Restriction map for
pFN21AE1068
(Circular) (Six-base) MAPSORT of: pFN21AE1068.seq Check: 1792 from: 1 to: 6436
pFN21AE1068 6436 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2088 3406 3449 3779 1937
Size: 151 1318 43 330 4594
Fragments arranged by size:
4594 1318 330 151 43
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2130 2441 2694 3104 3140 3711 4499 5883
Size: 498 311 253 410 36 571 788 1384
Cuts at: 5883 6151 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 788 571 498 410 311 268
253 36
AfeI AGC'GCT
Cuts at: 3593 3593
Size: 6436
AflII C'TTAA_G
Cuts at: 820 1017 2165 2897 820
Size: 197 1148 732 4359
Fragments arranged by size:
4359 1148 732 197
AflIII A'CryG_T
Cuts at: 1203 2807 3401 5335 6312 1203
Size: 1604 594 1934 977 1327
Fragments arranged by size:
1934 1604 1327 977 594
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AlwNI CAG_nnn'CTG
Cuts at: 3112 3163 3562 3715 4179 5751 3112
Size: 51 399 153 464 1572 3797
Fragments arranged by size:
3797 1572 464 399 153 51
ApaI G_GGCC'C
Cuts at: 2024 2024
Size: 6436
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 2100 2242 3745 3971 4123 6232 1493
Size: 607 142 1503 226 152 2109 1697
Fragments arranged by size:
2109 1697 1503 607 226 152 142
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 3761 1870
Size: 73 1818 4545
Fragments arranged by size:
4545 1818 73
BamHI G'GATC_C
Cuts at: 3766 4133 3766
Size: 367 6069
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3757 5212 618
Size: 325 217 627 1970 1455 1842
Fragments arranged by size:
1970 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 3569 3755 1391
Size: 633 1545 186 4072
Fragments arranged by size:
4072 1545 633 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 3344 928
Size: 971 402 1043 4020
Fragments arranged by size:
4020 1043 971 402
BbvCI CC'TCA_GC
Cuts at: 3588 3588
Size: 6436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4627 4661 1903
Size: 34 2690 34 3678
Fragments arranged by size:
3678 2690 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2593 4328 5538 2593
Size: 1735 1210 3491
Fragments arranged by size:
3491 1735 1210
BclI T'GATC_A
Cuts at: 1548 2433 1548
Size: 885 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5047 136
Size: 107 122 71 631 605 3375 1525
Fragments arranged by size:
3375 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3087 6431 3087
Size: 3344 3092
BlpI GC'TnA_GC
Cuts at: 2379 3853 2379
Size: 1474 4962
Bme1580I G_kGCm'C
Cuts at: 1790 2024 3058 4497 5653 1790
Size: 234 1034 1439 1156 2573
Fragments arranged by size:
2573 1439 1156 1034 234
BmgBI CAC'GTC
Cuts at: 1318 2014 1318
Size: 696 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2028 3370 4167
Size: 416 53 83 399 24 611 1342 797
Cuts at: 4167 5125 442
Size: 958 1753
Fragments arranged by size:
1753 1342 958 797 611 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2588 2808 3586 5096 1239
Size: 832 517 220 778 1510 2579
Fragments arranged by size:
2579 1510 832 778 517 220
Bpu10I CC'TnA_GC
Cuts at: 3588 3588
Size: 6436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2659 4561 5426 5724 2659
Size: 1902 865 298 3371
Fragments arranged by size:
3371 1902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2792 5099 882
Size: 617 1293 2307 2219
Fragments arranged by size:
2307 2219 1293 617
BsaAI yAC'GTr
Cuts at: 493 1204 4227 493
Size: 711 3023 2702
Fragments arranged by size:
3023 2702 711
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4243 4625 5260 275
Size: 53 83 186 936 2710 382 635 1451
Fragments arranged by size:
2710 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4869 5267 5541 5688 6099 4869
Size: 398 274 147 411 5206
Fragments arranged by size:
5206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3506 3536 1178
Size: 30 165 30 2103 30 4078
Fragments arranged by size:
4078 2103 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3716 1186
Size: 2530 3906
BseYI C'CCAG_C
Cuts at: 3171 3611 5639 6135 3171
Size: 440 2028 496 3472
Fragments arranged by size:
3472 2028 496 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5863
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3886 4647 5675 1540
Size: 275 186 1885 761 1028 2301
Fragments arranged by size:
2301 1885 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4497 4582 5653 4497
Size: 85 1071 5280
Fragments arranged by size:
5280 1071 85
BsmI GAATG_Cn'
Cuts at: 2480 2571 3947 4040 2480
Size: 91 1376 93 4876
Fragments arranged by size:
4876 1376 93 91
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 3058 3569 3755 4497 4582 5653
Size: 399 234 1034 511 186 742 85 1071
Cuts at: 5653 1391
Size: 2174
Fragments arranged by size:
2174 1071 1034 742 511 399 234 186
85
BspEI T'CCGG_A
Cuts at: 6099 6099
Size: 6436
BspHI T'CATG_A
Cuts at: 2950 4325 6055 2950
Size: 1375 1730 3331
Fragments arranged by size:
3331 1730 1375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2569 2845 3791 844
Size: 1725 276 946 3489
Fragments arranged by size:
3489 1725 946 276
BsrBI CCG'CTC
Cuts at: 1394 1823 4323 1394
Size: 429 2500 3507
Fragments arranged by size:
3507 2500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2307 4931 5105 65
Size: 1183 1059 2624 174 1396
Fragments arranged by size:
2624 1396 1183 1059 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5080 5267 1700
Size: 110 3270 187 2869
Fragments arranged by size:
3270 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 4496 5508 4496
Size: 1012 5424
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3115 794
Size: 2321 4115
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 3087 3766 4133 4518 4535 5987 6073 6085
Size: 1115 679 367 385 17 1452 86 12
Cuts at: 6085 6431 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1115 679 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2089 2089
Size: 6436
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2199 513
Size: 552 939 36 159 4750
Fragments arranged by size:
4750 939 552 159 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5697
Fragments arranged by size:
5697 683 56
BtsI GCAGTG_nn'
Cuts at: 3056 3952 4746 4766 3056
Size: 896 794 20 4726
Fragments arranged by size:
4726 896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5443 809
Size: 507 4127 1802
Fragments arranged by size:
4127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 3883 4772
Size: 54 1425 50 275 18 501 1552 889
Cuts at: 4772 6401 8
Size: 1629 43
Fragments arranged by size:
1629 1552 1425 889 501 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 3687 4366 1353
Size: 1107 1227 679 3423
Fragments arranged by size:
3423 1227 1107 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5023 5397 5543 387
Size: 1064 138 435 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2130 2441 2588 2694 2808 3104
Size: 393 439 59 311 147 106 114 296
Cuts at: 3104 3140 3586 3711 4499 5096 5883 6151 1239
Size: 36 446 125 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 597 446 439 393 311
296 268 147 125 114 106 59 36
EcoO109I rG'GnC_Cy
Cuts at: 2021 3615 2021
Size: 1594 4842
EcoRI G'AATT_C
Cuts at: 2242 3745 4123 2242
Size: 1503 378 4555
Fragments arranged by size:
4555 1503 378
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 3154 3595 5583 1678
Size: 1476 441 1988 2531
Fragments arranged by size:
2531 1988 1476 441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 2650 2682 5077
Size: 32 135 32 800 32 246 32 2395
Cuts at: 5077 5109 5151 5183 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2395 800 246 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3780 4026 669
Size: 988 281 1842 246 3079
Fragments arranged by size:
3079 1842 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3185 748
Size: 2437 3999
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 3232 4035 3232
Size: 803 5633
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2394 3525 3661 3678 5550 5734 1305
Size: 1089 1131 136 17 1872 184 2007
Fragments arranged by size:
2007 1872 1131 1089 184 136 17
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 10
Size: 54 1425 343 501 4113
Fragments arranged by size:
4113 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4394 4753 4912 518
Size: 648 3228 359 159 2042
Fragments arranged by size:
3228 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 3852 4529 5677 5922 1606
Size: 436 1810 677 1148 245 2120
Fragments arranged by size:
2120 1810 1148 677 436 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2199 513
Size: 552 939 195 4750
Fragments arranged by size:
4750 939 552 195
NdeI CA'TA_TG
Cuts at: 387 3360 387
Size: 2973 3463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 2811 3405 3794 5339 1132
Size: 97 1582 594 389 1545 2229
Fragments arranged by size:
2229 1582 1545 594 389 97
PacI TTA_AT'TAA
Cuts at: 2322 2322
Size: 6436
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6436
PciI A'CATG_T
Cuts at: 2807 3401 5335 2807
Size: 594 1934 3908
Fragments arranged by size:
3908 1934 594
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3314 1108
Size: 2206 4230
PfoI T'CCnGG_A
Cuts at: 2095 2697 2095
Size: 602 5834
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2686 1613
Size: 1073 5363
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PspOMI G'GGCC_C
Cuts at: 2020 2020
Size: 6436
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2076 2108 2076
Size: 32 6404
PstI C_TGCA'G
Cuts at: 830 2252 2695 2842 2941 3788 830
Size: 1422 443 147 99 847 3478
Fragments arranged by size:
3478 1422 847 443 147 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
SacII CC_GC'GG
Cuts at: 2043 2043
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SapI GCTCTTCn'nnn_
Cuts at: 2460 2460
Size: 6436
SbfI CC_TGCA'GG
Cuts at: 2252 3788 2252
Size: 1536 4900
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SfcI C'TryA_G
Cuts at: 826 1045 2248 2462 2691 2838 2937 3784 4919
Size: 219 1203 214 229 147 99 847 1135
Cuts at: 4919 5600 5791 826
Size: 681 191 1471
Fragments arranged by size:
1471 1203 1135 847 681 229 219 214
191 147 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 3763 1872
Size: 1891 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 2638 2897 4540 5441 5703
Size: 197 926 222 473 259 1643 901 262
Cuts at: 5703 820
Size: 1553
Fragments arranged by size:
1643 1553 926 901 473 262 259 222
197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 2507 4360 5
Size: 47 2455 1853 2081
Fragments arranged by size:
2455 2081 1853 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6436
StyI C'CwwG_G
Cuts at: 513 1065 2004 2199 3875 513
Size: 552 939 195 1676 3074
Fragments arranged by size:
3074 1676 939 552 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4463 4480 4633 4818 5263 325
Size: 1255 2883 17 153 185 445 1498
Fragments arranged by size:
2883 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4682 6394 96
Size: 274 80 33 51 1443 2705 1712 138
Fragments arranged by size:
2705 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 6396 6428 3506
Size: 32 2858 32 3514
Fragments arranged by size:
3514 2858 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3642 1316
Size: 2326 4110
XbaI T'CTAG_A
Cuts at: 2779 3772 2779
Size: 993 5443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6436
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 3761 1870
Size: 1891 4545
XmnI GAAnn'nnTTC
Cuts at: 2454 4565 2454
Size: 2111 4325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PmlI PpuMI PspXI PvuII RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI