Restriction map for
pFN21AE1063
(Circular) (Six-base) MAPSORT of: pFN21AE1063.seq Check: 5435 from: 1 to: 6448
pFN21AE1063 6448 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2231 2231
Size: 6448
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4258 278
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3791 1937
Size: 1854 4594
Acc65I G'GTAC_C
Cuts at: 3769 3769
Size: 6448
AclI AA'CG_TT
Cuts at: 1563 4575 4948 1563
Size: 3012 373 3063
Fragments arranged by size:
3063 3012 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2161 2369 2770 3169 3505 3589 4511 5895
Size: 529 208 401 399 336 84 922 1384
Cuts at: 5895 6163 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 922 529 401 399 336 268
208 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6251
AflIII A'CryG_T
Cuts at: 1203 2040 5347 6324 1203
Size: 837 3307 977 1327
Fragments arranged by size:
3307 1327 977 837
AgeI A'CCGG_T
Cuts at: 5279 5279
Size: 6448
AhdI GACnn_n'nnGTC
Cuts at: 5177 5177
Size: 6448
AleI CACnn'nnGTG
Cuts at: 1166 3063 1166
Size: 1897 4551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6448
AlwNI CAG_nnn'CTG
Cuts at: 2077 3675 4191 5763 2077
Size: 1598 516 1572 2762
Fragments arranged by size:
2762 1598 1572 516
ApaLI G'TGCA_C
Cuts at: 4505 5661 4505
Size: 1156 5292
ApoI r'AATT_y
Cuts at: 1493 2309 3054 3219 3471 3555 3757 3983 4135
Size: 816 745 165 252 84 202 226 152
Cuts at: 4135 6244 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 816 745 252 226 202 165
152 84
AseI AT'TA_AT
Cuts at: 160 1032 5002 160
Size: 872 3970 1606
Fragments arranged by size:
3970 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
AvaI C'yCGr_G
Cuts at: 1870 1943 3773 1870
Size: 73 1830 4545
Fragments arranged by size:
4545 1830 73
BamHI G'GATC_C
Cuts at: 3778 4145 3778
Size: 367 6081
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3769 5224 618
Size: 325 217 627 1982 1455 1842
Fragments arranged by size:
1982 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3767 1391
Size: 2376 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3269 928
Size: 971 1370 4107
Fragments arranged by size:
4107 1370 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4639 4673 1903
Size: 34 2702 34 3678
Fragments arranged by size:
3678 2702 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4340 5550 4340
Size: 1210 5238
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6448
BfrBI ATG'CAT
Cuts at: 3391 3391
Size: 6448
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5059 136
Size: 107 122 71 631 605 3387 1525
Fragments arranged by size:
3387 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6443 6443
Size: 6448
BlpI GC'TnA_GC
Cuts at: 3865 3865
Size: 6448
Bme1580I G_kGCm'C
Cuts at: 1790 4509 5665 1790
Size: 2719 1156 2573
Fragments arranged by size:
2719 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2670 3108 4179
Size: 416 53 83 399 24 1253 438 1071
Cuts at: 4179 5137 442
Size: 958 1753
Fragments arranged by size:
1753 1253 1071 958 438 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 5108 1239
Size: 842 3027 2579
Fragments arranged by size:
3027 2579 842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 4573 5438 5736 2138
Size: 2435 865 298 2850
Fragments arranged by size:
2850 2435 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3481 5111 882
Size: 617 1982 1630 2219
Fragments arranged by size:
2219 1982 1630 617
BsaAI yAC'GTr
Cuts at: 493 1204 4239 493
Size: 711 3035 2702
Fragments arranged by size:
3035 2702 711
BsaBI GATnn'nnATC
Cuts at: 6442 6442
Size: 6448
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4255 4637 5272 275
Size: 53 83 186 936 2722 382 635 1451
Fragments arranged by size:
2722 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2744 4881 5279 5553 5700 6111 2744
Size: 2137 398 274 147 411 3081
Fragments arranged by size:
3081 2137 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2415 2445 1178
Size: 30 165 30 1012 30 5181
Fragments arranged by size:
5181 1012 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 5556
BseYI C'CCAG_C
Cuts at: 5651 6147 5651
Size: 496 5952
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2259 3100 1163
Size: 573 523 841 4511
Fragments arranged by size:
4511 841 573 523
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3898 4659 5687 1540
Size: 275 186 1897 761 1028 2301
Fragments arranged by size:
2301 1897 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4509 4594 5665 4509
Size: 85 1071 5292
Fragments arranged by size:
5292 1071 85
BsmI GAATG_Cn'
Cuts at: 2327 2768 2852 3335 3393 3503 3671 3686 3959
Size: 441 84 483 58 110 168 15 273
Cuts at: 3959 4052 2327
Size: 93 4723
Fragments arranged by size:
4723 483 441 273 168 110 93 84
58 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3767 4509 4594 5665 1391
Size: 399 1977 742 85 1071 2174
Fragments arranged by size:
2174 1977 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6111 6111
Size: 6448
BspHI T'CATG_A
Cuts at: 4337 6067 4337
Size: 1730 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2231 2584 3803 844
Size: 1387 353 1219 3489
Fragments arranged by size:
3489 1387 1219 353
BsrBI CCG'CTC
Cuts at: 1394 1823 4335 1394
Size: 429 2512 3507
Fragments arranged by size:
3507 2512 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4943 5117 65
Size: 1183 3695 174 1396
Fragments arranged by size:
3695 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5092 5279 1700
Size: 110 3282 187 2869
Fragments arranged by size:
3282 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2725 3376 96
Size: 2629 651 3168
Fragments arranged by size:
3168 2629 651
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6448
BssSI C'ACGA_G
Cuts at: 4508 5520 4508
Size: 1012 5436
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6448
BstBI TT'CG_AA
Cuts at: 5252 5252
Size: 6448
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6448
BstXI CCAn_nnnn'nTGG
Cuts at: 2262 2262
Size: 6448
BstYI r'GATC_y
Cuts at: 1972 3778 4145 4530 4547 5999 6085 6097 6443
Size: 1806 367 385 17 1452 86 12 346
Cuts at: 6443 1972
Size: 1977
Fragments arranged by size:
1977 1806 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 513
Size: 552 939 138 4819
Fragments arranged by size:
4819 939 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5709
Fragments arranged by size:
5709 683 56
BtsI GCAGTG_nn'
Cuts at: 2857 3088 3964 4758 4778 2857
Size: 231 876 794 20 4527
Fragments arranged by size:
4527 876 794 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3406 3441 529
Size: 35 2842 35 3536
Fragments arranged by size:
3536 2842 35 35
DraI TTT'AAA
Cuts at: 2475 3752 2475
Size: 1277 5171
DraIII CAC_nnn'GTG
Cuts at: 2137 2137
Size: 6448
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5455 809
Size: 507 4139 1802
Fragments arranged by size:
4139 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3895 4784
Size: 54 1425 50 275 18 177 1888 889
Cuts at: 4784 6413 8
Size: 1629 43
Fragments arranged by size:
1888 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3895 1537
Size: 275 2083 4090
Fragments arranged by size:
4090 2083 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4378 1353
Size: 3025 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5035 5409 5555 387
Size: 1064 138 3446 374 146 1280
Fragments arranged by size:
3446 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2161 2369 2770 3169 3505 3589
Size: 393 449 80 208 401 399 336 84
Cuts at: 3589 4511 5108 5895 6163 1239
Size: 922 597 787 268 1524
Fragments arranged by size:
1524 922 787 597 449 401 399 393
336 268 208 84 80
EcoNI CCTnn'n_nnAGG
Cuts at: 3523 3523
Size: 6448
EcoO109I rG'GnC_Cy
Cuts at: 2220 2220
Size: 6448
EcoRI G'AATT_C
Cuts at: 3054 3219 3471 3555 3757 4135 3054
Size: 165 252 84 202 378 5367
Fragments arranged by size:
5367 378 252 202 165 84
FspI TGC'GCA
Cuts at: 4954 4954
Size: 6448
HaeII r_GCGC'y
Cuts at: 1678 5595 1678
Size: 3917 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2881 2913 2950 2982 3448
Size: 32 135 32 1309 32 37 32 466
Cuts at: 3448 3480 5089 5121 5163 5195 1373
Size: 32 1609 32 42 32 2626
Fragments arranged by size:
2626 1609 1309 466 135 42 37 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 2799 2883 3366 3534 3702
Size: 988 281 334 527 84 483 168 168
Cuts at: 3702 3792 4038 669
Size: 90 246 3079
Fragments arranged by size:
3079 988 527 483 334 281 246 168
168 90 84
HindIII A'AGCT_T
Cuts at: 748 3181 748
Size: 2433 4015
HpaI GTT'AAC
Cuts at: 4038 4038
Size: 6448
KpnI G_GTAC'C
Cuts at: 3773 3773
Size: 6448
MfeI C'AATT_G
Cuts at: 4047 4047
Size: 6448
MluI A'CGCG_T
Cuts at: 6324 6324
Size: 6448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5562 5746 1305
Size: 4257 184 2007
Fragments arranged by size:
4257 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4449
Fragments arranged by size:
4449 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2929 3063 4406 4765 4924 518
Size: 648 843 920 134 1343 359 159 2042
Fragments arranged by size:
2042 1343 920 843 648 359 159 134
MspA1I CmG'CkG
Cuts at: 1606 3864 4541 5689 5934 1606
Size: 2258 677 1148 245 2120
Fragments arranged by size:
2258 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4957
Fragments arranged by size:
4957 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6448
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6448
NotI GC'GGCC_GC
Cuts at: 3895 3895
Size: 6448
NsiI A_TGCA'T
Cuts at: 3393 3393
Size: 6448
NspI r_CATG'y
Cuts at: 1132 1229 3806 5351 1132
Size: 97 2577 1545 2229
Fragments arranged by size:
2577 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6448
PciI A'CATG_T
Cuts at: 5347 5347
Size: 6448
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6448
PfoI T'CCnGG_A
Cuts at: 2201 2201
Size: 6448
PmeI GTTT'AAAC
Cuts at: 3752 3752
Size: 6448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4517 4549 6030 6062 4517
Size: 32 1481 32 4903
Fragments arranged by size:
4903 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2220
Size: 6448
PshAI GACnn'nnGTC
Cuts at: 1613 2940 1613
Size: 1327 5121
PsiI TTA'TAA
Cuts at: 2556 3012 4018 2556
Size: 456 1006 4986
Fragments arranged by size:
4986 1006 456
PstI C_TGCA'G
Cuts at: 830 2581 3800 830
Size: 1751 1219 3478
Fragments arranged by size:
3478 1751 1219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6448
SalI G'TCGA_C
Cuts at: 1936 3790 1936
Size: 1854 4594
SanDI GG'GwC_CC
Cuts at: 2220 2220
Size: 6448
SbfI CC_TGCA'GG
Cuts at: 3800 3800
Size: 6448
ScaI AGT'ACT
Cuts at: 4696 6408 4696
Size: 1712 4736
SexAI A'CCwGG_T
Cuts at: 2133 2188 2133
Size: 55 6393
SfcI C'TryA_G
Cuts at: 826 1045 2101 2125 2577 3796 4931 5612 5803
Size: 219 1056 24 452 1219 1135 681 191
Cuts at: 5803 826
Size: 1471
Fragments arranged by size:
1471 1219 1135 1056 681 452 219 191
24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6448
SmaI CCC'GGG
Cuts at: 1872 3775 1872
Size: 1903 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 4552 5453 5715 820
Size: 197 926 174 2435 901 262 1553
Fragments arranged by size:
2435 1553 926 901 262 197 174
SnaBI TAC'GTA
Cuts at: 493 4239 493
Size: 3746 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6448
SphI G_CATG'C
Cuts at: 1132 3806 1132
Size: 2674 3774
SspI AAT'ATT
Cuts at: 5 52 2441 4372 5
Size: 47 2389 1931 2081
Fragments arranged by size:
2389 2081 1931 47
StuI AGG'CCT
Cuts at: 1517 3602 1517
Size: 2085 4363
StyI C'CwwG_G
Cuts at: 513 1065 2004 3887 513
Size: 552 939 1883 3074
Fragments arranged by size:
3074 1883 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4475 4492 4645 4830 5275 325
Size: 1255 2895 17 153 185 445 1498
Fragments arranged by size:
2895 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2681 2725 3026
Size: 274 80 33 51 1443 704 44 301
Cuts at: 3026 3376 4694 6406 96
Size: 350 1318 1712 138
Fragments arranged by size:
1712 1443 1318 704 350 301 274 138
80 51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 6408 6440 2123
Size: 32 4253 32 2131
Fragments arranged by size:
4253 2131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6448
XbaI T'CTAG_A
Cuts at: 3784 3784
Size: 6448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6448
XmaI C'CCGG_G
Cuts at: 1870 3773 1870
Size: 1903 4545
XmnI GAAnn'nnTTC
Cuts at: 3182 3266 3602 3686 4577 3182
Size: 84 336 84 891 5053
Fragments arranged by size:
5053 891 336 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4256 276
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SapI SfiI SfoI SrfI SwaI XcmI