Restriction map for
pFN21AE1050
(Circular) (Six-base) MAPSORT of: pFN21AE1050.seq Check: 203 from: 1 to: 6133
pFN21AE1050 6133 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3104 3104
Size: 6133
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 4196 5580 5848 1632
Size: 800 1764 1384 268 1917
Fragments arranged by size:
1917 1764 1384 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 5032 6009 1203
Size: 3829 977 1327
Fragments arranged by size:
3829 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 3098 1166
Size: 1932 4201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 3390 3876 5448 3390
Size: 486 1572 4075
Fragments arranged by size:
4075 1572 486
ApaI G_GGCC'C
Cuts at: 2016 2459 2586 2597 2736 2941 2016
Size: 443 127 11 139 205 5208
Fragments arranged by size:
5208 443 205 139 127 11
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 3442 3668 3820 5929 1493
Size: 1949 226 152 2109 1697
Fragments arranged by size:
2109 1949 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 2590 3458 1870
Size: 73 143 504 868 4545
Fragments arranged by size:
4545 868 504 143 73
BamHI G'GATC_C
Cuts at: 3051 3463 3830 3051
Size: 412 367 5354
Fragments arranged by size:
5354 412 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2740 3454 4909 618
Size: 325 217 627 294 659 714 1455 1842
Fragments arranged by size:
1842 1455 714 659 627 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2016 2046 2056 2247 2379 2408 2459 2586
Size: 625 30 10 191 132 29 51 127
Cuts at: 2586 2597 2736 2941 3408 3452 1391
Size: 11 139 205 467 44 4072
Fragments arranged by size:
4072 625 467 205 191 139 132 127
51 44 30 29 11 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 2573 3123
Size: 107 122 71 631 605 348 553 550
Cuts at: 3123 4744 136
Size: 1621 1525
Fragments arranged by size:
1621 1525 631 605 553 550 348 122
107 71
BglII A'GATC_T
Cuts at: 6128 6128
Size: 6133
BlpI GC'TnA_GC
Cuts at: 2303 3550 2303
Size: 1247 4886
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2459 2586 2597 2736 2745 2941 3366
Size: 226 443 127 11 139 9 196 425
Cuts at: 3366 4194 5350 1790
Size: 828 1156 2573
Fragments arranged by size:
2573 1156 828 443 425 226 196 139
127 11 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3864 4822 442
Size: 416 53 83 399 24 2447 958 1753
Fragments arranged by size:
2447 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2132 2772 2132
Size: 640 5493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2213 2448 2742 3032 4793 1239
Size: 863 111 235 294 290 1761 2579
Fragments arranged by size:
2579 1761 863 294 290 235 111
Bpu10I CC'TnA_GC
Cuts at: 2747 3228 2747
Size: 481 5652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2698 4796 882
Size: 617 1199 2098 2219
Fragments arranged by size:
2219 2098 1199 617
BsaAI yAC'GTr
Cuts at: 493 1204 2908 3001 3027 3924 493
Size: 711 1704 93 26 897 2702
Fragments arranged by size:
2702 1704 897 711 93 26
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2286 3940 4322 4957
Size: 53 83 186 936 753 1654 382 635
Cuts at: 4957 275
Size: 1451
Fragments arranged by size:
1654 1451 936 753 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2185 3048 4566 4964 5238 5385 5796 2185
Size: 863 1518 398 274 147 411 2522
Fragments arranged by size:
2522 1518 863 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2844 2874 1178
Size: 30 165 30 1441 30 4437
Fragments arranged by size:
4437 1441 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2733 2989 3257 3313 3317 1186
Size: 1547 256 268 56 4 4002
Fragments arranged by size:
4002 1547 268 256 56 4
BseYI C'CCAG_C
Cuts at: 2437 2500 2687 2816 2876 2940 3033 5336 5832
Size: 63 187 129 60 64 93 2303 496
Cuts at: 5832 2437
Size: 2738
Fragments arranged by size:
2738 2303 496 187 129 93 64 63
60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2730 3032 3408 4194 4279 5350 2730
Size: 302 376 786 85 1071 3513
Fragments arranged by size:
3513 1071 786 376 302 85
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
BsmBI CGTCTCn'nnnn_
Cuts at: 2448 2448
Size: 6133
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2046 2056 2247 2379 2408 2459
Size: 399 226 30 10 191 132 29 51
Cuts at: 2459 2586 2597 2730 2736 2745 2941 3032 3366
Size: 127 11 133 6 9 196 91 334
Cuts at: 3366 3408 3452 4194 4279 5350 1391
Size: 42 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 226 196 191
133 132 127 91 85 51 44 42
30 29 11 10 9 6
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3104 3488 844
Size: 2260 384 3489
Fragments arranged by size:
3489 2260 384
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 4020 1394
Size: 429 611 1586 3507
Fragments arranged by size:
3507 1586 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4628 4802 65
Size: 1183 3380 174 1396
Fragments arranged by size:
3380 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2098 2200 4777 4964 1700
Size: 110 288 102 2577 187 2869
Fragments arranged by size:
2869 2577 288 187 110 102
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3381 794
Size: 2587 3546
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstXI CCAn_nnnn'nTGG
Cuts at: 2843 2843
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 3051 3156 3463 3830 4215 4232 5684 5770
Size: 1079 105 307 367 385 17 1452 86
Cuts at: 5770 5782 6128 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1079 385 367 346 307 105
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2058 2058
Size: 6133
BtgI C'CryG_G
Cuts at: 513 1065 2077 2104 2218 2967 3112 513
Size: 552 1012 27 114 749 145 3534
Fragments arranged by size:
3534 1012 749 552 145 114 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2168 3160 3649 4443 4463 2168
Size: 992 489 794 20 3838
Fragments arranged by size:
3838 992 794 489 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2833 2950
Size: 54 1425 50 275 18 486 517 117
Cuts at: 2950 3003 3580 4469 6098 8
Size: 53 577 889 1629 43
Fragments arranged by size:
1629 1425 889 577 517 486 275 117
54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2704 2710 4063 1353
Size: 1351 6 1353 3423
Fragments arranged by size:
3423 1353 1351 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3406 3406
Size: 6133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2213 2432 2448 2742 3032 4196
Size: 393 470 111 219 16 294 290 1164
Cuts at: 4196 4793 5580 5848 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1164 787 597 470 393 294 290
268 219 111 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2610 3231 2610
Size: 621 5512
EcoO109I rG'GnC_Cy
Cuts at: 2012 2173 2214 2263 2455 2456 2583 2594 2633
Size: 161 41 49 192 1 127 11 39
Cuts at: 2633 2733 2012
Size: 100 5412
Fragments arranged by size:
5412 192 161 127 100 49 41 39
11 1
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 3390 5280 1678
Size: 1712 1890 2531
Fragments arranged by size:
2531 1890 1712
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2610 2642 4774 4806 4848
Size: 32 135 32 1038 32 2132 32 42
Cuts at: 4848 4880 1373
Size: 32 2626
Fragments arranged by size:
2626 2132 1038 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3723 669
Size: 988 281 1539 246 3079
Fragments arranged by size:
3079 1539 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6133
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5247 5431 1305
Size: 3942 184 2007
Fragments arranged by size:
3942 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2835 3005 10
Size: 54 1425 343 1003 170 3138
Fragments arranged by size:
3138 1425 1003 343 170 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 4091 4450 4609 518
Size: 648 1932 993 359 159 2042
Fragments arranged by size:
2042 1932 993 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 2820 3114 3355 3549 4226 5374 5619
Size: 473 741 294 241 194 677 1148 245
Cuts at: 5619 1606
Size: 2120
Fragments arranged by size:
2120 1148 741 677 473 294 245 241
194
NaeI GCC'GGC
Cuts at: 2202 2202
Size: 6133
NcoI C'CATG_G
Cuts at: 513 1065 2218 513
Size: 552 1153 4428
Fragments arranged by size:
4428 1153 552
NdeI CA'TA_TG
Cuts at: 387 2371 387
Size: 1984 4149
NgoMIV G'CCGG_C
Cuts at: 2200 2200
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 2934 3491 5036 1132
Size: 97 1705 557 1545 2229
Fragments arranged by size:
2229 1705 1545 557 97
PasI CC'CwG_GG
Cuts at: 1348 2130 2177 1348
Size: 782 47 5304
Fragments arranged by size:
5304 782 47
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PfoI T'CCnGG_A
Cuts at: 3042 3399 3042
Size: 357 5776
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PmlI CAC'GTG
Cuts at: 2908 3027 2908
Size: 119 6014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2214 2214
Size: 6133
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PspOMI G'GGCC_C
Cuts at: 2012 2455 2582 2593 2732 2937 2012
Size: 443 127 11 139 205 5208
Fragments arranged by size:
5208 443 205 139 127 11
PstI C_TGCA'G
Cuts at: 830 2254 2707 3386 3485 830
Size: 1424 453 679 99 3478
Fragments arranged by size:
3478 1424 679 453 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
SacI G_AGCT'C
Cuts at: 3408 3408
Size: 6133
SacII CC_GC'GG
Cuts at: 2080 3115 2080
Size: 1035 5098
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SanDI GG'GwC_CC
Cuts at: 2214 2214
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 2707 3485 2707
Size: 778 5355
ScaI AGT'ACT
Cuts at: 2226 2828 4381 6093 2226
Size: 602 1553 1712 2266
Fragments arranged by size:
2266 1712 1553 602
SfcI C'TryA_G
Cuts at: 826 1045 2250 2641 2703 3382 3481 4616 5297
Size: 219 1205 391 62 679 99 1135 681
Cuts at: 5297 5488 826
Size: 191 1471
Fragments arranged by size:
1471 1205 1135 681 679 391 219 191
99 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 2088 2592 3460 1872
Size: 216 504 868 4545
Fragments arranged by size:
4545 868 504 216
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 2576 3074 3380 1517
Size: 1059 498 306 4270
Fragments arranged by size:
4270 1059 498 306
StyI C'CwwG_G
Cuts at: 513 1065 2218 3572 513
Size: 552 1153 1354 3074
Fragments arranged by size:
3074 1354 1153 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 2376 2569 4160 4177 4330 4515
Size: 1255 617 179 193 1591 17 153 185
Cuts at: 4515 4960 325
Size: 445 1498
Fragments arranged by size:
1591 1498 1255 617 445 193 185 179
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 2826 4379
Size: 274 80 33 51 1443 247 602 1553
Cuts at: 4379 6091 96
Size: 1712 138
Fragments arranged by size:
1712 1553 1443 602 274 247 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 6093 6125 2844
Size: 32 3217 32 2852
Fragments arranged by size:
3217 2852 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 2086 2590 3458 1870
Size: 216 504 868 4545
Fragments arranged by size:
4545 868 504 216
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspXI PsrI PvuII RsrII
SapI SexAI SfiI SfoI SrfI SwaI XcmI