Restriction map for
pFN21AE1052
(Circular) (Six-base) MAPSORT of: pFN21AE1052.seq Check: 7091 from: 1 to: 5830
pFN21AE1052 5830 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2556 3173 1937
Size: 619 617 4594
Fragments arranged by size:
4594 619 617
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3893 5277 5545 1632
Size: 2261 1384 268 1917
Fragments arranged by size:
2261 1917 1384 268
AfeI AGC'GCT
Cuts at: 2049 2375 2049
Size: 326 5504
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 2727 1166
Size: 1561 4269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 5798
AlwNI CAG_nnn'CTG
Cuts at: 2433 3573 5145 2433
Size: 1140 1572 3118
Fragments arranged by size:
3118 1572 1140
ApaLI G'TGCA_C
Cuts at: 2039 3887 5043 2039
Size: 1848 1156 2826
Fragments arranged by size:
2826 1848 1156
ApoI r'AATT_y
Cuts at: 1493 2148 2423 2633 3139 3365 3517 5626 1493
Size: 655 275 210 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 655 506 275 226 210 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3151 4606 618
Size: 325 217 627 1364 1455 1842
Fragments arranged by size:
1842 1455 1364 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2489 2901 3149 1391
Size: 1098 412 248 4072
Fragments arranged by size:
4072 1098 412 248
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2021 3045 928
Size: 971 122 1024 3713
Fragments arranged by size:
3713 1024 971 122
BbvCI CC'TCA_GC
Cuts at: 2354 2354
Size: 5830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2762 2769 5825 2762
Size: 7 3056 2767
Fragments arranged by size:
3056 2767 7
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2043 3891 5047 1790
Size: 253 1848 1156 2573
Fragments arranged by size:
2573 1848 1156 253
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2802 3005 3561
Size: 416 53 83 399 24 1385 203 556
Cuts at: 3561 4519 442
Size: 958 1753
Fragments arranged by size:
1753 1385 958 556 416 399 203 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 4490 1239
Size: 833 2418 2579
Fragments arranged by size:
2579 2418 833
Bpu10I CC'TnA_GC
Cuts at: 2354 2354
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4493 882
Size: 617 2994 2219
Fragments arranged by size:
2994 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2797 3621 493
Size: 711 1593 824 2702
Fragments arranged by size:
2702 1593 824 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 1178
Size: 30 165 30 1497 30 4078
Fragments arranged by size:
4078 1497 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5830
BseYI C'CCAG_C
Cuts at: 2433 2537 2638 2653 5033 5529 2433
Size: 104 101 15 2380 496 2734
Fragments arranged by size:
2734 2380 496 104 101 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 2866 1163
Size: 573 723 407 4127
Fragments arranged by size:
4127 723 573 407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2724 2880 3891 3976 5047 2043
Size: 681 156 1011 85 1071 2826
Fragments arranged by size:
2826 1071 1011 681 156 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
BsmBI CGTCTCn'nnnn_
Cuts at: 3041 3041
Size: 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2489 2724 2880 2901 3149 3891
Size: 399 253 446 235 156 21 248 742
Cuts at: 3891 3976 5047 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 253 248 235
156 85 21
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 2205 3719 5449 2205
Size: 1514 1730 2586
Fragments arranged by size:
2586 1730 1514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3007 3890 4902 3007
Size: 883 1012 3935
Fragments arranged by size:
3935 1012 883
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5830
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 2596 1184
Size: 1412 4418
BstYI r'GATC_y
Cuts at: 1972 2649 2762 2769 2925 3160 3527 3912 3929
Size: 677 113 7 156 235 367 385 17
Cuts at: 3929 5381 5467 5479 5825 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 677 385 367 346 235 156
113 86 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5278
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 3075 3346 4140 4160 3075
Size: 271 794 20 4745
Fragments arranged by size:
4745 794 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2605 4837 809
Size: 507 1289 2232 1802
Fragments arranged by size:
2232 1802 1289 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 2380 2610
Size: 54 1425 50 275 18 235 315 230
Cuts at: 2610 3277 4166 5795 8
Size: 667 889 1629 43
Fragments arranged by size:
1629 1425 889 667 315 275 235 230
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3760 1353
Size: 2407 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2762 4417 4791 4937 387
Size: 1064 138 1173 1655 374 146 1280
Fragments arranged by size:
1655 1280 1173 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 3893 4490 5277 5545 1239
Size: 393 440 1821 597 787 268 1524
Fragments arranged by size:
1821 1524 787 597 440 393 268
EcoRI G'AATT_C
Cuts at: 2423 2633 3139 3517 2423
Size: 210 506 378 4736
Fragments arranged by size:
4736 506 378 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 5830
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2051 2377 4977 1678
Size: 373 326 2600 2531
Fragments arranged by size:
2600 2531 373 326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2754 2786 4471
Size: 32 135 32 584 32 566 32 1685
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1685 584 566 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2388 2398 3174 3420 669
Size: 988 281 450 10 776 246 3079
Fragments arranged by size:
3079 988 776 450 281 246 10
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2067 10
Size: 54 1425 343 235 3773
Fragments arranged by size:
3773 1425 343 235 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2727 2873 3032 3788 4147 4306 518
Size: 648 1561 146 159 756 359 159 2042
Fragments arranged by size:
2042 1561 756 648 359 159 159 146
MspA1I CmG'CkG
Cuts at: 1606 2430 2451 2788 3246 3923 5071 5316 1606
Size: 824 21 337 458 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 458 337 245 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5278
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 3188 4733 1132
Size: 97 1959 1545 2229
Fragments arranged by size:
2229 1959 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2933 1348
Size: 1585 4245
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2660 2731 1108
Size: 1552 71 4207
Fragments arranged by size:
4207 1552 71
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 3899 3931 5412 5444 2900
Size: 32 967 32 1481 32 3286
Fragments arranged by size:
3286 1481 967 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2393 1613
Size: 780 5050
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2511 2543 2511
Size: 32 5798
PstI C_TGCA'G
Cuts at: 830 2450 2465 2744 3182 830
Size: 1620 15 279 438 3478
Fragments arranged by size:
3478 1620 438 279 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2430 2788 2430
Size: 358 5472
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2277 2446 2461 2740 3178 4313 4994
Size: 219 1232 169 15 279 438 1135 681
Cuts at: 4994 5185 826
Size: 191 1471
Fragments arranged by size:
1471 1232 1135 681 438 279 219 191
169 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2953 1517
Size: 1436 4394
StyI C'CwwG_G
Cuts at: 513 1065 2643 3269 513
Size: 552 1578 626 3074
Fragments arranged by size:
3074 1578 626 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2298 2425 3857 3874 4027 4212 4657
Size: 1255 718 127 1432 17 153 185 445
Cuts at: 4657 325
Size: 1498
Fragments arranged by size:
1498 1432 1255 718 445 185 153 127
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4076 5788 96
Size: 274 80 33 51 1443 2099 1712 138
Fragments arranged by size:
2099 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 2197 1316
Size: 881 4949
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI