496 6339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 1163
Size: 573 1041 5221
Fragments arranged by size:
5221 1041 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4285 5046 6074 1540
Size: 275 186 2284 761 1028 2301
Fragments arranged by size:
2301 2284 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3460 3795 4896 4981 6052 3460
Size: 335 1101 85 1071 4243
Fragments arranged by size:
4243 1101 1071 335 85
BsmI GAATG_Cn'
Cuts at: 2211 2246 3104 3616 3997 4346 4439 2211
Size: 35 858 512 381 349 93 4607
Fragments arranged by size:
4607 858 512 381 349 93 35
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 3460 3795 4154 4896 4981 6052
Size: 399 282 1388 335 359 742 85 1071
Cuts at: 6052 1391
Size: 2174
Fragments arranged by size:
2174 1388 1071 742 399 359 335 282
85
BspEI T'CCGG_A
Cuts at: 6498 6498
Size: 6835
BspHI T'CATG_A
Cuts at: 2413 4724 6454 2413
Size: 2311 1730 2794
Fragments arranged by size:
2794 2311 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4190 844
Size: 3346 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4722 1394
Size: 429 2899 3507
Fragments arranged by size:
3507 2899 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 2847 4002 5330 5504 65
Size: 1183 1323 276 1155 1328 174 1396
Fragments arranged by size:
1396 1328 1323 1183 1155 276 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5479 5666 1700
Size: 110 3669 187 2869
Fragments arranged by size:
3669 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6835
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6835
BssSI C'ACGA_G
Cuts at: 4895 5907 4895
Size: 1012 5823
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2764 2972 794
Size: 1970 208 4657
Fragments arranged by size:
4657 1970 208
BstBI TT'CG_AA
Cuts at: 5639 5639
Size: 6835
BstEII G'GTnAC_C
Cuts at: 1184 2773 1184
Size: 1589 5246
BstYI r'GATC_y
Cuts at: 1972 3196 3343 3926 4165 4532 4917 4934 6386
Size: 1224 147 583 239 367 385 17 1452
Cuts at: 6386 6472 6484 6830 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1224 583 385 367 346 239
147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3127 3701 513
Size: 552 2062 574 3647
Fragments arranged by size:
3647 2062 574 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6096
Fragments arranged by size:
6096 683 56
BtsI GCAGTG_nn'
Cuts at: 4351 5145 5165 4351
Size: 794 20 6021
Fragments arranged by size:
6021 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6800
DraI TTT'AAA
Cuts at: 2187 2257 4139 2187
Size: 70 1882 4883
Fragments arranged by size:
4883 1882 70
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5842 809
Size: 507 4526 1802
Fragments arranged by size:
4526 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4282 5171 6800
Size: 54 1425 50 275 18 2452 889 1629
Cuts at: 6800 8
Size: 43
Fragments arranged by size:
2452 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4282 1537
Size: 275 2470 4090
Fragments arranged by size:
4090 2470 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3206 3323 3400 4104 4765 1353
Size: 1853 117 77 704 661 3423
Fragments arranged by size:
3423 1853 704 661 117 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5422 5796 5942 387
Size: 1064 138 3833 374 146 1280
Fragments arranged by size:
3833 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3011 3164 4898 5495 6282 6550 1239
Size: 393 1379 153 1734 597 787 268 1524
Fragments arranged by size:
1734 1524 1379 787 597 393 268 153
EcoRI G'AATT_C
Cuts at: 4144 4522 4144
Size: 378 6457
EcoRV GAT'ATC
Cuts at: 2221 2231 2221
Size: 10 6825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2423 2453 2423
Size: 30 6805
FspI TGC'GCA
Cuts at: 5341 5341
Size: 6835
HaeII r_GCGC'y
Cuts at: 1678 2534 2608 5982 1678
Size: 856 74 3374 2531
Fragments arranged by size:
3374 2531 856 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3335 3367 3454 3486 3698
Size: 32 135 32 1763 32 87 32 212
Cuts at: 3698 3730 5476 5508 5550 5582 1373
Size: 32 1746 32 42 32 2626
Fragments arranged by size:
2626 1763 1746 212 135 87 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2826 4179 4425 669
Size: 988 281 72 816 1353 246 3079
Fragments arranged by size:
3079 1353 988 816 281 246 72
HindIII A'AGCT_T
Cuts at: 748 2726 748
Size: 1978 4857
HpaI GTT'AAC
Cuts at: 4425 4425
Size: 6835
KpnI G_GTAC'C
Cuts at: 4160 4160
Size: 6835
MfeI C'AATT_G
Cuts at: 2574 4434 2574
Size: 1860 4975
MluI A'CGCG_T
Cuts at: 6711 6711
Size: 6835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5949 6133 1305
Size: 4644 184 2007
Fragments arranged by size:
4644 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5013
Fragments arranged by size:
5013 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 3726 3990 4793 5152 5311 518
Size: 648 1398 1162 264 803 359 159 2042
Fragments arranged by size:
2042 1398 1162 803 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 3697 3703 4251 4928 6076 6321 1606
Size: 2091 6 548 677 1148 245 2120
Fragments arranged by size:
2120 2091 1148 677 548 245 6
NcoI C'CATG_G
Cuts at: 513 1065 3127 513
Size: 552 2062 4221
Fragments arranged by size:
4221 2062 552
NdeI CA'TA_TG
Cuts at: 387 2661 387
Size: 2274 4561
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6835
NotI GC'GGCC_GC
Cuts at: 4282 4282
Size: 6835
NsiI A_TGCA'T
Cuts at: 2248 2521 2248
Size: 273 6562
NspI r_CATG'y
Cuts at: 1132 1229 3049 4193 5738 1132
Size: 97 1820 1144 1545 2229
Fragments arranged by size:
2229 1820 1545 1144 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6835
PciI A'CATG_T
Cuts at: 3045 5734 3045
Size: 2689 4146
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3193 1108
Size: 2085 4750
PfoI T'CCnGG_A
Cuts at: 3090 3090
Size: 6835
PmeI GTTT'AAAC
Cuts at: 4139 4139
Size: 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4904 4936 6417 6449 4904
Size: 32 1481 32 5290
Fragments arranged by size:
5290 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6835
PsiI TTA'TAA
Cuts at: 4405 4405
Size: 6835
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 6803
PstI C_TGCA'G
Cuts at: 830 4187 830
Size: 3357 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6835
PvuII CAG'CTG
Cuts at: 3697 3697
Size: 6835
SacII CC_GC'GG
Cuts at: 3704 3704
Size: 6835
SalI G'TCGA_C
Cuts at: 1936 4177 1936
Size: 2241 4594
SapI GCTCTTCn'nnn_
Cuts at: 4104 4104
Size: 6835
SbfI CC_TGCA'GG
Cuts at: 4187 4187
Size: 6835
ScaI AGT'ACT
Cuts at: 3057 5083 6795 3057
Size: 2026 1712 3097
Fragments arranged by size:
3097 2026 1712
SfcI C'TryA_G
Cuts at: 826 1045 3888 4183 5318 5999 6190 826
Size: 219 2843 295 1135 681 191 1471
Fragments arranged by size:
2843 1471 1135 681 295 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6835
SmaI CCC'GGG
Cuts at: 1872 4162 1872
Size: 2290 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2978 3004 4939 5840 6102 820
Size: 197 926 1035 26 1935 901 262 1553
Fragments arranged by size:
1935 1553 1035 926 901 262 197 26
SnaBI TAC'GTA
Cuts at: 493 4626 493
Size: 4133 2702
SpeI A'CTAG_T
Cuts at: 152 2890 152
Size: 2738 4097
SphI G_CATG'C
Cuts at: 1132 4193 1132
Size: 3061 3774
SspI AAT'ATT
Cuts at: 5 52 4759 5
Size: 47 4707 2081
Fragments arranged by size:
4707 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6835
StyI C'CwwG_G
Cuts at: 513 1065 2442 3127 3289 4274 513
Size: 552 1377 685 162 985 3074
Fragments arranged by size:
3074 1377 985 685 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3120 4862 4879 5032 5217 5662 325
Size: 1255 1540 1742 17 153 185 445 1498
Fragments arranged by size:
1742 1540 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2099 3055 5081
Size: 274 80 33 51 1443 122 956 2026
Cuts at: 5081 6793 96
Size: 1712 138
Fragments arranged by size:
2026 1712 1443 956 274 138 122 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6795 6827 6795
Size: 32 6803
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6835
XbaI T'CTAG_A
Cuts at: 4171 4171
Size: 6835
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6835
XmaI C'CCGG_G
Cuts at: 1870 4160 1870
Size: 2290 4545
XmnI GAAnn'nnTTC
Cuts at: 2035 2433 4098 4964 2035
Size: 398 1665 866 3906
Fragments arranged by size:
3906 1665 866 398
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4643 276
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI XcmI