Restriction map for
pFN21AE1069
(Circular) (Six-base) MAPSORT of: pFN21AE1069.seq Check: 2037 from: 1 to: 5236
pFN21AE1069 5236 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3046 278
Size: 53 83 186 936 1510 2468
Fragments arranged by size:
2468 1510 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2579 1937
Size: 642 4594
Acc65I G'GTAC_C
Cuts at: 2557 2557
Size: 5236
AclI AA'CG_TT
Cuts at: 1563 3363 3736 1563
Size: 1800 373 3063
Fragments arranged by size:
3063 1800 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2019 3299 4683 4951 1632
Size: 387 1280 1384 268 1917
Fragments arranged by size:
1917 1384 1280 387 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5039
AflIII A'CryG_T
Cuts at: 1203 4135 5112 1203
Size: 2932 977 1327
Fragments arranged by size:
2932 1327 977
AgeI A'CCGG_T
Cuts at: 4067 4067
Size: 5236
AhdI GACnn_n'nnGTC
Cuts at: 3965 3965
Size: 5236
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5236
AlwNI CAG_nnn'CTG
Cuts at: 2979 4551 2979
Size: 1572 3664
ApaLI G'TGCA_C
Cuts at: 3293 4449 3293
Size: 1156 4080
ApoI r'AATT_y
Cuts at: 1493 2545 2771 2923 5032 1493
Size: 1052 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3790 160
Size: 872 2758 1606
Fragments arranged by size:
2758 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5236
AvaI C'yCGr_G
Cuts at: 1870 1943 2146 2241 2561 1870
Size: 73 203 95 320 4545
Fragments arranged by size:
4545 320 203 95 73
BamHI G'GATC_C
Cuts at: 2566 2933 2566
Size: 367 4869
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2208 2426 2557 4012 618
Size: 325 217 627 421 218 131 1455 1842
Fragments arranged by size:
1842 1455 627 421 325 218 217 131
BanII G_rGCy'C
Cuts at: 1391 2555 1391
Size: 1164 4072
BbeI G_GCGC'C
Cuts at: 2430 2430
Size: 5236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3427 3461 1903
Size: 34 1490 34 3678
Fragments arranged by size:
3678 1490 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3128 4338 3128
Size: 1210 4026
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3847 136
Size: 107 122 71 631 605 2175 1525
Fragments arranged by size:
2175 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5231 5231
Size: 5236
BlpI GC'TnA_GC
Cuts at: 2459 2653 2459
Size: 194 5042
Bme1580I G_kGCm'C
Cuts at: 1790 2211 3297 4453 1790
Size: 421 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 421
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5236
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 3925 442
Size: 416 53 83 399 24 1550 958 1753
Fragments arranged by size:
1753 1550 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2333 2068
Size: 265 4971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2234 3896 1239
Size: 944 51 1662 2579
Fragments arranged by size:
2579 1662 944 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2236 3361 4226 4524 2236
Size: 1125 865 298 2948
Fragments arranged by size:
2948 1125 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3899 882
Size: 617 2400 2219
Fragments arranged by size:
2400 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3027 493
Size: 711 1823 2702
Fragments arranged by size:
2702 1823 711
BsaBI GATnn'nnATC
Cuts at: 5230 5230
Size: 5236
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2261 2427 3043 3425
Size: 53 83 186 936 728 166 616 382
Cuts at: 3425 4060 275
Size: 635 1451
Fragments arranged by size:
1451 936 728 635 616 382 186 166
83 53
BsaWI w'CCGG_w
Cuts at: 3669 4067 4341 4488 4899 3669
Size: 398 274 147 411 4006
Fragments arranged by size:
4006 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5011
Fragments arranged by size:
5011 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 1186
Size: 895 4341
BseYI C'CCAG_C
Cuts at: 2437 4439 4935 2437
Size: 2002 496 2738
Fragments arranged by size:
2738 2002 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 1163
Size: 573 303 4360
Fragments arranged by size:
4360 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2686 3447 4475 1540
Size: 275 186 685 761 1028 2301
Fragments arranged by size:
2301 1028 761 685 275 186
BsiHKAI G_wGCw'C
Cuts at: 3297 3382 4453 3297
Size: 85 1071 4080
Fragments arranged by size:
4080 1071 85
BsmI GAATG_Cn'
Cuts at: 2060 2747 2840 2060
Size: 687 93 4456
Fragments arranged by size:
4456 687 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2555 3297 3382 4453 1391
Size: 399 421 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 344 85
BspEI T'CCGG_A
Cuts at: 4899 4899
Size: 5236
BspHI T'CATG_A
Cuts at: 3125 4855 3125
Size: 1730 3506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2591 844
Size: 1235 512 3489
Fragments arranged by size:
3489 1235 512
BsrBI CCG'CTC
Cuts at: 1394 1823 3123 1394
Size: 429 1300 3507
Fragments arranged by size:
3507 1300 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3731 3905 65
Size: 1183 2483 174 1396
Fragments arranged by size:
2483 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3880 4067 1700
Size: 110 2070 187 2869
Fragments arranged by size:
2869 2070 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5236
BssSI C'ACGA_G
Cuts at: 3296 4308 3296
Size: 1012 4224
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5236
BstBI TT'CG_AA
Cuts at: 4040 4040
Size: 5236
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5236
BstYI r'GATC_y
Cuts at: 1972 2158 2566 2933 3318 3335 4787 4873 4885
Size: 186 408 367 385 17 1452 86 12
Cuts at: 4885 5231 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 408 385 367 346 186 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3745
Fragments arranged by size:
3745 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2254 487
Size: 683 56 1028 3469
Fragments arranged by size:
3469 1028 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 2752 3546 3566 2222
Size: 530 794 20 3892
Fragments arranged by size:
3892 794 530 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5201
DraI TTT'AAA
Cuts at: 2540 2540
Size: 5236
DraIII CAC_nnn'GTG
Cuts at: 2017 2017
Size: 5236
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4243 809
Size: 507 2927 1802
Fragments arranged by size:
2927 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2502 2683
Size: 54 1425 50 275 18 495 177 181
Cuts at: 2683 3572 5201 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 495 275 181 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2683 1537
Size: 275 871 4090
Fragments arranged by size:
4090 871 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3166 1353
Size: 1813 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 3823 4197 4343 387
Size: 1064 138 455 1779 374 146 1280
Fragments arranged by size:
1779 1280 1064 455 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 2183 2234 3299 3896 4683 4951
Size: 393 387 164 51 1065 597 787 268
Cuts at: 4951 1239
Size: 1524
Fragments arranged by size:
1524 1065 787 597 393 387 268 164
51
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 5236
EcoO109I rG'GnC_Cy
Cuts at: 2150 2179 2218 2150
Size: 29 39 5168
Fragments arranged by size:
5168 39 29
EcoRI G'AATT_C
Cuts at: 2545 2923 2545
Size: 378 4858
FspI TGC'GCA
Cuts at: 3742 3742
Size: 5236
HaeII r_GCGC'y
Cuts at: 1678 2430 4383 1678
Size: 752 1953 2531
Fragments arranged by size:
2531 1953 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 3877 3909 3951
Size: 32 135 32 541 32 1732 32 42
Cuts at: 3951 3983 1373
Size: 32 2626
Fragments arranged by size:
2626 1732 541 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2515 2580 2826 669
Size: 988 281 577 65 246 3079
Fragments arranged by size:
3079 988 577 281 246 65
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5236
HpaI GTT'AAC
Cuts at: 2826 2826
Size: 5236
KasI G'GCGC_C
Cuts at: 2426 2426
Size: 5236
KpnI G_GTAC'C
Cuts at: 2561 2561
Size: 5236
MfeI C'AATT_G
Cuts at: 2835 2835
Size: 5236
MluI A'CGCG_T
Cuts at: 5112 5112
Size: 5236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4350 4534 1305
Size: 3045 184 2007
Fragments arranged by size:
3045 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3414
Fragments arranged by size:
3414 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 2495 3194 3553 3712 518
Size: 648 1311 18 699 359 159 2042
Fragments arranged by size:
2042 1311 699 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2652 3329 4477 4722 1606
Size: 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 245
NarI GG'CG_CC
Cuts at: 2427 2427
Size: 5236
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3745
Fragments arranged by size:
3745 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5236
NotI GC'GGCC_GC
Cuts at: 2683 2683
Size: 5236
NspI r_CATG'y
Cuts at: 1132 1229 2407 2594 4139 1132
Size: 97 1178 187 1545 2229
Fragments arranged by size:
2229 1545 1178 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5236
PciI A'CATG_T
Cuts at: 4135 4135
Size: 5236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5236
PfoI T'CCnGG_A
Cuts at: 2064 2064
Size: 5236
PmeI GTTT'AAAC
Cuts at: 2540 2540
Size: 5236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 4818 4850 3305
Size: 32 1481 32 3691
Fragments arranged by size:
3691 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5236
PsiI TTA'TAA
Cuts at: 2806 2806
Size: 5236
PstI C_TGCA'G
Cuts at: 830 2588 830
Size: 1758 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5236
SalI G'TCGA_C
Cuts at: 1936 2578 1936
Size: 642 4594
SbfI CC_TGCA'GG
Cuts at: 2588 2588
Size: 5236
ScaI AGT'ACT
Cuts at: 3484 5196 3484
Size: 1712 3524
SfcI C'TryA_G
Cuts at: 826 1045 2584 3719 4400 4591 826
Size: 219 1539 1135 681 191 1471
Fragments arranged by size:
1539 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2428 2428
Size: 5236
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5236
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5236
SmaI CCC'GGG
Cuts at: 1872 2243 2563 1872
Size: 371 320 4545
Fragments arranged by size:
4545 371 320
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2251 3340 4241 4503 820
Size: 197 926 308 1089 901 262 1553
Fragments arranged by size:
1553 1089 926 901 308 262 197
SnaBI TAC'GTA
Cuts at: 493 3027 493
Size: 2534 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5236
SphI G_CATG'C
Cuts at: 1132 2407 2594 1132
Size: 1275 187 3774
Fragments arranged by size:
3774 1275 187
SspI AAT'ATT
Cuts at: 5 52 3160 5
Size: 47 3108 2081
Fragments arranged by size:
3108 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5236
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2675 513
Size: 552 939 93 578 3074
Fragments arranged by size:
3074 939 578 552 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2220 3263 3280 3433 3618 4063 325
Size: 1255 640 1043 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1043 640 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3482 5194 96
Size: 274 80 33 51 1443 1505 1712 138
Fragments arranged by size:
1712 1505 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5196 5228 5196
Size: 32 5204
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5236
XbaI T'CTAG_A
Cuts at: 2572 2572
Size: 5236
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5236
XmaI C'CCGG_G
Cuts at: 1870 2241 2561 1870
Size: 371 320 4545
Fragments arranged by size:
4545 371 320
XmnI GAAnn'nnTTC
Cuts at: 3365 3365
Size: 5236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3044 276
Size: 53 83 186 936 1510 2468
Fragments arranged by size:
2468 1510 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI