Restriction map for
pFN21AE1061
(Circular) (Six-base) MAPSORT of: pFN21AE1061.seq Check: 5982 from: 1 to: 5602
pFN21AE1061 5602 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 2468 3729 4102 1563
Size: 905 1261 373 3063
Fragments arranged by size:
3063 1261 905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2820 2881 3665 5049 5317 1632
Size: 1188 61 784 1384 268 1917
Fragments arranged by size:
1917 1384 1188 784 268 61
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2159 1166
Size: 993 4609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2121 2461 1184
Size: 937 340 4325
Fragments arranged by size:
4325 937 340
AlwNI CAG_nnn'CTG
Cuts at: 2653 2813 3345 4917 2653
Size: 160 532 1572 3338
Fragments arranged by size:
3338 1572 532 160
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2362 2563 2911 3137 3289 5398 1493
Size: 869 201 348 226 152 2109 1697
Fragments arranged by size:
2109 1697 869 348 226 201 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 4378 618
Size: 325 217 627 1136 1455 1842
Fragments arranged by size:
1842 1455 1136 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2340 2921 1391
Size: 949 581 4072
Fragments arranged by size:
4072 949 581
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2549 928
Size: 971 650 3981
Fragments arranged by size:
3981 971 650
BbvCI CC'TCA_GC
Cuts at: 2791 2856 2791
Size: 65 5537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4213 136
Size: 107 122 71 631 605 2541 1525
Fragments arranged by size:
2541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2122 5597 2122
Size: 3475 2127
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 3663 4819 1790
Size: 1873 1156 2573
Fragments arranged by size:
2573 1873 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2381 2465 1056
Size: 1325 84 4193
Fragments arranged by size:
4193 1325 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2730 4262 1239
Size: 797 694 1532 2579
Fragments arranged by size:
2579 1532 797 694
Bpu10I CC'TnA_GC
Cuts at: 2791 2856 2791
Size: 65 5537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2401 2791 2845 2897 4265 882
Size: 617 902 390 54 52 1368 2219
Fragments arranged by size:
2219 1368 902 617 390 54 52
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2191 2221 1178
Size: 30 165 30 788 30 4559
Fragments arranged by size:
4559 788 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2196 2779 1186
Size: 1010 583 4009
Fragments arranged by size:
4009 1010 583
BseYI C'CCAG_C
Cuts at: 4805 5301 4805
Size: 496 5106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 3663 3748 4819 2340
Size: 1323 85 1071 3123
Fragments arranged by size:
3123 1323 1071 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2921 3663 3748 4819 1391
Size: 399 550 581 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 550 399 85
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 2637 3491 5221 2637
Size: 854 1730 3018
Fragments arranged by size:
3018 1730 854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2046 2513 2046
Size: 467 5135
BstYI r'GATC_y
Cuts at: 1972 2122 2493 2650 2713 2932 3299 3684 3701
Size: 150 371 157 63 219 367 385 17
Cuts at: 3701 5153 5239 5251 5597 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 371 367 346 219 157
150 86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2039 2826 513
Size: 552 939 35 787 3289
Fragments arranged by size:
3289 939 787 552 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2199 2368 3118 3912 3932 2199
Size: 169 750 794 20 3869
Fragments arranged by size:
3869 794 750 169 20
ClaI AT'CG_AT
Cuts at: 1554 2536 2751 1554
Size: 982 215 4405
Fragments arranged by size:
4405 982 215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2117 2152 529
Size: 35 1553 35 3979
Fragments arranged by size:
3979 1553 35 35
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 3049 3938
Size: 54 1425 50 275 18 246 973 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 973 889 275 246 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2900 3532 1353
Size: 1547 632 3423
Fragments arranged by size:
3423 1547 632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2338 2338
Size: 5602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2730 2820 2881 3665 4262 5049
Size: 393 404 694 90 61 784 597 787
Cuts at: 5049 5317 1239
Size: 268 1524
Fragments arranged by size:
1524 787 784 694 597 404 393 268
90 61
EcoO109I rG'GnC_Cy
Cuts at: 2840 2840
Size: 5602
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2709 4749 1678
Size: 1031 2040 2531
Fragments arranged by size:
2531 2040 1031
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2401 2433 2518 2550 2807
Size: 32 135 32 829 32 85 32 257
Cuts at: 2807 2839 4243 4275 4317 4349 1373
Size: 32 1404 32 42 32 2626
Fragments arranged by size:
2626 1404 829 257 135 85 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 10
Size: 54 1425 343 246 3534
Fragments arranged by size:
3534 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2044 2159 2443 3560 3919 4078 518
Size: 648 878 115 284 1117 359 159 2042
Fragments arranged by size:
2042 1117 878 648 359 284 159 115
MspA1I CmG'CkG
Cuts at: 1606 2860 3018 3695 4843 5088 1606
Size: 1254 158 677 1148 245 2120
Fragments arranged by size:
2120 1254 1148 677 245 158
NcoI C'CATG_G
Cuts at: 513 1065 2004 2039 513
Size: 552 939 35 4076
Fragments arranged by size:
4076 939 552 35
NdeI CA'TA_TG
Cuts at: 387 2478 387
Size: 2091 3511
NheI G'CTAG_C
Cuts at: 1052 2377 2461 1052
Size: 1325 84 4193
Fragments arranged by size:
4193 1325 84
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2377 2610 2960 4505 1132
Size: 97 1148 233 350 1545 2229
Fragments arranged by size:
2229 1545 1148 350 233 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2215 2649 2850 2954 830
Size: 1385 434 201 104 3478
Fragments arranged by size:
3478 1385 434 201 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
PvuII CAG'CTG
Cuts at: 2860 2860
Size: 5602
SacI G_AGCT'C
Cuts at: 2340 2340
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2051 2211 2645 2846 2950 4085 4766
Size: 219 1006 160 434 201 104 1135 681
Cuts at: 4766 4957 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1006 681 434 219 201 191
160 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 2156 152
Size: 2004 3598
SphI G_CATG'C
Cuts at: 1132 2377 2610 2960 1132
Size: 1245 233 350 3774
Fragments arranged by size:
3774 1245 350 233
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 2025 1517
Size: 508 5094
StyI C'CwwG_G
Cuts at: 513 1065 2004 2039 2079 2178 2885 3041 513
Size: 552 939 35 40 99 707 156 3074
Fragments arranged by size:
3074 939 707 552 156 99 40 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2191 2223 2664 2696 5562 5594 2191
Size: 32 441 32 2866 32 2199
Fragments arranged by size:
2866 2199 441 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI