Restriction map for
pFN21AE1072
(Circular) (Six-base) MAPSORT of: pFN21AE1072.seq Check: 4069 from: 1 to: 7018
pFN21AE1072 7018 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4828 278
Size: 53 83 186 936 3292 2468
Fragments arranged by size:
3292 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4361 1937
Size: 2424 4594
Acc65I G'GTAC_C
Cuts at: 4339 4339
Size: 7018
AclI AA'CG_TT
Cuts at: 1563 5145 5518 1563
Size: 3582 373 3063
Fragments arranged by size:
3582 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4197 5081 6465 6733 1632
Size: 2565 884 1384 268 1917
Fragments arranged by size:
2565 1917 1384 884 268
AfeI AGC'GCT
Cuts at: 3192 4051 3192
Size: 859 6159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6821
AflIII A'CryG_T
Cuts at: 1203 2850 3443 5917 6894 1203
Size: 1647 593 2474 977 1327
Fragments arranged by size:
2474 1647 1327 977 593
AgeI A'CCGG_T
Cuts at: 5849 5849
Size: 7018
AhdI GACnn_n'nnGTC
Cuts at: 2261 3044 5747 2261
Size: 783 2703 3532
Fragments arranged by size:
3532 2703 783
AleI CACnn'nnGTG
Cuts at: 1166 2168 3836 1166
Size: 1002 1668 4348
Fragments arranged by size:
4348 1668 1002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2800 1184
Size: 1616 5402
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2723 2755 3449 3481 2723
Size: 32 694 32 6260
Fragments arranged by size:
6260 694 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2258 2357 2508 4761 6333 2258
Size: 99 151 2253 1572 2943
Fragments arranged by size:
2943 2253 1572 151 99
ApaI G_GGCC'C
Cuts at: 4173 4173
Size: 7018
ApaLI G'TGCA_C
Cuts at: 5075 6231 5075
Size: 1156 5862
ApoI r'AATT_y
Cuts at: 1493 2390 2629 3008 4327 4553 4705 6814 1493
Size: 897 239 379 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 897 379 239 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5572 160
Size: 872 4540 1606
Fragments arranged by size:
4540 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7018
AvaI C'yCGr_G
Cuts at: 1870 1943 4053 4343 1870
Size: 73 2110 290 4545
Fragments arranged by size:
4545 2110 290 73
BamHI G'GATC_C
Cuts at: 2079 3969 4348 4715 2079
Size: 1890 379 367 4382
Fragments arranged by size:
4382 1890 379 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 3539 4339 5794 618
Size: 325 217 627 1148 604 800 1455 1842
Fragments arranged by size:
1842 1455 1148 800 627 604 325 217
BanII G_rGCy'C
Cuts at: 1391 3111 4173 4337 1391
Size: 1720 1062 164 4072
Fragments arranged by size:
4072 1720 1062 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4185 928
Size: 971 2286 3761
Fragments arranged by size:
3761 2286 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5209 5243 1903
Size: 34 3272 34 3678
Fragments arranged by size:
3678 3272 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4910 6120 4910
Size: 1210 5808
BclI T'GATC_A
Cuts at: 1548 3825 1548
Size: 2277 4741
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3657 5629 136
Size: 107 122 71 631 605 1985 1972 1525
Fragments arranged by size:
1985 1972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7013 7013
Size: 7018
BlpI GC'TnA_GC
Cuts at: 4435 4435
Size: 7018
Bme1580I G_kGCm'C
Cuts at: 1790 2210 3693 4173 5079 6235 1790
Size: 420 1483 480 906 1156 2573
Fragments arranged by size:
2573 1483 1156 906 480 420
BmgBI CAC'GTC
Cuts at: 1318 2853 3133 4096 1318
Size: 1535 280 963 4240
Fragments arranged by size:
4240 1535 963 280
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 2734 4171
Size: 416 53 83 399 24 713 604 1437
Cuts at: 4171 4749 5707 442
Size: 578 958 1753
Fragments arranged by size:
1753 1437 958 713 604 578 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2153 1056
Size: 1097 5921
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 7018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2447 2847 4296 5678 1239
Size: 820 388 400 1449 1382 2579
Fragments arranged by size:
2579 1449 1382 820 400 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3235 5143 6008 6306 3235
Size: 1908 865 298 3947
Fragments arranged by size:
3947 1908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2230 3213 3274 5681 882
Size: 617 731 983 61 2407 2219
Fragments arranged by size:
2407 2219 983 731 617 61
BsaAI yAC'GTr
Cuts at: 493 1204 4080 4809 493
Size: 711 2876 729 2702
Fragments arranged by size:
2876 2702 729 711
BsaBI GATnn'nnATC
Cuts at: 3239 7012 3239
Size: 3773 3245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2310 4825 5207 5842
Size: 53 83 186 936 777 2515 382 635
Cuts at: 5842 275
Size: 1451
Fragments arranged by size:
2515 1451 936 777 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3972 5451 5849 6123 6270 6681 3972
Size: 1479 398 274 147 411 4309
Fragments arranged by size:
4309 1479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2322 2352 2723 2753 3122
Size: 30 165 30 919 30 371 30 369
Cuts at: 3122 3152 1178
Size: 30 5044
Fragments arranged by size:
5044 919 371 369 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3185 3356 4133 1186
Size: 1999 171 777 4071
Fragments arranged by size:
4071 1999 777 171
BseYI C'CCAG_C
Cuts at: 3055 3430 3646 6221 6717 3055
Size: 375 216 2575 496 3356
Fragments arranged by size:
3356 2575 496 375 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 4052 1163
Size: 573 327 1989 4129
Fragments arranged by size:
4129 1989 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2451 4228 4468 5229 6257 1540
Size: 275 186 450 1777 240 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 450 275 240 186
BsiHKAI G_wGCw'C
Cuts at: 2464 3348 3714 5079 5164 6235 2464
Size: 884 366 1365 85 1071 3247
Fragments arranged by size:
3247 1365 1071 884 366 85
BsmI GAATG_Cn'
Cuts at: 3366 4529 4622 3366
Size: 1163 93 5762
Fragments arranged by size:
5762 1163 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2464 3111 3348 3693 3714 4173
Size: 399 420 254 647 237 345 21 459
Cuts at: 4173 4337 5079 5164 6235 1391
Size: 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 647 459 420 399 345
254 237 164 85 21
BspEI T'CCGG_A
Cuts at: 6681 6681
Size: 7018
BspHI T'CATG_A
Cuts at: 2688 4907 6637 2688
Size: 2219 1730 3069
Fragments arranged by size:
3069 2219 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4373 844
Size: 3529 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4905 1394
Size: 429 3082 3507
Fragments arranged by size:
3507 3082 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2073 2280 5513 5687 65
Size: 1183 825 207 3233 174 1396
Fragments arranged by size:
3233 1396 1183 825 207 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3669 5662 5849 1700
Size: 110 1859 1993 187 2869
Fragments arranged by size:
2869 1993 1859 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7018
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7018
BssSI C'ACGA_G
Cuts at: 5078 6090 5078
Size: 1012 6006
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7018
BstBI TT'CG_AA
Cuts at: 5822 5822
Size: 7018
BstEII G'GTnAC_C
Cuts at: 1184 3697 1184
Size: 2513 4505
BstXI CCAn_nnnn'nTGG
Cuts at: 2098 2248 3802 2098
Size: 150 1554 5314
Fragments arranged by size:
5314 1554 150
BstYI r'GATC_y
Cuts at: 1972 2079 2354 3969 4348 4715 5100 5117 6569
Size: 107 275 1615 379 367 385 17 1452
Cuts at: 6569 6655 6667 7013 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1615 1452 385 379 367 346 275
107 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2130 2749 3093 3543 513
Size: 552 1065 619 344 450 3988
Fragments arranged by size:
3988 1065 619 552 450 344
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2800 487
Size: 683 56 1574 4705
Fragments arranged by size:
4705 1574 683 56
BtsI GCAGTG_nn'
Cuts at: 2051 2442 2871 4534 5328 5348 2051
Size: 391 429 1663 794 20 3721
Fragments arranged by size:
3721 1663 794 429 391 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7018
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3427 3462 529
Size: 35 2863 35 4085
Fragments arranged by size:
4085 2863 35 35
DraI TTT'AAA
Cuts at: 4322 4322
Size: 7018
DraIII CAC_nnn'GTG
Cuts at: 3697 3697
Size: 7018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6025 809
Size: 507 4709 1802
Fragments arranged by size:
4709 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2448 3018
Size: 54 1425 50 275 18 408 210 570
Cuts at: 3018 4225 4465 5354 6983 8
Size: 1207 240 889 1629 43
Fragments arranged by size:
1629 1425 1207 889 570 408 275 240
210 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2448 4225 4465 1537
Size: 275 636 1777 240 4090
Fragments arranged by size:
4090 1777 636 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 2708 3071 3725 4053 4241 4948 1353
Size: 1355 363 654 328 188 707 3423
Fragments arranged by size:
3423 1355 707 654 363 328 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 2744 5605 5979 6125 387
Size: 1064 138 942 213 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 942 374 213 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2447 2847 4197 4296 5081 5678
Size: 393 427 388 400 1350 99 785 597
Cuts at: 5678 6465 6733 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1350 787 785 597 427 400 393
388 268 99
EcoO109I rG'GnC_Cy
Cuts at: 2255 3060 4063 4279 2255
Size: 805 1003 216 4994
Fragments arranged by size:
4994 1003 805 216
EcoRI G'AATT_C
Cuts at: 2629 4327 4705 2629
Size: 1698 378 4942
Fragments arranged by size:
4942 1698 378
FspI TGC'GCA
Cuts at: 5524 5524
Size: 7018
HaeII r_GCGC'y
Cuts at: 1678 3194 4053 6165 1678
Size: 1516 859 2112 2531
Fragments arranged by size:
2531 2112 1516 859
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2247 2279 2322
Size: 32 135 32 593 32 50 32 43
Cuts at: 2322 2354 2911 2943 2945 2977 3030 3062 3221
Size: 32 557 32 2 32 53 32 159
Cuts at: 3221 3253 3604 3636 3758 3790 4008 4040 4094
Size: 32 351 32 122 32 218 32 54
Cuts at: 4094 4126 5659 5691 5733 5765 1373
Size: 32 1533 32 42 32 2626
Fragments arranged by size:
2626 1533 593 557 351 218 159 135
122 54 53 50 43 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3510 3523 4362 4608 669
Size: 988 281 1572 13 839 246 3079
Fragments arranged by size:
3079 1572 988 839 281 246 13
HindIII A'AGCT_T
Cuts at: 748 3390 748
Size: 2642 4376
HpaI GTT'AAC
Cuts at: 4608 4608
Size: 7018
KpnI G_GTAC'C
Cuts at: 4343 4343
Size: 7018
MfeI C'AATT_G
Cuts at: 4617 4617
Size: 7018
MluI A'CGCG_T
Cuts at: 6894 6894
Size: 7018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6132 6316 1305
Size: 4827 184 2007
Fragments arranged by size:
4827 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 3020 10
Size: 54 1425 343 408 780 4008
Fragments arranged by size:
4008 1425 780 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2213 2219 2975 3707 3728 3836
Size: 648 1002 45 6 756 732 21 108
Cuts at: 3836 3926 4976 5335 5494 518
Size: 90 1050 359 159 2042
Fragments arranged by size:
2042 1050 1002 756 732 648 359 159
108 90 45 21 6
MspA1I CmG'CkG
Cuts at: 1606 2199 2508 4434 5111 6259 6504 1606
Size: 593 309 1926 677 1148 245 2120
Fragments arranged by size:
2120 1926 1148 677 593 309 245
NcoI C'CATG_G
Cuts at: 513 1065 3093 513
Size: 552 2028 4438
Fragments arranged by size:
4438 2028 552
NdeI CA'TA_TG
Cuts at: 387 2902 387
Size: 2515 4503
NheI G'CTAG_C
Cuts at: 1052 2149 1052
Size: 1097 5921
NotI GC'GGCC_GC
Cuts at: 4465 4465
Size: 7018
NspI r_CATG'y
Cuts at: 1132 1229 2218 2476 2787 3447 4376 5921 1132
Size: 97 989 258 311 660 929 1545 2229
Fragments arranged by size:
2229 1545 989 929 660 311 258 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7018
PciI A'CATG_T
Cuts at: 3443 5917 3443
Size: 2474 4544
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3966 1108
Size: 2858 4160
PfoI T'CCnGG_A
Cuts at: 2075 2424 2075
Size: 349 6669
PmeI GTTT'AAAC
Cuts at: 4322 4322
Size: 7018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2723 2755 5087 5119 6600 6632 2723
Size: 32 2332 32 1481 32 3109
Fragments arranged by size:
3109 2332 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2255 3060 2255
Size: 805 6213
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7018
PsiI TTA'TAA
Cuts at: 4588 4588
Size: 7018
PspOMI G'GGCC_C
Cuts at: 4169 4169
Size: 7018
PstI C_TGCA'G
Cuts at: 830 3855 4370 830
Size: 3025 515 3478
Fragments arranged by size:
3478 3025 515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7018
PvuII CAG'CTG
Cuts at: 2199 2199
Size: 7018
SalI G'TCGA_C
Cuts at: 1936 4360 1936
Size: 2424 4594
SanDI GG'GwC_CC
Cuts at: 3060 3060
Size: 7018
SapI GCTCTTCn'nnn_
Cuts at: 3725 4241 3725
Size: 516 6502
SbfI CC_TGCA'GG
Cuts at: 4370 4370
Size: 7018
ScaI AGT'ACT
Cuts at: 5266 6978 5266
Size: 1712 5306
SexAI A'CCwGG_T
Cuts at: 2257 2257
Size: 7018
SfcI C'TryA_G
Cuts at: 826 1045 2368 3851 4366 5501 6182 6373 826
Size: 219 1323 1483 515 1135 681 191 1471
Fragments arranged by size:
1483 1471 1323 1135 681 515 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7018
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7018
SmaI CCC'GGG
Cuts at: 1872 4345 1872
Size: 2473 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3214 4053 5122 6023 6285 820
Size: 197 926 1271 839 1069 901 262 1553
Fragments arranged by size:
1553 1271 1069 926 901 839 262 197
SnaBI TAC'GTA
Cuts at: 493 4809 493
Size: 4316 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7018
SphI G_CATG'C
Cuts at: 1132 4376 1132
Size: 3244 3774
SspI AAT'ATT
Cuts at: 5 52 3586 4942 5
Size: 47 3534 1356 2081
Fragments arranged by size:
3534 2081 1356 47
StuI AGG'CCT
Cuts at: 1517 2700 1517
Size: 1183 5835
StyI C'CwwG_G
Cuts at: 513 1065 2241 3093 3264 3468 4457 513
Size: 552 1176 852 171 204 989 3074
Fragments arranged by size:
3074 1176 989 852 552 204 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2668 5045 5062 5215 5400 5845 325
Size: 1255 1088 2377 17 153 185 445 1498
Fragments arranged by size:
2377 1498 1255 1088 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5264 6976 96
Size: 274 80 33 51 1443 3287 1712 138
Fragments arranged by size:
3287 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3122 3154 6978 7010 3122
Size: 32 3824 32 3130
Fragments arranged by size:
3824 3130 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7018
XbaI T'CTAG_A
Cuts at: 4354 4354
Size: 7018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3013 3013
Size: 7018
XhoI C'TCGA_G
Cuts at: 1943 4053 1943
Size: 2110 4908
XmaI C'CCGG_G
Cuts at: 1870 4343 1870
Size: 2473 4545
XmnI GAAnn'nnTTC
Cuts at: 5147 5147
Size: 7018
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4826 276
Size: 53 83 186 936 3292 2468
Fragments arranged by size:
3292 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SfiI
SfoI SrfI SwaI