Restriction map for
pFN21AE1066
(Circular) (Six-base) MAPSORT of: pFN21AE1066.seq Check: 4032 from: 1 to: 6181
pFN21AE1066 6181 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3404 3404
Size: 6181
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 3524 1937
Size: 262 1325 4594
Fragments arranged by size:
4594 1325 262
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 2655 2739 4244 5628 5896 1632
Size: 807 216 84 1505 1384 268 1917
Fragments arranged by size:
1917 1505 1384 807 268 216 84
AfeI AGC'GCT
Cuts at: 2129 2129
Size: 6181
AflII C'TTAA_G
Cuts at: 820 1017 2258 2551 820
Size: 197 1241 293 4450
Fragments arranged by size:
4450 1241 293 197
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 2753 4910 2753
Size: 2157 4024
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6181
AlwNI CAG_nnn'CTG
Cuts at: 3924 5496 3924
Size: 1572 4609
ApaI G_GGCC'C
Cuts at: 2061 3186 2061
Size: 1125 5056
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 2276 2413 3002 3032 3097 3221 3490 3716
Size: 783 137 589 30 65 124 269 226
Cuts at: 3716 3868 5977 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 783 589 269 226 152 137
124 65 30
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2073 3039 3506 1870
Size: 73 88 42 966 467 4545
Fragments arranged by size:
4545 966 467 88 73 42
AvrII C'CTAG_G
Cuts at: 3169 3169
Size: 6181
BamHI G'GATC_C
Cuts at: 3229 3511 3878 3229
Size: 282 367 5532
Fragments arranged by size:
5532 367 282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2475 3502 4957 618
Size: 325 217 627 688 1027 1455 1842
Fragments arranged by size:
1842 1455 1027 688 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2032 2061 2607 3186 3500 1391
Size: 641 29 546 579 314 4072
Fragments arranged by size:
4072 641 579 546 314 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2661 928
Size: 971 762 4448
Fragments arranged by size:
4448 971 762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3423 3457 4372 4406 1903
Size: 34 1486 34 915 34 3678
Fragments arranged by size:
3678 1486 915 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4792 136
Size: 107 122 71 631 605 3120 1525
Fragments arranged by size:
3120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6176 6176
Size: 6181
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 2061 3186 3302 4242 5398 1790
Size: 271 1125 116 940 1156 2573
Fragments arranged by size:
2573 1156 1125 940 271 116
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2010 3316 3343
Size: 416 53 83 399 24 593 1306 27
Cuts at: 3343 3912 4870 442
Size: 569 958 1753
Fragments arranged by size:
1753 1306 958 593 569 416 399 83
53 27 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2680 3264 4841 1239
Size: 821 620 584 1577 2579
Fragments arranged by size:
2579 1577 821 620 584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 4306 5171 5469 3227
Size: 1079 865 298 3939
Fragments arranged by size:
3939 1079 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2646 4844 882
Size: 617 1147 2198 2219
Fragments arranged by size:
2219 2198 1147 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 3972 493
Size: 711 912 1856 2702
Fragments arranged by size:
2702 1856 912 711
BsaBI GATnn'nnATC
Cuts at: 2973 6175 2973
Size: 3202 2979
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3386 3988 4370
Size: 53 83 186 936 476 1377 602 382
Cuts at: 4370 5005 275
Size: 635 1451
Fragments arranged by size:
1451 1377 936 635 602 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4614 5012 5286 5433 5844 4614
Size: 398 274 147 411 4951
Fragments arranged by size:
4951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 5233
Fragments arranged by size:
5233 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 3224 3418 1186
Size: 1684 354 194 3949
Fragments arranged by size:
3949 1684 354 194
BseYI C'CCAG_C
Cuts at: 2847 5384 5880 2847
Size: 2537 496 3148
Fragments arranged by size:
3148 2537 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3432 1163
Size: 573 1696 3912
Fragments arranged by size:
3912 1696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2066 2212 2218 3631 4392 5420
Size: 275 186 65 146 6 1413 761 1028
Cuts at: 5420 1540
Size: 2301
Fragments arranged by size:
2301 1413 1028 761 275 186 146 65
6
BsiHKAI G_wGCw'C
Cuts at: 2470 2607 3380 4242 4327 5398 2470
Size: 137 773 862 85 1071 3253
Fragments arranged by size:
3253 1071 862 773 137 85
BsmI GAATG_Cn'
Cuts at: 3064 3692 3785 3064
Size: 628 93 5460
Fragments arranged by size:
5460 628 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2016
Size: 6181
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2061 2470 2607 3186 3302 3380
Size: 399 242 29 409 137 579 116 78
Cuts at: 3380 3500 4242 4327 5398 1391
Size: 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 409 399 242 137
120 116 85 78 29
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 4070 5800 4070
Size: 1730 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2174 3165 3404 3536 844
Size: 1330 991 239 132 3489
Fragments arranged by size:
3489 1330 991 239 132
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2187 3261 4068 1394
Size: 429 245 119 1074 807 3507
Fragments arranged by size:
3507 1074 807 429 245 119
BsrDI GCAATG_nn'
Cuts at: 65 1248 4676 4850 65
Size: 1183 3428 174 1396
Fragments arranged by size:
3428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2154 4825 5012 1700
Size: 110 344 2671 187 2869
Fragments arranged by size:
2869 2671 344 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6181
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 2522 3445 1184
Size: 1338 923 3920
Fragments arranged by size:
3920 1338 923
BstYI r'GATC_y
Cuts at: 1972 3229 3511 3878 4263 4280 5732 5818 5830
Size: 1257 282 367 385 17 1452 86 12
Cuts at: 5830 6176 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 282 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3128 3128
Size: 6181
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 3216 513
Size: 552 939 87 1125 3478
Fragments arranged by size:
3478 1125 939 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3294 3382 487
Size: 683 56 2068 88 3286
Fragments arranged by size:
3286 2068 683 88 56
BtsI GCAGTG_nn'
Cuts at: 3051 3116 3415 3697 4491 4511 3051
Size: 65 299 282 794 20 4721
Fragments arranged by size:
4721 794 299 282 65 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3460 3485 3460
Size: 25 6156
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2209 3628
Size: 54 1425 50 275 18 233 146 1419
Cuts at: 3628 4517 6146 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1419 889 275 233 146 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2063 2209 3628 1537
Size: 275 251 146 1419 4090
Fragments arranged by size:
4090 1419 275 251 146
EarI CTCTTCn'nnn_
Cuts at: 1353 4111 1353
Size: 2758 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2605 2605
Size: 6181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2439 2655 2680 2739 3264 4244
Size: 393 428 379 216 25 59 525 980
Cuts at: 4244 4841 5628 5896 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 980 787 597 525 428 393 379
268 216 59 25
EcoO109I rG'GnC_Cy
Cuts at: 2058 2077 2798 2876 3182 3183 2058
Size: 19 721 78 306 1 5056
Fragments arranged by size:
5056 721 306 78 19 1
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
EcoRV GAT'ATC
Cuts at: 3340 3340
Size: 6181
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 2039 2131 5328 1678
Size: 361 92 3197 2531
Fragments arranged by size:
3197 2531 361 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2308 2340 2494 2526 2739
Size: 32 135 32 736 32 154 32 213
Cuts at: 2739 2771 4822 4854 4896 4928 1373
Size: 32 2051 32 42 32 2626
Fragments arranged by size:
2626 2051 736 213 154 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2947 3525 3771 669
Size: 988 281 1009 578 246 3079
Fragments arranged by size:
3079 1009 988 578 281 246
HindIII A'AGCT_T
Cuts at: 748 2453 2711 3462 748
Size: 1705 258 751 3467
Fragments arranged by size:
3467 1705 751 258
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5295 5479 1305
Size: 3990 184 2007
Fragments arranged by size:
3990 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4359
Fragments arranged by size:
4359 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4139 4498 4657 518
Size: 648 2973 359 159 2042
Fragments arranged by size:
2973 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2847 2941 3597 4274 5422 5667 1606
Size: 531 710 94 656 677 1148 245 2120
Fragments arranged by size:
2120 1148 710 677 656 531 245 94
NaeI GCC'GGC
Cuts at: 2156 2156
Size: 6181
NcoI C'CATG_G
Cuts at: 513 1065 2004 3216 513
Size: 552 939 1212 3478
Fragments arranged by size:
3478 1212 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NgoMIV G'CCGG_C
Cuts at: 2154 2154
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NruI TCG'CGA
Cuts at: 3401 3401
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 3095 3539 5084 1132
Size: 97 1866 444 1545 2229
Fragments arranged by size:
2229 1866 1545 444 97
PasI CC'CwG_GG
Cuts at: 1348 2872 1348
Size: 1524 4657
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3355 1108
Size: 2247 3934
PfoI T'CCnGG_A
Cuts at: 3232 3232
Size: 6181
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2077
Size: 6181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PspOMI G'GGCC_C
Cuts at: 2057 3182 2057
Size: 1125 5056
PspXI vC'TCGA_Gb
Cuts at: 3039 3039
Size: 6181
PstI C_TGCA'G
Cuts at: 830 3436 3533 830
Size: 2606 97 3478
Fragments arranged by size:
3478 2606 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2847 2941 2847
Size: 94 6087
SacI G_AGCT'C
Cuts at: 2607 2607
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SexAI A'CCwGG_T
Cuts at: 2079 2526 3144 2079
Size: 447 618 5116
Fragments arranged by size:
5116 618 447
SfcI C'TryA_G
Cuts at: 826 1045 2842 3432 3529 4664 5345 5536 826
Size: 219 1797 590 97 1135 681 191 1471
Fragments arranged by size:
1797 1471 1135 681 590 219 191 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 2154 1700
Size: 454 5727
SmaI CCC'GGG
Cuts at: 1872 2033 2075 3508 1872
Size: 161 42 1433 4545
Fragments arranged by size:
4545 1433 161 42
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 2551 3039 3206 4285 5186
Size: 197 926 315 293 488 167 1079 901
Cuts at: 5186 5448 820
Size: 262 1553
Fragments arranged by size:
1553 1079 926 901 488 315 293 262
197 167
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SrfI GCCC'GGGC
Cuts at: 2033 2033
Size: 6181
SspI AAT'ATT
Cuts at: 5 52 3067 4105 5
Size: 47 3015 1038 2081
Fragments arranged by size:
3015 2081 1038 47
StuI AGG'CCT
Cuts at: 1517 2424 1517
Size: 907 5274
StyI C'CwwG_G
Cuts at: 513 1065 2004 2436 2801 3169 3216 3620 513
Size: 552 939 432 365 368 47 404 3074
Fragments arranged by size:
3074 939 552 432 404 368 365 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2839 3395 3409 4208 4225 4378 4563
Size: 1255 1259 556 14 799 17 153 185
Cuts at: 4563 5008 325
Size: 445 1498
Fragments arranged by size:
1498 1259 1255 799 556 445 185 153
17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4427 6139 96
Size: 274 80 33 51 1443 2450 1712 138
Fragments arranged by size:
2450 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6141 6173 6141
Size: 32 6149
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3223 3223
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 3039 1943
Size: 1096 5085
XmaI C'CCGG_G
Cuts at: 1870 2031 2073 3506 1870
Size: 161 42 1433 4545
Fragments arranged by size:
4545 1433 161 42
XmnI GAAnn'nnTTC
Cuts at: 2277 2424 4310 2277
Size: 147 1886 4148
Fragments arranged by size:
4148 1886 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI
NarI NsiI PacI PmlI PsrI RsrII SacII SanDI
SapI SfiI SfoI SwaI