Restriction map for
pFN21AE1054
(Circular) (Six-base) MAPSORT of: pFN21AE1054.seq Check: 8584 from: 1 to: 6316
pFN21AE1054 6316 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2657 2657
Size: 6316
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3270 4126 278
Size: 53 83 186 936 1734 856 2468
Fragments arranged by size:
2468 1734 936 856 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3659 1937
Size: 1722 4594
Acc65I G'GTAC_C
Cuts at: 3637 3637
Size: 6316
AclI AA'CG_TT
Cuts at: 1563 4443 4816 1563
Size: 2880 373 3063
Fragments arranged by size:
3063 2880 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 4379 5763 6031 1632
Size: 1301 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 1301 268
AfeI AGC'GCT
Cuts at: 2946 2946
Size: 6316
AflII C'TTAA_G
Cuts at: 820 1017 2752 3365 820
Size: 197 1735 613 3771
Fragments arranged by size:
3771 1735 613 197
AflIII A'CryG_T
Cuts at: 1203 3007 5215 6192 1203
Size: 1804 2208 977 1327
Fragments arranged by size:
2208 1804 1327 977
AgeI A'CCGG_T
Cuts at: 3281 5147 3281
Size: 1866 4450
AhdI GACnn_n'nnGTC
Cuts at: 5045 5045
Size: 6316
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6316
AlwNI CAG_nnn'CTG
Cuts at: 3503 4059 5631 3503
Size: 556 1572 4188
Fragments arranged by size:
4188 1572 556
ApaLI G'TGCA_C
Cuts at: 4373 5529 4373
Size: 1156 5160
ApoI r'AATT_y
Cuts at: 1493 2214 2315 3625 3851 4003 6112 1493
Size: 721 101 1310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 721 226 152 101
AseI AT'TA_AT
Cuts at: 160 1032 4870 160
Size: 872 3838 1606
Fragments arranged by size:
3838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
AvaI C'yCGr_G
Cuts at: 1870 1943 3641 1870
Size: 73 1698 4545
Fragments arranged by size:
4545 1698 73
BamHI G'GATC_C
Cuts at: 3646 4013 3646
Size: 367 5949
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3637 5092 618
Size: 325 217 627 1850 1455 1842
Fragments arranged by size:
1850 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2750 3635 1391
Size: 1359 885 4072
Fragments arranged by size:
4072 1359 885
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4507 4541 1903
Size: 34 2570 34 3678
Fragments arranged by size:
3678 2570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4208 5418 4208
Size: 1210 5106
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6316
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4927 136
Size: 107 122 71 631 605 3255 1525
Fragments arranged by size:
3255 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2793 6311 2793
Size: 3518 2798
BlpI GC'TnA_GC
Cuts at: 3733 3733
Size: 6316
Bme1580I G_kGCm'C
Cuts at: 1790 4377 5533 1790
Size: 2587 1156 2573
Fragments arranged by size:
2587 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2106 1318
Size: 788 5528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3541 4047 5005
Size: 416 53 83 399 24 2124 506 958
Cuts at: 5005 442
Size: 1753
Fragments arranged by size:
2124 1753 958 506 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3489 3489
Size: 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 3026 4976 1239
Size: 862 925 1950 2579
Fragments arranged by size:
2579 1950 925 862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3560 4441 5306 5604 3560
Size: 881 865 298 4272
Fragments arranged by size:
4272 881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4979 882
Size: 617 3480 2219
Fragments arranged by size:
3480 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4107 493
Size: 711 2903 2702
Fragments arranged by size:
2903 2702 711
BsaBI GATnn'nnATC
Cuts at: 6310 6310
Size: 6316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3267 4123 4505 5140
Size: 53 83 186 936 1734 856 382 635
Cuts at: 5140 275
Size: 1451
Fragments arranged by size:
1734 1451 936 856 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2674 3281 4749 5147 5421 5568 5979 2674
Size: 607 1468 398 274 147 411 3011
Fragments arranged by size:
3011 1468 607 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6091
Fragments arranged by size:
6091 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6316
BseYI C'CCAG_C
Cuts at: 3403 5519 6015 3403
Size: 2116 496 3704
Fragments arranged by size:
3704 2116 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2158 1163
Size: 573 422 5321
Fragments arranged by size:
5321 573 422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 3766 4527 5555 1540
Size: 275 186 1285 480 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 480 275 186
BsiHKAI G_wGCw'C
Cuts at: 4377 4462 5533 4377
Size: 85 1071 5160
Fragments arranged by size:
5160 1071 85
BsmI GAATG_Cn'
Cuts at: 2910 3827 3920 2910
Size: 917 93 5306
Fragments arranged by size:
5306 917 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2750 3635 4377 4462 5533 1391
Size: 399 960 885 742 85 1071 2174
Fragments arranged by size:
2174 1071 960 885 742 399 85
BspEI T'CCGG_A
Cuts at: 5979 5979
Size: 6316
BspHI T'CATG_A
Cuts at: 4205 5935 4205
Size: 1730 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 2577 2657 3671 844
Size: 1517 216 80 1014 3489
Fragments arranged by size:
3489 1517 1014 216 80
BsrBI CCG'CTC
Cuts at: 1394 1823 4203 1394
Size: 429 2380 3507
Fragments arranged by size:
3507 2380 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3427 4811 4985 65
Size: 1183 2179 1384 174 1396
Fragments arranged by size:
2179 1396 1384 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3281 4960 5147 1700
Size: 110 1471 1679 187 2869
Fragments arranged by size:
2869 1679 1471 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6316
BssSI C'ACGA_G
Cuts at: 4376 5388 4376
Size: 1012 5304
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6316
BstBI TT'CG_AA
Cuts at: 5120 5120
Size: 6316
BstEII G'GTnAC_C
Cuts at: 1184 2702 1184
Size: 1518 4798
BstXI CCAn_nnnn'nTGG
Cuts at: 3554 3588 3554
Size: 34 6282
BstYI r'GATC_y
Cuts at: 1972 2130 2194 2793 3525 3646 4013 4398 4415
Size: 158 64 599 732 121 367 385 17
Cuts at: 4415 5867 5953 5965 6311 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 732 599 385 367 346 158
121 86 64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 3031 513
Size: 552 972 994 3798
Fragments arranged by size:
3798 994 972 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2206 487
Size: 683 56 980 4597
Fragments arranged by size:
4597 980 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 3832 4626 4646 2125
Size: 1707 794 20 3795
Fragments arranged by size:
3795 1707 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6281
DraI TTT'AAA
Cuts at: 3620 3620
Size: 6316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2392 5323 809
Size: 507 1076 2931 1802
Fragments arranged by size:
2931 1802 1076 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2607 3763 4652
Size: 54 1425 50 275 18 777 1156 889
Cuts at: 4652 6281 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1156 889 777 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 1537
Size: 275 1951 4090
Fragments arranged by size:
4090 1951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4246 1353
Size: 2893 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2822 4903 5277 5423 387
Size: 1064 138 1233 2081 374 146 1280
Fragments arranged by size:
2081 1280 1233 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2933 3026 4379 4976 5763 6031
Size: 393 469 832 93 1353 597 787 268
Cuts at: 6031 1239
Size: 1524
Fragments arranged by size:
1524 1353 832 787 597 469 393 268
93
EcoNI CCTnn'n_nnAGG
Cuts at: 2017 2017
Size: 6316
EcoRI G'AATT_C
Cuts at: 3625 4003 3625
Size: 378 5938
FspI TGC'GCA
Cuts at: 4822 4822
Size: 6316
HaeII r_GCGC'y
Cuts at: 1678 2948 5463 1678
Size: 1270 2515 2531
Fragments arranged by size:
2531 2515 1270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2486 2502 2518 3115
Size: 32 135 32 898 16 16 16 597
Cuts at: 3115 3147 3416 3448 4957 4989 5031 5063 1373
Size: 32 269 32 1509 32 42 32 2626
Fragments arranged by size:
2626 1509 898 597 269 135 42 32
32 32 32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2842 3660 3906 669
Size: 988 281 904 818 246 3079
Fragments arranged by size:
3079 988 904 818 281 246
HindIII A'AGCT_T
Cuts at: 748 2268 748
Size: 1520 4796
HpaI GTT'AAC
Cuts at: 3906 3906
Size: 6316
KpnI G_GTAC'C
Cuts at: 3641 3641
Size: 6316
MfeI C'AATT_G
Cuts at: 2295 2581 3915 2295
Size: 286 1334 4696
Fragments arranged by size:
4696 1334 286
MluI A'CGCG_T
Cuts at: 6192 6192
Size: 6316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2912 5430 5614 1305
Size: 1607 2518 184 2007
Fragments arranged by size:
2518 2007 1607 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4494
Fragments arranged by size:
4494 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2455 2724 3062 4274 4633 4792 518
Size: 648 1289 269 338 1212 359 159 2042
Fragments arranged by size:
2042 1289 1212 648 359 338 269 159
MspA1I CmG'CkG
Cuts at: 1606 3046 3732 4409 5557 5802 1606
Size: 1440 686 677 1148 245 2120
Fragments arranged by size:
2120 1440 1148 686 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 4792
Fragments arranged by size:
4792 972 552
NdeI CA'TA_TG
Cuts at: 387 2721 387
Size: 2334 3982
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6316
NotI GC'GGCC_GC
Cuts at: 3763 3763
Size: 6316
NspI r_CATG'y
Cuts at: 1132 1229 2460 3011 3674 5219 1132
Size: 97 1231 551 663 1545 2229
Fragments arranged by size:
2229 1545 1231 663 551 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6316
PciI A'CATG_T
Cuts at: 3007 5215 3007
Size: 2208 4108
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6316
PfoI T'CCnGG_A
Cuts at: 2012 2012
Size: 6316
PmeI GTTT'AAAC
Cuts at: 3620 3620
Size: 6316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 5898 5930 4385
Size: 32 1481 32 4771
Fragments arranged by size:
4771 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6316
PsiI TTA'TAA
Cuts at: 3886 3886
Size: 6316
PstI C_TGCA'G
Cuts at: 830 2358 2574 3214 3668 830
Size: 1528 216 640 454 3478
Fragments arranged by size:
3478 1528 640 454 216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6316
PvuII CAG'CTG
Cuts at: 3046 3046
Size: 6316
SalI G'TCGA_C
Cuts at: 1936 3658 1936
Size: 1722 4594
SbfI CC_TGCA'GG
Cuts at: 3668 3668
Size: 6316
ScaI AGT'ACT
Cuts at: 3551 4564 6276 3551
Size: 1013 1712 3591
Fragments arranged by size:
3591 1712 1013
SexAI A'CCwGG_T
Cuts at: 2706 2706
Size: 6316
SfcI C'TryA_G
Cuts at: 826 1045 2354 2570 2656 3210 3664 4799 5480
Size: 219 1309 216 86 554 454 1135 681
Cuts at: 5480 5671 826
Size: 191 1471
Fragments arranged by size:
1471 1309 1135 681 554 454 219 216
191 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6316
SmaI CCC'GGG
Cuts at: 1872 3643 1872
Size: 1771 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 3365 3575 4420 5321 5583
Size: 197 926 809 613 210 845 901 262
Cuts at: 5583 820
Size: 1553
Fragments arranged by size:
1553 926 901 845 809 613 262 210
197
SnaBI TAC'GTA
Cuts at: 493 4107 493
Size: 3614 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6316
SphI G_CATG'C
Cuts at: 1132 3674 1132
Size: 2542 3774
SspI AAT'ATT
Cuts at: 5 52 2554 2973 4240 5
Size: 47 2502 419 1267 2081
Fragments arranged by size:
2502 2081 1267 419 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6316
StyI C'CwwG_G
Cuts at: 513 1065 2037 3755 513
Size: 552 972 1718 3074
Fragments arranged by size:
3074 1718 972 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3289 4343 4360 4513 4698 5143 325
Size: 1255 1709 1054 17 153 185 445 1498
Fragments arranged by size:
1709 1498 1255 1054 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2526 2996 3549
Size: 274 80 33 51 1443 549 470 553
Cuts at: 3549 4562 6274 96
Size: 1013 1712 138
Fragments arranged by size:
1712 1443 1013 553 549 470 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6276 6308 6276
Size: 32 6284
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6316
XbaI T'CTAG_A
Cuts at: 3652 3652
Size: 6316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2949 2949
Size: 6316
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6316
XmaI C'CCGG_G
Cuts at: 1870 3641 1870
Size: 1771 4545
XmnI GAAnn'nnTTC
Cuts at: 2405 4445 2405
Size: 2040 4276
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3268 4124 276
Size: 53 83 186 936 1734 856 2468
Fragments arranged by size:
2468 1734 936 856 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI