Restriction map for
pFN21AE1056
(Circular) (Six-base) MAPSORT of: pFN21AE1056.seq Check: 9258 from: 1 to: 8515
pFN21AE1056 8515 bp
With 182 enzymes: SgfI *
February 1, 2012 16:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5630 5630
Size: 8515
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6325 278
Size: 53 83 186 936 4789 2468
Fragments arranged by size:
4789 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5858 1937
Size: 3921 4594
Acc65I G'GTAC_C
Cuts at: 5836 5836
Size: 8515
AclI AA'CG_TT
Cuts at: 1563 6642 7015 1563
Size: 5079 373 3063
Fragments arranged by size:
5079 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 2928 3630 5070 6578 7962 8230 1632
Size: 696 600 702 1440 1508 1384 268 1917
Fragments arranged by size:
1917 1508 1440 1384 702 696 600 268
AflII C'TTAA_G
Cuts at: 820 1017 3926 820
Size: 197 2909 5409
Fragments arranged by size:
5409 2909 197
AflIII A'CryG_T
Cuts at: 1203 7414 8391 1203
Size: 6211 977 1327
Fragments arranged by size:
6211 1327 977
AgeI A'CCGG_T
Cuts at: 7346 7346
Size: 8515
AhdI GACnn_n'nnGTC
Cuts at: 7244 7244
Size: 8515
AleI CACnn'nnGTG
Cuts at: 1166 2609 1166
Size: 1443 7072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4422 1184
Size: 3238 5277
AlwNI CAG_nnn'CTG
Cuts at: 5435 6258 7830 5435
Size: 823 1572 6120
Fragments arranged by size:
6120 1572 823
ApaI G_GGCC'C
Cuts at: 2415 2415
Size: 8515
ApaLI G'TGCA_C
Cuts at: 6572 7728 6572
Size: 1156 7359
ApoI r'AATT_y
Cuts at: 1493 2020 2102 2152 3179 3214 4720 5824 6050
Size: 527 82 50 1027 35 1506 1104 226
Cuts at: 6050 6202 8311 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1506 1104 1027 527 226 152
82 50 35
AseI AT'TA_AT
Cuts at: 160 1032 3558 4899 7069 160
Size: 872 2526 1341 2170 1606
Fragments arranged by size:
2526 2170 1606 1341 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8515
AvaI C'yCGr_G
Cuts at: 1870 1943 4077 5157 5670 5840 1870
Size: 73 2134 1080 513 170 4545
Fragments arranged by size:
4545 2134 1080 513 170 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2738 2771 2738
Size: 33 8482
BamHI G'GATC_C
Cuts at: 5845 6212 5845
Size: 367 8148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5836 7291 618
Size: 325 217 627 4049 1455 1842
Fragments arranged by size:
4049 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2415 4345 5834 1391
Size: 1024 1930 1489 4072
Fragments arranged by size:
4072 1930 1489 1024
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3738 928
Size: 971 1839 5705
Fragments arranged by size:
5705 1839 971
BbvCI CC'TCA_GC
Cuts at: 5428 5428
Size: 8515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6706 6740 1903
Size: 34 4769 34 3678
Fragments arranged by size:
4769 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6407 7617 6407
Size: 1210 7305
BclI T'GATC_A
Cuts at: 1548 2620 4744 1548
Size: 1072 2124 5319
Fragments arranged by size:
5319 2124 1072
BfrBI ATG'CAT
Cuts at: 4526 4526
Size: 8515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5327 7126 136
Size: 107 122 71 631 605 3655 1799 1525
Fragments arranged by size:
3655 1799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5415 8510 5415
Size: 3095 5420
BlpI GC'TnA_GC
Cuts at: 5932 5932
Size: 8515
Bme1580I G_kGCm'C
Cuts at: 1790 2415 2734 6576 7732 1790
Size: 625 319 3842 1156 2573
Fragments arranged by size:
3842 2573 1156 625 319
BmgBI CAC'GTC
Cuts at: 1318 5483 1318
Size: 4165 4350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3265 3704 6246
Size: 416 53 83 399 24 1848 439 2542
Cuts at: 6246 7204 442
Size: 958 1753
Fragments arranged by size:
2542 1848 1753 958 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3894 3894
Size: 8515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3057 3975 5715 7175 1239
Size: 1818 918 1740 1460 2579
Fragments arranged by size:
2579 1818 1740 1460 918
Bpu10I CC'TnA_GC
Cuts at: 5428 5428
Size: 8515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2437 2826 2909 3080 4298 6640 7505 7803 2437
Size: 389 83 171 1218 2342 865 298 3149
Fragments arranged by size:
3149 2342 1218 865 389 298 171 83
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5315 7178 882
Size: 617 3816 1863 2219
Fragments arranged by size:
3816 2219 1863 617
BsaAI yAC'GTr
Cuts at: 493 1204 4630 4750 6306 493
Size: 711 3426 120 1556 2702
Fragments arranged by size:
3426 2702 1556 711 120
BsaBI GATnn'nnATC
Cuts at: 8509 8509
Size: 8515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6322 6704 7339 275
Size: 53 83 186 936 4789 382 635 1451
Fragments arranged by size:
4789 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2783 6948 7346 7620 7767 8178 2783
Size: 4165 398 274 147 411 3120
Fragments arranged by size:
4165 3120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2269 2299 2799 2829 3146
Size: 30 165 30 866 30 500 30 317
Cuts at: 3146 3176 1178
Size: 30 6517
Fragments arranged by size:
6517 866 500 317 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2442 3402 5676 5712 5766 5814 1186
Size: 1256 960 2274 36 54 48 3887
Fragments arranged by size:
3887 2274 1256 960 54 48 36
BseYI C'CCAG_C
Cuts at: 2513 2668 4702 7718 8214 2513
Size: 155 2034 3016 496 2814
Fragments arranged by size:
3016 2814 2034 496 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4354 5658 1163
Size: 573 2618 1304 4020
Fragments arranged by size:
4020 2618 1304 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5965 6726 7754 1540
Size: 275 186 3964 761 1028 2301
Fragments arranged by size:
3964 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 4345 4830 6576 6661 7732 3486
Size: 859 485 1746 85 1071 4269
Fragments arranged by size:
4269 1746 1071 859 485 85
BsmI GAATG_Cn'
Cuts at: 3507 3738 4063 6026 6119 3507
Size: 231 325 1963 93 5903
Fragments arranged by size:
5903 1963 325 231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2734 3486 4345 4830 5834 6576
Size: 399 625 319 752 859 485 1004 742
Cuts at: 6576 6661 7732 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1004 859 752 742 625 485
399 319 85
BspEI T'CCGG_A
Cuts at: 2783 8178 2783
Size: 5395 3120
BspHI T'CATG_A
Cuts at: 3130 5284 6404 8134 3130
Size: 2154 1120 1730 3511
Fragments arranged by size:
3511 2154 1730 1120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4673 5365 5630 5870 844
Size: 3829 692 265 240 3489
Fragments arranged by size:
3829 3489 692 265 240
BsrBI CCG'CTC
Cuts at: 1394 1823 6402 1394
Size: 429 4579 3507
Fragments arranged by size:
4579 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2188 4394 4650 7010 7184 65
Size: 1183 940 2206 256 2360 174 1396
Fragments arranged by size:
2360 2206 1396 1183 940 256 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7159 7346 1700
Size: 110 5349 187 2869
Fragments arranged by size:
5349 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8515
BssSI C'ACGA_G
Cuts at: 2688 6575 7587 2688
Size: 3887 1012 3616
Fragments arranged by size:
3887 3616 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2860 4532 5248 794
Size: 2066 1672 716 4061
Fragments arranged by size:
4061 2066 1672 716
BstBI TT'CG_AA
Cuts at: 2215 2958 5152 7319 2215
Size: 743 2194 2167 3411
Fragments arranged by size:
3411 2194 2167 743
BstEII G'GTnAC_C
Cuts at: 1184 3000 1184
Size: 1816 6699
BstXI CCAn_nnnn'nTGG
Cuts at: 5237 5237
Size: 8515
BstYI r'GATC_y
Cuts at: 1972 2583 2911 5415 5732 5845 6212 6597 6614
Size: 611 328 2504 317 113 367 385 17
Cuts at: 6614 8066 8152 8164 8510 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2504 1977 1452 611 385 367 346 328
317 113 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 5322 513
Size: 552 939 324 2994 3706
Fragments arranged by size:
3706 2994 939 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7776
Fragments arranged by size:
7776 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 4221 5581 6031 6825 6845 2085
Size: 2136 1360 450 794 20 3755
Fragments arranged by size:
3755 2136 1360 794 450 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4129 4164 529
Size: 35 3565 35 4880
Fragments arranged by size:
4880 3565 35 35
DraI TTT'AAA
Cuts at: 2508 5819 2508
Size: 3311 5204
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7522 809
Size: 507 6206 1802
Fragments arranged by size:
6206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4300 4444 4455
Size: 54 1425 50 275 18 2470 144 11
Cuts at: 4455 5962 6851 8480 8
Size: 1507 889 1629 43
Fragments arranged by size:
2470 1629 1507 1425 889 275 144 54
50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 5962 1537
Size: 275 4150 4090
Fragments arranged by size:
4150 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 3387 4223 5791 6445 1353
Size: 993 1041 836 1568 654 3423
Fragments arranged by size:
3423 1568 1041 993 836 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2256 7102 7476 7622 387
Size: 1064 138 667 4846 374 146 1280
Fragments arranged by size:
4846 1280 1064 667 374 146 138
EcoICRI GAG'CTC
Cuts at: 4343 4343
Size: 8515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2928 3057 3630 3975 5070 5715
Size: 393 696 600 129 573 345 1095 645
Cuts at: 5715 6578 7175 7962 8230 1239
Size: 863 597 787 268 1524
Fragments arranged by size:
1524 1095 863 787 696 645 600 597
573 393 345 268 129
EcoNI CCTnn'n_nnAGG
Cuts at: 5718 5718
Size: 8515
EcoO109I rG'GnC_Cy
Cuts at: 2336 2388 2615 3053 5711 5766 2336
Size: 52 227 438 2658 55 5085
Fragments arranged by size:
5085 2658 438 227 55 52
EcoRI G'AATT_C
Cuts at: 5824 6202 5824
Size: 378 8137
FspI TGC'GCA
Cuts at: 7021 7021
Size: 8515
HaeII r_GCGC'y
Cuts at: 1678 5613 7662 1678
Size: 3935 2049 2531
Fragments arranged by size:
3935 2531 2049
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 4626 4658 5140
Size: 32 135 32 863 32 2159 32 482
Cuts at: 5140 5172 7156 7188 7230 7262 1373
Size: 32 1984 32 42 32 2626
Fragments arranged by size:
2626 2159 1984 863 482 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2203 4548 4929 5859 6105 669
Size: 988 281 265 2345 381 930 246 3079
Fragments arranged by size:
3079 2345 988 930 381 281 265 246
HindIII A'AGCT_T
Cuts at: 748 2775 748
Size: 2027 6488
HpaI GTT'AAC
Cuts at: 2203 6105 2203
Size: 3902 4613
KpnI G_GTAC'C
Cuts at: 5840 5840
Size: 8515
MfeI C'AATT_G
Cuts at: 3673 3881 4464 6114 3673
Size: 208 583 1650 6074
Fragments arranged by size:
6074 1650 583 208
MluI A'CGCG_T
Cuts at: 8391 8391
Size: 8515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3039 3099 4096 5355 7629 7813 1305
Size: 1734 60 997 1259 2274 184 2007
Fragments arranged by size:
2274 2007 1734 1259 997 184 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4446 4457 10
Size: 54 1425 343 2614 11 4068
Fragments arranged by size:
4068 2614 1425 343 54 11
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2609 3743 6473 6832 6991 518
Size: 648 978 465 1134 2730 359 159 2042
Fragments arranged by size:
2730 2042 1134 978 648 465 359 159
MspA1I CmG'CkG
Cuts at: 1606 2668 2860 5432 5931 6608 7756 8001 1606
Size: 1062 192 2572 499 677 1148 245 2120
Fragments arranged by size:
2572 2120 1148 1062 677 499 245 192
NcoI C'CATG_G
Cuts at: 513 1065 2004 2328 5322 513
Size: 552 939 324 2994 3706
Fragments arranged by size:
3706 2994 939 552 324
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8515
NotI GC'GGCC_GC
Cuts at: 5962 5962
Size: 8515
NsiI A_TGCA'T
Cuts at: 4528 4528
Size: 8515
NspI r_CATG'y
Cuts at: 1132 1229 3655 3877 5873 7418 1132
Size: 97 2426 222 1996 1545 2229
Fragments arranged by size:
2426 2229 1996 1545 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8515
PciI A'CATG_T
Cuts at: 7414 7414
Size: 8515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2252 4031 4453 1108
Size: 1144 1779 422 5170
Fragments arranged by size:
5170 1779 1144 422
PfoI T'CCnGG_A
Cuts at: 3952 3952
Size: 8515
PmeI GTTT'AAAC
Cuts at: 5819 5819
Size: 8515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6584 6616 8097 8129 6584
Size: 32 1481 32 6970
Fragments arranged by size:
6970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2336 2615 2336
Size: 279 8236
PshAI GACnn'nnGTC
Cuts at: 1613 5186 1613
Size: 3573 4942
PsiI TTA'TAA
Cuts at: 2133 6085 2133
Size: 3952 4563
PspOMI G'GGCC_C
Cuts at: 2411 2411
Size: 8515
PspXI vC'TCGA_Gb
Cuts at: 5157 5157
Size: 8515
PstI C_TGCA'G
Cuts at: 830 5202 5867 830
Size: 4372 665 3478
Fragments arranged by size:
4372 3478 665
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8515
PvuII CAG'CTG
Cuts at: 2668 2860 5432 2668
Size: 192 2572 5751
Fragments arranged by size:
5751 2572 192
SacI G_AGCT'C
Cuts at: 4345 4345
Size: 8515
SalI G'TCGA_C
Cuts at: 1936 5857 1936
Size: 3921 4594
SapI GCTCTTCn'nnn_
Cuts at: 3387 3387
Size: 8515
SbfI CC_TGCA'GG
Cuts at: 5867 5867
Size: 8515
ScaI AGT'ACT
Cuts at: 3582 6763 8475 3582
Size: 3181 1712 3622
Fragments arranged by size:
3622 3181 1712
SfcI C'TryA_G
Cuts at: 826 1045 2295 3415 3474 5198 5569 5656 5704
Size: 219 1250 1120 59 1724 371 87 48
Cuts at: 5704 5725 5863 6998 7679 7870 826
Size: 21 138 1135 681 191 1471
Fragments arranged by size:
1724 1471 1250 1135 1120 681 371 219
191 138 87 59 48 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8515
SmaI CCC'GGG
Cuts at: 1872 5842 1872
Size: 3970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2452 2841 2888 3095 3926 4077
Size: 197 926 509 389 47 207 831 151
Cuts at: 4077 4277 5157 6619 7520 7782 820
Size: 200 880 1462 901 262 1553
Fragments arranged by size:
1553 1462 926 901 880 831 509 389
262 207 200 197 151 47
SnaBI TAC'GTA
Cuts at: 493 6306 493
Size: 5813 2702
SpeI A'CTAG_T
Cuts at: 152 3032 152
Size: 2880 5635
SphI G_CATG'C
Cuts at: 1132 5873 1132
Size: 4741 3774
SspI AAT'ATT
Cuts at: 5 52 2567 3665 3722 4147 6439 5
Size: 47 2515 1098 57 425 2292 2081
Fragments arranged by size:
2515 2292 2081 1098 425 57 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2328 2535 2587 2996 3026 4125
Size: 552 939 324 207 52 409 30 1099
Cuts at: 4125 5322 5954 513
Size: 1197 632 3074
Fragments arranged by size:
3074 1197 1099 939 632 552 409 324
207 52 30
SwaI ATTT'AAAT
Cuts at: 2508 2508
Size: 8515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2439 2621 2975 3318 4784 6542
Size: 1255 417 442 182 354 343 1466 1758
Cuts at: 6542 6559 6712 6897 7342 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1758 1498 1466 1255 445 442 417 354
343 185 182 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 3470 3580
Size: 274 80 33 51 1443 624 869 110
Cuts at: 3580 3930 5459 6761 8473 96
Size: 350 1529 1302 1712 138
Fragments arranged by size:
1712 1529 1443 1302 869 624 350 274
138 110 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2799 2831 3250 3282 8475 8507 2799
Size: 32 419 32 5193 32 2807
Fragments arranged by size:
5193 2807 419 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8515
XbaI T'CTAG_A
Cuts at: 2485 3280 5420 5851 2485
Size: 795 2140 431 5149
Fragments arranged by size:
5149 2140 795 431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3015 5329 5753 3015
Size: 2314 424 5777
Fragments arranged by size:
5777 2314 424
XhoI C'TCGA_G
Cuts at: 1943 4077 5157 1943
Size: 2134 1080 5301
Fragments arranged by size:
5301 2134 1080
XmaI C'CCGG_G
Cuts at: 1870 5840 1870
Size: 3970 4545
XmnI GAAnn'nnTTC
Cuts at: 2241 3723 4217 5148 6644 2241
Size: 1482 494 931 1496 4112
Fragments arranged by size:
4112 1496 1482 931 494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6323 276
Size: 53 83 186 936 4789 2468
Fragments arranged by size:
4789 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI