Restriction map for
pFN21AE1073
(Circular) (Six-base) MAPSORT of: pFN21AE1073.seq Check: 4606 from: 1 to: 7138
pFN21AE1073 7138 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4948 278
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2070 2116 2325 4481 1937
Size: 133 46 209 2156 4594
Fragments arranged by size:
4594 2156 209 133 46
Acc65I G'GTAC_C
Cuts at: 4459 4459
Size: 7138
AclI AA'CG_TT
Cuts at: 1563 5265 5638 1563
Size: 3702 373 3063
Fragments arranged by size:
3702 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 2555 2586 2774 2871 4265 4340 5201
Size: 660 263 31 188 97 1394 75 861
Cuts at: 5201 6585 6853 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1394 1384 861 660 268 263 188
97 75 31
AflII C'TTAA_G
Cuts at: 820 1017 3779 820
Size: 197 2762 4179
Fragments arranged by size:
4179 2762 197
AflIII A'CryG_T
Cuts at: 1203 6037 7014 1203
Size: 4834 977 1327
Fragments arranged by size:
4834 1327 977
AgeI A'CCGG_T
Cuts at: 5969 5969
Size: 7138
AhdI GACnn_n'nnGTC
Cuts at: 5867 5867
Size: 7138
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2148 1184
Size: 964 6174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3054 3086 3054
Size: 32 7106
AlwNI CAG_nnn'CTG
Cuts at: 3824 4881 6453 3824
Size: 1057 1572 4509
Fragments arranged by size:
4509 1572 1057
ApaLI G'TGCA_C
Cuts at: 3417 5195 6351 3417
Size: 1778 1156 4204
Fragments arranged by size:
4204 1778 1156
ApoI r'AATT_y
Cuts at: 1493 2292 3702 4447 4673 4825 6934 1493
Size: 799 1410 745 226 152 2109 1697
Fragments arranged by size:
2109 1697 1410 799 745 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5692 160
Size: 872 4660 1606
Fragments arranged by size:
4660 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
AvaI C'yCGr_G
Cuts at: 1870 1943 3693 4463 1870
Size: 73 1750 770 4545
Fragments arranged by size:
4545 1750 770 73
BamHI G'GATC_C
Cuts at: 4121 4468 4835 4121
Size: 347 367 6424
Fragments arranged by size:
6424 367 347
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4459 5914 618
Size: 325 217 627 2672 1455 1842
Fragments arranged by size:
2672 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2085 3072 4299 4457 1391
Size: 694 987 1227 158 4072
Fragments arranged by size:
4072 1227 987 694 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5329 5363 1903
Size: 34 50 34 3308 34 3678
Fragments arranged by size:
3678 3308 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5030 6240 5030
Size: 1210 5928
BclI T'GATC_A
Cuts at: 1548 2185 1548
Size: 637 6501
BfrBI ATG'CAT
Cuts at: 3956 3956
Size: 7138
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5749 136
Size: 107 122 71 631 605 4077 1525
Fragments arranged by size:
4077 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7133 7133
Size: 7138
BlpI GC'TnA_GC
Cuts at: 3795 4555 3795
Size: 760 6378
Bme1580I G_kGCm'C
Cuts at: 1790 3421 3694 5199 6355 1790
Size: 1631 273 1505 1156 2573
Fragments arranged by size:
2573 1631 1505 1156 273
BmgBI CAC'GTC
Cuts at: 1318 2329 1318
Size: 1011 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4869 5827 442
Size: 416 53 83 399 24 3452 958 1753
Fragments arranged by size:
3452 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3491 1056
Size: 2435 4703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3056 4419 5798 1239
Size: 1817 1363 1379 2579
Fragments arranged by size:
2579 1817 1379 1363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3821 4127 5263 6128 6426 3821
Size: 306 1136 865 298 4533
Fragments arranged by size:
4533 1136 865 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5801 882
Size: 617 4302 2219
Fragments arranged by size:
4302 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4411 4929 493
Size: 711 3207 518 2702
Fragments arranged by size:
3207 2702 711 518
BsaBI GATnn'nnATC
Cuts at: 7132 7132
Size: 7138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4945 5327 5962 275
Size: 53 83 186 936 3412 382 635 1451
Fragments arranged by size:
3412 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2300 5571 5969 6243 6390 6801 2300
Size: 3271 398 274 147 411 2637
Fragments arranged by size:
3271 2637 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2313 2343 3054 3084 1178
Size: 30 165 30 910 30 711 30 5232
Fragments arranged by size:
5232 910 711 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3659 1186
Size: 2473 4665
BseYI C'CCAG_C
Cuts at: 6341 6837 6341
Size: 496 6642
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3154 4223 1163
Size: 573 1418 1069 4078
Fragments arranged by size:
4078 1418 1069 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2098 4588 5349 6377 1540
Size: 275 186 97 2490 761 1028 2301
Fragments arranged by size:
2490 2301 1028 761 275 186 97
BsiHKAI G_wGCw'C
Cuts at: 3072 3144 3421 4299 5199 5284 6355 3072
Size: 72 277 878 900 85 1071 3855
Fragments arranged by size:
3855 1071 900 878 277 85 72
BsmI GAATG_Cn'
Cuts at: 3200 3958 3974 4649 4742 3200
Size: 758 16 675 93 5596
Fragments arranged by size:
5596 758 675 93 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 3072 3144 3421 3694 4299 4457
Size: 399 295 987 72 277 273 605 158
Cuts at: 4457 5199 5284 6355 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 987 742 605 399 295 277
273 158 85 72
BspEI T'CCGG_A
Cuts at: 6801 6801
Size: 7138
BspHI T'CATG_A
Cuts at: 5027 6757 5027
Size: 1730 5408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4493 844
Size: 3649 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2100 2418 3745 3805 5025 1394
Size: 429 277 318 1327 60 1220 3507
Fragments arranged by size:
3507 1327 1220 429 318 277 60
BsrDI GCAATG_nn'
Cuts at: 65 1248 3756 5633 5807 65
Size: 1183 2508 1877 174 1396
Fragments arranged by size:
2508 1877 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5782 5969 1700
Size: 110 3972 187 2869
Fragments arranged by size:
3972 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7138
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7138
BssSI C'ACGA_G
Cuts at: 5198 6210 5198
Size: 1012 6126
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3734 794
Size: 2940 4198
BstBI TT'CG_AA
Cuts at: 3460 5942 3460
Size: 2482 4656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7138
BstYI r'GATC_y
Cuts at: 1972 4121 4468 4835 5220 5237 6689 6775 6787
Size: 2149 347 367 385 17 1452 86 12
Cuts at: 6787 7133 1972
Size: 346 1977
Fragments arranged by size:
2149 1977 1452 385 367 347 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2777 2777
Size: 7138
BtgI C'CryG_G
Cuts at: 513 1065 2356 513
Size: 552 1291 5295
Fragments arranged by size:
5295 1291 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6399
Fragments arranged by size:
6399 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4654 5448 5468 3334
Size: 1320 794 20 5004
Fragments arranged by size:
5004 1320 794 20
ClaI AT'CG_AT
Cuts at: 1554 3624 1554
Size: 2070 5068
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7103
DraI TTT'AAA
Cuts at: 3399 4161 4442 3399
Size: 762 281 6095
Fragments arranged by size:
6095 762 281
DraIII CAC_nnn'GTG
Cuts at: 2288 2288
Size: 7138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6145 809
Size: 507 4829 1802
Fragments arranged by size:
4829 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2095 4422
Size: 54 1425 50 275 18 189 76 2327
Cuts at: 4422 4585 5474 7103 8
Size: 163 889 1629 43
Fragments arranged by size:
2327 1629 1425 889 275 189 163 76
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2095 4585 1537
Size: 275 283 2490 4090
Fragments arranged by size:
4090 2490 283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3768 4173 5068 1353
Size: 2415 405 895 3423
Fragments arranged by size:
3423 2415 895 405
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3826 5725 6099 6245 387
Size: 1064 138 2237 1899 374 146 1280
Fragments arranged by size:
2237 1899 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3070 4297 3070
Size: 1227 5911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2555 2586 2774 2871 3056 4265
Size: 393 660 263 31 188 97 185 1209
Cuts at: 4265 4340 4419 5201 5798 6585 6853 1239
Size: 75 79 782 597 787 268 1524
Fragments arranged by size:
1524 1209 787 782 660 597 393 268
263 188 185 97 79 75 31
EcoRI G'AATT_C
Cuts at: 4447 4825 4447
Size: 378 6760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3410 3794 3410
Size: 384 6754
FspI TGC'GCA
Cuts at: 5644 5644
Size: 7138
HaeII r_GCGC'y
Cuts at: 1678 6285 1678
Size: 4607 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2245 2277 5779 5811 5853
Size: 32 135 32 673 32 3502 32 42
Cuts at: 5853 5885 1373
Size: 32 2626
Fragments arranged by size:
3502 2626 673 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2117 2455 3878 4482 4728 669
Size: 988 281 179 338 1423 604 246 3079
Fragments arranged by size:
3079 1423 988 604 338 281 246 179
HindIII A'AGCT_T
Cuts at: 748 3087 3251 748
Size: 2339 164 4635
Fragments arranged by size:
4635 2339 164
HpaI GTT'AAC
Cuts at: 4728 4728
Size: 7138
KpnI G_GTAC'C
Cuts at: 4463 4463
Size: 7138
MfeI C'AATT_G
Cuts at: 3605 4737 3605
Size: 1132 6006
MluI A'CGCG_T
Cuts at: 7014 7014
Size: 7138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2529 3022 6252 6436 1305
Size: 1224 493 3230 184 2007
Fragments arranged by size:
3230 2007 1224 493 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 4424 10
Size: 54 1425 343 189 2403 2724
Fragments arranged by size:
2724 2403 1425 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2751 5096 5455 5614 518
Size: 648 1585 2345 359 159 2042
Fragments arranged by size:
2345 2042 1585 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2575 4154 4554 5231 6379 6624 1606
Size: 506 463 1579 400 677 1148 245 2120
Fragments arranged by size:
2120 1579 1148 677 506 463 400 245
NcoI C'CATG_G
Cuts at: 513 1065 2356 513
Size: 552 1291 5295
Fragments arranged by size:
5295 1291 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7138
NheI G'CTAG_C
Cuts at: 1052 3487 1052
Size: 2435 4703
NotI GC'GGCC_GC
Cuts at: 4585 4585
Size: 7138
NsiI A_TGCA'T
Cuts at: 3958 3958
Size: 7138
NspI r_CATG'y
Cuts at: 1132 1229 2685 4496 6041 1132
Size: 97 1456 1811 1545 2229
Fragments arranged by size:
2229 1811 1545 1456 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7138
PciI A'CATG_T
Cuts at: 6037 6037
Size: 7138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7138
PfoI T'CCnGG_A
Cuts at: 3639 3639
Size: 7138
PmeI GTTT'AAAC
Cuts at: 4442 4442
Size: 7138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3054 3086 5207 5239 6720 6752 3054
Size: 32 2121 32 1481 32 3440
Fragments arranged by size:
3440 2121 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7138
PsiI TTA'TAA
Cuts at: 2243 4708 2243
Size: 2465 4673
PstI C_TGCA'G
Cuts at: 830 4490 830
Size: 3660 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7138
PvuII CAG'CTG
Cuts at: 4154 4154
Size: 7138
SacI G_AGCT'C
Cuts at: 3072 4299 3072
Size: 1227 5911
SalI G'TCGA_C
Cuts at: 1936 2115 4480 1936
Size: 179 2365 4594
Fragments arranged by size:
4594 2365 179
SapI GCTCTTCn'nnn_
Cuts at: 3768 3768
Size: 7138
SbfI CC_TGCA'GG
Cuts at: 4490 4490
Size: 7138
ScaI AGT'ACT
Cuts at: 3670 5386 7098 3670
Size: 1716 1712 3710
Fragments arranged by size:
3710 1716 1712
SfcI C'TryA_G
Cuts at: 826 1045 2519 3339 4486 5621 6302 6493 826
Size: 219 1474 820 1147 1135 681 191 1471
Fragments arranged by size:
1474 1471 1147 1135 820 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7138
SmaI CCC'GGG
Cuts at: 1872 4465 1872
Size: 2593 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3779 3800 4106 5242 6143 6405
Size: 197 926 1836 21 306 1136 901 262
Cuts at: 6405 820
Size: 1553
Fragments arranged by size:
1836 1553 1136 926 901 306 262 197
21
SnaBI TAC'GTA
Cuts at: 493 4929 493
Size: 4436 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7138
SphI G_CATG'C
Cuts at: 1132 2685 4496 1132
Size: 1553 1811 3774
Fragments arranged by size:
3774 1811 1553
SspI AAT'ATT
Cuts at: 5 52 5062 5
Size: 47 5010 2081
Fragments arranged by size:
5010 2081 47
StuI AGG'CCT
Cuts at: 1517 2528 1517
Size: 1011 6127
StyI C'CwwG_G
Cuts at: 513 1065 2016 2356 4577 513
Size: 552 951 340 2221 3074
Fragments arranged by size:
3074 2221 951 552 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2349 2606 5165 5182 5335 5520 5965
Size: 1255 769 257 2559 17 153 185 445
Cuts at: 5965 325
Size: 1498
Fragments arranged by size:
2559 1498 1255 769 445 257 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 3668 4231
Size: 274 80 33 51 1443 507 1184 563
Cuts at: 4231 5384 7096 96
Size: 1153 1712 138
Fragments arranged by size:
1712 1443 1184 1153 563 507 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2313 2345 7098 7130 2313
Size: 32 4753 32 2321
Fragments arranged by size:
4753 2321 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7138
XbaI T'CTAG_A
Cuts at: 4474 4474
Size: 7138
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7138
XmaI C'CCGG_G
Cuts at: 1870 4463 1870
Size: 2593 4545
XmnI GAAnn'nnTTC
Cuts at: 2818 5267 2818
Size: 2449 4689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4946 276
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI