Restriction map for
pFN21AE1059
(Circular) (Six-base) MAPSORT of: pFN21AE1059.seq Check: 5731 from: 1 to: 7315
pFN21AE1059 7315 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2730 2730
Size: 7315
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5125 278
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 2673 2919 3219 4658 1937
Size: 409 327 246 300 1439 4594
Fragments arranged by size:
4594 1439 409 327 300 246
Acc65I G'GTAC_C
Cuts at: 4636 4636
Size: 7315
AclI AA'CG_TT
Cuts at: 1563 5442 5815 1563
Size: 3879 373 3063
Fragments arranged by size:
3879 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3802 4127 5378 6762 7030 1632
Size: 2170 325 1251 1384 268 1917
Fragments arranged by size:
2170 1917 1384 1251 325 268
AfeI AGC'GCT
Cuts at: 2034 2034
Size: 7315
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7118
AflIII A'CryG_T
Cuts at: 1203 3084 6214 7191 1203
Size: 1881 3130 977 1327
Fragments arranged by size:
3130 1881 1327 977
AgeI A'CCGG_T
Cuts at: 6146 6146
Size: 7315
AhdI GACnn_n'nnGTC
Cuts at: 4399 6044 4399
Size: 1645 5670
AleI CACnn'nnGTG
Cuts at: 1166 2654 1166
Size: 1488 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2966 3819 1184
Size: 1782 853 4680
Fragments arranged by size:
4680 1782 853
AlwNI CAG_nnn'CTG
Cuts at: 2122 2225 2516 3514 3667 4105 4234 5058 6630
Size: 103 291 998 153 438 129 824 1572
Cuts at: 6630 2122
Size: 2807
Fragments arranged by size:
2807 1572 998 824 438 291 153 129
103
ApaI G_GGCC'C
Cuts at: 4218 4218
Size: 7315
ApaLI G'TGCA_C
Cuts at: 3656 5372 6528 3656
Size: 1716 1156 4443
Fragments arranged by size:
4443 1716 1156
ApoI r'AATT_y
Cuts at: 1493 4624 4850 5002 7111 1493
Size: 3131 226 152 2109 1697
Fragments arranged by size:
3131 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5869 160
Size: 872 4837 1606
Fragments arranged by size:
4837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
AvaI C'yCGr_G
Cuts at: 1870 1943 2395 2731 3151 3764 3841 4192 4640
Size: 73 452 336 420 613 77 351 448
Cuts at: 4640 1870
Size: 4545
Fragments arranged by size:
4545 613 452 448 420 351 336 77
73
AvrII C'CTAG_G
Cuts at: 4594 4594
Size: 7315
BamHI G'GATC_C
Cuts at: 4645 5012 4645
Size: 367 6948
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2154 2353 2503 2545 2644
Size: 325 217 627 367 199 150 42 99
Cuts at: 2644 3155 3238 3860 4636 6091 618
Size: 511 83 622 776 1455 1842
Fragments arranged by size:
1842 1455 776 627 622 511 367 325
217 199 150 99 83 42
BanII G_rGCy'C
Cuts at: 1391 2070 4218 4634 1391
Size: 679 2148 416 4072
Fragments arranged by size:
4072 2148 679 416
BbeI G_GCGC'C
Cuts at: 2158 2158
Size: 7315
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4040 928
Size: 971 2141 4203
Fragments arranged by size:
4203 2141 971
BbvCI CC'TCA_GC
Cuts at: 2259 4364 2259
Size: 2105 5210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5506 5540 1903
Size: 34 3569 34 3678
Fragments arranged by size:
3678 3569 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5207 6417 5207
Size: 1210 6105
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2127 2221 2249
Size: 107 122 71 631 605 455 94 28
Cuts at: 2249 2512 2790 3705 3955 5926 136
Size: 263 278 915 250 1971 1525
Fragments arranged by size:
1971 1525 915 631 605 455 278 263
250 122 107 94 71 28
BglII A'GATC_T
Cuts at: 2187 4332 7310 2187
Size: 2145 2978 2192
Fragments arranged by size:
2978 2192 2145
BlpI GC'TnA_GC
Cuts at: 2373 3626 4732 2373
Size: 1253 1106 4956
Fragments arranged by size:
4956 1253 1106
Bme1580I G_kGCm'C
Cuts at: 1790 2358 2508 2621 2649 2760 2874 2955 2994
Size: 568 150 113 28 111 114 81 39
Cuts at: 2994 3129 3158 3174 3613 3660 3765 4218 5376
Size: 135 29 16 439 47 105 453 1158
Cuts at: 5376 6532 1790
Size: 1156 2573
Fragments arranged by size:
2573 1158 1156 568 453 439 150 135
114 113 111 105 81 47 39 29
28 16
BmgBI CAC'GTC
Cuts at: 1318 2869 3085 3094 3124 3655 4426 1318
Size: 1551 216 9 30 531 771 4207
Fragments arranged by size:
4207 1551 771 531 216 30 9
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 2871 3474
Size: 416 53 83 399 24 873 581 603
Cuts at: 3474 5046 6004 442
Size: 1572 958 1753
Fragments arranged by size:
1753 1572 958 873 603 581 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2538 2876 3045 3277 3386 3732 4112
Size: 884 415 338 169 232 109 346 380
Cuts at: 4112 4268 5975 1239
Size: 156 1707 2579
Fragments arranged by size:
2579 1707 884 415 380 346 338 232
169 156 109
Bpu10I CC'TnA_GC
Cuts at: 2259 4364 2259
Size: 2105 5210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3743 5440 6305 6603 2168
Size: 1575 1697 865 298 2880
Fragments arranged by size:
2880 1697 1575 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2838 2972 5978 882
Size: 617 1339 134 3006 2219
Fragments arranged by size:
3006 2219 1339 617 134
BsaAI yAC'GTr
Cuts at: 493 1204 5106 493
Size: 711 3902 2702
Fragments arranged by size:
3902 2702 711
BsaBI GATnn'nnATC
Cuts at: 7309 7309
Size: 7315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2155 3771 4172 4293
Size: 53 83 186 936 622 1616 401 121
Cuts at: 4293 5122 5504 6139 275
Size: 829 382 635 1451
Fragments arranged by size:
1616 1451 936 829 635 622 401 382
186 121 83 53
BsaWI w'CCGG_w
Cuts at: 5748 6146 6420 6567 6978 5748
Size: 398 274 147 411 6085
Fragments arranged by size:
6085 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3460 3490 1178
Size: 30 165 30 2057 30 5003
Fragments arranged by size:
5003 2057 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2990 4037 1186
Size: 1804 1047 4464
Fragments arranged by size:
4464 1804 1047
BseYI C'CCAG_C
Cuts at: 2253 2278 2331 2363 2402 2425 2477 2610 2685
Size: 25 53 32 39 23 52 133 75
Cuts at: 2685 2780 3247 3265 4561 4575 6518 7014 2253
Size: 95 467 18 1296 14 1943 496 2554
Fragments arranged by size:
2554 1943 1296 496 467 133 95 75
53 52 39 32 25 23 18 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3527 3779 3851 1163
Size: 573 1791 252 72 4627
Fragments arranged by size:
4627 1791 573 252 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3078 4765 5526 6554 1540
Size: 275 186 1077 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1077 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 3660 5376 5461 6532 2070
Size: 1590 1716 85 1071 2853
Fragments arranged by size:
2853 1716 1590 1071 85
BsmI GAATG_Cn'
Cuts at: 4826 4919 4826
Size: 93 7222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2358 2508 2621 2649 2760 2874
Size: 399 280 288 150 113 28 111 114
Cuts at: 2874 2955 2994 3129 3158 3174 3613 3660 3765
Size: 81 39 135 29 16 439 47 105
Cuts at: 3765 4218 4634 5376 5461 6532 1391
Size: 453 416 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 439 416 399 288
280 150 135 114 113 111 105 85
81 47 39 29 28 16
BspEI T'CCGG_A
Cuts at: 6978 6978
Size: 7315
BspHI T'CATG_A
Cuts at: 2091 5204 6934 2091
Size: 3113 1730 2472
Fragments arranged by size:
3113 2472 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2730 3522 4464 4670 844
Size: 1886 792 942 206 3489
Fragments arranged by size:
3489 1886 942 792 206
BsrBI CCG'CTC
Cuts at: 1394 1823 5202 1394
Size: 429 3379 3507
Fragments arranged by size:
3507 3379 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3592 3808 5810 5984 65
Size: 1183 2344 216 2002 174 1396
Fragments arranged by size:
2344 2002 1396 1183 216 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3073 3299 3455 3630 3735 4224 5959
Size: 110 1263 226 156 175 105 489 1735
Cuts at: 5959 6146 1700
Size: 187 2869
Fragments arranged by size:
2869 1735 1263 489 226 187 175 156
110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7315
BssSI C'ACGA_G
Cuts at: 3551 5375 6387 3551
Size: 1824 1012 4479
Fragments arranged by size:
4479 1824 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3514 4234 794
Size: 2720 720 3875
Fragments arranged by size:
3875 2720 720
BstBI TT'CG_AA
Cuts at: 6119 6119
Size: 7315
BstEII G'GTnAC_C
Cuts at: 1184 4438 1184
Size: 3254 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 3781 4288 2656
Size: 1125 507 5683
Fragments arranged by size:
5683 1125 507
BstYI r'GATC_y
Cuts at: 1972 2187 4332 4645 5012 5397 5414 6866 6952
Size: 215 2145 313 367 385 17 1452 86
Cuts at: 6952 6964 7310 1972
Size: 12 346 1977
Fragments arranged by size:
2145 1977 1452 385 367 346 313 215
86 17 12
BstZ17I GTA'TAC
Cuts at: 2674 2674
Size: 7315
Bsu36I CC'TnA_GG
Cuts at: 3195 3849 3195
Size: 654 6661
BtgI C'CryG_G
Cuts at: 513 1065 2595 2817 4287 4308 4527 513
Size: 552 1530 222 1470 21 219 3301
Fragments arranged by size:
3301 1530 1470 552 222 219 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4503 487
Size: 683 56 3277 3299
Fragments arranged by size:
3299 3277 683 56
BtsI GCAGTG_nn'
Cuts at: 2963 3809 3834 4259 4831 5625 5645 2963
Size: 846 25 425 572 794 20 4633
Fragments arranged by size:
4633 846 794 572 425 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2622 2657 529
Size: 35 2058 35 5187
Fragments arranged by size:
5187 2058 35 35
DraI TTT'AAA
Cuts at: 4619 4619
Size: 7315
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6322 809
Size: 507 5006 1802
Fragments arranged by size:
5006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2049 2484
Size: 54 1425 50 275 18 195 24 435
Cuts at: 2484 2791 2839 3075 3528 3697 4161 4311 4485
Size: 307 48 236 453 169 464 150 174
Cuts at: 4485 4762 5651 7280 8
Size: 277 889 1629 43
Fragments arranged by size:
1629 1425 889 464 453 435 307 277
275 236 195 174 169 150 54 50
48 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3075 4762 1537
Size: 275 1263 1687 4090
Fragments arranged by size:
4090 1687 1263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3821 4244 5245 1353
Size: 2468 423 1001 3423
Fragments arranged by size:
3423 2468 1001 423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4574 5902 6276 6422 387
Size: 1064 138 2985 1328 374 146 1280
Fragments arranged by size:
2985 1328 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2068
Size: 7315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2538 2876 3045 3277 3386 3732
Size: 393 491 415 338 169 232 109 346
Cuts at: 3732 3802 4112 4127 4268 5378 5975 6762 7030
Size: 70 310 15 141 1110 597 787 268
Cuts at: 7030 1239
Size: 1524
Fragments arranged by size:
1524 1110 787 597 491 415 393 346
338 310 268 232 169 141 109 70
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 2498
Size: 7315
EcoO109I rG'GnC_Cy
Cuts at: 2119 2222 2399 2513 2522 2584 2831 2890 3388
Size: 103 177 114 9 62 247 59 498
Cuts at: 3388 3511 3685 3727 3845 4214 4215 2119
Size: 123 174 42 118 369 1 5219
Fragments arranged by size:
5219 498 369 247 177 174 123 118
114 103 62 59 42 9 1
EcoRI G'AATT_C
Cuts at: 4624 5002 4624
Size: 378 6937
FspI TGC'GCA
Cuts at: 4430 5821 4430
Size: 1391 5924
HaeII r_GCGC'y
Cuts at: 1678 2036 2158 2285 3283 3420 3456 4371 6462
Size: 358 122 127 998 137 36 915 2091
Cuts at: 6462 1678
Size: 2531
Fragments arranged by size:
2531 2091 998 915 358 137 127 122
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2449 2481 4385
Size: 32 135 32 482 32 363 32 1904
Cuts at: 4385 4417 5956 5988 6030 6062 1373
Size: 32 1539 32 42 32 2626
Fragments arranged by size:
2626 1904 1539 482 363 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3220 4659 4905 669
Size: 988 281 1282 1439 246 3079
Fragments arranged by size:
3079 1439 1282 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3623 748
Size: 2875 4440
HpaI GTT'AAC
Cuts at: 4905 4905
Size: 7315
KasI G'GCGC_C
Cuts at: 2154 2154
Size: 7315
KpnI G_GTAC'C
Cuts at: 4640 4640
Size: 7315
MfeI C'AATT_G
Cuts at: 4914 4914
Size: 7315
MluI A'CGCG_T
Cuts at: 7191 7191
Size: 7315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2928 3540 6429 6613 1305
Size: 1623 612 2889 184 2007
Fragments arranged by size:
2889 2007 1623 612 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2793 4163 4313 4487
Size: 54 1425 343 195 766 1370 150 174
Cuts at: 4487 10
Size: 2838
Fragments arranged by size:
2838 1425 1370 766 343 195 174 150
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 2306 2654 3801 5273 5632 5791
Size: 648 930 210 348 1147 1472 359 159
Cuts at: 5791 518
Size: 2042
Fragments arranged by size:
2042 1472 1147 930 648 359 348 210
159
MspA1I CmG'CkG
Cuts at: 1606 2610 3251 3502 3637 3781 4090 4105 4231
Size: 1004 641 251 135 144 309 15 126
Cuts at: 4231 4502 4529 4603 4731 5408 6556 6801 1606
Size: 271 27 74 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 1004 677 641 309 271 251
245 144 135 128 126 74 27 15
NaeI GCC'GGC
Cuts at: 3075 3632 3075
Size: 557 6758
NarI GG'CG_CC
Cuts at: 2155 2155
Size: 7315
NcoI C'CATG_G
Cuts at: 513 1065 2595 2817 513
Size: 552 1530 222 5011
Fragments arranged by size:
5011 1530 552 222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7315
NgoMIV G'CCGG_C
Cuts at: 3073 3630 3073
Size: 557 6758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7315
NotI GC'GGCC_GC
Cuts at: 4762 4762
Size: 7315
NspI r_CATG'y
Cuts at: 1132 1229 2203 3620 4673 6218 1132
Size: 97 974 1417 1053 1545 2229
Fragments arranged by size:
2229 1545 1417 1053 974 97
PasI CC'CwG_GG
Cuts at: 1348 2510 1348
Size: 1162 6153
PciI A'CATG_T
Cuts at: 6214 6214
Size: 7315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2516 3436 3667 4159 4186 4378 4540 1108
Size: 1408 920 231 492 27 192 162 3883
Fragments arranged by size:
3883 1408 920 492 231 192 162 27
PmeI GTTT'AAAC
Cuts at: 4619 4619
Size: 7315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5384 5416 6897 6929 5384
Size: 32 1481 32 5770
Fragments arranged by size:
5770 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2399 2584 2831 3511 3727 2399
Size: 185 247 680 216 5987
Fragments arranged by size:
5987 680 247 216 185
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7315
PsiI TTA'TAA
Cuts at: 4885 4885
Size: 7315
PspOMI G'GGCC_C
Cuts at: 4214 4214
Size: 7315
PstI C_TGCA'G
Cuts at: 830 3405 3519 3642 4667 830
Size: 2575 114 123 1025 3478
Fragments arranged by size:
3478 2575 1025 123 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7315
PvuII CAG'CTG
Cuts at: 2610 3251 3637 3781 4090 4105 4231 4603 2610
Size: 641 386 144 309 15 126 372 5322
Fragments arranged by size:
5322 641 386 372 309 144 126 15
RsrII CG'GwC_CG
Cuts at: 4222 4222
Size: 7315
SacI G_AGCT'C
Cuts at: 2070 2070
Size: 7315
SacII CC_GC'GG
Cuts at: 4530 4530
Size: 7315
SalI G'TCGA_C
Cuts at: 1936 3218 4657 1936
Size: 1282 1439 4594
Fragments arranged by size:
4594 1439 1282
SanDI GG'GwC_CC
Cuts at: 2399 2831 2399
Size: 432 6883
SapI GCTCTTCn'nnn_
Cuts at: 3821 4244 3821
Size: 423 6892
SbfI CC_TGCA'GG
Cuts at: 3519 4667 3519
Size: 1148 6167
ScaI AGT'ACT
Cuts at: 5563 7275 5563
Size: 1712 5603
SexAI A'CCwGG_T
Cuts at: 2349 2499 2541 2580 2349
Size: 150 42 39 7084
Fragments arranged by size:
7084 150 42 39
SfcI C'TryA_G
Cuts at: 826 1045 3401 3515 3638 4010 4447 4663 5798
Size: 219 2356 114 123 372 437 216 1135
Cuts at: 5798 6479 6670 826
Size: 681 191 1471
Fragments arranged by size:
2356 1471 1135 681 437 372 219 216
191 123 114
SfoI GGC'GCC
Cuts at: 2156 2156
Size: 7315
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
SgrAI Cr'CCGG_yG
Cuts at: 1700 3455 1700
Size: 1755 5560
SmaI CCC'GGG
Cuts at: 1872 2397 2733 3153 3843 4194 4642 1872
Size: 525 336 420 690 351 448 4545
Fragments arranged by size:
4545 690 525 448 420 351 336
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 3722 5419 6320 6582 820
Size: 197 926 240 1539 1697 901 262 1553
Fragments arranged by size:
1697 1553 1539 926 901 262 240 197
SnaBI TAC'GTA
Cuts at: 493 5106 493
Size: 4613 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7315
SphI G_CATG'C
Cuts at: 1132 3620 4673 1132
Size: 2488 1053 3774
Fragments arranged by size:
3774 2488 1053
SrfI GCCC'GGGC
Cuts at: 3153 3153
Size: 7315
SspI AAT'ATT
Cuts at: 5 52 5239 5
Size: 47 5187 2081
Fragments arranged by size:
5187 2081 47
StuI AGG'CCT
Cuts at: 1517 2382 1517
Size: 865 6450
StyI C'CwwG_G
Cuts at: 513 1065 2595 2817 3006 3333 3828 4413 4494
Size: 552 1530 222 189 327 495 585 81
Cuts at: 4494 4594 4754 513
Size: 100 160 3074
Fragments arranged by size:
3074 1530 585 552 495 327 222 189
160 100 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4589 5342 5359 5512 5697 6142 325
Size: 1255 3009 753 17 153 185 445 1498
Fragments arranged by size:
3009 1498 1255 753 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 5561 7273
Size: 274 80 33 51 1443 321 3263 1712
Cuts at: 7273 96
Size: 138
Fragments arranged by size:
3263 1712 1443 321 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2149 7275 7307 2117
Size: 32 5126 32 2125
Fragments arranged by size:
5126 2125 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2342 4001 1316
Size: 1026 1659 4630
Fragments arranged by size:
4630 1659 1026
XbaI T'CTAG_A
Cuts at: 4651 4651
Size: 7315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2261 4336 2261
Size: 2075 5240
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7315
XmaI C'CCGG_G
Cuts at: 1870 2395 2731 3151 3841 4192 4640 1870
Size: 525 336 420 690 351 448 4545
Fragments arranged by size:
4545 690 525 448 420 351 336
XmnI GAAnn'nnTTC
Cuts at: 5444 5444
Size: 7315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5123 276
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsiWI BsmBI DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI SfiI SwaI